Objectives:The purpose of this study was to observe the effect of closed-suction irrigation in patients with brucellosis spondylitis(BS)after one-stage posterior debridement and bone graft fusion with inter-nal fixation.Methods:30 patients with BS after one-stage posterior debridement and bone graft fusion with internal fixation,admitted between August 2022 and December 2023,were randomly divided into control group(15 cases)and observation group(15 cases).In the control group,there were 10 males and 5 females,with an age of 44.1±7.8 years;In the observation group,there were 11 males and 4 females,with an age of 42.1±6.6 years.The control group received conventional treatment after operation,and the observation group received gentamicin closed-suction irrigation treatment on the basis of the control group.From the first day after surgery,gentamicin 80mg+normal saline 3000mL was used to continuously inrigate the wound for 2 weeks.The erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),visual analogue scale(VAS),and Os-westry disability index(ODI)were observed before operaion,and on the 1st day after operation,at 1 week,2 weeks,3 months,6 months,and 1 year after operation.The imaging data of the two groups before operation and at 1 year after operation were compared,including lumbar lordosis(LL)and disc height(DH).At 1 year after operation,the fusion rate was evaluated according to Suk classification.The postoperative complications were recorded.Results:On the first day after operation,the levels of ESR and CRP in both groups were significantly higher than those before operation(P＜0.05).At 1 week after operation,the CRP level of patients in the two groups was significantly lower than that before operation(P＜0.05).At 2 weeks after operation,the ESR of the patients in the observation group was lower than that before operation(P＜0.05),while the ESR in the control group was lower than that before operation at 3 months after operation(P＜0.05).With the passage of time,the VAS score and ODI of the two groups were significantly lower than those before operation(P＜0.05).The ESR,CRP,VAS and ODI in the observation group were significantly lower than those in the control group at 1 week,2 weeks and 3 months after operation(P＜0.05).At 6 months after operation,the ESR and CRP levels of patients in both groups returned to normal.One year after operation,both LL and DH in the two groups of patients increased compared with those before operation(P＜0.05).The overall fusion rate was 100％in the control group and 93.33％in the experimental group(P＞0.05).No postoperative complications occurred during the study in either group.Conclusions:Closed-suction irrigation after one-stage posterior debridement and bone-graft fusion with internal fixation for the patients with BS can help to reduce the inflammatory markers,relieve back pain and improve lumbar function.

Objectives:The purpose of this study was to observe the effect of closed-suction irrigation in patients with brucellosis spondylitis(BS)after one-stage posterior debridement and bone graft fusion with inter-nal fixation.Methods:30 patients with BS after one-stage posterior debridement and bone graft fusion with internal fixation,admitted between August 2022 and December 2023,were randomly divided into control group(15 cases)and observation group(15 cases).In the control group,there were 10 males and 5 females,with an age of 44.1±7.8 years;In the observation group,there were 11 males and 4 females,with an age of 42.1±6.6 years.The control group received conventional treatment after operation,and the observation group received gentamicin closed-suction irrigation treatment on the basis of the control group.From the first day after surgery,gentamicin 80mg+normal saline 3000mL was used to continuously inrigate the wound for 2 weeks.The erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),visual analogue scale(VAS),and Os-westry disability index(ODI)were observed before operaion,and on the 1st day after operation,at 1 week,2 weeks,3 months,6 months,and 1 year after operation.The imaging data of the two groups before operation and at 1 year after operation were compared,including lumbar lordosis(LL)and disc height(DH).At 1 year after operation,the fusion rate was evaluated according to Suk classification.The postoperative complications were recorded.Results:On the first day after operation,the levels of ESR and CRP in both groups were significantly higher than those before operation(P＜0.05).At 1 week after operation,the CRP level of patients in the two groups was significantly lower than that before operation(P＜0.05).At 2 weeks after operation,the ESR of the patients in the observation group was lower than that before operation(P＜0.05),while the ESR in the control group was lower than that before operation at 3 months after operation(P＜0.05).With the passage of time,the VAS score and ODI of the two groups were significantly lower than those before operation(P＜0.05).The ESR,CRP,VAS and ODI in the observation group were significantly lower than those in the control group at 1 week,2 weeks and 3 months after operation(P＜0.05).At 6 months after operation,the ESR and CRP levels of patients in both groups returned to normal.One year after operation,both LL and DH in the two groups of patients increased compared with those before operation(P＜0.05).The overall fusion rate was 100％in the control group and 93.33％in the experimental group(P＞0.05).No postoperative complications occurred during the study in either group.Conclusions:Closed-suction irrigation after one-stage posterior debridement and bone-graft fusion with internal fixation for the patients with BS can help to reduce the inflammatory markers,relieve back pain and improve lumbar function.

Objective Previous study found TGF-βpathway might be the molecular pathway influencing the prognosis of colo-rectal cancer, while it was uncertain whether Chinese population is associated with the disease.The article was to evaluate the genetic factors associated with prognosis in colorectal cancer. Methods 52 cases patients with colorectal cancer were followed-up for 36 months in our hospitals from January 2013 to August 2014.Their DNAs were extracted and stored and gene typing were carried out in 5 candidate genes to detect the association between SNPs and the prognosis in colorectal cancer. Results The results showed that within the TGF-βsignaling pathway, after adjusting for Bonferroni multiple testing, allele A of SNP rs10749971 located in gene POU2AF1 was associated with the recurrence of patients with stage III disease under additive and recessive genetic models ( HR =1.968, P=0.004;HR=2.174, P=0.010).Allele C of SNP rs961253 in the gene BMP2 could increase the recurrence risk (HR=1.992, P=0.005) and the death risk (HR=3.161, P=0.007) of patients with stage III disease under recessive genetic models.Allele A of SNP rs4464148 in SMAD7 gene could significantly decrease the death risk of patients with stage II and III colorectal cancer under dominant genetic model (HR=0.382, P=0.017;HR=0.230, P=0.006).In addition, accumulated effects of several adverse genes showed gene high risk group could increase the risk of death for patients with stage III colorectal cancer significantly ( HR=15.512, P=0.036;95%CI:1.611-149.360). Conclusion In different genetic models, SNP locus mutation within gene POU2AF1, BMP2 and SMAD7 on TGF-βpathway was associated with the prognosis of patients with colorectal cancer.With the increase of the number of unfavorable genes, the death risk increases accordingly.