Objective:To investigate the efficacy of warm needle acupuncture combined with bone manipulation and Tuina treatment in the treatment of lumbar disc herniation (LDH) and its effects on patients' lumbar function and quality of life. Methods:Fifty patients with LDH admitted to Wenzhou Traditional Chinese Medicine Hospital from December 2022 to December 2023 were included in this study. The patients were randomly divided into an observation group ( n = 25) and a control group ( n = 25) using the random number table method. Patients in the control group received bone manipulation and Tuina treatment, while those in the observation group received warm needle acupuncture combined with bone manipulation and Tuina treatment. Both groups underwent a treatment course of 4 weeks. The therapeutic effects were compared between the two groups. Additionally, the Oswestry Disability Index score, Visual Analog Scale score, Japanese Orthopaedic Association score, and the General Quality of Life Inventory (GQOLI-74) score were compared between the two groups before and after treatment. Results:The total effective rate of treatment in the observation group was significantly higher than that in the control group [96.00% (24/25) vs. 68.00% (17/25), χ2 = 4.88, P < 0.05]. After treatment, the Oswestry Disability Index score and Visual Analog Scale score in the observation group were (9.82 ± 1.76) and (1.72 ± 0.46), respectively, which were significantly lower than those in the control group [(13.97 ± 2.54), (2.89 ± 0.71), t = 6.71, 6.91, both P < 0.001]. In the observation group, the scores for limitations in daily activities, clinical symptoms, subjective symptoms, and the total score were (12.32 ± 2.17), (4.89 ± 0.72), (7.32 ± 1.18), and (24.53 ± 2.94), respectively. These scores were all significantly higher than those in the control group [(9.98 ± 1.64), (3.67 ± 0.58), (5.98 ± 0.76), (19.63 ± 2.87), t = 4.30, 6.60, 4.77, 5.96, all P < 0.001]. Additionally, the scores for social function, psychological function, and physical function in the observation group were (76.82 ± 6.08), (73.42 ± 6.92), and (78.28 ± 6.98), respectively. These scores were significantly higher than those in the control group [(63.32 ± 7.13), (60.09 ± 5.85), (65.21 ± 5.59), t = 7.20, 7.35, 7.31, all P < 0.001]. Conclusions:Warm needle acupuncture combined with bone manipulation and Tuina treatment is highly effective on LDH. The approach can improve patients' lumbar function and quality of life.

With the advancement of science and technology and the increasing care needs of critically ill patients, critical care nursing robots have developed rapidly. This paper provides an overview of the current applications of nursing robots in intensive care settings, including assessment and monitoring, routine care, rehabilitative care, emotional assistance, remote care, and multifunctional integration. Furthermore, the challenges associated with the implementation of critical care nursing robots and future development directions are discussed, aiming to provide a reference for the optimization and practical application of such technologies.

Objective:To investigate the efficacy of warm needle acupuncture combined with bone manipulation and Tuina treatment in the treatment of lumbar disc herniation (LDH) and its effects on patients' lumbar function and quality of life. Methods:Fifty patients with LDH admitted to Wenzhou Traditional Chinese Medicine Hospital from December 2022 to December 2023 were included in this study. The patients were randomly divided into an observation group ( n = 25) and a control group ( n = 25) using the random number table method. Patients in the control group received bone manipulation and Tuina treatment, while those in the observation group received warm needle acupuncture combined with bone manipulation and Tuina treatment. Both groups underwent a treatment course of 4 weeks. The therapeutic effects were compared between the two groups. Additionally, the Oswestry Disability Index score, Visual Analog Scale score, Japanese Orthopaedic Association score, and the General Quality of Life Inventory (GQOLI-74) score were compared between the two groups before and after treatment. Results:The total effective rate of treatment in the observation group was significantly higher than that in the control group [96.00% (24/25) vs. 68.00% (17/25), χ2 = 4.88, P < 0.05]. After treatment, the Oswestry Disability Index score and Visual Analog Scale score in the observation group were (9.82 ± 1.76) and (1.72 ± 0.46), respectively, which were significantly lower than those in the control group [(13.97 ± 2.54), (2.89 ± 0.71), t = 6.71, 6.91, both P < 0.001]. In the observation group, the scores for limitations in daily activities, clinical symptoms, subjective symptoms, and the total score were (12.32 ± 2.17), (4.89 ± 0.72), (7.32 ± 1.18), and (24.53 ± 2.94), respectively. These scores were all significantly higher than those in the control group [(9.98 ± 1.64), (3.67 ± 0.58), (5.98 ± 0.76), (19.63 ± 2.87), t = 4.30, 6.60, 4.77, 5.96, all P < 0.001]. Additionally, the scores for social function, psychological function, and physical function in the observation group were (76.82 ± 6.08), (73.42 ± 6.92), and (78.28 ± 6.98), respectively. These scores were significantly higher than those in the control group [(63.32 ± 7.13), (60.09 ± 5.85), (65.21 ± 5.59), t = 7.20, 7.35, 7.31, all P < 0.001]. Conclusions:Warm needle acupuncture combined with bone manipulation and Tuina treatment is highly effective on LDH. The approach can improve patients' lumbar function and quality of life.

With the advancement of science and technology and the increasing care needs of critically ill patients, critical care nursing robots have developed rapidly. This paper provides an overview of the current applications of nursing robots in intensive care settings, including assessment and monitoring, routine care, rehabilitative care, emotional assistance, remote care, and multifunctional integration. Furthermore, the challenges associated with the implementation of critical care nursing robots and future development directions are discussed, aiming to provide a reference for the optimization and practical application of such technologies.

Objective:To investigate the effects of stellate ganglion block(SGB)on serum inflammatory cytokines and the expression of toll-like receptor 4(TLR4),cysteine aspartate protease 3(caspase-3),and NF-κB(p65)pro-teins in rats with acute peritonitis(AP),with the goal of elucidating the mechanisms through which SGB modulates inflammatory responses.Methods:40 SPF grade male rats were randomly assigned to 4 groups:control group(Con-trol),acute peritonitis group(AP),acute peritonitis+right stellate ganglion block group(AP+SGB),and acute peri-tonitis+right stellate ganglion block+TLR4 inhibitor resatorvid group(AP+SGB+TAK-242),with 10 rats in each group.Acute peritonitis was induced in the AP,AP+SGB,and AP+SGB+TAK-242 groups via intraperitoneal injec-tion of 1 ml/100 g of 2％acetic acid solution.After establishing the model,blood samples were collected from the tail vein at 1 h,6 h,12 h,and 24 h respectively.Serum levels of interleukin-10(IL-10),tumor necrosis factor-alpha(TNF-α),interleukin-1 β(IL-1 β),and high mobility group protein B1(HMGB1)were measured using ELISA.After 24 hours,the peritoneal tissues were collected for analysis.Protein levels of TLR4,caspase-3,and NF-κB(p65)were quantified via Western Blot,while simultaneously,their mRNA expression levels were assessed using RT-qPCR.His-topathological alterations were evaluated by hematoxylin and eosin(HE)staining and then observed under optical microscopy.Results:At all time points,the AP,AP+SGB,and AP+SGB+TAK-242 groups showed significantly higher levels of IL-10,TNF-α,IL-1 β,and HMGB1 compared to the S group(P＜0.05).The AP+SGB group exhibi-ted significantly lower TNF-α,IL-1 β,and HMGB1 levels and higher IL-10 levels than the AP group at corresponding time points(P＜0.05).The AP+SGB+TLR4 group demonstrated further reductions in TNF-α,IL-1 β,and HMGB1 levels and additional increases in IL-10 levels relative to the AP+SGB group(P＜0.05).Both the AP+SGB and AP+SGB+TAK-242 groups showed significant decreases in TLR4,caspase-3,and NF-κB(p65)protein expression(P＜0.05).Similarly,TLR4 and NF-κB(p65)mRNA expression levels were markedly downregulated in these groups(P＜0.05).Conclusion:SGB can mitigate the inflammatory response in acute peritonitis rats by inhibiting the TLR4/NF-κB signaling pathway through the downregulation of TLR4 and NF-κB(p65).

Objective:To investigate the effects of stellate ganglion block(SGB)on serum inflammatory cytokines and the expression of toll-like receptor 4(TLR4),cysteine aspartate protease 3(caspase-3),and NF-κB(p65)pro-teins in rats with acute peritonitis(AP),with the goal of elucidating the mechanisms through which SGB modulates inflammatory responses.Methods:40 SPF grade male rats were randomly assigned to 4 groups:control group(Con-trol),acute peritonitis group(AP),acute peritonitis+right stellate ganglion block group(AP+SGB),and acute peri-tonitis+right stellate ganglion block+TLR4 inhibitor resatorvid group(AP+SGB+TAK-242),with 10 rats in each group.Acute peritonitis was induced in the AP,AP+SGB,and AP+SGB+TAK-242 groups via intraperitoneal injec-tion of 1 ml/100 g of 2％acetic acid solution.After establishing the model,blood samples were collected from the tail vein at 1 h,6 h,12 h,and 24 h respectively.Serum levels of interleukin-10(IL-10),tumor necrosis factor-alpha(TNF-α),interleukin-1 β(IL-1 β),and high mobility group protein B1(HMGB1)were measured using ELISA.After 24 hours,the peritoneal tissues were collected for analysis.Protein levels of TLR4,caspase-3,and NF-κB(p65)were quantified via Western Blot,while simultaneously,their mRNA expression levels were assessed using RT-qPCR.His-topathological alterations were evaluated by hematoxylin and eosin(HE)staining and then observed under optical microscopy.Results:At all time points,the AP,AP+SGB,and AP+SGB+TAK-242 groups showed significantly higher levels of IL-10,TNF-α,IL-1 β,and HMGB1 compared to the S group(P＜0.05).The AP+SGB group exhibi-ted significantly lower TNF-α,IL-1 β,and HMGB1 levels and higher IL-10 levels than the AP group at corresponding time points(P＜0.05).The AP+SGB+TLR4 group demonstrated further reductions in TNF-α,IL-1 β,and HMGB1 levels and additional increases in IL-10 levels relative to the AP+SGB group(P＜0.05).Both the AP+SGB and AP+SGB+TAK-242 groups showed significant decreases in TLR4,caspase-3,and NF-κB(p65)protein expression(P＜0.05).Similarly,TLR4 and NF-κB(p65)mRNA expression levels were markedly downregulated in these groups(P＜0.05).Conclusion:SGB can mitigate the inflammatory response in acute peritonitis rats by inhibiting the TLR4/NF-κB signaling pathway through the downregulation of TLR4 and NF-κB(p65).

Objective: To observe the clinical effect of massage combined with warm acupuncture and moxibustion on primary frozen shoulder. Methods: From April 2018 to April 2019, 30 patients with primary frozen shoulder admitted and treated in Wenzhou Hospital of Traditional Chinese Medicine were selected in theresearch.According to different admission time, they were divided into observation group and control group, with 15 patients in each group.The control group was treated with warm acupuncture and moxibustion, while the observation group was combined with massage.The total effective rate and VAS pain score after treatment were compared and observed. Results: The total effective rate in the observation group was 93.33%, which was higher than 73.33% in the control group (χ²=3.294, P<0.05). The VAS score of the observation group was (1.05±0.22)points after treatment, which was lower than (3.13±0.43)points of the control group, the difference between the two groups was statistically significant(t=2.493, P=0.000). Conclusion Massage combined with warm acupuncture and moxibustion in the treatment of primary frozen shoulder is more effective than the single treatment method, which can relieve the pain of patients and is a safe and effective treatment.

Objective To identify significantly differentially expression of rat by genes chip,try to find out the initiating factors and molecular mechanisms that oxidative stress originate or strengthen the steriod-induced necrosis of femoral head(SINFH).Methods Twenty Wistar rats were dived into experimental group and control group randomly.The rats were injected intraperitoneally whith endotoxin,and then injected intramuscularly with high-dose methylprednisolone or saline in experimental group and control group respectively.The mRNA was extracted from the femoral head of rats inevery group,and the cDNA probes were obtained by inverse transcript,and then carried out microarray detection.The quantitative RT-PCR was used to confirm the results of the microarray.Results Histopathological findings revealed that the experimental group rats had femoral head necrosis,trabecular bone disorders,thinning,bone cell necrosis,and the rate of empty lacunae increased,and in control group no femoral head osteonecrosis was found.Total of 27 differentially expressed genes were found,and of which 4 genes(COX6A2,COX4I2,SOD3,and DUSP1)were significantly different.The expression of these 4 genes were down-regulated.The functions of these four genes involved in inhibition of reactive oxygen species(ROS)generation,accelerated removal of ROS and protection tissue from oxidative damage and so on.Conclusion The expression of oxidative stress-related genes in SINFH of rats exist change.COX6A2,COX4I2,SOD3,and DUSP1 are key genes in process of oxidative stress originate or strengthen the SINFH.