Objective: To examine differences in depressive and anxiety symptoms between boarding and non boarding high school students and their associations with physical activity (PA) patterns, so as to provide evidence to inform adolescent mental health promotion. Methods: From October to December 2024, a convenience sample of 11 782 students aged 15-18 years was recruited from 36 schools in Nanchang, Ganzhou, and Shangrao of Jiangxi Province. Depressive and anxiety symptoms and PA were assessed using the Patient Health Questionnaire-9 (PHQ-9), Generalized Anxiety Disorder-7 (GAD-7), and International Physical Activity Questionary Short Form (IPAQ-SF). Logistic regression model was used to examine associations between PA patterns, depressive and anxiety symptoms among boarding and non boarding students. Results: The detection rates of depressive symptoms were 45.7% and 46.4% among boarding and non boarding students, respectively; for anxiety symptoms, the corresponding rates were 43.0% and 46.7%. Boarding and non boarding students differed significantly in smoking status, screen time, sleep duration, sedentary time, daily vegetable intake, and napping ( χ 2=16.74-664.17, all P <0.01). Across PA pattern groups, the detection rates of depressive and anxiety symptoms differed significantly between boarding and non boarding students ( χ 2 depression = 23.85 , χ 2 anxiety = 22.78, both P <0.01). Adjusted for confounding factors, Logistic regression analysis of high school students showed that compared with the not meeting PA recommendations, both the concentrated and regular PA pattern were associated with lower odds of depressive symptoms [ OR (95% CI )=0.83(0.70-0.98), 0.90(0.83-0.98)]; and the concentrated pattern was also associated with lower odds of anxiety symptoms [ OR (95% CI )=0.78(0.65-0.92)], and the association of anxiety symptoms in concentrated boarding students was consistent with that of the overall group [ OR (95% CI )=0.71(0.52-0.98)] (all P <0.05). Conclusions There is a correlation of different physical activity patterns with depressive and anxiety symptoms among boarding and non boarding high school students. Schools should ensure students engage in regular physical activity and work to increase overall activity volume.

Objective:To compare the effects of leukocyte-poor platelet-rich plasma (Lp-PRP) and leukocyte-rich platelet-rich plasma (Lr-PRP) on dorsal wound healing in rats.Methods:Thirty-six male Sprague-Dawley rats were randomly divided into Lp-PRP group, Lr-PRP group and control group, each containing twelve rats. Venous blood was drawn and the Lp-PRP and Lr-PRP were prepared separately using a centrifugal method. Circular full-thickness skin defect wounds (15 mm in diameter) were created on the backs of the rats in the three groups. The wounds were then treated with 100 μl Lp-PRP, Lr-PRP and saline, respectively. At 7 and 14 days post-operation, the wounds were photographed, and Image J software was used to calculate the wound area rate (postoperative wound area/wound area at modeling time × 100%). At 14 days post-operation, the total neo-epithelium length and collagen deposition rate of the wounds were evaluated using HE and Masson staining, respectively. At 7 days post-operation, the relative expression of vascular endothelial growth factor (VEGF) in the wounds was detected by Western blotting, and the number of CD31 positive microvessels in the wounds was examined by immunohistochemistry. Statistical analysis was performed using SPSS 28.0. One-way analysis of variance (ANOVA) was used to compare the three groups, and Tukey’s test was used for pairwise comparisons. A significance level of P<0.05 was considered statistically significant. Results:Blood analysis revealed that the platelet concentrations in the prepared Lp-PRP and Lr-PRP were 4.1 times and 4.5 times that of whole blood, respectively ( P<0.01), with no significant difference between the two PRPs ( P>0.05). The leukocyte concentration in Lp-PRP was undetectable, while in Lr-PRP, it was 3.5 times that of whole blood ( P<0.01). The wound area rate at 7 and 14 days post-operation, the total neo-epithelium length and collagen deposition rate at 14 days post-operation, as well as the relative expression of VEGF and the number of CD31-positive microvessels at 7 days post-operation in the Lp-PRP and Lr-PRP groups were superior to those in the control group (all P<0.01). However, there was no significant difference between the two PRP groups (all P>0.05). Conclusion:Both Lp-PRP and Lr-PRP promote dorsal wound healing in rats by enhancing re-epithelialization, collagen deposition, and angiogenesis. The impacts of Lp-PRP and Lr-PRP on promoting wound healing are comparable and not influenced by the presence of leukocytes in PRPs.

Objective:To compare the effects of leukocyte-poor platelet-rich plasma (Lp-PRP) and leukocyte-rich platelet-rich plasma (Lr-PRP) on dorsal wound healing in rats.Methods:Thirty-six male Sprague-Dawley rats were randomly divided into Lp-PRP group, Lr-PRP group and control group, each containing twelve rats. Venous blood was drawn and the Lp-PRP and Lr-PRP were prepared separately using a centrifugal method. Circular full-thickness skin defect wounds (15 mm in diameter) were created on the backs of the rats in the three groups. The wounds were then treated with 100 μl Lp-PRP, Lr-PRP and saline, respectively. At 7 and 14 days post-operation, the wounds were photographed, and Image J software was used to calculate the wound area rate (postoperative wound area/wound area at modeling time × 100%). At 14 days post-operation, the total neo-epithelium length and collagen deposition rate of the wounds were evaluated using HE and Masson staining, respectively. At 7 days post-operation, the relative expression of vascular endothelial growth factor (VEGF) in the wounds was detected by Western blotting, and the number of CD31 positive microvessels in the wounds was examined by immunohistochemistry. Statistical analysis was performed using SPSS 28.0. One-way analysis of variance (ANOVA) was used to compare the three groups, and Tukey’s test was used for pairwise comparisons. A significance level of P<0.05 was considered statistically significant. Results:Blood analysis revealed that the platelet concentrations in the prepared Lp-PRP and Lr-PRP were 4.1 times and 4.5 times that of whole blood, respectively ( P<0.01), with no significant difference between the two PRPs ( P>0.05). The leukocyte concentration in Lp-PRP was undetectable, while in Lr-PRP, it was 3.5 times that of whole blood ( P<0.01). The wound area rate at 7 and 14 days post-operation, the total neo-epithelium length and collagen deposition rate at 14 days post-operation, as well as the relative expression of VEGF and the number of CD31-positive microvessels at 7 days post-operation in the Lp-PRP and Lr-PRP groups were superior to those in the control group (all P<0.01). However, there was no significant difference between the two PRP groups (all P>0.05). Conclusion:Both Lp-PRP and Lr-PRP promote dorsal wound healing in rats by enhancing re-epithelialization, collagen deposition, and angiogenesis. The impacts of Lp-PRP and Lr-PRP on promoting wound healing are comparable and not influenced by the presence of leukocytes in PRPs.

Objective To evaluate the impact of optimizing the stroke green channel on the efficiency of acute ischemic stroke management in a county hospital. Methods A retrospective analysis of the emergency stroke green channel treatment data from Sixian People’s Hospital from May 2020 to April 2021 (before optimization of the green channel) and from May 2021 to April 2022 (after optimization of the green channel) was conducted. The rates of intravenous thrombolysis (IVT) and mechanical thrombectomy (MT) patients, as well as door-to-needle time (DNT), door-to-puncture time (DPT), and the modified Rankin scale (mRS) scores of patients three months post-treatment before and after the optimization of the stroke green channel were compared. Results Within one year before and after optimization of the green channel, the number of acute visits for ischemic stroke was 3 143 and 2 623, respectively. Before optimization, 84 and 51 underwent IVT and MT, respectively. After optimization of the green channel, the ratios of patients underwent IVT (n＝215) and MT (n＝103) significantly increased, and both DNT and DPT were significantly shortened (P＜0.000 1); the proportion of MT patients with an mRS score of 0-2 at 3 months post-discharge significantly increased (46/99 vs 13/46, P＝0.038). Conclusion After optimizing the green channel at Sixian People’s Hospital, the efficiency of stroke treatment has significantly improved, and the patients’ prognosis improved.

【Objective】 To explore the relationship between the storage time of leukodepleted red blood cells and transfusion adverse reactions by analyzing the occurrence of transfusion adverse reactions of patients after leukodepleted red blood cells transfusion from four hospitals. 【Methods】 By using the electronic medical record management system, the collection and transfusion dates of leukodepleted red blood cells from four hospitals in Enshi Prefecture from 2018 to 2022, as well as the information on transfusion adverse reactions, were retrieved. 【Results】 From 2018 to 2022, a total of 697 61 bags of leukodepleted red blood cells were transfused in four hospitals, resulting in 166 cases of transfusion adverse reactions, among which 93 were allergic reactions, 63 were non hemolytic febrile reactions, and 10 were others, with a total incidence rate of transfusion adverse reactions at 0.24%. The average storage time of leukodepleted red blood cells with and without transfusion adverse reactions was (20.25±6.31) and (19.88±5.50) days, respectively. With a storage time of 7 days as the threshold, the incidence of transfusion adverse reactions was the lowest for a storage time of 15~21 days. The incidence of transfusion adverse reactions of leukodepleted red blood cells in two groups (with storage days ≤21 days and >21 days) was not statistically significant(P>0.05). 【Conclusion】 Allergic reactions were the main type of transfusion adverse reaction caused by leukodepleted red blood cells, and the incidence of transfusion adverse reactions decreased and then increased with the prolongation of the storage time of leukodepleted red blood cells. There was no significant difference in the incidence of transfusion adverse reactions with leukodepleted red blood cells stored for ≤ 21 days and >21 days.

Antiviral Agents/pharmacology* ; COVID-19 ; Coronavirus 3C Proteases ; Humans ; Lactams ; Leucine ; Molecular Docking Simulation ; Molecular Dynamics Simulation ; Nitriles ; Proline ; Protease Inhibitors/pharmacology* ; SARS-CoV-2

Objective:To analyze the changes in the transcriptome of mouse fibroblasts after exposure to extremely low frequency electromagnetic fields (ELF-EMFs) using next-generation sequencing technology, and to screen out related pathways and genes that might be involved in the regulation of fibroblast growth by ELF-EMFs.Methods:The mouse NIH/3T3 cells were divided into the radiation group and the normal control group.The cells in the radiation group were placed in a 0.2 mT, 50 Hz electromagnetic radiation system, and the cells in the normal control group was placed in the same coil system under the same conditions without power.After 24-hour culture in a cell incubator, RNA was extracted.The next-generation high-throughput sequencing technology was used to sequence the transcriptomes of the two groups, and perform gene function annotation and signal pathway database analysis on the selected differential genes.Some highly expressed genes were screened out and verified by real-time fluorescent quantitative PCR.Results:A total of 17 980 genes were identified in the transcriptome sequencing, and there were 140 significantly differentially expressed genes (DEGs), of which 120 were up-regulated and 20 were down-regulated.DEGs were enriched in enzyme catalytic activity, cell metabolism process, biological regulation, biosynthesis and so on.According to the Kyoto Encyclopedia of Genes and Genomes analysis, the DEGs mainly involved 55 pathways, among which the most enriched 10 pathways were aminoacyl-tRNA biosynthesis, platelet activation, neurotrophin signaling pathway, renin-angiotensin system, etc., closely related to cell biosynthesis.The DEGs that might be involved in the post-irradiation stress of cells were further screened out, including mitogen activated protein kinase 12 ( MAPK12), neurotrophic tyrosine kinase receptor type 3 ( NTRK3), angiotensin Ⅱ receptor type 2 ( AGTR2), vascular endothelial growth factor ( VEGF), etc.Real-time fluorescent quantitative PCR results showed that the relative expression levels of MAPK12, NTRK3, AGTR2, VEGF mRNA in the radiation group were 2.389±0.003, 2.481±0.350, 2.354±0.081, 1.559±0.110, respectively, which were significantly higher than 1.011±0.190, 1.011±0.180, 1.007±0.150, 1.008±0.153, respectively in the normal control group ( t=12.540, 6.309, 13.710, 3.078; all at P<0.05). Conclusions:After the mouse fibroblasts were interfered with ELF-EMFs, the expression levels of MAPK12, NTRK3, AGTR2, VEGF and other genes are significantly up-regulated, which mainly involve neurotrophin signaling pathway, renin-angiotensin system and other pathways.These genes and pathways may be the main way that ELF-EMFs affect fibroblasts.

Since the first description by Hyakusoku in 1991, perforator propeller flaps have become one of the most commonly used method for defect reconstruction across the entire human body. We briefly reviewed the history, definition, nomenclature, and classification of perforator propeller flaps, and the corresponding surgical techniques were introduced in detail as well by reviewing our clinical cases. The clinical applications of the perforator propeller flaps were reviewed thoroughly by describing its use in different anatomic regions including the head and neck, trunk, extremities, buttock, and perineum respectively. Both the advantages and drawbacks of the flaps, common complications, and its prevention were also analyzed. Problems that we often encountered in the application of perforator propeller flaps and its current research status were analyzed and summarized. Although the perforator propeller flaps have been increasingly widely used, there still exist some important issues that deserve more concern and need to be solved, including precise perforator localization, accurate definition of perforators, correlations between the rotation angle of the flap, vascular patency and pedicle length. Though with the aforementioned problems, we believe that, like the tissue expansion and microsurgical flap transfer technique, the perforator propeller flap technique will ultimately become a conventional and important method for defect and organ reconstruction.

Since the first description by Hyakusoku in 1991, perforator propeller flaps have become one of the most commonly used method for defect reconstruction across the entire human body. We briefly reviewed the history, definition, nomenclature, and classification of perforator propeller flaps, and the corresponding surgical techniques were introduced in detail as well by reviewing our clinical cases. The clinical applications of the perforator propeller flaps were reviewed thoroughly by describing its use in different anatomic regions including the head and neck, trunk, extremities, buttock, and perineum respectively. Both the advantages and drawbacks of the flaps, common complications, and its prevention were also analyzed. Problems that we often encountered in the application of perforator propeller flaps and its current research status were analyzed and summarized. Although the perforator propeller flaps have been increasingly widely used, there still exist some important issues that deserve more concern and need to be solved, including precise perforator localization, accurate definition of perforators, correlations between the rotation angle of the flap, vascular patency and pedicle length. Though with the aforementioned problems, we believe that, like the tissue expansion and microsurgical flap transfer technique, the perforator propeller flap technique will ultimately become a conventional and important method for defect and organ reconstruction.

A large number of innovative and new technologies and method in the field of perforator flap were proposed with the hard work of many experts and surgeons. This paper briefly reviewed the following items including the discovery of the perforator flap and its enlightenment to us, three-dimensional visualized anatomy, angiosome and perforasome theories, pre-operative perforator detection techniques, techniques for evaluation of the flap vascular perfusion, the concept of freestyle perforator flap, chimeric flap, flow-through flap technique, new designs of pedicle perforator flap, particularly the propeller and keystone flaps, supramicrosurgery, superthin flap, microdissected thin perforator flap, and microvascular breast reconstruction and lymph node transfer for postmastectomy lymphedema patients. These new technologies and method have greatly improved our understanding of flap surgery and promote the development of the reconstructive surgery. The traditional research focused on investigating the anatomic features of a single flap and strategies for the reconstruction of different defects and organs. Nowadays, the treatment scope of reconstructive surgery is more extensive and has developed to repair various refractory wounds following trauma and oncological resection, nerve injuries, and to reconstruct defect and organ by using the vascularized composite allotransplantation. Researches on the upper limb lymphedema after breast cancer surgery have been highly valued recently. It can be expected that the direction of reconstructive surgery would be transformed from the improvement of theraputic modalities to concentrating on the treatment of diseases, and this undoubtedly conforms to the essence of medicine. The research of perforator flaps will be directed to more precise, minimally invasive, and individualized according to the requirements of evidence-based medicine.