ObjectiveTo explore the effectiveness of feedforward control mode on improving occupational burnout among operating room nurses through theoretical research and clinical practice， with a view to promoting their physical and mental health and enhancing the quality of surgical nursing. MethodsA total of 440 operating room nurses from different regions， scales， and nursing experiences in Shaanxi Province from November 2023 to December 2023 were randomly divided into an experimental group and a control group， with 220 nurses in each group. While the control group received routine intervention measures， the experimental group introduced a feedforward control mode based on the control group， with “emotional exhaustion，” “depersonalization，”and“personal achievement” as observation indicators. ResultsThe incidence of occupational burnout in the experimental group was 11.4%， while that in the control group was 20.0%. The experimental group showed a significantly lower incidence than the control group （P=0.013）. ConclusionThe feedforward control mode can significantly alleviate nurses’ sense of job burnout， promote the improvement of surgical nursing quality， as well as continuously improve the scientific rigor， advancement， and humanistic nature of nursing services， which is conducive to building a harmonious and efficient nursing team. The spirit of teamwork， reflected in mutual support， mutual trust， and joint efforts for surgical success and patient health， has become an important component of nurses’ professional ethics.

As the main body of scientific research and innovation in hospitals,timely understanding of medical person-nel's scientific research outputs and its influencing factors has practical guiding significance for hospital management to identify scientific research talents,formulate targeted training programs,customize relevant science and technology policies,etc.This ar-ticle will comprehensively elaborate the concept of scientific research outputs,evaluation methods and influencing factors of scien-tific research outputs among medical personnel.Meanwhile,the article will provide suggestions for influencing factors,in order to provide references for the hospital management in the future.

Esophageal squamous cell carcinoma(ESCC)often exhibits a high incidence of multifocality.The dis-tinction between multiple primary and metastatic tumor origin is a prerequisite for precise diagnosis and treatment.Based on histopathological diagnosis,molecular pathological diagnostic indicators and technologies utilizing multi-omics approaches(DNA,RNA,protein)have been gradually identified.This article systematically reviews recent advance-ments in pathological diagnostic techniques for distinguishing the origin of multifocal ESCC and provides future perspec-tives,aiming to offer references for precise diagnosis.

AIM:This study aimed to establish a model of functional impairment in INS-1 cells(rat insulino-ma cells)induced by prolonged exposure to a low level of interleukin-1β(IL-1β),and to investigate the underlying mech-anisms.METHODS:INS-1 cells were treated with different concentrations of IL-1β for 10 d.The cells were divided into control,0.05 μg/L IL-1β,0.1 μg/L IL-1β,and 0.2 μg/L IL-1β groups(n=3).Flow cytometry was employed to assess cell apoptosis,facilitating the identification of IL-1β concentrations that did not significantly induce apoptosis.Subse-quently,INS-1 cells were divided into control and IL-1β groups(n=3)and treated with 0.1 μg/L IL-1β for 10 days.The intracellular insulin content and glucose-stimulated insulin secretion level were detected by immunofluorescence and en-zyme-linked immunosorbent assay(ELISA).Differential proteins and associated signaling pathways were screened using 4D label-free proteomics technology.Moreover,fluorescent probes were used to detect glucose-stimulated calcium ion in-flux,and Western blot was conducted to verify the expression changes of relevant proteins in the nuclear factor kappa-B(NF-κB)signaling pathway and calcium signaling pathway.RESULTS:Treatment with 0.1 μg/L IL-1β for 10 days ex-hibited minimal impact on the survival of INS-1 cells but significantly reduced the intracellular insulin content and glucose-stimulated insulin secretion(P<0.01).Therefore,0.1 μg/L IL-1β was chosen for in vitro induction of functional impair-ment in INS-1 cells.Proteomic analysis showed no significant differences in the expression of apoptosis-related factors,such as caspase-3,B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),and Bcl-2-associated death promoter(Bad),compared with the control(P>0.05).However,the expression of insulin,v-maf avian musculoaponeurotic fibro-sarcoma oncogene family protein A(Mafa),calcium/calmodulin-dependent protein kinase II(CaMKII),inositol 1,4,5-triphosphate receptor(IP3R),and NF-kappa-B inhibitor alpha(IκBα)was significantly reduced(P<0.01),while the protein expression levels of NF-κB and inducible nitric oxide synthase(iNOS)were significantly increased(P<0.05,P<0.01).Fluorescence probe results showed that,compared to the control,the calcium ion influx induced by glucose stimu-lation in the IL-1β group was significantly reduced(P<0.01).Western blot results demonstrated that the expression lev-els of IP3R,CaMKII,and IκBα in the IL-1β group were significantly reduced(P<0.05,P<0.01),while the expression levels of NF-κB and iNOS were significantly increased(P<0.01).CONCLUSION:A model of functional impairment in rat INS-1 cells induced by prolonged exposure to low levels of IL-1β has been successfully established,and it has been pre-liminarily confirmed that this impairment may be related to the activation of the NF-κB signaling pathway and dysfunction of the calcium signaling pathway.

Objective:To conduct a scoping review of studies related to the application of dance movement therapy (DMT) in cancer patients to provide a reference for subsequent research and clinical promotion of its application.Methods:Using the research method of scoping review, domestic and international databases such as China National Knowledge Infrastructure, Wanfang, PubMed, Embase, etc. were systematically searched, with a search timeframe from the construction of the database to April 12, 2025, and the included literature was screened, summarized, and analyzed and the findings were reported.Results:A total of 12 papers were finally included, including 7 randomized controlled trials and 5 quasi-experimental studies. The frequency of DMT interventions was mostly 2 times per week, each intervention usually lasts for 1-1.5 h, for a duration ranging from 2 weeks to 3 months, and was mostly implemented by professionally qualified dance movement therapists, which could effectively improve the physical functioning, mental state, quality of life, and cancer-related symptoms of cancer patients.Conclusions:DMT, as a flexible and creative psychotherapy, has positive significance in cancer patients.

Objective To explore the prognosis-related genes of lung adenocarcinoma(LUAD)and establish a prognostic prediction model for LUAD.Methods The differentially expressed genes of LUAD tissues and adjacent normal tissues in the GSE43458,GSE7670,and GSE140797 datasets were screened.The protein-protein interaction(PPI)network analysis,gene ontology(GO)function,Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathway enrichment analyses,least absolute shrinkage and selection operator for prognosis coefficient screening,disease-specific survival analysis,Cox regression analysis,and gene correlation analysis were performed.Independent prognostic genes of LUAD were screened from the differential genes,and a prognostic prediction model of LUAD was established.The expression of independent prognostic genes was analyzed,and the predictive model was evaluated by receiver operating characteristic(ROC)curve.The GSE68465 data set was used as an external validation set to verify the predictive model.Results There were 197 up-regulated differential genes and 77 down-regulated differential genes in LUAD and adjacent normal tissues common to the three datasets.Through stepwise screening,two genes,IL7R and SLC2A1,were identified as independent prognostic factors for LUAD.IL7R was an independent protective factor,while the SLC2A1 was an independent risk factor.A prediction model for curve was built with IL7R and SLC2A1.The prediction model for LUAD constructed with IL7R and SLC2A1 is as follows:Risk score=(-0.694)×expression level of IL7R+0.763×expression level of SLC2A1.Conclusion This study screened out IL7R as an independent prognostic protective factor for LUDA,and SLC2A1 as an independent prognostic risk factor for LUAD.The LUDA prediction model constructed based on these two genes has good predictive ability and generalization ability,which can provide references for the research and clinical individualized treatment of LUAD.

As the main body of scientific research and innovation in hospitals,timely understanding of medical person-nel's scientific research outputs and its influencing factors has practical guiding significance for hospital management to identify scientific research talents,formulate targeted training programs,customize relevant science and technology policies,etc.This ar-ticle will comprehensively elaborate the concept of scientific research outputs,evaluation methods and influencing factors of scien-tific research outputs among medical personnel.Meanwhile,the article will provide suggestions for influencing factors,in order to provide references for the hospital management in the future.

Esophageal squamous cell carcinoma(ESCC)often exhibits a high incidence of multifocality.The dis-tinction between multiple primary and metastatic tumor origin is a prerequisite for precise diagnosis and treatment.Based on histopathological diagnosis,molecular pathological diagnostic indicators and technologies utilizing multi-omics approaches(DNA,RNA,protein)have been gradually identified.This article systematically reviews recent advance-ments in pathological diagnostic techniques for distinguishing the origin of multifocal ESCC and provides future perspec-tives,aiming to offer references for precise diagnosis.

AIM:This study aimed to establish a model of functional impairment in INS-1 cells(rat insulino-ma cells)induced by prolonged exposure to a low level of interleukin-1β(IL-1β),and to investigate the underlying mech-anisms.METHODS:INS-1 cells were treated with different concentrations of IL-1β for 10 d.The cells were divided into control,0.05 μg/L IL-1β,0.1 μg/L IL-1β,and 0.2 μg/L IL-1β groups(n=3).Flow cytometry was employed to assess cell apoptosis,facilitating the identification of IL-1β concentrations that did not significantly induce apoptosis.Subse-quently,INS-1 cells were divided into control and IL-1β groups(n=3)and treated with 0.1 μg/L IL-1β for 10 days.The intracellular insulin content and glucose-stimulated insulin secretion level were detected by immunofluorescence and en-zyme-linked immunosorbent assay(ELISA).Differential proteins and associated signaling pathways were screened using 4D label-free proteomics technology.Moreover,fluorescent probes were used to detect glucose-stimulated calcium ion in-flux,and Western blot was conducted to verify the expression changes of relevant proteins in the nuclear factor kappa-B(NF-κB)signaling pathway and calcium signaling pathway.RESULTS:Treatment with 0.1 μg/L IL-1β for 10 days ex-hibited minimal impact on the survival of INS-1 cells but significantly reduced the intracellular insulin content and glucose-stimulated insulin secretion(P<0.01).Therefore,0.1 μg/L IL-1β was chosen for in vitro induction of functional impair-ment in INS-1 cells.Proteomic analysis showed no significant differences in the expression of apoptosis-related factors,such as caspase-3,B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),and Bcl-2-associated death promoter(Bad),compared with the control(P>0.05).However,the expression of insulin,v-maf avian musculoaponeurotic fibro-sarcoma oncogene family protein A(Mafa),calcium/calmodulin-dependent protein kinase II(CaMKII),inositol 1,4,5-triphosphate receptor(IP3R),and NF-kappa-B inhibitor alpha(IκBα)was significantly reduced(P<0.01),while the protein expression levels of NF-κB and inducible nitric oxide synthase(iNOS)were significantly increased(P<0.05,P<0.01).Fluorescence probe results showed that,compared to the control,the calcium ion influx induced by glucose stimu-lation in the IL-1β group was significantly reduced(P<0.01).Western blot results demonstrated that the expression lev-els of IP3R,CaMKII,and IκBα in the IL-1β group were significantly reduced(P<0.05,P<0.01),while the expression levels of NF-κB and iNOS were significantly increased(P<0.01).CONCLUSION:A model of functional impairment in rat INS-1 cells induced by prolonged exposure to low levels of IL-1β has been successfully established,and it has been pre-liminarily confirmed that this impairment may be related to the activation of the NF-κB signaling pathway and dysfunction of the calcium signaling pathway.

Objective:To conduct a scoping review of studies related to the application of dance movement therapy (DMT) in cancer patients to provide a reference for subsequent research and clinical promotion of its application.Methods:Using the research method of scoping review, domestic and international databases such as China National Knowledge Infrastructure, Wanfang, PubMed, Embase, etc. were systematically searched, with a search timeframe from the construction of the database to April 12, 2025, and the included literature was screened, summarized, and analyzed and the findings were reported.Results:A total of 12 papers were finally included, including 7 randomized controlled trials and 5 quasi-experimental studies. The frequency of DMT interventions was mostly 2 times per week, each intervention usually lasts for 1-1.5 h, for a duration ranging from 2 weeks to 3 months, and was mostly implemented by professionally qualified dance movement therapists, which could effectively improve the physical functioning, mental state, quality of life, and cancer-related symptoms of cancer patients.Conclusions:DMT, as a flexible and creative psychotherapy, has positive significance in cancer patients.