In breast cancer, sentinel lymph nodes (SLNs) represent the first site of interaction between tumor-derived antigens and the host immune system. However, descriptive data on immune cell subsets within the SLN remains limited. This exploratory study evaluated immune cell populations in SLN samples from women with invasive breast cancer (IBC) using flow cytometry, and assessed their distribution according to clinicopathological characteristics.SNL scrapings were collected from 22 patients with IBC. SNL cells were evaluated using flow cytometry, and the results were compared with clinical and pathological variables, such as tumor subtype, axillary status, lymphovascular invasion (LVI), histological grades, and expression of estrogen receptors (ERs) and human epidermal growth factor receptor 2 (HER2). The frequencies of monocytes and neutrophils were not significantly correlated with the clinical variables analyzed. In patients with LVI, there was an increase in the frequency of CD4+ T cells and programmed death-1 (PD-1) expression in CD8+ T-cells.Furthermore, a reduction in central memory CD4+ T cells and an increase in terminal effector memory CD4+ T cells were observed in patients with histological grade III disease compared to those with histological grade I disease. It was also observed that in patients expressing ERs and HER2, there was an increase in PD-1 and programmed death-ligand 1 expression in CD8+ T-cells. These findings provide a descriptive overview of immune cell distribution in SLNs according to their clinicopathological features. These results are preliminary and hypothesis-generating, supporting the need for larger longitudinal studies to further characterize the immune profiles within tumor-draining lymph nodes.

Objective: Several studies have highlighted the toxic potential of bisphenol A (BPA), however, BPA release from orthopedic devices remains poorly investigated.Therefore, this study aimed to evaluate BPA levels in the saliva of children treated using Haas expanders. Methods: Twenty-two children of both sexes aged 6–10 years who required rapid maxillary expansion were recruited. One week after placement of elastics to separate the permanent molars, orthodontic bands were adapted, and maxillary impressions were obtained using alginate impression material. Haas expanders were fabricated using a standardized amount of acrylic resin. The bands were cemented using Transbond Plus Light Cure Band (3M).Saliva samples were collected at five time points: before (T0) and 30 minutes (T1), 24 hours (T2), 1 week (T3), and 1 month (T4) after Haas expander installation.BPA levels were measured using ultra-high-performance liquid chromatography coupled with Tandem Mass Spectrometry. The results were evaluated using oneway analysis of variance with Tukey’s post-hoc test (alpha = 5%). Results: BPA levels were below the recommended tolerable daily intake (TDI) at all timepoints;however, salivary BPA levels at T1 (70.324 ng/mL ± 37.05) and at T2 (18.015 ng/mL ± 11.22) were significantly higher compared to that at T0 (0.475 ng/mL ± 0.27) (P < 0.05). Conclusions Salivary BPA levels significantly increased 30 minutes and 24 hours after Haas expander installation and return to baseline values after 1 week. BPA levels did not exceed the TDI, suggesting that the use of Haas expanders may be considered safe concerning BPA exposure in children.

Objective: Several studies have highlighted the toxic potential of bisphenol A (BPA), however, BPA release from orthopedic devices remains poorly investigated.Therefore, this study aimed to evaluate BPA levels in the saliva of children treated using Haas expanders. Methods: Twenty-two children of both sexes aged 6–10 years who required rapid maxillary expansion were recruited. One week after placement of elastics to separate the permanent molars, orthodontic bands were adapted, and maxillary impressions were obtained using alginate impression material. Haas expanders were fabricated using a standardized amount of acrylic resin. The bands were cemented using Transbond Plus Light Cure Band (3M).Saliva samples were collected at five time points: before (T0) and 30 minutes (T1), 24 hours (T2), 1 week (T3), and 1 month (T4) after Haas expander installation.BPA levels were measured using ultra-high-performance liquid chromatography coupled with Tandem Mass Spectrometry. The results were evaluated using oneway analysis of variance with Tukey’s post-hoc test (alpha = 5%). Results: BPA levels were below the recommended tolerable daily intake (TDI) at all timepoints;however, salivary BPA levels at T1 (70.324 ng/mL ± 37.05) and at T2 (18.015 ng/mL ± 11.22) were significantly higher compared to that at T0 (0.475 ng/mL ± 0.27) (P < 0.05). Conclusions Salivary BPA levels significantly increased 30 minutes and 24 hours after Haas expander installation and return to baseline values after 1 week. BPA levels did not exceed the TDI, suggesting that the use of Haas expanders may be considered safe concerning BPA exposure in children.

Objective: Several studies have highlighted the toxic potential of bisphenol A (BPA), however, BPA release from orthopedic devices remains poorly investigated.Therefore, this study aimed to evaluate BPA levels in the saliva of children treated using Haas expanders. Methods: Twenty-two children of both sexes aged 6–10 years who required rapid maxillary expansion were recruited. One week after placement of elastics to separate the permanent molars, orthodontic bands were adapted, and maxillary impressions were obtained using alginate impression material. Haas expanders were fabricated using a standardized amount of acrylic resin. The bands were cemented using Transbond Plus Light Cure Band (3M).Saliva samples were collected at five time points: before (T0) and 30 minutes (T1), 24 hours (T2), 1 week (T3), and 1 month (T4) after Haas expander installation.BPA levels were measured using ultra-high-performance liquid chromatography coupled with Tandem Mass Spectrometry. The results were evaluated using oneway analysis of variance with Tukey’s post-hoc test (alpha = 5%). Results: BPA levels were below the recommended tolerable daily intake (TDI) at all timepoints;however, salivary BPA levels at T1 (70.324 ng/mL ± 37.05) and at T2 (18.015 ng/mL ± 11.22) were significantly higher compared to that at T0 (0.475 ng/mL ± 0.27) (P < 0.05). Conclusions Salivary BPA levels significantly increased 30 minutes and 24 hours after Haas expander installation and return to baseline values after 1 week. BPA levels did not exceed the TDI, suggesting that the use of Haas expanders may be considered safe concerning BPA exposure in children.

Objective: Several studies have highlighted the toxic potential of bisphenol A (BPA), however, BPA release from orthopedic devices remains poorly investigated.Therefore, this study aimed to evaluate BPA levels in the saliva of children treated using Haas expanders. Methods: Twenty-two children of both sexes aged 6–10 years who required rapid maxillary expansion were recruited. One week after placement of elastics to separate the permanent molars, orthodontic bands were adapted, and maxillary impressions were obtained using alginate impression material. Haas expanders were fabricated using a standardized amount of acrylic resin. The bands were cemented using Transbond Plus Light Cure Band (3M).Saliva samples were collected at five time points: before (T0) and 30 minutes (T1), 24 hours (T2), 1 week (T3), and 1 month (T4) after Haas expander installation.BPA levels were measured using ultra-high-performance liquid chromatography coupled with Tandem Mass Spectrometry. The results were evaluated using oneway analysis of variance with Tukey’s post-hoc test (alpha = 5%). Results: BPA levels were below the recommended tolerable daily intake (TDI) at all timepoints;however, salivary BPA levels at T1 (70.324 ng/mL ± 37.05) and at T2 (18.015 ng/mL ± 11.22) were significantly higher compared to that at T0 (0.475 ng/mL ± 0.27) (P < 0.05). Conclusions Salivary BPA levels significantly increased 30 minutes and 24 hours after Haas expander installation and return to baseline values after 1 week. BPA levels did not exceed the TDI, suggesting that the use of Haas expanders may be considered safe concerning BPA exposure in children.

Objective: Several studies have highlighted the toxic potential of bisphenol A (BPA), however, BPA release from orthopedic devices remains poorly investigated.Therefore, this study aimed to evaluate BPA levels in the saliva of children treated using Haas expanders. Methods: Twenty-two children of both sexes aged 6–10 years who required rapid maxillary expansion were recruited. One week after placement of elastics to separate the permanent molars, orthodontic bands were adapted, and maxillary impressions were obtained using alginate impression material. Haas expanders were fabricated using a standardized amount of acrylic resin. The bands were cemented using Transbond Plus Light Cure Band (3M).Saliva samples were collected at five time points: before (T0) and 30 minutes (T1), 24 hours (T2), 1 week (T3), and 1 month (T4) after Haas expander installation.BPA levels were measured using ultra-high-performance liquid chromatography coupled with Tandem Mass Spectrometry. The results were evaluated using oneway analysis of variance with Tukey’s post-hoc test (alpha = 5%). Results: BPA levels were below the recommended tolerable daily intake (TDI) at all timepoints;however, salivary BPA levels at T1 (70.324 ng/mL ± 37.05) and at T2 (18.015 ng/mL ± 11.22) were significantly higher compared to that at T0 (0.475 ng/mL ± 0.27) (P < 0.05). Conclusions Salivary BPA levels significantly increased 30 minutes and 24 hours after Haas expander installation and return to baseline values after 1 week. BPA levels did not exceed the TDI, suggesting that the use of Haas expanders may be considered safe concerning BPA exposure in children.

Patients with anteroposterior maxillary deficiency and mandibular prognathism associated with a long-face pattern exhibit reduced anterior and lateral projection of the zygomaticomaxillary complex. Several techniques have been combined with orthognathic surgery to enhance the volume and definition of this region, such as zygomatic osteotomy. Two female patients, aged 22 (Case A) and 29 (Case B), with class II (Case A) and III (Case B) malocclusion, respectively, complained about facial aesthetics due to a long face and lack of malar projection. Le Fort I osteotomy to advance the maxilla, bilateral mandibular ramus osteotomy to set back the mandible, mentoplasty for advancement and width reduction, and zygomatic osteotomy were erformed. The patients underwent computed tomography scans, and superimposed images indicated that a volumetric increase in the malar region was achieved through the anterolateral movement of the zygomatic body. Improvements were noted in the paranasal region (Case A), better alignment of the upper and lower lips, the chin region, and an improved chin-neckline ratio (in both cases). The patients were satisfied with the results and are under follow-up. In summary, zygomatic osteotomy combined with orthognathic surgery proved to be a predictable and safe strategy for increasing malar prominence, with satisfactory aesthetic and functional outcomes.

Patients with anteroposterior maxillary deficiency and mandibular prognathism associated with a long-face pattern exhibit reduced anterior and lateral projection of the zygomaticomaxillary complex. Several techniques have been combined with orthognathic surgery to enhance the volume and definition of this region, such as zygomatic osteotomy. Two female patients, aged 22 (Case A) and 29 (Case B), with class II (Case A) and III (Case B) malocclusion, respectively, complained about facial aesthetics due to a long face and lack of malar projection. Le Fort I osteotomy to advance the maxilla, bilateral mandibular ramus osteotomy to set back the mandible, mentoplasty for advancement and width reduction, and zygomatic osteotomy were erformed. The patients underwent computed tomography scans, and superimposed images indicated that a volumetric increase in the malar region was achieved through the anterolateral movement of the zygomatic body. Improvements were noted in the paranasal region (Case A), better alignment of the upper and lower lips, the chin region, and an improved chin-neckline ratio (in both cases). The patients were satisfied with the results and are under follow-up. In summary, zygomatic osteotomy combined with orthognathic surgery proved to be a predictable and safe strategy for increasing malar prominence, with satisfactory aesthetic and functional outcomes.

Patients with anteroposterior maxillary deficiency and mandibular prognathism associated with a long-face pattern exhibit reduced anterior and lateral projection of the zygomaticomaxillary complex. Several techniques have been combined with orthognathic surgery to enhance the volume and definition of this region, such as zygomatic osteotomy. Two female patients, aged 22 (Case A) and 29 (Case B), with class II (Case A) and III (Case B) malocclusion, respectively, complained about facial aesthetics due to a long face and lack of malar projection. Le Fort I osteotomy to advance the maxilla, bilateral mandibular ramus osteotomy to set back the mandible, mentoplasty for advancement and width reduction, and zygomatic osteotomy were erformed. The patients underwent computed tomography scans, and superimposed images indicated that a volumetric increase in the malar region was achieved through the anterolateral movement of the zygomatic body. Improvements were noted in the paranasal region (Case A), better alignment of the upper and lower lips, the chin region, and an improved chin-neckline ratio (in both cases). The patients were satisfied with the results and are under follow-up. In summary, zygomatic osteotomy combined with orthognathic surgery proved to be a predictable and safe strategy for increasing malar prominence, with satisfactory aesthetic and functional outcomes.