Objective:To discuss the effect of Yes-associated protein(YAP)silencing on the proliferation,migration,and invasion capabilities of the human cervical cancer(CC)SiHa cells.Methods:The human CC SiHa cells were cultured in vitro,and the lentiviral YAP shRNA was transfected into the SiHa cells to establish stably transfected YAP-shRNA experimental group(sh-YAP group)and empty plasmid control group(control group).Western blotting method was used to detect the silencing effect of YAP;immunofluorescence method was used to detect the microfilament number and morphology of actin filaments(F-actin)in the cells in both groups;CCK-8 method was used to detect the survival rates of the cells in two groups;Transwell chamber assay and wound healing assay were used to detect the numbers of migration and invasion cells and scratch healing rates of the cells in two groups;Western blotting method was used to detect the expression levels of epithelial-mesenchymal transition(EMT)markers(E-cadherin and Snail),DNA damage repair-related proteins(γ-H2AX),and apoptosis-related proteins[c-MYC and B-cell lymphoma-2(Bcl-2)]in the cells in two groups.Results:The results of lentiviral YAP shRNA transfection into SiHa cells showed that the expression level of YAP protein in the SiHa cells was significantly decreased(P＜0.05).The immunofluorescence results showed that after YAP silencing,the F-actin in SiHa cells was sparse and regularly arranged,with a reduced number of cells and a shriveled appearance.The CCK-8 results showed that compared with control group,the survival rate of the SiHa cells in sh-YAP group was significantly decreased cultured for 24 and 48 h(P＜0.01).The results of Transwell chamber assay and the wound healing assay showed that compared with control group,the numbers of migration and invasion SiHa cells in sh-YAP group were significantly decreased(P＜0.01),and the cell scratch healing rates were signifiantly decreased(P＜0.05).The Western blotting results showed that compared with control group,the expression level of E-cadherin protein in the cells in sh-YAP group was increased(P＜0.05),and the expression levels of c-MYC,Bcl-2,and γ-H2AX proteins were decreased(P＜0.05 or P＜0.01).Conclusion:YAP gene silencing leads to the depolymerization of F-actin in the human CC SiHa cells and regulates the apoptosis and DNA damage repair,potentially reversing the EMT process,thereby inhibiting the proliferation and migration of the tumor cells.

Objective To establish a Kawasaki disease mathematical diagnosis model in order to sup-port clinical decision-making. Methods Children with fever admitted to Shanghai Children's Hospital from Jan-uary 2013 to July 2017 were recruited and were divided into Kawasaki disease group and other disease control groups according to the final clinical diagnosis. The general clinical information and laboratory indicators were compared,a mathematical model was established and evaluated through the logistic regression analysis. Results A total of 1916 children were enrolled in this study,with an average age of 3. 47 ± 2. 83 years. Of these,1085 (56. 6％) were male,831 (43. 4％) were female,479 (25. 0％) were diagnosed with Kawasaki disease and 1099 (75. 0％) were with other diagnosis. Logistic regression analysis included dependent variables and inde-pendent variables,and the results showed that the Hosmer and Lemeshow test of this model was P=0. 944,the difference was not significant,indicating that the fitting equation and the true equation without deviation; age , fever days,ESR,CRP,WBC,ALB and DD dimers were independent risk factors for Kawasaki disease. The pre-dictive equation of Logistic regression is:ln P1-p( )= -7. 337 +2. 163 × CRP+1. 56 × DD+1. 612 × ESR+1. 392+age+1. 724 × days of fever +2. 295 × WBC +0. 808 × ALB. The patient model score and the ROC curve was calculated. The area under the curve was 0. 927 (95％ CI:0. 905-0. 950). When the score was 9,the Youden index was the highest(72. 9％),the sensitivity and specificity were 89. 7％ and 83. 2％. Conclusion The Kawasaki disease diagnosis mathematical model established in this study has good diagnostic efficacy,which need to be confirmed by further large-scale,multicenter studies.