Background: The potassium voltage-gated channel subfamily Q member 1 (KCNQ1) gene has recently received much attention as a candidate susceptibility gene for type 2 diabetes mellitus, especially in Asian populations. We previously reported that Kcnq1 mutant mice exhibit reduced insulin secretion and hyperglycemia due to a decrease in pancreatic β-cell mass. Through in vivo and in vitro analyses, we ascertained that this mechanism is the result of the downregulation of the non-coding RNA ‘Kcnq1ot1,’ which is expressed in the paternal allele of the Kcnq1 gene region, causing an increase in the expression of the cell cycle inhibitor cyclin dependent kinase inhibitor 1C (Cdkn1c). It was found that decreased Kcnq1ot1 expression resulted in pancreatic β-cell failure; however, the degree of pancreatic β-cell volume reduction was not severe. Methods: We induced obesity in Kcnq1ot1 truncation mice by feeding them a high-fat diet and evaluated pancreatic β-cell mass. Results: In the present study, we reveal that CCAAT/enhancer binding protein beta (C/EBPβ), which is expressed at higher levels in pancreatic β-cells in obese individuals, further increases the expression of Cdkn1c, which is upregulated by the Kcnq1 gene mutation. We found that simultaneous Cdkn1c hypomethylation and C/EBPβ overexpression in pancreatic β-cells causes a synergistic decrease in pancreatic β-cell mass. Conclusion This finding suggests that the synergistic effect of genetic factors such as Kcnq1 gene mutations and environmental factors such as obesity and overeating, which lead to increased expression of C/EBPβ, contribute to the regulation of pancreatic β-cell mass. This study is the first to show that the Kcnq1 gene is related to pancreatic β-cell mass through genetic-environment interactions.

Green tea catechins are well known to be one of polyphenols, and its regular ingestion induces body fat reduction in obese individuals. Cocoa polyphenols of high-cocoa chocolate can also improve arterial stiffness. However, it is unclear whether green tea catechins improve body fat and arterial stiffness even in healthy young adults. Therefore, this study aimed to examine the effects of regular green tea intake on body fat and arterial stiffness in young adults. This randomized, controlled, parallel-group intervention study included 53 Japanese college students (mean age, 21.1 ± 0.1; men, n = 40; women, n = 13). They were randomly divided into three groups: control group (n = 14), intervention I group (n = 19), and intervention II group (n = 20). The participants ingested 500 ml/day of commercially available natural water (0 mg of catechin/day) or green tea (intervention I group, 200 mg of catechin/day; intervention II group, 400 mg of catechin/day) for 4 weeks. After 4 weeks of intervention period, no significant changes in body weight, body mass index, pulse wave velocity, and cardio-ankle vascular index were observed in all groups. However, body fat and carbon dioxide output significantly reduced only in the intervention II group. With the level of catechin concentrations increasing, significant decreasing trends were found in body fat changes and respiratory exchange ratio changes. Therefore, these findings suggest that four weeks of regular green tea intake would reduce body fat, but not arterial stiffness, in young adults.

This retrospective observational study aimed to examine the effects of playing Pokémon GO on daily steps of male college students. Twenty-five Japanese male college students (20 ± 1 years) were assigned to Control group (C, n = 11) or Pokémon GO group (P, n = 14) based on their playing Pokémon GO or not. Daily step levels were obtained from the health care app of Apple iPhone from September 2016 to October 2016. In P group, the data for the 4 weeks to determine baseline values and for the 4 weeks of playing Pokémon GO were analyzed. The steps data of C group were also analyzed in the same period of the total 8 weeks. There were no significant differences in steps at baseline level between both groups. In addition, no significant time-course changes in steps were observed in C group. In contrast, steps in P group significantly increased from baseline 8,368 ± 544 steps/day to week 1 10,028 ± 617 steps/day (P < 0.01). The changes in steps from baseline to week 1 were significantly greater in P group than in C group (1,671 ± 345 vs. -81 ± 582, P < 0.01). However, the increased steps returned to baseline level by week 2, and the values did not increase again. Therefore, our findings indicate that playing Pokémon GO increases daily steps only during one week in Japanese male college students.

Adolescent ; Adult ; Brain Neoplasms ; surgery ; Female ; Hemangioma, Cavernous ; surgery ; Humans ; Male ; Young Adult

This study aimed to clarify the effects of a lifestyle intervention by the concurrent use of an activity monitor and Twitter on daily physical activity. Twenty-one healthy males and females (37 ± 13 yrs) were randomly assigned to Normal intervention group (N group, n = 10) or Twitter intervention group (T group, n = 11). In both groups, the intervention period was 6 weeks. Participants in N group wore an activity monitor (Lifecorder EX). Meanwhile, participants in T group were asked to tweet about their daily steps or physical activity in addition to wearing an activity monitor, and also an observer read through the tweet from each participant and commented about physical activity. There were no significant differences in daily physical activity (i.e., steps and amount of physical activity (PA)) at week 1 between both groups. In addition, no significant time-course changes in steps and amount of PA were observed in N group. In contrast, steps in T group were gradually increased from week 1 8,542 ± 3,158 steps/day to week 6 12,700 ± 3,935 steps/day (P < 0.01). Amount of PA in T group was also gradually increased from week 1 2.5 ± 1.2 METs·hour/day to week 6 4.6 ± 2.3 METs·hour/day (P < 0.01). Therefore, these findings indicate that the lifestyle intervention by the concurrent use of an activity monitor and Twitter could effectively induce an increase in daily physical activity compared with the intervention using only an activity monitor.

This study aimed to examine a new walking method to fulfill the Physical Activity Reference for Health Promotion in Japan in a shorter time, which can be performed as a non-exercise activity (NEA) such as walking during commuting. Eleven healthy subjects performed normal walking (NW) and knee-up walking (KU) for eight minutes without load (0%) and with loads of 5, 10, and 20% of body mass in a backpack carried on the back (NW-0, 5, 10, and 20; KU-0, 5, 10, and 20) on a treadmill at a speed of 5 km/h at 0% tilt. Oxygen uptake, blood lactate levels, and ratings of perceived exertion (RPE) were measured in the last two minutes of each walking stage. Metabolic equivalents (METs) were also calculated from the resultant oxygen uptake. METs in NW-20 and all weight conditions of KUs were significantly greater (P < 0.01) than those in NW-0 (3.8 ± 0.1 METs). RPE in both KU-10 and KU-20 was above 15 (i.e., indicating Hard). Blood lactate levels in the KU-20 (4.5 ± 0.7 mmol/L) were above the Onset of Blood Lactate Accumulation. On the other hand, NW-20, KU-0, and KU-5 were physical activity level of moderate- to vigorous- intensity that could be performed as a NEA. These data suggest that our new walking methods such as NW-20, KU-0, and KU-5 can satisfy the Physical Activity Reference in a shorter time rather than normal walking, and could be continuously performed in daily life.

This study aimed at clarifying the effects of a lifestyle intervention using an activity monitor with game functions on daily physical activity. A 12-week randomized crossover study was conducted in 23 healthy volunteers (31 ± 11 yrs). The subjects were randomly assigned to Group A (a 6-week game intervention and then a 6-week normal intervention) and Group B (a 6-week normal intervention and then a 6-week game intervention). In the game intervention period (G), the subjects wore two different activity monitors, namely a normal activity monitor (Lifecorder EX) and an activity monitor with game functions (Yuuhokei). On the other hand, in the normal intervention period (N), the subjects wore only a normal activity monitor. Daily steps were significantly higher in the G than in the N (G: 10417 ± 2358 vs. N: 8744 ± 2180 steps/day, P < 0.001). Amount of physical activity (i.e., daily total METs · hour = Ex/day) at the intensity of 3 METs or more was significantly higher in the G than in the N (G: 3.1 ± 0.9 vs. N: 2.4 ± 0.8 Ex/day, P < 0.001). Our results indicate that the lifestyle intervention using an activity monitor with game functions would more effectively increase daily physical activity such as steps and physical activity amount, than a normal intervention using an activity monitor. These findings therefore have important implications for reaching more easily the goals (i.e., 23 Ex/week or 10,000 steps/day) and the use of an activity monitor with game functions in the field of exercise or physical activity instructions.

PURPOSE: To investigate the efficacy of tazobactam/piperacillin (TAZ/PIPC) plus levofloxacin (LVFX) as a prophylactic administration in transrectal prostate biopsy (TPBX). MATERIALS AND METHODS: We investigated 201 consecutive patients who underwent TPBX in one Japanese hospital during the period of 2009-2010. The patients received TAZ/PIPC 4.5 g i.v. once just before and 3 hours after TPBX, plus oral LVFX 300 mg or 500 mg daily for 3 days. We examined the infectious adverse events and laboratory data (serum white blood cell [WBC] count and C-reactive protein [CRP]) before and 1 day after TPBX. RESULTS: Only one patient (0.50%) in 201 cases had febrile complications after TPBX. Serum WBC and CRP did not rise significantly on the day after TPBX compared with before TPBX (p>0.05). There was no significant difference in the rise of serum WBC and CRP before and after TPBX in the comparison of LVFX 500 mg with LVFX 300 mg in the TAZ/PIPC plus LVFX regimen. CONCLUSIONS: TAZ/PIPC plus LVFX can be considered as a prophylactic regimen for preventing infectious complications in TPBX.
Antibiotic Prophylaxis ; Asian Continental Ancestry Group ; beta-Lactamases ; Biopsy ; C-Reactive Protein ; Fluoroquinolones ; Humans ; Leukocytes ; Ofloxacin ; Penicillins ; Prostate

There were 651 deliveries and 4 maternal deaths at Christian Hospital Chandraghona between October 2000 and August 2001. Eleven eclampsia patients were admitted and the mean age was 22.1 years. Ten patients were primipara and 1 patient was multipara. Among those eclampsia cases, 2 maternal deaths were observed and 10 patients delivered babies (8 live babies and 2 stillbirths). Delivery methods were 8 normal deliveries, 1 forceps delivery and 1 cesarean section. Ten patients had never consulted a doctor before eclampsia occurred. Eclampsia occurred at the third trimester of pregnancy in 9 patients and after delivery in 2 patients. Nine patients came to the hospital within 5 hours after the onset of eclampsia. Those who came late to the hospital progressed to be maternal death. To reduce maternal death, it is necessary to promote antenatal checkup and provide education about obstetrical complications at the grassroots level.

[Purpose] We report a case treated by acupuncture in a health care facility for the elderly to evaluate the effect of acupuncture on severe pulmonary emphysema.
[Case and methods] A 88 year-old man visited Meiji University of Oriental Medicine Hospital with dyspnea in March 2000 and was diagnosed with pulmonary emphysema. He was hospitalized in October 2002 because his chronic respiratory failure was worsening : oxygen therapy was started. After discharge from hospital, he entered a health care facility for the elderly and acupuncture treatment for lumbago was initiated. Thereafter, acupuncture treatment for dyspnea and stiff shoulder started. He was determined to be grade V according to Fletcher-Hugh-Jones classification of dyspnea. Spirometry of the patient before acupuncture for dyspnea showed 88.0 % in %VC, 38.2 % in FEV 1 %, and 30 % in %FEV 1, and the patient was diagnosed with chronic obstructive pulmonary disease of serious grade. Acupuncture treatment was performed once or twice a week, and acupuncture treatment for dyspnea was performed 21 times (total of 33 treatments).
[Results] Subjective symptoms of lumbago, stagger of the legs, and shoulder stiffness were evaluated with Numerical Scale or Pain Scale. The state of dyspnea was evaluated with Numerical Scale and Borg Scale, and exercise tolerance was evaluated with 6 min of walking. After 33 acupuncture treatments, dyspnea on exertion, exercise tolerance, and the findings of spirometry were not improved. But dyspnea on rest had disappeared with improvement in lumbago, stagger of the legs, and shoulder stiffness. These results indicate that acupuncture as a general treatment is effective for the improvement of dyspnea, especially in elderly patients whose general state of health is not good because of restriction in their ability to exercise in the course of daily life due to dyspnea of exercise such as lumbago, stagger of the legs, and shoulder stiffness.