Objective·To employ the two-sample Mendelian randomization(TSMR)method,using genetic variants as instrumental variables,to investigate the causal relationship between phosphatidylethanolamine(PE)and the risk of colorectal adenocarcinoma.Methods·The single-nucleotide polymorphism(SNP)data associated with PE and colorectal adenocarcinoma were obtained from the Medical Research Council Integrative Epidemiology Unit(MRC IEU)at the University of Bristol and the Finnish Biobank,respectively.A secondary data analysis was conducted using summary statistics from genome-wide association studies(GWAS),and genetic loci strongly associated with PE were selected as instrumental variables.Four Mendelian randomization(MR)methods,inverse-variance weighted(IVW)method,MR-Egger regression,weighted median(WME)method,and weighted mode(WM)method,were employed to assess the causal effect.The IVW method was used as the primary statistical approach,while MR-Egger,WME,and WM served as supplementary methods.Rigorous assessments for robustness included MR-Egger regression,MR-PRESSO global tests for horizontal pleiotropy,and Cochran's Q test to evaluate heterogeneity.Results·Ten instrumental variables were selected,and the Steiger test indicated that all PE-associated SNPs exhibited a consistent direction of causal effect on colorectal cancer.Among the 10 SNPs,rs102275 and rs9393903 showed the strongest positive associations with colorectal adenocarcinoma risk,with effect sizes of 0.45(P=8.01×10-5)and 0.82(P=2.31×10-2),respectively.Consistent findings from MR analyses demonstrated that PE elevated the risk of colorectal adenocarcinoma across all four methods.In the IVW analysis,the OR was 1.36(95%CI 1.17?1.59,P=7.24×10-5).In the MR-Egger regression,the OR was 1.44(95%CI 0.97?2.14,P=1.12×10-1).In the WEM analysis,the OR was 1.33(95%CI 1.07?1.65,P=8.81×10-3).In the WM analysis,the OR was 1.41(95%CI 1.12?1.77,P=1.70×10-2).Cochran's Q test revealed no heterogeneity among the effect estimates of the 10 SNPs on colorectal adenocarcinoma.Both MR-Egger regression intercept and MR-PRESSO test indicated no evidence of horizontal pleiotropy among the SNPs.Leave-one-out analysis showed overlapping confidence intervals after excluding any single SNP,indicating that the results were not sensitive to individual SNPs and were highly robust.Conclusions·There is a causal association between circulating PE levels and the risk of colorectal adenocarcinoma.A genetically predicted increase of one standard deviation in plasma PE levels is associated with a 1.36-fold higher risk of developing colorectal adenocarcinoma(95%CI 1.17?1.59,P=7.24×10-5).

Objective·To employ the two-sample Mendelian randomization(TSMR)method,using genetic variants as instrumental variables,to investigate the causal relationship between phosphatidylethanolamine(PE)and the risk of colorectal adenocarcinoma.Methods·The single-nucleotide polymorphism(SNP)data associated with PE and colorectal adenocarcinoma were obtained from the Medical Research Council Integrative Epidemiology Unit(MRC IEU)at the University of Bristol and the Finnish Biobank,respectively.A secondary data analysis was conducted using summary statistics from genome-wide association studies(GWAS),and genetic loci strongly associated with PE were selected as instrumental variables.Four Mendelian randomization(MR)methods,inverse-variance weighted(IVW)method,MR-Egger regression,weighted median(WME)method,and weighted mode(WM)method,were employed to assess the causal effect.The IVW method was used as the primary statistical approach,while MR-Egger,WME,and WM served as supplementary methods.Rigorous assessments for robustness included MR-Egger regression,MR-PRESSO global tests for horizontal pleiotropy,and Cochran's Q test to evaluate heterogeneity.Results·Ten instrumental variables were selected,and the Steiger test indicated that all PE-associated SNPs exhibited a consistent direction of causal effect on colorectal cancer.Among the 10 SNPs,rs102275 and rs9393903 showed the strongest positive associations with colorectal adenocarcinoma risk,with effect sizes of 0.45(P=8.01×10-5)and 0.82(P=2.31×10-2),respectively.Consistent findings from MR analyses demonstrated that PE elevated the risk of colorectal adenocarcinoma across all four methods.In the IVW analysis,the OR was 1.36(95%CI 1.17?1.59,P=7.24×10-5).In the MR-Egger regression,the OR was 1.44(95%CI 0.97?2.14,P=1.12×10-1).In the WEM analysis,the OR was 1.33(95%CI 1.07?1.65,P=8.81×10-3).In the WM analysis,the OR was 1.41(95%CI 1.12?1.77,P=1.70×10-2).Cochran's Q test revealed no heterogeneity among the effect estimates of the 10 SNPs on colorectal adenocarcinoma.Both MR-Egger regression intercept and MR-PRESSO test indicated no evidence of horizontal pleiotropy among the SNPs.Leave-one-out analysis showed overlapping confidence intervals after excluding any single SNP,indicating that the results were not sensitive to individual SNPs and were highly robust.Conclusions·There is a causal association between circulating PE levels and the risk of colorectal adenocarcinoma.A genetically predicted increase of one standard deviation in plasma PE levels is associated with a 1.36-fold higher risk of developing colorectal adenocarcinoma(95%CI 1.17?1.59,P=7.24×10-5).

Colorectal cancer (CRC) represents a significant global health challenge as a common malignancy of the digestive tract. The involvement of B vitamins—specifically folic acid (B9), riboflavin (B2), pyridoxine (B6), and cobalamin (B12)—is crucial in metabolic processes by mediating the transfer of one-carbon (1C) units, which plays a fundamental role in cellular functions and tumor growth. 1C metabolism is involved in synthesis of proteins, lipids, nucleic acids, and other cofactors. 1C metabolism, intertwined with the metabolism of other nutrients, forms complex pathways where B vitamins act as precursors or coenzymes, influencing the production of various intermediates. These vitamins, as essential nutrients, are implicated to varying the pathogenesis and progression of colorectal cancer such as epigenetics. Furthermore, 1C metabolism affects tumor cell fate through multiple aspects including nucleotide synthesis, redox homeostasis, and the interaction with gut microbiota. Given these roles, understanding and monitoring B vitamin levels and their metabolic pathways are essential for colorectal cancer prevention and management. This approach not only helps in reducing tumor-related mortality but also opens new avenues for research into CRC mechanisms and potential therapeutic strategies.

Colorectal cancer (CRC) represents a significant global health challenge as a common malignancy of the digestive tract. The involvement of B vitamins—specifically folic acid (B9), riboflavin (B2), pyridoxine (B6), and cobalamin (B12)—is crucial in metabolic processes by mediating the transfer of one-carbon (1C) units, which plays a fundamental role in cellular functions and tumor growth. 1C metabolism is involved in synthesis of proteins, lipids, nucleic acids, and other cofactors. 1C metabolism, intertwined with the metabolism of other nutrients, forms complex pathways where B vitamins act as precursors or coenzymes, influencing the production of various intermediates. These vitamins, as essential nutrients, are implicated to varying the pathogenesis and progression of colorectal cancer such as epigenetics. Furthermore, 1C metabolism affects tumor cell fate through multiple aspects including nucleotide synthesis, redox homeostasis, and the interaction with gut microbiota. Given these roles, understanding and monitoring B vitamin levels and their metabolic pathways are essential for colorectal cancer prevention and management. This approach not only helps in reducing tumor-related mortality but also opens new avenues for research into CRC mechanisms and potential therapeutic strategies.

Objective:To evaluate the impact of self-crosslinked hyaluronic acid (SCH) gel on endometrium recovery after artificial abortion.Methods:A multicenter, prospective randomized controlled trial was conducted across 18 hospitals from December 2021 to February 2023, involving 382 women who underwent artificial abortion. Participants were randomly allocated to receive either treatment with SCH gel (SCH group) or no treatment (control group) in a 1∶1 ratio. The primary outcome was endometrium thickness in 14 to 18 days after the first postoperative menstruation. Secondary outcomes included changes in menstrual volume during the first postoperative menstruation, menstruation resumption within 6 postoperative weeks, time to menstruation resumption, duration of the first postoperative menstruation, and incidence of dysmenorrhea.Results:Baseline characteristics of participants were comparable between the two groups (all P>0.05), with 95.3% (182/191) in SCH group and 92.7% (177/191) in the control group completed the study. The postoperative endometrial thickness in SCH group was significantly greater than that in the control group [(9.78±3.15) vs (8.95±2.32) mm; P=0.005]. SCH group also had significantly fewer participants with reduced menstrual volume [23 cases (12.6%, 23/182) vs 31 cases (17.5%, 31/177); P=0.038]. Although SCH group experienced less dysmenorrhea during the first postoperative menstrual period, this difference was not statistically significant [28.5% (51/179) vs 37.1% (65/175); P=0.083]. Outcomes were similar between SCH group and the control group regarding the proportion of participants who resumed menstruation within 6 weeks postoperatively, time to menstruation resumption, and duration of the first postoperative menstruation ( P=0.792, 0.485, and 0.254, respectively). No serious adverse events were observed during the study period, and no adverse events were attributed to SCH gel treatment. Conclusion:The application of SCH gel after artificial abortion is safe and might aid in the recovery of the endometrium.

Objective    To explore coronary angiographic characteristics in patients with symptomatic recurrence after coronary artery bypass grafting (CABG). Methods    We performed a retrospective study of 997 patients with symptomatic recurrence after CABG in Beijing Anzhen Hospital from 2010 to 2020. There were 762 males and 235 females, with an average age of 62.41±8.70 years. Results    There was a high prevalence of risk factors like hypertension, diabetes and a history of smoking. Diseased arterial grafts accounted for 27.44% while saphenous vein graft 54.40%; 240 (24.07%) patients had all patent grafts. The main lesion characteristics of diseased grafts were chronic total occlusion lesions (79.57%). Most patients had more diseased native vessels after CABG than before. The type C coronary artery disease in native vessels relevant to ischemic area occurred in 674 (67.60%) patients; 525 (52.66%) patients with recurrent symptom after CABG had both diseased grafts and diseased native vessels. Conclusion     Graft status in patients with symptomatic recurrence after CABG is worse than we expected. The majority have newly developed lesions both in grafts and native vessels. Native vascular lesions will continue to progress after CABG.

Objective:To explore the effect of self-made mold in the spreading moxibustion of patients in the Department of Spine Orthopedics.Methods:From January 2019 to September 2020, convenience sampling was used to select 160 patients with spreading moxibustion in the Department of Spine Orthopedics of Liuzhou Traditional Chinese Medicine Hospital as the research objects. According to the order of treatment, patients were randomly divided into the control group and the experimental group, with 80 cases in each group. The control group used conventional methods for spreading moxibustion, and the experimental group used self-made molds for spreading moxibustion. The ginger column cracking of the two groups of patients, and operation time of 6 operators for spreading moxibustion on the two groups of patients was compared.Results:The cracking rate of ginger columns in the experimental group was lower than that in the control group, and the difference was statistically significant ( P<0.01) . The operation time of the 6 operators on the patients in the experimental group was (2.5±0.23) min, which was shorter than (18.5±0.41) min in the control group, and the difference was statistically significant ( P<0.01) . Conclusions:The application of self-made molds can shorten the spreading moxibustion operating time of operators and reduce the cracking rate of ginger columns, which is worthy of clinical application.

Objective:To assess the clinical characteristics and grafts status by coronary angiography(CAG) in symptomatic patients with prior coronary artery bypass graft(CABG).Methods:A retrospective descriptive study of symptomatic patients with prior CABG who underwent CAG was performed, 1 136 patients were included and analyzed. The mean age was(62.5±8.7) years, 76.4% were male. There was a high prevalence of risk factors like hypertension(75.0%), dyslipidemia(48.2%), diabetes(46.1%) and smoking history(62.8%).Results:The mean duration after CABG was (4.65±3.39) years. 94.5% of patients had chest pain. 12.9% of patients had all diseased grafts and 28.7% had all patent grafts. The proportion of diseased SVG was higher than that of diseased arterial grafts. The proportion of diseased grafts anastomosed to RCA territory was higher than that of grafts anastomosed to LCX territory or LAD territory. 52.5% of patients received percutaneous coronary intervention(PCI) revascularization, and 88.3% of PCI was performed in native vessels.Conclusion:The most common symptom recurring to patients with prior CABG was chest pain. Graft status in symptomatic patients with prior CABG was worse than we expected. Patients received repeated revascularization mostly by PCI and PCI was mainly performed in native vessels.

Objective:To explore the perioperative effect of coronary artery bypass grafting(CABG) or CABG+ mitral valve repair(MVP) in patients with coronary heart disease(CAD) and moderate ischemic mitral regurgitation(IMR).Methods:The clinical data and perioperative complications of 210 patients with CAD and moderate IMR, who underwent CABG from January 2018 to December 2019, were included into this study, with 155 males and mean age of(62.3±8.5) years old. According to the operation mode, patients were divided into CABG group(138 cases) and CABG+ MVP group(72 cases).Results:There were no significant differences in age, gender, comorbidities(diabetes, hypertension, hyperlipidemia, peripheral vascular disease, cerebrovascular events, previous history of myocardial infarction and PCI), LVEF and of coronary artery lesions between the two groups(all P>0.05). Sequential anastomosis was the main method, and most patients underwent internal mammary artery graft in both groups, there was no significant difference between the two groups( P>0.05). CABG group was higher than CABG+ MVP group in all-cause death, heart failure, cerebrovascular events, secondary thoracotomy, CRRT and IABP support events, but there were no significant differences between the two groups( P>0.05). Echocardiographic reexamination showed that the indexes of cardiac function in CABG+ MVP group were higher than those in CABG group, but there was no significant difference between the two groups( P>0.05). The mean area of mitral regurgitation in CABG + MVP group was 1.3 cm 2, significantly lower than that in CABG group(2.5 cm 2), P<0.05. Conclusion:CABG+ MVP has low perioperative risk in patients with CAD and moderate IMR, and the area of mitral regurgitation is lower.

Objective To analyze clinicopathological characteristics and the potential risk-related factors of female breast hyperplasia in different age groups.Method From Jan 2015 to Dec 2016,patients diagnosed with breast hyperplasia in 12 hospitals were evaluated.All patients completed the self-designed questionnaires on women'health,including basic demographic information,clinic examination information,radiologic information and pathologic results.The patients were divided into a young group (＜ 45 years old) and an elderly group (from 45 to 75 years old).Results There were 3 684 cases of breast hyperplasia,including 2 291 cases in young group and 1 393 cases in elder group,respectively Clinically breast pain type were most commonly observed in both young and older groups (50.3％ vs.42.7％,P ＜ 0.001).While pathological research based on biopsy showed that breast adenopathy were the most common changes in both groups (67.9％ vs.61.7％,P ＜0.001).More breast cancer cases were identified in elder group than that in young group,especially in clinically lump type patients (9.4％ vs.4.2％,P ＜ 0.001).Compared with elder group,patients in young group have different distribution characteristics regarding to fertility factors,lifestyle factors and psychology scale including anxiety and depression.Conclusion Distributions of clinicopathological characteristics and risk factors of female breast hyperplasia differ across different age groups.