Objective To investigate the relationship between the expression levels of growth differentia-tion factor 15(GDF-15)and signal transduction molecule 3(SMAD3)in the serum of patients with acute cer-ebral hemorrhage and neurological dysfunction as well as prognosis.Methods A total of 109 patients with a-cute cerebral hemorrhage who were treated at the Gong'an County People's Hospital from June 2018 to June 2023 were selected as the research group.The National Institutes of Health Stroke Scale(NIHSS)score was used to assess the degree of neurological impairment in patients who were grouped into mild injury group(n=44),moderate injury group(n=37),and severe injury group(n=28).The modified Rankin scale was used to evaluate the prognosis of the patients,and they were divided into the good prognosis group(m=78)and the poor prognosis group(n=31).Another 80 healthy individuals who underwent physical examinations during the same period were selected as the control group.The serum GDF-15 and SMAD3 levels of each group were Detected and compared.The Spearman method was used to analyze the correlation between the levels of serum GDF-15 and SMAD3 and the degree of neurological function impairment.Logistic regression was used to ana-lyze the factors influencing the prognosis of patients.The receiver operating characteristic(ROC)curve was used to evaluate the predictive efficacy of serum GDF-15 and SMAD3 levels for poor prognosis.Results Com-pared with the control group,the levels of serum GDF-15 and SMAD3 in the research group were significantly increased(P＜0.05).With the aggravation of the severity of neurological function impairment in patients with acute cerebral hemorrhage,the levels of serum GDF-15 and SMAD3 further increased(P＜0.05).The a-nalysis results of the Spearman method showed that the levels of serum GDF-15 and SMAD3 were positively correlated with the severity of neurological function impairment(P＜0.05).The levels of serum GDF-15 and SMAD3 in the poor prognosis group were significantly higher than those in the good prognosis group(P＜0.05).The cerebral hemorrhage volume,NIHSS score,proportion of hypertension and proportion of alcohol consumption history in the poor prognosis group were all higher than those in the good prognosis group(P＜0.05).Logistic regression analysis showed that elevated cerebral hemorrhage volume,NIHSS score,serum GDF-15 and SMAD3 levels were risk factors affecting the poor prognosis of patients(P＜0.05).The ROC curve analysis results indicated that the levels of serum GDF-15 and SMAD3 could predict the occurrence of poor prognosis in patients,and the combined detection of the two could improve the clinical predictive effica-cy.Conclusion The levels of serum GDF-15 and SMAD3 are closely related to neurological dysfunction and prognosis in patients with acute cerebral hemorrhage.The combination of the two has a high predictive value for the prognosis of patients with acute cerebral hemorrhage.

OBJECTIVETo investigate the effects of Hydroxy Safflor yellow A (HSYA) on the growth of blood vessel of transplantation tumor of gastric adenocarcinoma cell line BGC-823 in nude mice and its underlying mechanism of antagonizing tumor angiogenesis.

METHODThe BGC-823 cells was subcutaneouly injected into the right anterior armpit of BALB/C nu/nu nude mice and established the animal model of transplantation tumor. Then nude mice were divided into 4 groups at random: model group, control group, high or low dosage of HSYA group. The model group were treated with normal sodium by intraperitoneal injection, HSYA groups were treated with HSYA at concentration of 0.056 g x L(-1) and 0.028 g x L(-1) by intraperitoneal injection, and in these groups each mouse was injected 2 times everyday with 0.2 mL by 4-6 hours interval. The control group were injected in enterocoelia 1 times every 2 days starting from the third day with cytoxan at 2 g x L(-1). 20 days later, the volume and weight of nude mice were observed. The pathological change of tumor tissue was observed under optical microscope. The mRNA expression of VEGF and bFGF of transplantation tumor were detected by real time quantitative PCR.

RESULTThe volume (607.42 +/- 252.96) mm3, weight (0.88 +/- 0.14) g of transplantation tumor, the mRNA expression level of VEGF 0.49 +/- 0.13 and bFGF 0.60 +/- 0.48 are reduced significantly after treatment with HSYA at the concentration of 0.028 g x L(-1) compared with physiologic saline-treated group (P < 0.05 or P < 0.01). The tumor pathological angiogenesis of HSYA group is also less obvious than the normal sodium-treated group.

CONCLUSIONHSYA in given concentration can inhibit the growth of BGC-823 transplantation tumor, and decreasing the mRNA expression of VEGF and bFGF, which suggests that inhibiting tumor angiogenesis may be one of the mechanisms of HSYA antagonizing tumor.

Adenocarcinoma ; drug therapy ; genetics ; metabolism ; Angiogenesis Inhibitors ; administration & dosage ; Animals ; Blood Vessels ; drug effects ; Cell Line, Tumor ; Chalcone ; administration & dosage ; analogs & derivatives ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Fibroblast Growth Factors ; genetics ; metabolism ; Gene Expression Regulation, Neoplastic ; drug effects ; Humans ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; Quinones ; administration & dosage ; Random Allocation ; Stomach Neoplasms ; drug therapy ; genetics ; metabolism ; Vascular Endothelial Growth Factor A ; genetics ; metabolism