Objective To analyze challenges in translating exercise management evidence for patients with metabolism-associated fatty liver disease(MAFLD),develop actionable strategies,and evaluate the application of best evidence.Methods Utilizing the evidence translation model,the best evidence was implemented for MAFLD patients in 4 phases:evidence acquisition,baseline practice review,intervention,and outcome evaluation.We compared the knowledge of exercise management evidence,implementation rates of review indicators,completion of exercise programs,BMI,liver stiffness measurement,controlled attenuation parameters,and patient satisfaction among medical staff at a tertiary hospital in Fujian Province during baseline(March-May 2023),mid-practice(June-August 2023),and late-practice(September-November 2023)phases.Results A total of 88 patients were included at baseline review,95 during mid-practice,and 107 in late-practice.Significant improvements were observed in the implementation rates of 21 review indicators,nurses'knowledge,completion rate,BMI,and controlled attenuation parameters compared to the data at baseline(P＜0.05).Conclusion The application of best evidence in exercise management for MAFLD patients enhances nurses'knowledge,standardizes nursing practices,and reduces patients'BMI and controlled attenuation parameters.

Objective To analyze challenges in translating exercise management evidence for patients with metabolism-associated fatty liver disease(MAFLD),develop actionable strategies,and evaluate the application of best evidence.Methods Utilizing the evidence translation model,the best evidence was implemented for MAFLD patients in 4 phases:evidence acquisition,baseline practice review,intervention,and outcome evaluation.We compared the knowledge of exercise management evidence,implementation rates of review indicators,completion of exercise programs,BMI,liver stiffness measurement,controlled attenuation parameters,and patient satisfaction among medical staff at a tertiary hospital in Fujian Province during baseline(March-May 2023),mid-practice(June-August 2023),and late-practice(September-November 2023)phases.Results A total of 88 patients were included at baseline review,95 during mid-practice,and 107 in late-practice.Significant improvements were observed in the implementation rates of 21 review indicators,nurses'knowledge,completion rate,BMI,and controlled attenuation parameters compared to the data at baseline(P＜0.05).Conclusion The application of best evidence in exercise management for MAFLD patients enhances nurses'knowledge,standardizes nursing practices,and reduces patients'BMI and controlled attenuation parameters.

OBJECTIVES: To explore ferroptosis-related genes in osteoporosis through bioinformatic analysis and in vitro study. METHODS: Osteoporosis-related genes were identified from dataset GSE35958 in the Gene Expression Omnibus database; and the ferroptosis-related genes were identified from the FerrDb database. These were intersected with the differentially expressed genes in GSE35958 to obtain ferroptosis-related genes in osteoporosis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed for the differentially expressed genes. And Spearman correlation and protein-protein interaction network analysis were performed. Then, the hub genes of ferroptosis in osteoporosis were screened by Degree, MNC, EPC, MCC and DMNC in Cytoscape software CytoHubba plugin; and analyzed with receiver operating characteristic (ROC) curves. The bone marrow mesenchymal stem cells from osteoporosis patients (osteoporosis group) and non-osteoporosis patients (control group) were subjected to quantitative reverse transcription polymerase chain reaction to detect the messenger RNA expression of ferroptosis hub genes in both groups. RESULTS: A total of 32 differentially expressed genes related to ferroptosis in osteoporosis were identified, including 26 up-regulated genes and 6 down-regulated genes. GO enrichment analysis showed that the identified genes were mainly involved in intercellular adhesion, lipid metabolism and cytokine response. KEGG enrichment analysis showed that the genes were mainly involved in signaling pathways of adhesive plaques, MAPK, PI3K-Akt, and Wnt. Spearman correlation analysis showed correlation among differentially expressed genes. Six hub genes for ferroptosis in osteoporosis were obtained, namely MAPK3, CDKN1A, MAP1LC3A, TNF, RELA, and TGF-β1. ROC curve analysis showed that these hub genes had good diagnostic performance in osteoporosis and may become potential biomarkers of osteoporosis. In vitro experiments confirmed significant differences in these hub genes between the control group and the osteoporosis group (all P<0.05). CONCLUSIONS This study has identified six ferroptosis-related hub genes in osteoporosis, which may be used as novel biomarkers for the early diagnosis and treatment of osteoporosis.
Osteoporosis/genetics* ; Humans ; Computational Biology ; Ferroptosis/genetics* ; Protein Interaction Maps/genetics* ; Gene Ontology ; Mesenchymal Stem Cells/metabolism* ; Gene Expression Profiling ; Databases, Genetic

Objectives:To explore the trend of the burden of cardiovascular disease (CVD) in adult Chinese population attributed to high body mass index (BMI) from 1990 to 2021,identify key populations requiring for BMI control,and provide scientific evidence for developing targeted intervention strategies.Methods:Based on the Global Burden of Disease (GBD) 2021 database,we compared the age-standardized disability-adjusted life years (DALY) rates and age-standardized mortality rates of CVD attributed to high BMI for the adult population aged ≥20 years in China,globally,and in the United States,the United Kingdom,Japan,and India from 1990 to 2021.Additionally,the results were stratified by sex and age to compare the trends of disease burden in China and globally.Results:From 1990 to 2021,the average annual percentage change (AAPC) in age-standardized DALY rate and age-standardized mortality rate for CVD attributable to high BMI for the population aged ≥20 years in China was 1.08 (95％CI:1.05 to 1.11) and 1.00 (95％CI:0.96 to 1.03),respectively.Throughout the 32-year period,both rates were consistently lower than those in the United States and the global average,but higher than those in India and Japan.Meanwhile,England experienced a continuous decline,with corresponding data of-3.02 (95％CI:-3.10 to-2.96) for AAPC in age-standardized DALY rates and-3.02 (95％CI:-3.11 to-2.95) for AAPC in age-standardized mortality rates,respectively.The age-standardized DALY rate attributed to high BMI for adult males in China was consistently higher than those for females,whereas the percentage of DALY showed the opposite trend,with females surpassing males.The age-standardized DALY rates for the population aged ≥20 years attributed to high BMI in China increased continuously,and the percentage of DALY increased sharply in population aged 20-39,the level of 2021 was more than three times higher as compared to 1990.Conclusions:The increasing proportion of overweight and obese individuals in China,along with the resulting rise in the prevalence and mortality of cardiovascular diseases,has led to a continuous increase in the cardiovascular disease burden.It is therefore particularly important to implement effective public health interventions to control overweight in the young and middle-aged population to reduce the burden of cardiovascular disease in China.

Objectives:To explore the trend of the burden of cardiovascular disease (CVD) in adult Chinese population attributed to high body mass index (BMI) from 1990 to 2021,identify key populations requiring for BMI control,and provide scientific evidence for developing targeted intervention strategies.Methods:Based on the Global Burden of Disease (GBD) 2021 database,we compared the age-standardized disability-adjusted life years (DALY) rates and age-standardized mortality rates of CVD attributed to high BMI for the adult population aged ≥20 years in China,globally,and in the United States,the United Kingdom,Japan,and India from 1990 to 2021.Additionally,the results were stratified by sex and age to compare the trends of disease burden in China and globally.Results:From 1990 to 2021,the average annual percentage change (AAPC) in age-standardized DALY rate and age-standardized mortality rate for CVD attributable to high BMI for the population aged ≥20 years in China was 1.08 (95％CI:1.05 to 1.11) and 1.00 (95％CI:0.96 to 1.03),respectively.Throughout the 32-year period,both rates were consistently lower than those in the United States and the global average,but higher than those in India and Japan.Meanwhile,England experienced a continuous decline,with corresponding data of-3.02 (95％CI:-3.10 to-2.96) for AAPC in age-standardized DALY rates and-3.02 (95％CI:-3.11 to-2.95) for AAPC in age-standardized mortality rates,respectively.The age-standardized DALY rate attributed to high BMI for adult males in China was consistently higher than those for females,whereas the percentage of DALY showed the opposite trend,with females surpassing males.The age-standardized DALY rates for the population aged ≥20 years attributed to high BMI in China increased continuously,and the percentage of DALY increased sharply in population aged 20-39,the level of 2021 was more than three times higher as compared to 1990.Conclusions:The increasing proportion of overweight and obese individuals in China,along with the resulting rise in the prevalence and mortality of cardiovascular diseases,has led to a continuous increase in the cardiovascular disease burden.It is therefore particularly important to implement effective public health interventions to control overweight in the young and middle-aged population to reduce the burden of cardiovascular disease in China.

Objective To explore the change of proportion of peripheral blood dendritic cells (DCs) in patients with stroke. Methods 56 patients (30 cases of cerebral infarction and 26 cases of cerebral hemorrhage) in Beijing Bo'ai hospital from June to September, 2014 and 14 healthy controls were investigated. The severity of stroke was assessed with the National Institutes of Health Stroke Scale (NIHSS). Flow cy-tometry analysis was employed to detect the proportion of DCs subtypes in the peripheral blood. Results No obvious difference was found in DCs between the stroke patients and the controls. Compared to the control group, the percentages of peripheral blood myeloid dendritic cells (mDCs) decreased in the cerebral hemorrhage and the cerebral infarction subgroups (P<0.001). The percentages of plasmacytoid den-dritic cells (pDCs) reduced significantly in the cerebral hemorrhage and the cerebral infarction subgroups (P<0.05). The stroke patients were divided into NIHSS≤7 subgroup and NIHSS>7 subgroup. The percentages of pDCs in the cerebral hemorrhage and the cerebral infarction patients were significantly lower in the NIHSS>7 subgroup than in the NIHSS≤7 subgroup (P<0.05). While there was no statistical differ-ence between NIHSS≤7 subgroups and NIHSS>7 subgroups in the percentages of mDCs in the cerebral hemorrhage and cerebral infarction patients. Conclusion The proportion of DCs subtypes in the peripheral blood in stroke patients changed significantly, indicating inflamma-tion responds play a role in stroke.

Objective To predict the B cell epitope for MUC1 antigen. Methods The secondary structure and surface properties of MUC1, such as physical and chemical characters, hydrophilicity, antigenicity, were analyzed with various methods. Results Many distinct antigenic epitopes in MUC1 were identified by computation: 1-10, 24-54, 65-77, 84-91, 108-134, 140-156, 159-174, 179-196, 199-210, 220-233, 237-265, 270-299, 316-337, 351-362, 369-396, 411-420, and 445-502. Conclusion Prediction of the B cell epitope for MUC1 can provide a base for the studies of structure and function of MUC1, construction of its mutants, and selection of new expression forms of MUC1.