Objective:To analyze the characteristics of acute chemical poisoning cases and provide data support for establishing a regional network poisoning early warning and surveillance platform.Methods:In July 2023, patients with acute chemical poisoning who visited the Emergency Department, West China School of Public Health, and West China Fourth Hospital, Sichuan University from May 2016 to May 2023 were selected as the research subjects. Their characteristics such as gender, age, types of poisons, seasonal distribution, and the change trend of the number of poisoned people were analyzed. The annual trend of poisoning population was analyzed by linear regression.Results:There were a total of 2033 patients with acute chemical poisoning, among whom 32 were children (accounting for 1.57%). The gender was mainly female (1146 cases, accounting for 56.37%). The age ranged from 4 to 86 (34.60±10.45) years old. The top three poisonings in terms of acute chemical poisoning were carbon monoxide poisoning with 889 cases (accounting for 43.73%), diquat poisoning with 193 cases (accounting for 9.49%), and paraquat poisoning with 168 cases (accounting for 8.26%). Sedative-hypnotic drug poisoning was mainly in spring (65 cases, accounting for 43.62%), paraquat (65 cases, accounting for 38.69%) and diquat (68 cases, accounting for 35.23%) poisoning was mainly in summer, and carbon monoxide poisoning was mainly in winter (452 cases, accounting for 50.84%). The number of poisoning cases showed a significant increasing trend over the past seven years ( t=6.97, P<0.001). The detection rate of carbon monoxide poisoning was 95.61% (850/889), the detection rate of diquat poisoning was 28.50% (55/193), and the detection rate of paraquat poisoning was 14.88% (25/168) . Conclusion:The poisons of acute chemical poisoning are mainly carbon monoxide, diquat and paraquat. It is necessary to strengthen the publicity and education related to chemical poisoning, the supervision of poisons, and the construction of regional network poisoning early warning and surveillance platforms.

Healthy dental pulp is essential for preserving teeth and maintaining their normal function. Vital pulp therapy (VPT) is widely used in clinical applications because it aims to preserve vital pulp and enhance the long-term survival of teeth. An accurate diagnosis of pulp vitality is a prerequisite for successful VPT. However, accurately assessing pulp viability remains challenging in clinical practice. Pulp viability is influenced by various factors, including the type of pulp exposure, caries status, periodontitis, trauma, treatment factors, patient age, and individual differences. Assessing pulp viability requires a comprehensive consideration of medical history and clinical manifestations, along with a combination of various auxiliary methods, such as pulp sensibility tests, pulp blood flow tests, imaging techniques and molecular diagnostics. In the future, the technology for assessing pulp vitality should evolve toward chairside, visualization, and precision techniques, to achieve consistency between clinical and histological diagnoses, thereby providing patients with the most effective treatment.

Objective:To analyze the characteristics of acute chemical poisoning cases and provide data support for establishing a regional network poisoning early warning and surveillance platform.Methods:In July 2023, patients with acute chemical poisoning who visited the Emergency Department, West China School of Public Health, and West China Fourth Hospital, Sichuan University from May 2016 to May 2023 were selected as the research subjects. Their characteristics such as gender, age, types of poisons, seasonal distribution, and the change trend of the number of poisoned people were analyzed. The annual trend of poisoning population was analyzed by linear regression.Results:There were a total of 2033 patients with acute chemical poisoning, among whom 32 were children (accounting for 1.57%). The gender was mainly female (1146 cases, accounting for 56.37%). The age ranged from 4 to 86 (34.60±10.45) years old. The top three poisonings in terms of acute chemical poisoning were carbon monoxide poisoning with 889 cases (accounting for 43.73%), diquat poisoning with 193 cases (accounting for 9.49%), and paraquat poisoning with 168 cases (accounting for 8.26%). Sedative-hypnotic drug poisoning was mainly in spring (65 cases, accounting for 43.62%), paraquat (65 cases, accounting for 38.69%) and diquat (68 cases, accounting for 35.23%) poisoning was mainly in summer, and carbon monoxide poisoning was mainly in winter (452 cases, accounting for 50.84%). The number of poisoning cases showed a significant increasing trend over the past seven years ( t=6.97, P<0.001). The detection rate of carbon monoxide poisoning was 95.61% (850/889), the detection rate of diquat poisoning was 28.50% (55/193), and the detection rate of paraquat poisoning was 14.88% (25/168) . Conclusion:The poisons of acute chemical poisoning are mainly carbon monoxide, diquat and paraquat. It is necessary to strengthen the publicity and education related to chemical poisoning, the supervision of poisons, and the construction of regional network poisoning early warning and surveillance platforms.

Objective To evaluate the postoperative analgesia efficacy and clinical safety of hydro-morphone patients-controlled intravenous analgesia(PCIA)in patients with scar pregnancy after auxiliary uterine artery embolization(UAE).Methods A total of 116 patients with scar pregnancy,who received auxiliary UAE at the Fuyang Municipal People's Hospital of China between January 2021 and September 2022,were enrolled in this study.According to the intravenous self-controlled analgesic drugs used after UAE,the patients were randomly and equally divided into observation group(n=58)and control group(n=58).Ten minutes before the procedure,intravenous injection of 2 mg hydromorphone(observation group)or 2 μg/kg sufentanyl(control group)was performed,and the PCIA pump was connected.In the observation group,the mixed solution of 10 mg hydromorphone+100 mg flurbiprofen axetil+100 mL saline was put in the analgesic pump,while in the control group,the mixed solution of 2 μg/kg sufentanyl+flurbiprofen axetil 100 mg+100 mL saline was put in the analgesic pump.The post-UAE 0.5-h,4-h,8-h,12-h,24-h and 48-h visual analogue scale(VAS)scores,the Bruggrmann comfort scale(BCS)scores,the number of pressing analgesic pump times within postoperative 48 hours,the used dosage of analgesic drugs,the adverse reactions,and the incidence of postoperative complications were recorded.Results The difference in the post-UAE 0.5-h VAS scores between the observation group and the control group was not statistically significant(P＞0.05),while the post-UAE 4-h,8-h,12-h,24-h and 48-h VAS scores in the observation group were significantly lower than those in the control group,and the differences were statistically significant(all P＜0.05).The post-UAE 0.5-h,4-h,8-h,12-h,24-h and 48-h BCS scores in the observation group were significantly higher than those in the control group,and the differences were statistically significant(all P＜0.05).The number of pressing analgesic pump times and the used dosage of analgesic drugs within postoperative 48 hours in the observation group were lower than those in the control group,and the differences were statistically significant(all P＜0.05).No statistically significant differences in the complications such as drowsiness,skin itching,hypoxia,or respiratory depression,etc.existed between the two groups,while the difference in the incidence of adverse reactions between the two groups was statistically significant(P＜0.05).Conclusion Hydromorphone and sufentanil PCIA can relieve the pain in scar pregnancy patients after UAE.Hydromorphone is superior to sufentanil in reducing the number of pressing analgesic pump times within postoperative 48 hours,reducing the used dosage of analgesic drugs,and decreasing the incidence of adverse reactions,therefore,hydromorphone PCIA has a certain promotion value.(J Intervent Radiol,2024,33:240-244)

Objective To compare the effect of internal iliac artery block using balloon of different properties in cesarean section for delivery female patients with pernicious placenta previa(PPP).Methods The clinical data of 82 delivery female patients with PPP were retrospectively analyzed.The patients were divided into observation group(n=40)and control group(n=42).The patients of the observation group received compliant Fogarty balloon block of bilateral internal iliac arteries,while the patients of the control group received non-compliant balloon block of bilateral internal iliac arteries.The preoperative and postoperative systolic blood pressure and heart rate,X-ray fluoroscopy time,fetal body surface radiation dose,time spent for cesarean section,intraoperative blood loss amount and blood transfusion quantity,uterine artery embolization rate,hysterectomy rate,1-,5-,and 10-minute neonatal Apgar scores,postoperative hospital stay,and surgery-related complications were compared between the two groups.Results In all patients of both groups,the uterus was successfully preserved and the bilateral internal iliac artery balloon obstruction-assisted cesarean section was successfully accomplished.After the balloon catheter was withdrawn,uterine artery embolization was carried out in some patients,and no patient received ovarian artery embolization and hysterectomy.No statistically significant differences in preoperative,postoperative systolic blood pressure and heart rate existed between the two groups,but in both groups the postoperative systolic blood pressure and heart rate were lower than their preoperative values(all P＜0.05).No statistically significant differences in X-ray fluoroscopy time,fetal body surface radiation dose,uterine artery embolization rate,hysterectomy rate,1-,5-,and 10-minute neonatal Apgar scores,and postoperative hospital stay existed between the two groups.In the observation group the time spent for cesarean section,intraoperative blood loss amount and blood transfusion quantity were lower than those in the control group(all P＜0.05).The difference in the incidence of balloon obstruction-related complications between the two groups was not statistically significant.Conclusion In cesarean section for delivery female patients with PPP,balloon block of bilateral internal iliac,regardless of using compliant Fogarty balloon catheter or non-compliant balloon catheter,is clinically safe and effective.However,the use of compliant Fogarty balloon catheter is superior to the use of non-compliant balloon catheter in reducing the cesarean section operative time,intraoperative blood loss amount,and intraoperative blood transfusion quantity.

Objective To construct and apply an intelligent follow-up information system for patients with cancer pain,providing references for improving the efficiency of hospital follow-up and promoting pain management of patients at home.Methods The intelligent discharge follow-up system for patients with cancer pain includes 2 platforms,namely a patient self-report platform and an administrator operation platform.The administrator operation platform consists of 5 modules,namely the workbench module,the follow-up plan module,the follow-up results module,the health education module and the data statistics module.In January 2022,the system was officially put into clinical application.The use of the system was analyzed,and patients'completion rate,medication compliance,incidence of moderate and severe pain and satisfaction with pain control were compared before(from January 2020 to December 2021)and after(from January 2022 to November 2023)the application of the system.Results At present,this system has been applied in 95 cancer-related wards of our hospital.From January 2022 to November 2023,the number of people who should be followed up was 4 248,and the number of people who actually completed the follow-up was 4 127;the rate of follow-up completion was 97.2%;the rate of timely completion of the follow-up was 94.9%;the rate of automatic follow-up by the system was 40.1%;the rate of patient abnormality report was 31.9%;the rate of timely treatment of patient abnormality report was 89.1%.After the application of the system,the completion rate of pain follow-up was increased,and the difference was statistically significant(P<0.001).After the application of the system,the medication compliance rate of patients with cancer pain increased from 86.9%to 91.0%;the incidence of moderate and severe pain decreased from 6.8%to 5.2%;the satisfaction with pain control increased from 81.0%to 83.5%(P<0.05).Conclusion The intelligent discharge follow-up system for patients with cancer pain can effectively improve the discharge follow-up efficiency and promote the management of patients with cancer pain at home.

Objective To explore the establishment of a subcutaneously transplanted tumor model of hepatocellular carcinoma in mice with Qi stagnation and blood stasis syndrome.Methods Forty male C57BL/6 mice were randomly divided into 4 groups:NC group,QZXY group,Tumor group,and QZXY+Tumor group.They were categorized based on the modeling of Qi stagnation and blood stasis syndrome(7 days)combined with the modeling of subcutaneous transplantation of hepatocellular carcinoma tumor(20 days).Observations were conducted of the syndrome manifestations as well as the tumor size and weight of the mice after modeling.Results(1)Body weight:on the 7th day of modeling,the weights of the QZXY group and QZXY+Tumor group were significantly lower than that of the NC group(P＜0.05).(2)Body temperature:on the 7th day of modeling,body temperature significantly decreased in the QZXY group(P＜0.05),while it increased in the Tumor group(P＜0.05)compared with the NC group.On the 27th day of modeling,the temperature of the QZXY+Tumor group was significantly lower than that of the NC group(P＜0.05).(3)Syndrome manifestations:according to the syndrome scoring table,mice in both the QZXY group and QZXY+Tumor group exhibited Qi stagnation and blood stasis syndrome on the 7th day of modeling(P＜0.05).As modeling time extended,the score of mice in the Tumor group increased with the formation of the tumor,and the score of mice in the QZXY+Tumor group was significantly higher than that of the other three groups(P＜0.05).(4)Claw petechiae:the number of claw petechiae significantly increased in all three groups of modeled mice compared with the NC group(P＜0.05),with the QZXY+Tumor group showing the highest number.(5)Claw r value:the r value of the claw was significantly lower in all three groups of modeled mice than that in the NC group(P＜0.05).Additionally,the r value of the claw in the QZXY+Tumor group was consistently lower than that of the other three groups.(6)Open field activity:the vertical and horizontal activity of mice in the QZXY+Tumor group decreased significantly compared with that of the NC group(P＜0.05).(7)Coagulation indexes:APTT,TT,and FIB were significantly increased in the QZXY+Tumor group(P＜0.05 or P＜0.01)compared with those in the NC group.(8)Tumor size and weight:compared with the Tumor group,the QZXY+Tumor group showed significantly increased tumor size and weight(P＜0.05).Conclusions This study successfully established a subcutaneous transplanted tumor model of hepatocellular carcinoma in mice with Qi stagnation and blood stasis syndrome.The findings indicated that Qi stagnation and blood statsis syndrome may occur during the course of live cancer.Besides,the causes inducing the Qi stagnation and blood stasis syndrome will further accelerate the progression of liver cancer.

Objective To explore the establishment of a subcutaneously transplanted tumor model of hepatocellular carcinoma in mice with Qi stagnation and blood stasis syndrome.Methods Forty male C57BL/6 mice were randomly divided into 4 groups:NC group,QZXY group,Tumor group,and QZXY+Tumor group.They were categorized based on the modeling of Qi stagnation and blood stasis syndrome(7 days)combined with the modeling of subcutaneous transplantation of hepatocellular carcinoma tumor(20 days).Observations were conducted of the syndrome manifestations as well as the tumor size and weight of the mice after modeling.Results(1)Body weight:on the 7th day of modeling,the weights of the QZXY group and QZXY+Tumor group were significantly lower than that of the NC group(P＜0.05).(2)Body temperature:on the 7th day of modeling,body temperature significantly decreased in the QZXY group(P＜0.05),while it increased in the Tumor group(P＜0.05)compared with the NC group.On the 27th day of modeling,the temperature of the QZXY+Tumor group was significantly lower than that of the NC group(P＜0.05).(3)Syndrome manifestations:according to the syndrome scoring table,mice in both the QZXY group and QZXY+Tumor group exhibited Qi stagnation and blood stasis syndrome on the 7th day of modeling(P＜0.05).As modeling time extended,the score of mice in the Tumor group increased with the formation of the tumor,and the score of mice in the QZXY+Tumor group was significantly higher than that of the other three groups(P＜0.05).(4)Claw petechiae:the number of claw petechiae significantly increased in all three groups of modeled mice compared with the NC group(P＜0.05),with the QZXY+Tumor group showing the highest number.(5)Claw r value:the r value of the claw was significantly lower in all three groups of modeled mice than that in the NC group(P＜0.05).Additionally,the r value of the claw in the QZXY+Tumor group was consistently lower than that of the other three groups.(6)Open field activity:the vertical and horizontal activity of mice in the QZXY+Tumor group decreased significantly compared with that of the NC group(P＜0.05).(7)Coagulation indexes:APTT,TT,and FIB were significantly increased in the QZXY+Tumor group(P＜0.05 or P＜0.01)compared with those in the NC group.(8)Tumor size and weight:compared with the Tumor group,the QZXY+Tumor group showed significantly increased tumor size and weight(P＜0.05).Conclusions This study successfully established a subcutaneous transplanted tumor model of hepatocellular carcinoma in mice with Qi stagnation and blood stasis syndrome.The findings indicated that Qi stagnation and blood statsis syndrome may occur during the course of live cancer.Besides,the causes inducing the Qi stagnation and blood stasis syndrome will further accelerate the progression of liver cancer.

Medical imaging is an important auxiliary means of disease diagnosis. In the process of diagnosis, diseases are usually presented in a static way, which is one of the characteristics of imaging diagnosis. However, this static representation of diseases imposes certain limitations, which can, to some extent, lead to the loss of medical humanities. Therefore, it is very important for imaging diagnostic doctors to correctly use philosophical thinking to understand, analyze, and solve problems. The reinforcement of philosophical thinking in imaging diagnosis entails commitment to the "patient-centered" perspective in medicine as well as the integration of comprehensiveness, regularity, and proactivity. This not only effectively trains the diagnostic thinking of imaging diagnostic doctors but also improves their humanistic quality, thereby addressing the problems associated with these limitations. This allows the diagnosis to be both technically accurate and emotionally attuned.

Cutinase can degrade aliphatic and aromatic polyesters, as well as polyethylene terephthalate. Lack of commercially available cutinase calls for development of cost-effective production of efficient cutinase. In this study, eight cutinase genes were cloned from Sclerotinia sclerotiorum. The most active gene SsCut-52 was obtained by PCR combined with RT-PCR, expressed in Escherichia coli BL21 and purified by Ni-NTA affinity chromatography to study its characteristics and pathogenicity. Sscut-52 had a total length of 768 bp and 17 signal peptides at the N terminals. Phylogenetic analysis showed that its amino acid sequence had the highest homology with Botrytis keratinase cutinase and was closely related to Rutstroemia cutinase. Sscut-52 was highly expressed during the process of infecting plants by Sclerotinia sclerotiorum. Moreover, the expression level of Sscut-52 was higher than those of other cutinase genes in the process of sclerotia formation from mycelium. The heterologously expressed cutinase existed in the form of inclusion body. The renatured SsCut-52 was active at pH 4.0-10.0, and mostly active at pH 6.0, with a specific activity of 3.45 U/mg achieved. The optimum temperature of SsCut-52 was 20-30 ℃, and less than 60% of the activity could be retained at temperatures higher than 50 ℃. Plant leaf infection showed that SsCut-52 may promote the infection of Banlangen leaves by Sclerotinia sclerotiorum.
Ascomycota/genetics* ; Carboxylic Ester Hydrolases ; Cloning, Molecular ; Phylogeny