Objective:To investigate the effects of mirror therapy(MT)combined with kinesio taping(KT)on persistent pain and quality of life after breast cancer treatment.Method:Patients with persistent pain after breast cancer treatment were recruited from January 2022 to Decem-ber 2022 from Department of Rehabilitation Medicine,Jing'an District Central Hospital of Shanghai.A total of 43 patients were randomized into the combination group(MT+KT,n=22)and the KT group(n=21).Patients in both groups received conventional physical rehabilitation therapy.Additionally,the KT group received kinesio tap-ing and sham mirror therapy,while the combined group received kinesio taping and mirror therapy.Before treat-ment and after 4 weeks of treatment,numerical rating scales(NRSs)was used to evaluate pain,bilateral grip strength was measured using J-Tech grip strength device,shoulder range of motion(ROM)was measured using the goniometer,and quality of life was measured using the short form-36(SF-36)Result:There were no significant differences in NRSs score,grip strength difference,shoulder flexion and ab-duction difference,and SF-36 score between two groups before treatment(P＞0.05).After 4 weeks of interven-tion,the NRSs score,shoulder flexion and shoulder abduction of the combined group were significantly im-proved compared with those before treatment(P＜0.05).The NRSs score,shoulder flexion and shoulder abduc-tion in group KT were significantly improved compared with those before treatment(P＜0.05).Comparison be-tween groups showed that NRSs score of combined group was significantly lower than that of group KT(P=0.001),although grip strength,shoulder flexion and abduction of combined group were improved compared with group KT,but the difference was no statistically significant(P＞0.05).After 4 weeks of intervention,the scores of physiological function,general health and emotional function of SF-36 in combination group were significantly higher than those in group KT(P＜0.05).Conclusion:Mirror therapy combined with kinesio taping can effectively improve the persistent pain and spe-cific aspects of the quality of life in patients after breast cancer treatment.

Objective:To investigate the effects of mirror therapy(MT)combined with kinesio taping(KT)on persistent pain and quality of life after breast cancer treatment.Method:Patients with persistent pain after breast cancer treatment were recruited from January 2022 to Decem-ber 2022 from Department of Rehabilitation Medicine,Jing'an District Central Hospital of Shanghai.A total of 43 patients were randomized into the combination group(MT+KT,n=22)and the KT group(n=21).Patients in both groups received conventional physical rehabilitation therapy.Additionally,the KT group received kinesio tap-ing and sham mirror therapy,while the combined group received kinesio taping and mirror therapy.Before treat-ment and after 4 weeks of treatment,numerical rating scales(NRSs)was used to evaluate pain,bilateral grip strength was measured using J-Tech grip strength device,shoulder range of motion(ROM)was measured using the goniometer,and quality of life was measured using the short form-36(SF-36)Result:There were no significant differences in NRSs score,grip strength difference,shoulder flexion and ab-duction difference,and SF-36 score between two groups before treatment(P＞0.05).After 4 weeks of interven-tion,the NRSs score,shoulder flexion and shoulder abduction of the combined group were significantly im-proved compared with those before treatment(P＜0.05).The NRSs score,shoulder flexion and shoulder abduc-tion in group KT were significantly improved compared with those before treatment(P＜0.05).Comparison be-tween groups showed that NRSs score of combined group was significantly lower than that of group KT(P=0.001),although grip strength,shoulder flexion and abduction of combined group were improved compared with group KT,but the difference was no statistically significant(P＞0.05).After 4 weeks of intervention,the scores of physiological function,general health and emotional function of SF-36 in combination group were significantly higher than those in group KT(P＜0.05).Conclusion:Mirror therapy combined with kinesio taping can effectively improve the persistent pain and spe-cific aspects of the quality of life in patients after breast cancer treatment.

Objective To solve the problem of castration resistant prostate cancer (castrationresistant prostate cancer,CRPC) the problem of drug resistance by studing the expression of the CUDC-101 inhibitor of castration resistant prostate cancer androgen receptor splice variant 7.Methods In this study,the expression of AR-V7 protein in prostate cancer cell lines PC-3,VCaP,22Rv1,LNCap was detected by imnmunoblotting between April 2015 and April 2016,and the highest expression level of cell lines was selected follow-up experiments.Through the cell proliferation and activity experiments,the epigenetic inhibitors:histone deacetylase inhibitor CUDC-101,histone methylation inhibitor DZNeP,DNA methylation inhibitor gemcitabine,histone acetyltransferase inhibitor MG149 to select an inhibitor that reduces the expression of AR-V7 protein in CRPC cells.22Rv1 cells were treated with 30 nmol and 300 nmol of CUDC101 and 1,10 and 20 μmol of Enzalutamide (MDV3100),respectively,and their inhibitory effects on the growth of 22Rv1 cells were examined.Results The results of immunoblotting showed that AR-V7 protein was only expressed in CRPC cell line 22Rv1 and negative in non-CRPC cell line.The expression of AR-V7 in 22Rv1 cells treated with CUDC-101 was significantly lower than that of negative control.While other inhibitors had no effect on the expression of AR-V7.In the cell proliferation and activity assay,the inhibitory rates of 30 nmol CUDC-101 and 1,10 and 20 μmol MDV3100 were 10％,35％ and 45％,respectively,higher than that of MDV3100 alone.28％ and 42％,the difference was statistically significant (P ＜ 0.05).The inhibitory rates of 300 nmol CUDC-101 and MDV3100 were 30％,60％ and 65％,respectively,which were significantly higher than those of MDV3100 alone (P ＜ 0.05).Conclusions CUDC-101 can inhibit the castration resistant prostate cancer androgen receptor splice variant 7 expression,and solved the resistance problem of CRPC.