The study investigated the specific mechanism by which oxocrebanine, the anti-hepatic cancer active ingredient in Stephania hainanensis, inhibits the proliferation of hepatic cancer cells. Firstly, methyl thiazolyl tetrazolium(MTT) assay, 5-bromodeoxyuridine(BrdU) labeling, and colony formation assay were employed to investigate whether oxocrebanine inhibited the proliferation of HepG2 and Hep3B2.1-7 cells. Propidium iodide(PI) staining was used to observe the oxocrebanine-induced apoptosis of HepG2 and Hep3B2.1-7 cells. Western blot was employed to verify whether apoptotic effector proteins, such as cleaved cysteinyl aspartate-specific protease 3(c-caspase-3), poly(ADP-ribose) polymerase 1(PARP1), B-cell lymphoma-2(Bcl-2), Bcl-2-associated X protein(Bax), Bcl-2 homologous killer(Bak), and myeloid cell leukemia-1(Mcl-1) were involved in apoptosis. Secondly, HepG2 cells were simultaneously treated with oxocrebanine and the autophagy inhibitor 3-methyladenine(3-MA), and the changes in the autophagy marker LC3 and autophagy-related proteins [eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), phosphorylated 4EBP1(p-4EBP1), 70-kDa ribosomal protein S6 kinase(P70S6K), and phosphorylated P70S6K(p-P70S6K)] were determined. The results of MTT assay, BrdU labeling, and colony formation assay showed that oxocrebanine inhibited the proliferation of HepG2 and Hep3B2.1-7 cells in a dose-dependent manner. The results of flow cytometry suggested that the apoptosis rate of HepG2 and Hep3B2.1-7 cells increased after treatment with oxocrebanine. Western blot results showed that the protein levels of c-caspase-3, Bax, and Bak were up-regulated and those of PARP1, Bcl-2, and Mcl-1 were down-regulated in the HepG2 cells treated with oxocrebanine. The results indicated that oxocrebanine induced apoptosis, thereby inhibiting the proliferation of hepatic cancer cells. The inhibition of HepG2 cell proliferation by oxocrebanine may be related to the induction of protective autophagy in hepatocellular carcinoma cells. Oxocrebanine still promoted the conversion of LC3-Ⅰ to LC3-Ⅱ, reduced the phosphorylation levels of 4EBP1 and P70S6K, which can be reversed by the autophagy inhibitor 3-MA. It is prompted that oxocrebanine can inhibit the proliferation of hepatic cancer cells by inducing autophagy. In conclusion, oxocrebanine inhibits the proliferation of hepatic cancer cells by inducing apoptosis and autophagy.
Humans ; Apoptosis/drug effects* ; Autophagy/drug effects* ; Cell Proliferation/drug effects* ; Hep G2 Cells ; Liver Neoplasms/genetics* ; Carcinoma, Hepatocellular/genetics* ; Caspase 3/genetics*

Abstract: Objective To evaluate the key quality control and protective performance test of Halcyon medical linear accelerator. Methods WS 674-2020Specification for Testing of Quality Control in Medical Linear Accelerator（ According to the hereinafter WS 674-2020） ， referred to and the manufacturer´s manual the performance of the first Halcyon medical linear accelerator in Results ， ， Hunan Province was tested. The results showed that all ten indicators of the accelerator including dose deviation ， ， （ ， ， ， - repeatability linearity daily stability and symmetry the results were 0.10% 0.03% 0.04% 0.50% and 100.50% 100.80% ）， - - respectively met the requirements of WS 674 2020. The results of manufacturer quality control indicators such as dose rate ， ， stability in beam gantry rotation isocenter and mechanical position accuracy megavolt image parameters and cone beam computer tomography image parameters met the requirements of the manufacturer´s regulations. Due to the special structure and ， - ： function of the accelerator it is difficult to detect the parameter required by WS 674 2020 as below the radiation leakage - ， ， ， ， outside the M zone the uniformity the indicators related to the light field the offset of the radiation beam axis the zero scale Conclusion - position of the rotating motion scale and others. It is difficult to carry out complete testing according to WS 6742020 for Halcyon medical linear accelerator and it is urgent for the state to issue relevant testing standards to standardize and strengthen the quality control testing of various accelerators.

Objective To detect the dynamic visual acuity ( DVA) before and after vestibular habituation of subjects in order to optimize the DVA assessment criteria .Methods The vestibular function examination system was applied to the detection of static and dynamic visual function in 16 healthy subjects .Results When the speed of left or right swinging was fast enough , DVA before and after vestibular habituation was different .Conclusion Subjects with vestibular habituation can reduce their sensitivity to the vestibular system , the changes in DVA are better than before habituation , and the vestib-ular function adaptability training may have effect on DVA .

Objective To validate the feasibility of applying ground-based spatial disorientation training scenarios to aeromedical training of Chinese military pilots and to provide reference values for assessing its effects. Methods Eighty healthy pilots performed ground-based spatial disorientation training on the basic of previously established aeromedical training profiles of Chinese Air Force and the scenarios applied by North Atlantic Treaty Organization (NATO) were referenced. Somatogyral illusion and Coriolis illusion were induced by VTS-0 type electric rotating chair, while circular vection and autokinetic illusion were induced by VTS-Ⅲ type vestibular function examination system. During the illusion demonstrations, the response parameters of illusion, including the latent period and the duration of induced illusions were recorded. Results The induced rate of somatogyral illusion, Coriolis illusion, circular vection and autokinetic illusion was 100.0%, 90.7%, 72.50% and 49.2% respectively. The induce rate of vestibular illusions was higher than that of visual illusions (χ2=5.01, P<0.05). The duration of somatogyral illusion, Coriolis illusion (rolling sensation) and Coriolis illusion (tumbling sensation) was (8.4±4.5) s, (4.9±2.3) s and (5.4±3.0) s respectively, while the latent period of circular vection and autokinetic illusion was (34.8±19.0) s and (42.8±15.2) s respectively. The response reference value corresponding to the duration of somatogyral illusion, Coriolis illusion (rolling sensation) and Coriolis illusion (tumbling sensation) was respectively not more than 17.4 s, 11.4 s and between 0.3 and 9.5 s, while the response reference value for the latent period of circular vection and autokinetic illusion was respectively not more than 72.8 s and within the range from 12.4 to 73.2 s. Conclusions Somatogyral illusion, Coriolis illusion, circular vection and autokinetic illusion training scenarios can be demonstrated by the existing ground-based training devices of Chinese military pilots and the training is feasible and effective for aeromedical training of Chinese military pilots. We hope that response reference values can be used in assessing the effect of ground-based spatial disorientation training.

Objective To validate the feasibility of applying ground-based spatial disorientation training scenarios to aeromedical training of Chinese military pilots and to provide reference values for assessing its effects. Methods Eighty healthy pilots performed ground-based spatial disorientation training on the basic of previously established aeromedical training profiles of Chinese Air Force and the scenarios applied by North Atlantic Treaty Organization (NATO) were referenced. Somatogyral illusion and Coriolis illusion were induced by VTS-0 type electric rotating chair, while circular vection and autokinetic illusion were induced by VTS-Ⅲ type vestibular function examination system. During the illusion demonstrations, the response parameters of illusion, including the latent period and the duration of induced illusions were recorded. Results The induced rate of somatogyral illusion, Coriolis illusion, circular vection and autokinetic illusion was 100.0%, 90.7%, 72.50% and 49.2% respectively. The induce rate of vestibular illusions was higher than that of visual illusions (χ2=5.01, P<0.05). The duration of somatogyral illusion, Coriolis illusion (rolling sensation) and Coriolis illusion (tumbling sensation) was (8.4±4.5) s, (4.9±2.3) s and (5.4±3.0) s respectively, while the latent period of circular vection and autokinetic illusion was (34.8±19.0) s and (42.8±15.2) s respectively. The response reference value corresponding to the duration of somatogyral illusion, Coriolis illusion (rolling sensation) and Coriolis illusion (tumbling sensation) was respectively not more than 17.4 s, 11.4 s and between 0.3 and 9.5 s, while the response reference value for the latent period of circular vection and autokinetic illusion was respectively not more than 72.8 s and within the range from 12.4 to 73.2 s. Conclusions Somatogyral illusion, Coriolis illusion, circular vection and autokinetic illusion training scenarios can be demonstrated by the existing ground-based training devices of Chinese military pilots and the training is feasible and effective for aeromedical training of Chinese military pilots. We hope that response reference values can be used in assessing the effect of ground-based spatial disorientation training.

Adolescent ; Female ; Gigantism ; diagnosis ; surgery ; Humans ; Limb Salvage ; methods ; Lipomatosis ; diagnosis ; surgery ; Upper Extremity ; pathology ; surgery

OBJECTIVETo investigate the effects of the Chinese herbal medicines Fructus Lycii and Radix Astragali on the function of the Sertoli cells in the rat testis and their mechanisms.

METHODSSertoli cells from the testes of the SD rats aged 18 - 22 days were isolated and cultured. The effects of Fructus Lycii, Radix Astragali and the combined administration of the two on the proliferation of Sertoli cells in vitro were detected by MTT assay, and their effects on the level of INHbetaB mRNA transcription in Sertoli cells in vitro were investigated in both normal environment and peroxide-damaging environment by RT-PCR.

RESULTSThe proliferation of Sertoli cells was promoted by either Fructus Lycii or Radix Astragali at high concentration (P < 0.05), and significantly promoted by the combined administration at high concentration (P <0.01). Sertoli cell INHbetaB transcription was significantly up-regulated by Fructus Lycii, Radix Astragali and their combined administration in vitro (P < 0.01). When the level of INHbetaB mRNA in Sertoli cells significantly dropped (P < 0.01) in the presence of injury induced by peroxide (H2O2), it could be elevated by Radix Astragali (P < 0.05) and significantly up-regulated by Fructus Lycii or the combined administration in vitro (P < 0.01).

CONCLUSIONFructus Lycii, Radix Astragali and the combined administration of the two could promote and protect INHbetaB mRNA in Sertoli cells in vitro.

Animals ; Astragalus membranaceus ; Cell Proliferation ; drug effects ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; pharmacology ; In Vitro Techniques ; Inhibin-beta Subunits ; biosynthesis ; genetics ; Lycium ; Male ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley ; Sertoli Cells ; drug effects