Objective:To study the expression and regulatory mechanisms of pyroptosis in pulpitis.Methods:The activation sta-tus of cell deathes in pulpitis was analyzed using GSE77459 and GSE92681 datasets.Subsequently,pulp tissue and gingival crevic-ular fluid samples were collected from the subjects with pulpitis(infected)and healthy(non-infected)(n=10)respectively.The levels of GSDMD,NLRP3,caspase-1 and apoptosis proteins were detected by RT-qPCR and Western blot;the levels of IL-1βand IL-18 were assessed by ELISA;and the levels of reactive oxygen species(ROS)and mitochondrial membrane potential were exam-ined by probe method.Results:The levels of pyroptosis in the samples of patients with pulpitis in both GSE77459 and GSE92681 datasets were significantly higher than those in the control group(P＜0.05).RT-qPCR and Western blot analyses revealed that compared to controls,the expression of GSDMD,NLRP3 and caspase-1 in the samples of pulpitis was significantly higher(P＜0.05);the levels of IL-1β,IL-18,ROS and mitochondrial membrane potential in pulpitis were significantly elevated;the levels of eleaved caspase-3,caspase-8 and caspase-9 were significantly increased.Conclusion:The elevated level of pyroptosis in pulpitis that promotes inflammation may be related to mitochondrial dysfunction.

Objective:To study the expression and regulatory mechanisms of pyroptosis in pulpitis.Methods:The activation sta-tus of cell deathes in pulpitis was analyzed using GSE77459 and GSE92681 datasets.Subsequently,pulp tissue and gingival crevic-ular fluid samples were collected from the subjects with pulpitis(infected)and healthy(non-infected)(n=10)respectively.The levels of GSDMD,NLRP3,caspase-1 and apoptosis proteins were detected by RT-qPCR and Western blot;the levels of IL-1βand IL-18 were assessed by ELISA;and the levels of reactive oxygen species(ROS)and mitochondrial membrane potential were exam-ined by probe method.Results:The levels of pyroptosis in the samples of patients with pulpitis in both GSE77459 and GSE92681 datasets were significantly higher than those in the control group(P＜0.05).RT-qPCR and Western blot analyses revealed that compared to controls,the expression of GSDMD,NLRP3 and caspase-1 in the samples of pulpitis was significantly higher(P＜0.05);the levels of IL-1β,IL-18,ROS and mitochondrial membrane potential in pulpitis were significantly elevated;the levels of eleaved caspase-3,caspase-8 and caspase-9 were significantly increased.Conclusion:The elevated level of pyroptosis in pulpitis that promotes inflammation may be related to mitochondrial dysfunction.