Objective: To investigate the status of condom use and its influencing factors among men who have sex with men (MSM), so as to provide a basis for improving condom utilization rates and AIDS prevention and control in this population. Methods: From May to October 2024, a snowball sampling method was employed to recruit MSM in Songjiang District, Shanghai Municipality. Self-administered questionnaires were used to collect data on demographic characteristics, AIDS-related knowledge, sexual behaviors, pre-exposure prophylaxis (PrEP) and post-exposure prophylaxis (PEP), and condom use in the past six months. Multivariable logistic regression model was used to analyze the influencing factors for consistent condom use. Results: A total of 921 MSM were surveyed, with a median age of 29.00 (interquartile range, 9.00) years. Among them, 697 (75.68%) were aware of AIDS-related knowledge, 826 (89.69%) expressed willingness to use PrEP, and 835 (90.66%) were willing to use PEP. Additionally, 787 (85.45%) MSM reported their age at first homosexual intercourse as ≥18 years, while 519 (56.35%) reported consistent condom use in the past six months. Multivariable logistic regression analysis revealed that MSM who were aware of AIDS-related knowledge (OR=0.582, 95% CI: 0.423-0.801), willing to use PrEP (OR =0.611, 95% CI: 0.385-0.969), and whose age at first homosexual intercourse was <18 years (OR=0.480, 95% CI: 0.330-0.700) were less likely to consistent use condoms. Conclusion The proportion of consistent condom use among the MSM remains relatively low, which is primarily associated with AIDS-related knowledge, willingness to use PrEP, and the age at first homosexual intercourse.

Objective To establish a risk prediction model for cerebral edema after mechanical thrombectomy in acute cerebral infarction (ACI) based on multimodal computed tomography (CT) parameters. Methods A total of 220 ACI patients who underwent mechanical thrombectomy at The People’s Hospital of Bozhou between January 2022 and June 2025 were enrolled. All patients underwent preoperative conventional non-contrast CT, CT perfusion imaging, and multiphase CT angiography. Based on the presence or absence of cerebral edema at 72 hours postoperatively, patients were divided into cerebral edema and non-cerebral edema groups. General data and multimodal CT parameters were compared between the two groups. Multivariable logistic regression model was used to analyze the influencing factors of postoperative cerebral edema. A nomogram prediction model was constructed based on the multivariable analysis results, and the efficacy of the model was validated. Results The incidence of postoperative cerebral edema was 23.64% (52/220). Multivariable logistic regression analysis showed that time from onset to recanalization (OR=1.081, 95%CI=1.017-1.149), cerebral blood flow (OR=0.903, 95%CI=0.828-0.984), time to peak (OR=1.171, 95%CI=1.041-1.317), mean transit time (OR=2.815, 95%CI=1.823-6.178), and collateral circulation score (OR=0.960, 95%CI=0.930-0.990) were influencing factors for cerebral edema after mechanical thrombectomy in ACI patients (P<0.05). A nomogram model was constructed based on these factors. The calibration curve indicated good model fit, and the decision curve demonstrated net benefit. The area under the receiver operating characteristic curve for predicting cerebral edema after mechanical thrombectomy in patients with ACI was 0.961, with a sensitivity of 88.46% and a specificity of 91.07%. The Hosmer–Lemeshow goodness-of-fit test showed good model fit (χ²=1.766, P=0.962). Conclusion A risk prediction model for cerebral edema after mechanical thrombectomy in ACI patients was constructed using multimodal CT parameters. The model demonstrated high sensitivity and specificity.

Objective To establish a risk prediction model for cerebral edema after mechanical thrombectomy in acute cerebral infarction (ACI) based on multimodal computed tomography (CT) parameters. Methods A total of 220 ACI patients who underwent mechanical thrombectomy at The People’s Hospital of Bozhou between January 2022 and June 2025 were enrolled. All patients underwent preoperative conventional non-contrast CT, CT perfusion imaging, and multiphase CT angiography. Based on the presence or absence of cerebral edema at 72 hours postoperatively, patients were divided into cerebral edema and non-cerebral edema groups. General data and multimodal CT parameters were compared between the two groups. Multivariable logistic regression model was used to analyze the influencing factors of postoperative cerebral edema. A nomogram prediction model was constructed based on the multivariable analysis results, and the efficacy of the model was validated. Results The incidence of postoperative cerebral edema was 23.64% (52/220). Multivariable logistic regression analysis showed that time from onset to recanalization (OR=1.081, 95%CI=1.017-1.149), cerebral blood flow (OR=0.903, 95%CI=0.828-0.984), time to peak (OR=1.171, 95%CI=1.041-1.317), mean transit time (OR=2.815, 95%CI=1.823-6.178), and collateral circulation score (OR=0.960, 95%CI=0.930-0.990) were influencing factors for cerebral edema after mechanical thrombectomy in ACI patients (P<0.05). A nomogram model was constructed based on these factors. The calibration curve indicated good model fit, and the decision curve demonstrated net benefit. The area under the receiver operating characteristic curve for predicting cerebral edema after mechanical thrombectomy in patients with ACI was 0.961, with a sensitivity of 88.46% and a specificity of 91.07%. The Hosmer–Lemeshow goodness-of-fit test showed good model fit (χ²=1.766, P=0.962). Conclusion A risk prediction model for cerebral edema after mechanical thrombectomy in ACI patients was constructed using multimodal CT parameters. The model demonstrated high sensitivity and specificity.

Objective To establish a risk prediction model for cerebral edema after mechanical thrombectomy in acute cerebral infarction (ACI) based on multimodal computed tomography (CT) parameters. Methods A total of 220 ACI patients who underwent mechanical thrombectomy at The People’s Hospital of Bozhou between January 2022 and June 2025 were enrolled. All patients underwent preoperative conventional non-contrast CT, CT perfusion imaging, and multiphase CT angiography. Based on the presence or absence of cerebral edema at 72 hours postoperatively, patients were divided into cerebral edema and non-cerebral edema groups. General data and multimodal CT parameters were compared between the two groups. Multivariable logistic regression model was used to analyze the influencing factors of postoperative cerebral edema. A nomogram prediction model was constructed based on the multivariable analysis results, and the efficacy of the model was validated. Results The incidence of postoperative cerebral edema was 23.64% (52/220). Multivariable logistic regression analysis showed that time from onset to recanalization (OR=1.081, 95%CI=1.017-1.149), cerebral blood flow (OR=0.903, 95%CI=0.828-0.984), time to peak (OR=1.171, 95%CI=1.041-1.317), mean transit time (OR=2.815, 95%CI=1.823-6.178), and collateral circulation score (OR=0.960, 95%CI=0.930-0.990) were influencing factors for cerebral edema after mechanical thrombectomy in ACI patients (P<0.05). A nomogram model was constructed based on these factors. The calibration curve indicated good model fit, and the decision curve demonstrated net benefit. The area under the receiver operating characteristic curve for predicting cerebral edema after mechanical thrombectomy in patients with ACI was 0.961, with a sensitivity of 88.46% and a specificity of 91.07%. The Hosmer–Lemeshow goodness-of-fit test showed good model fit (χ²=1.766, P=0.962). Conclusion A risk prediction model for cerebral edema after mechanical thrombectomy in ACI patients was constructed using multimodal CT parameters. The model demonstrated high sensitivity and specificity.

Objective: To investigate whether zerumbone ( ZER) has the effect of preventing cisplatin ( Cis) -induced acute kidney injury (Cis-AKI) . Methods: The MTT method was used to detect the effect of different concentrations of ZER on the cell viability of Cis-AKI. The in vivo and in vitro models of Cis-AKI mice were estab- lished by dividing into control group , separate administration group , model group , and dose group. Western blot and immunofluorescence experiments were used to detect the expression changes of kidney injury marker-1 ( KIM- 1) , phosphorylated NF-κB p65 ( P-p65 ) , Cleaved casepase3 , receptor interacting protein kinase 1 ( RIPK1) , RIPK3 , and tumor necrosis factor-α (TNF-α) . Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the mRNA expression of KIM-1 , TNF-α , interleukin-6 ( IL-6) , and monocyte chemoattractant protein-1(MCP-1) . Periodic acid-Schiff (PAS) staining confirmed the therapeutic effect of ZER on Cis-AKI. RNA-seq and cell thermal shift (CETSA) were used to explore possible target proteins. Results : MTT results showed that ZER could alleviate the decrease in cell viability of Cis-AKI ; in vivo and in vitro studies showed that compared with the model group , after treatment with ZER , its KIM-1 , P-p65 , Cleaved casepased3 , RIPK1 , RIPK3 , TNF -α expres- sion decreased significantly , and the mRNA expression of KIM-1 , TNF-α , IL-6 mRNA , and MCP-1 decreased. PAS staining showed that ZER had a therapeutic effect on Cis-AKI. RNA-seq and CETSA analysis showed that ZER might prevent and treat Cis-AKI by targeting the PIM1 protein. Conclusion ZER may alleviate Cis-AKI and im- prove inflammatory response and necroptosis by regulating PIM1 protein. ZER is expected to be a potential drug for the prevention and treatment of Cis-AKI.

Objective To analyze the nutrition status of primary and middle school students in Baodi district,Tianjin in 2021 and 2023,as to provide scientific basis for improving the nutritional status of students.Methods A stratified random cluster sampling method was adopted to select 12 schools in Baodi district,Tianjin.Students' nutrition status were assessed from September to November of every year in 2021 and 2023,including questionnaire survey,physical examination and biochemical indicators.Results Compared with the same gender and age students in 2021,the height of boys aged 7-13 and girls aged 7-12 will significantly increase in 2023,while the body mass of boys and girls aged 8 and 11 will significantly increase.Compared with 2021,the overweight and obesity rates among males have significantly decreased in 2023,while the emaciation rates among males and females have significantly increased.The anemia rate,serum ferritin and vitamin A,and vitamin D deficiency rate among students in 2023 were 12.25％,3.77％,15.70％,and 50.31％,respectively,with no statistically significant difference compared to 2021(P＞0.05).The awareness rate of nutrition knowledge and the proportion of related behaviors among students in 2023 have significantly increased compared to 2021.Conclusion Compared with 2021,the overweight and obesity rates among primary and secondary school students will decrease in 2023,while anemia,serum ferritin,vitamin A,and vitamin D deficiencies will not improve.Targeted intervention activities are urgently needed.

Objective Through visual analysis of literatures related to the treatment of chronic glomerulonephritis(CGN),the research hotspots and trends in this field were discussed.Methods Relevant literatures from CNKI,Wanfang Data Knowledge Service Platform,VIP,SinoMed,PubMed from 2010 to 2024 were retrieved,and analyzed using CiteSpace 6.2.R4 software.Results A total of 8887 articles in Chinese and 117 articles in English were included.The countries,institutions and authors who published most were China,the Affiliated Hospital for Liaoning University of Traditional Chinese Medicine and Wang Yiping.The research hotspots mainly focued on therapeutic drugs and clinical efficacy,and the research trend tended to be the treatment mechanism.Conclusion To further strengthen collaboration among different countries,institutions and authors,and to delve deeper into the mechanistic studies of CGN,will effectively promote the research progress in this field.

ObjectiveTo investigate the effects and potential mechanisms of morning and evening fasting on postprandial lipid responses, a post hoc analysis based on a crossover randomized controlled trial was conducted to assess the effects of different fasting strategies on postprandial lipid metabolism in community residents in Shanghai. MethodsA total of 23 participants took part in a randomized crossover trial involving two intervention days: morning fasting and evening fasting, with a washout period of 6 days between intervention days. Two-way analysis of variance was used to test the differences in total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and the relative expression of circadian clock genes before and after the next meal under fasting. Wilcoxon rank sum tests were used to analyze the different metabolites between the two groups. Principal component analysis and Orthogonal partial least squares-discriminant analysis were conducted to evaluate the ability of metabolites to differentiate between morning fasting and evening fasting and identify the important differential metabolites. After adjusting for age, sex, and BMI, a partial correlation analysis was performed to identify metabolites associated with plasma lipids. In addition, important metabolites associated with plasma lipids were computed by pathway enrichment analysis. ResultsAfter evening fasting intervention, fasting TG level [(0.37±0.29) vs (0.27±0.18)] mmol·L^-1, fasting and postprandial change values in TC [(2.74±0.47) vs (2.51±0.27)] mmol·L^-1 and LDL-C [(1.32±0.38) vs (0.99±0.27)] mmol·L^-1 were significantly lower than those after morning fasting (P<0.05). While, change values of fasting LDL-C [(0.89±0.37) vs (1.14±0.37)] mmol·L^-1 and TG [(1.14±0.19) vs (1.28±0.17)] mmol·L^-1 were significantly higher than those after morning fasting intervention (P<0.05). After fasting intervention, the relative expression of AMPK, CRY1, CLOCK, MTNR1B, AANAT, and ASMT was correlated with the amount of plasma lipid changes (P<0.05). Specifically, CLOCK and AANAT were upregulated following evening fasting and downregulated after morning fasting. Among the 217 important differential metabolites, 111 were correlated with plasma lipids, and which were primarily enriched in the cysteine and methionine metabolism pathways (P<0.05). ConclusionCompared to morning fasting, evening fasting was more effective in improving postprandial lipid responses, indicating that an evening fasting window during intermittent fasting could be conducive to cardiovascular disease prevention in adults. Meanwhile, it is suggested that morning and evening fasting may affect lipid responses through circadian rhythm oscillations and the cysteine and methionine metabolism pathways.

Objective:To compare the efficacy of new nasopharyngeal airway and laryngeal mask airway for airway management in the patients undergoing non-intubated video-assisted thoracoscopic surgery (NIVATS).Methods:In this randomised, controlled, non-inferiority trial, 60 American Society of Anesthesiologists Physical Status classification I or Ⅱ patients of both sexes, aged 18-79 yr, scheduled for elective NIVATS from December 2021 to December 2023 at Jining No.1 People′s Hospital, were divided into 2 groups ( n=30 each) using a computer-generated random code in a 1∶1 ratio: new type nasopharyngeal airway group (group N) and laryngeal mask airway group (group L). After anesthesia induction, a new nasopharyngeal airway was inserted in group N, and a laryngeal mask airway was inserted in group L. Spontaneous ventilation was maintained during the NIVATS. Ultrasound-guided serratus anterior plane block was performed on the affected side before anesthesia induction. Anesthesia was maintained with propofol and remifentanil. The primary outcome measure was the rate of intraoperative airway intervention, the airway interventions included repositioning of the airway tools, manual assisted ventilation, jaw-thrust maneuver, and conversion to endotracheal intubation. The secondary outcome measures included the first-attempt success rate of airway device placement, time for establishing a patent airway, the minimum value of SpO 2, the maximum value of P ETCO 2, and incidence of complications such as postoperative sore throat. Results:The rate of intraoperative airway intervention was 27% in group L and 47% in group N ( χ2=2.58, P=0.108). The difference in the rate of intraoperative airway intervention between the two groups was 0.20 (95% confidence interval 0.15-0.25), with a 95% confidence interval upper limit higher than the non-inferiority boundary (10%), indicating that this non-inferiority hypothesis was not established. In comparison to group L, the rate of intraoperative jaw-thrust maneuver intervention was significantly increased, the time to establish a patent airway was shortened, and the incidence of postoperative sore throat was decreased in group N ( P<0.05). Conclusions:Compared with the laryngeal mask airway, the new nasopharyngeal airway can reduce the development of postoperative throat pain, however, it is less effective in maintaining a patent airway. It requires careful consideration of risks and benefits when used for NIVATS.

Intestinal radiosensitivity makes radiation-induced intestinal injury (RIII) as a common complication among individuals exposed to accidental radiation and patients with abdominal or pelvic malignancies receiving radiotherapy. At present, there are no effective measures for the prevention and treatment of RIII. Developing novel prevention and treatment strategies is of great significance for improving the prognosis and quality of life for both individuals exposed to accidental radiation and cancer patients. Lactobacillus, a Gram-positive bacterium, is widely observed in humans and animals. It exhibits notable probiotic functions, including maintaining gut microbiota homeostasis, preserving intestinal barrier, and modulating immune responses. Substantial studies have demonstrated that Lactobacillus has remarkable protective and therapeutic effects against radiation-induced injury, specifically significantly alleviating RIII. These probiotic functions of Lactobacillus make it have high priority to become the chassis of genetically engineered strains. However, multiple factors influence the construction of a genetically engineered strain, limiting the application of Lactobacillus as a chassis. This review focuses on the probiotic functions of Lactobacillus, highlighting advances in research on Lactobacillus and its engineered probiotics in radiation protection and treatment. Furthermore, it summarizes key factors influencing the construction of genetically engineered strains based on the Lactobacillus chassis. It aims to provide insights for developing genetically engineered strains with radioprotective and therapeutic effects using Lactobacillus as the chassis.