The animal models with chronic limb-threatening ischemia(CLTI)can be used to study the pathophysiological changes of CLTI so as to formulate more effective treatment strategies.In recent years,with the progress of clinical treatment techniques,studies on animal experimental models have been further improved and developed.Currently,the widely used animal models of CLTI include rodents(such as rats and mice)and large animals(such as rabbits and pigs).The methods of establishing CLTI animal model include ligation and excision,arterial electrocoagulation,Ameroid constrictor,and endovascular techniques.This review summarizes the recent major advances in the establishment of CLTI animal models,so as to enable the relevant workers to obtain a clearer understanding of the advantages and disadvantages of different animals and experimental methods,to improve the animal model,and to provide better treatment options for CLTI patients.