Objective To investigate the effects of sample transport and sorting system on the detection results of common clinical bio-chemical and immunological items carried out in our laboratory.Methods A total of 25 patients admitted to Zidong Hospital of Jiangsu Provincial Hospital of Traditional Chinese Medicine in June 2024 were included in this study,and four blood samples were collected from each patients using vacuum blood vessels with separation gel containing coagulant.These samples were transferred to the laboratory department through manual transport or pneumatic logistics transmission,and the manual sorting approach or intelligent blood vessel sorting system was used to encode the samples.The effects of different sample transportation methods and sorting methods on the detec-tion results of 46 clinical biochemical parameters,12 tumor markers and 5 thyroid hormone items,which were carried out in our labora-tory,were analyzed and compared.Results No significant change on hemolytic index(HI)was found through pneumatic tube system(PTS)and intelligent blood vessel sorting system(P＞0.05).The results of AST,CK-MB,α-HBDH,and LDH in clinical biochemical parameters following PTS were significantly different from those in artificial transport group(all P＜0.05).Both the results of Cyfra21-1 and NSE in immunological items in the samples after PTS transport were significantly different from those obtained by either manual transport or intelligent sorting system,with statistical significance(all P＜0.05).Conclusion PTS basically meets the requirements of clinical laboratories,but it can lead to the increase of AST,CK-MB,α-HBDH and LDH in clinical biochemistry,as well as Cyfra21-1 and NSE in immunology,which needs to be further improved,refined,and validated in order to meet the clinical requirements.

Objective To investigate the effects of sample transport and sorting system on the detection results of common clinical bio-chemical and immunological items carried out in our laboratory.Methods A total of 25 patients admitted to Zidong Hospital of Jiangsu Provincial Hospital of Traditional Chinese Medicine in June 2024 were included in this study,and four blood samples were collected from each patients using vacuum blood vessels with separation gel containing coagulant.These samples were transferred to the laboratory department through manual transport or pneumatic logistics transmission,and the manual sorting approach or intelligent blood vessel sorting system was used to encode the samples.The effects of different sample transportation methods and sorting methods on the detec-tion results of 46 clinical biochemical parameters,12 tumor markers and 5 thyroid hormone items,which were carried out in our labora-tory,were analyzed and compared.Results No significant change on hemolytic index(HI)was found through pneumatic tube system(PTS)and intelligent blood vessel sorting system(P＞0.05).The results of AST,CK-MB,α-HBDH,and LDH in clinical biochemical parameters following PTS were significantly different from those in artificial transport group(all P＜0.05).Both the results of Cyfra21-1 and NSE in immunological items in the samples after PTS transport were significantly different from those obtained by either manual transport or intelligent sorting system,with statistical significance(all P＜0.05).Conclusion PTS basically meets the requirements of clinical laboratories,but it can lead to the increase of AST,CK-MB,α-HBDH and LDH in clinical biochemistry,as well as Cyfra21-1 and NSE in immunology,which needs to be further improved,refined,and validated in order to meet the clinical requirements.

Objective To explore the correlation between cold-induced RNA binding-protein(CIRP)and NF-κB levels with cognitive dysfunction in elderly patients with ischemic cerebral small vessel disease.Methods 135 elderly patients with ischemic cerebral small vessel disease admitted to our hospital from March 2021 to June 2024 were selected as research subjects.They were divided into a cognitive normal group(n=48)and a cognitive impairment group(n=87)based on whether cognitive dysfunction occurred.Multiple linear regression was used to analyze the relationship between cognitive impairment and inflammatory indicators,while the dose-response relationship of CIRP and NF-κB with cognitive impairment were estimated using restricted cubic spline method.The cognitive impairment group was further divided into mild cognitive impairment group(n=30),moderate cognitive impairment group(n=32),and severe cognitive impairment group(n=25)based on the severity of the condition.Ordered regression analysis was used to investigate the impact of CIRP and NF-κB on patient's condition;ROC curve was used to evaluate the predictive value of CIRP and NF-κB for moderate to severe cognitive impairment,and explore the mediating role of inflammation related factors between CIRP/NF-κB and mini-mental state examination(MMSE)scores.Results TNF-α,IL-6,IL-1β,CIRP and NF-κB are all factors affecting cognitive impairment(P＜0.05).CIRP and NF-κB were significantly positively correlated with TNF-α,IL-4,IL-10,IL-1β,MCP-1,TGF-β1 and hs CRP(P＜0.05),and significantly negatively correlated with TGF-β1(P＜0.05).The correlation of CIRP and NF-κB levels with cognitive impairment showed a non-linear dose-response relationship(P＜0.05).There was still an independent correlation(P＜0.05)of TNF-α,IL-6,IL-1β,CIRP and NF-κB with the severity of cognitive impairment.High levels of CIRP and NF-κB showed a synergistic effect with high levels of TNF-α,IL-6 and IL-1 β.The AUC value of CIRP and NF-κB for predicting severe cognitive impairment were 0.745 and 0.792,respectively,with a combined prediction AUC of 0.840.TNF-α,IL-4,IL-10,IL-1β,MCP-1,TGF-β1 and hs CRP play a mediating regulatory role between MMSE score and CIRP/NF-κB.Conclusion CIRP and NF-κB participate in the regulation of inflammatory responses and are closely related to cognitive dysfunction and severity of elderly patients with ischemic cerebrovascular disease.Monitoring the changes of CIRP and NF-κB are beneficial for clinical evaluation of patients' cognitive function.

Objective To explore the correlation between cold-induced RNA binding-protein(CIRP)and NF-κB levels with cognitive dysfunction in elderly patients with ischemic cerebral small vessel disease.Methods 135 elderly patients with ischemic cerebral small vessel disease admitted to our hospital from March 2021 to June 2024 were selected as research subjects.They were divided into a cognitive normal group(n=48)and a cognitive impairment group(n=87)based on whether cognitive dysfunction occurred.Multiple linear regression was used to analyze the relationship between cognitive impairment and inflammatory indicators,while the dose-response relationship of CIRP and NF-κB with cognitive impairment were estimated using restricted cubic spline method.The cognitive impairment group was further divided into mild cognitive impairment group(n=30),moderate cognitive impairment group(n=32),and severe cognitive impairment group(n=25)based on the severity of the condition.Ordered regression analysis was used to investigate the impact of CIRP and NF-κB on patient's condition;ROC curve was used to evaluate the predictive value of CIRP and NF-κB for moderate to severe cognitive impairment,and explore the mediating role of inflammation related factors between CIRP/NF-κB and mini-mental state examination(MMSE)scores.Results TNF-α,IL-6,IL-1β,CIRP and NF-κB are all factors affecting cognitive impairment(P＜0.05).CIRP and NF-κB were significantly positively correlated with TNF-α,IL-4,IL-10,IL-1β,MCP-1,TGF-β1 and hs CRP(P＜0.05),and significantly negatively correlated with TGF-β1(P＜0.05).The correlation of CIRP and NF-κB levels with cognitive impairment showed a non-linear dose-response relationship(P＜0.05).There was still an independent correlation(P＜0.05)of TNF-α,IL-6,IL-1β,CIRP and NF-κB with the severity of cognitive impairment.High levels of CIRP and NF-κB showed a synergistic effect with high levels of TNF-α,IL-6 and IL-1 β.The AUC value of CIRP and NF-κB for predicting severe cognitive impairment were 0.745 and 0.792,respectively,with a combined prediction AUC of 0.840.TNF-α,IL-4,IL-10,IL-1β,MCP-1,TGF-β1 and hs CRP play a mediating regulatory role between MMSE score and CIRP/NF-κB.Conclusion CIRP and NF-κB participate in the regulation of inflammatory responses and are closely related to cognitive dysfunction and severity of elderly patients with ischemic cerebrovascular disease.Monitoring the changes of CIRP and NF-κB are beneficial for clinical evaluation of patients' cognitive function.

Objective:To investigate the effects of different feeding formulas on the feeding and growth and metabolism of premature infants in the early postnatal period.Methods:Eligible premature infants with the gestational age of ≤ 34 weeks hospitalized from March 2023 to March 2024 were selected as per inclusion criteria, excluding those with congenital metabolic diseases, severe congenital heart disease and developmental malformations of digestive tract. According to the feeding formulas within 2 weeks after birth, premature infants were divided into three groups, namely donor human milk (DHM) group, preterm formula (PF) group and extensively hydrolyzed formula (eHF) group. The characteristics of premature infants, perinatal condition, feeding formulas, milk intake on the 7th and 14th day, the time to the daily milk intake of 120ml/kg and 150ml/kg respectively, the time on parenteral nutrition, the length of hospitalization, feeding intolerance, cholestasis, extrauterine growth retardation and biochemical metabolic indexes at 7 days, 14 days and discharge were collected. The differences of feeding and biochemical metabolic parameters were compared across the three groups.Results:A total of 108 cases were enrolled ,of whom 39 were in DHM group, 37 in PF group and 32 in eHF group. There was no significant difference in gestational age, birth weight, head circumference and maternal complications across the three groups. The milk intake in the DHM group was (50.7±29.1) ml/(kg·d) on the 7th day, compared with (34.2±27.3) ml/(kg·d) in PF group ( P=0.031), and (103.1±36.7) ml/(kg·d) on the 14th day, compared with (73.9±39.2) ml/(kg·d) in the PF group. Compared with the PF group, the DHM group reached the daily milk intake of 120 ml/(kg·d) earlier [(18.5±10.4) days vs. (24.1±10.3) days, P=0.020], had shorter duration of parenteral nutrition [(17.9±10.9) days vs. (23.2±11.2) days, P=0.042], and lower incidence of feeding intolerance (28.2% vs. 48.6%). The length of hospitalization in DHM group was shorter than that in PF group [(33.8±15.5) days vs. (37.8±17.6) days], but there was no significant difference ( P>0.05). There was no significant difference between the DHM group and the eHF group in terms of the milk intake on the 7th and 14th day, the time to the daily milk intake of 120 ml/(kg·d), the time on parenteral nutrition, the length of hospitalization and feeding intolerance. At 1 and 2 weeks after birth, alkaline phosphatase in DHM group was higher than that in PF group and eHF group ( P<0.05), but there was no significant difference in biochemical nutritional metabolism parameters (hemoglobin, urea nitrogen, albumin, prealbumin, alkaline phosphatase and total bile acid) across the three groups at discharge( P>0.05). Conclusion:Early use of DHM in premature infants is better tolerated than PF and can help achieve complete enteral nutrition earlier and shorten the use of parenteral nutrition, while not affecting the growth and development of premature infants.