[Objective]To evaluate the efficacy of Shengjin Liyan Spray(SJLYS)in alleviating chemotherapy-induced xerostomia in lymphoma patients.[Methods]In this double-blind,randomized,placebo-controlled trial,120 lymphoma patients with chemotherapy-induced xerostomia were enrolled and equally allocated in experimental group(60 cases,receiving SJLYS)and control group(60 cases,receiving sterile water spray).The intervention was administered hourly during chemotherapy until bedtime.Primary outcomes included the numerical rating scale(NRS)for dry mouth,lip mucosal moisture score and unstimulated whole saliva(UWS)flow rate.Secondary outcome was the overall response rate.[Results]Post-treatment assessments demonstrated significantly better outcomes in experimental group,the NRS score of experimental group(2.41±1.10)was significantly lower than that of control group(4.15±1.28);the moisture score of the lip mucosa was(3.47±0.62)points,which was better than control group(2.60±0.78)points;the UWS was(0.53±0.10)mg/min,higher than that of control group(0.42±0.09)mg/min.The overall response rate in experimental group(78.33%)was markedly superior to control group(48.33%),and the above differences were statistically significant(P<0.05).[Conclusion]SJLYS significantly relieves xerostomia symptoms,enhances oral comfort,and optimizes quality of life in chemotherapy-treated lymphoma patients,demonstrating substantial clinical applicability.

Objective To explore the effects of hepatitis B virus genotypes B and C on hepatic stellate cells(HSC)proliferation,cell cycle,and secretion under different alanine aminotransferase(ALT)statuses.Methods A total of 270 patients diagnosed with chronic hepatitis B in the First Hospital of Lanzhou University between January 2023 to December 2023 were selected by stratified random sam-pling.In the group with normal ALT,there were 42 patients with genotype B and 48 patients with genotype C.In the group with ALT ≥ the upper normal limit(UNL)and＜2 times the UNL(2 UNL),there were 48 patients with genotype B and 45 patients with genotype C.In the group with ALT≥2 UNL,there were 45 patients with genotype B and 42 patients with genotype C.Sera from these patients was used as the interfering sera.After HSC were treated with the interfering serum,the proliferation of HSC was detected by the MTT method,the cell cycle was detected by flow cytometry,the mRNA expressions of TGF-β1,Smad3,Smad7,α-SMA,Collagen Ⅰ,and Collagen Ⅲ were detected by real-time PCR,and the expressions of the corresponding proteins were detected by Western blotting.Results When the ALT level was normal,hepatitis B virus genotypes B and C had no obvious effect on HSCs.When the ALT level was abnormal,particularly in the group with ALT ≥ 2 UNL,the virus with genotype C was more likely to promote the proliferation of HSC,reduce the number of cells in the G0/G1 phase,increase the number of cells in the S phase and G2/M phase,enhance the mRNA and protein expressions of TGF-β1,Smad3,Collagen Ⅰ,Collagen Ⅲ,and α-SMA,and decrease the mRNA and protein expressions of Smad7,with significant differences(P＜0.05).Conclusion The effects of hepatitis B virus genotypes B and C on HSC differ varying ALT statuses.During abnormal ALT elevation,genotype C hepatitis B virus demonstrates more pronounced effects on HSC proliferation,cell cycle progression,and secretory function compared to genotype B.

Objective To investigate the effect of hyperglycemia activated CCAAT enhancer binding protein β(C/EBPβ)promoting the expression of thyroid hormone nuclear receptor α1(THα1)on diabetic cardiomyopathy(DCM).Methods Twenty healthy male Wistar rats were randomly divided into diabetes mellitus(DM,n=10)group and DCM(n=10)group.Cardiomyocyte were divided into four groups:DM-cell,glucose intervention(Glu),knockdown C/EBPβ group(cardiomyocyte were transfected with plasmids targeting knockdown C/EBPβ)and si-NC group(cardiomyocyte were transfected with nagetive control sequence of plasmids).HE and Masson staining was performed to detect myocardial injury and collagen deposition.Echocardiography was used to evaluate left ventricular function.The mRNA and protein expression of C/EBP β and THα1 was detected using RT-qPCR,Western blot and immunofluorescence staining in myocardial cells.Apoptosis was analyzed using flow cytometric.Contraction frequency was counted by microscopy.Results Compared to DM group,count of fibroblasts,collagen volume fraction,end-systolic dimension of left ventricular,and the mRNA and protein expression of C/EBPβ and THα1 as well as fluorescence intensity increased(P<0.05);left ventricular ejection fraction and left ventricular fractional shortening(LVFS)decreased in DCM group(P<0.05).The mRNA and protein expression of C/EBPβ and THα1 were higher in the Glu group than in DM-cell group(P<0.05).The percentage of apoptosis,the mRNA and protein expression of THα1 of myocardial cells were lower in the si-C/EBPβ group than in the si-NC group(P<0.05),and LVFS was higher in the si-NC group(P<0.05).Conclusions High glucose regulates C/EBPβ expression,which in turn drives THα1 expression in myocardial tissue,leading to adverse effects on myocardial tissue.Conversely,silencing C/EBPβ downregulates THα1 expression,and exerts a protective effect on cardiomyocytes.

Objective To investigate the effect of hyperglycemia activated CCAAT enhancer binding protein β(C/EBPβ)promoting the expression of thyroid hormone nuclear receptor α1(THα1)on diabetic cardiomyopathy(DCM).Methods Twenty healthy male Wistar rats were randomly divided into diabetes mellitus(DM,n=10)group and DCM(n=10)group.Cardiomyocyte were divided into four groups:DM-cell,glucose intervention(Glu),knockdown C/EBPβ group(cardiomyocyte were transfected with plasmids targeting knockdown C/EBPβ)and si-NC group(cardiomyocyte were transfected with nagetive control sequence of plasmids).HE and Masson staining was performed to detect myocardial injury and collagen deposition.Echocardiography was used to evaluate left ventricular function.The mRNA and protein expression of C/EBP β and THα1 was detected using RT-qPCR,Western blot and immunofluorescence staining in myocardial cells.Apoptosis was analyzed using flow cytometric.Contraction frequency was counted by microscopy.Results Compared to DM group,count of fibroblasts,collagen volume fraction,end-systolic dimension of left ventricular,and the mRNA and protein expression of C/EBPβ and THα1 as well as fluorescence intensity increased(P<0.05);left ventricular ejection fraction and left ventricular fractional shortening(LVFS)decreased in DCM group(P<0.05).The mRNA and protein expression of C/EBPβ and THα1 were higher in the Glu group than in DM-cell group(P<0.05).The percentage of apoptosis,the mRNA and protein expression of THα1 of myocardial cells were lower in the si-C/EBPβ group than in the si-NC group(P<0.05),and LVFS was higher in the si-NC group(P<0.05).Conclusions High glucose regulates C/EBPβ expression,which in turn drives THα1 expression in myocardial tissue,leading to adverse effects on myocardial tissue.Conversely,silencing C/EBPβ downregulates THα1 expression,and exerts a protective effect on cardiomyocytes.

Objective To explore the effects of hepatitis B virus genotypes B and C on hepatic stellate cells(HSC)proliferation,cell cycle,and secretion under different alanine aminotransferase(ALT)statuses.Methods A total of 270 patients diagnosed with chronic hepatitis B in the First Hospital of Lanzhou University between January 2023 to December 2023 were selected by stratified random sam-pling.In the group with normal ALT,there were 42 patients with genotype B and 48 patients with genotype C.In the group with ALT ≥ the upper normal limit(UNL)and＜2 times the UNL(2 UNL),there were 48 patients with genotype B and 45 patients with genotype C.In the group with ALT≥2 UNL,there were 45 patients with genotype B and 42 patients with genotype C.Sera from these patients was used as the interfering sera.After HSC were treated with the interfering serum,the proliferation of HSC was detected by the MTT method,the cell cycle was detected by flow cytometry,the mRNA expressions of TGF-β1,Smad3,Smad7,α-SMA,Collagen Ⅰ,and Collagen Ⅲ were detected by real-time PCR,and the expressions of the corresponding proteins were detected by Western blotting.Results When the ALT level was normal,hepatitis B virus genotypes B and C had no obvious effect on HSCs.When the ALT level was abnormal,particularly in the group with ALT ≥ 2 UNL,the virus with genotype C was more likely to promote the proliferation of HSC,reduce the number of cells in the G0/G1 phase,increase the number of cells in the S phase and G2/M phase,enhance the mRNA and protein expressions of TGF-β1,Smad3,Collagen Ⅰ,Collagen Ⅲ,and α-SMA,and decrease the mRNA and protein expressions of Smad7,with significant differences(P＜0.05).Conclusion The effects of hepatitis B virus genotypes B and C on HSC differ varying ALT statuses.During abnormal ALT elevation,genotype C hepatitis B virus demonstrates more pronounced effects on HSC proliferation,cell cycle progression,and secretory function compared to genotype B.

[Objective]To evaluate the efficacy of Shengjin Liyan Spray(SJLYS)in alleviating chemotherapy-induced xerostomia in lymphoma patients.[Methods]In this double-blind,randomized,placebo-controlled trial,120 lymphoma patients with chemotherapy-induced xerostomia were enrolled and equally allocated in experimental group(60 cases,receiving SJLYS)and control group(60 cases,receiving sterile water spray).The intervention was administered hourly during chemotherapy until bedtime.Primary outcomes included the numerical rating scale(NRS)for dry mouth,lip mucosal moisture score and unstimulated whole saliva(UWS)flow rate.Secondary outcome was the overall response rate.[Results]Post-treatment assessments demonstrated significantly better outcomes in experimental group,the NRS score of experimental group(2.41±1.10)was significantly lower than that of control group(4.15±1.28);the moisture score of the lip mucosa was(3.47±0.62)points,which was better than control group(2.60±0.78)points;the UWS was(0.53±0.10)mg/min,higher than that of control group(0.42±0.09)mg/min.The overall response rate in experimental group(78.33%)was markedly superior to control group(48.33%),and the above differences were statistically significant(P<0.05).[Conclusion]SJLYS significantly relieves xerostomia symptoms,enhances oral comfort,and optimizes quality of life in chemotherapy-treated lymphoma patients,demonstrating substantial clinical applicability.

[Objective]People infected with Hepatitis B are often divided into hepatitis B carriers and hepatitis B patients based on whether ALT is normal or not,and ALT ≥ 2UNL is one of the indications for clinical antiviral treatment,but no sufficient evidence to justify this. In order to explore the theoretical basis,the paper investigated the effects of hepatitis B virus(HBV) on hepatic stellate cells(HSCs).[Methods]A total of 132 chronic hepatitis B patients with different viral loads and ALT levels were randomly selected as the study subjects. Of these patients,those with ALT≥2UNL were treated with antiviral therapy and followed up for 24 weeks. The effects of HBV on HSCs before and after the treatment were compared and analyzed. HSCs proliferation was detected by MTT method,HSCs cell cycle by flow cytometry,and expression of TGF-β1,Smad3,Smad7,α-SMA,collagen Ⅰ,collgen Ⅲ mRNAs and corresponding proteins by RT-PCR and Western blotting,respectively.[Results]At the normal ALT level,HBV with different viral loads had no significant effect on the proliferation,cell cycle and cell secretion of the HSCs. At the abnormal ALT level,especially when ALT ≥ 2UNL,with the increase of virus loads,HSCs proliferation accelerated;cells in the G0/G1 phase decreased significantly and cells in the S and G2/M phases increased significantly;the expression of TGF-β1,Smad3,α-SMA,collagen Ⅰ,collgen Ⅲ mRNAs and corresponding proteins increased significantly,but Smad7 mRNA and protein expression decreased significantly,the differences were statistically significant. HBV showed a significantly lower effect on HSCs after the antiviral therapy than before.[Conclusions]This paper reveals the differential effects of HBV on HSCs at different ALT levels and presents a comparative analysis of the effects before and after the antiviral therapy,which provides a theroretical basis for identifying the ALT level as an indication for HBV antiviral therapy.

Objective To analyze the screening results and risk assessment of common malignant tumors among female residents in Deyang from 2017 to 2019, so as to provide basis for cancer prevention and control. Methods Women who underwent physical examinations and completed the survey of common malignant tumors related risk factors from 2017 to 2019 were selected. The high risk rate, screening rate and detection rate of common malignant tumor among female urban residents in Deyang were analyzed. Results From 2017 to 2019, a total of 49,871 female urban residents completed the survey of common malignant tumor related risk factors. The risk rates of breast cancer, lung cancer, cervical cancer and colorectal cancer were 13.24%, 7.65%, 12.92% and 5.53%, respectively. Among all the age groups, women at the age of 50~59 years old were at the highest risk of breast cancer, lung cancer, cervical cancer, colorectal cancer; The screening rates of breast cancer, lung cancer, cervical cancer and colorectal cancer were 24.68%, 11.72%, 34.62% and 14.15%, respectively; The screening rates of breast cancer, lung cancer, cervical cancer and colorectal cancer among women with different educational levels showed significant difference (P<0.05). The screen rates of breast cancer, lung cancer, cervical cancer and colorectal cancer among high risk groups were 24.68%, 11.75%, 26.56% and 14.35%, respectively; The detection rates of breast cancer, lung cancer, cervical cancer and colorectal cancer among the screening groups were 9.33%, 2.68%, 9.82%, and 2.02%, respectively (P<0.05). Conclusion Female residents in Deyang from 2017 to 2019 have high risk and detection rates of breast cancer and cervical cancer, so active screening of malignant tumors among urban female residents can effectively improve the residents' quality of life, and is of vital importance for the early diagnosis and treatment of malignant tumors.