Objective To explore the application of the"learning by doing"learning theory and methods in the teaching of Obstetrics and Gynecology Nursing for undergraduate nursing students.Method By setting up an experimental group of 151 students and a control group of 152 students in the teaching of Obstetrics and Gynecology Nursing for undergraduate nursing students,practicing"learning by doing"and evaluating the teaching effect.Result The experimental group students held a positive attitude towards the implementation of"learning by doing"in the teaching of Obstetrics and Gynecology Nursing for undergraduate nursing students,with higher scores in both theoretical and skill exams than the control group(P＜0.05),and achieved good practical results.Conclusion By comparing the theoretical and skill exam scores of the experimental group and the control group,the experimental group had a higher average score than the control group(P＜0.05).The application of"learning by doing"in the teaching of Obstetrics and Gynecology Nursing for undergraduate nursing has improved students'self-learning ability and empowered their innovative and collaborative growth.