Objective To explore the positive distribution of antinuclear antibody(ANA)and antinuclear antibody spectrum(ANAs)specific antibody detection in patients with chronic kidney disease(CKD)and its clinical application.Methods Indirect immunofluorescence(IIF)and line immunoassay(LIA)were used to detect ANA and ANAs in the sera of 3 968 CKD patients.Results The positive rate of IIF-ANA was 14.34％,that of LIA-ANAs was 26.84％,and the coincidence rate of the two detection results was 74.65％.In IIF-ANA,the distribution of nuclear patterns showed that the highest proportion was the nuclear speckled type(38.87％),followed by the cytoplasmic speckled type(20.06％).In LIA-ANAs,anti-SSB antibody showed the highest positive rate(13.40％),followed by anti-SSA antibody(13.33％).Additionally,the positive rates of IIF-ANA and LIA-ANAs were 20.44％and 30.35％respectively in females,significantly higher than those of males at 9.98％and 24.33％(P＜0.05),and also showed a relatively high positive rate in patients under 20 years old at 46.15％and 38.46％(P＜0.05).Among patients with stage 5 CKD(CKD5),the positive detection rate of ANA was the highest at 15.30％,and the specific antibody indicators with relatively high detection rates in stages 1 to 5 mainly included anti-SSB antibody(average 5.12％)and anti-SSA antibody(average 5.09％).Conclusion The positive detection rates and consistent coincidence rates of autoantibodies in CKD patients,detected by IIF and LIA,are relatively high,especially in young women,middle-aged and elderly women,and patients who have progressed to stage 5.The positive rate of IIF-ANA increases with the progression of the disease.Therefore,routine detection of autoantibodies in patients is of great value and significance for the early identification of autoimmune diseases and can provide assistance for the treatment and prevention of CKD.

Objective To explore the positive distribution of antinuclear antibody(ANA)and antinuclear antibody spectrum(ANAs)specific antibody detection in patients with chronic kidney disease(CKD)and its clinical application.Methods Indirect immunofluorescence(IIF)and line immunoassay(LIA)were used to detect ANA and ANAs in the sera of 3 968 CKD patients.Results The positive rate of IIF-ANA was 14.34％,that of LIA-ANAs was 26.84％,and the coincidence rate of the two detection results was 74.65％.In IIF-ANA,the distribution of nuclear patterns showed that the highest proportion was the nuclear speckled type(38.87％),followed by the cytoplasmic speckled type(20.06％).In LIA-ANAs,anti-SSB antibody showed the highest positive rate(13.40％),followed by anti-SSA antibody(13.33％).Additionally,the positive rates of IIF-ANA and LIA-ANAs were 20.44％and 30.35％respectively in females,significantly higher than those of males at 9.98％and 24.33％(P＜0.05),and also showed a relatively high positive rate in patients under 20 years old at 46.15％and 38.46％(P＜0.05).Among patients with stage 5 CKD(CKD5),the positive detection rate of ANA was the highest at 15.30％,and the specific antibody indicators with relatively high detection rates in stages 1 to 5 mainly included anti-SSB antibody(average 5.12％)and anti-SSA antibody(average 5.09％).Conclusion The positive detection rates and consistent coincidence rates of autoantibodies in CKD patients,detected by IIF and LIA,are relatively high,especially in young women,middle-aged and elderly women,and patients who have progressed to stage 5.The positive rate of IIF-ANA increases with the progression of the disease.Therefore,routine detection of autoantibodies in patients is of great value and significance for the early identification of autoimmune diseases and can provide assistance for the treatment and prevention of CKD.