Objective To investigate the effects and mechanisms of suppressor of cytokine signaling 3(SOCS3)overexpression on CD4+T cells imbalance in rats with experimental autoimmune myocarditis(EAM).Methods Ten male Lewis rats were randomly select-ed as the control group from 30male Lewis rats.The remaining 20 rats were injected with porcine cardiac myosin on the 0th and 7th days of the experiment to establish the EAM rat model.These 20 rats were then randomly divided into two groups:the EAM group and the EAM+SOCS3group.The EAM+SOCS3group was injected with SOCS3-overexpressing lentivirus via the tail vein,while the EAM group was injected with an equal volume of empty vector lentivirus.All rats underwent echocardiography to evaluate cardiac function and were then euthanized on the 21st day of the experiment.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of serum cardiac troponin T,creatine kinase-myocardial band,B-type natriuretic peptide,and cytokine levels.Myocardial tissue inflam-mation infiltration and myocardial pathological scores were evaluated by hematoxylin-eosin(HE)staining.The levels of CD4+T cell subsets in the rat spleen were detected by flow cytometry.The expressions of SOCS3,Janus kinase 2(JAK2),signal transducer and acti-vator of transcription 3(STAT3),and CD4+T cell-specific transcription factors were detected by real time fluorescent quantitative poly-merase chain reaction(RT-qPCR)and Western blot.Results In the EAM group,the levels of serum of cardiac troponin T,creatine kinase-myocardial band,and B-type natriuretic peptide,were significantly increased compared with those in the control group(P＜0.05),and the myocardial pathological score was significantly increased(P＜0.05);In the EAM group,the mRNA and protein expres-sions of T-box expressed in T cells(T-bet)and retinoid-related orphan receptor γt(RORγt)in myocardial tissues were significantly increased compared with those in the control group(all P＜0.05),while the expression of forkhead box P3(Foxp3)was significantly de-creased(P＜0.05);In the EAM group,the percentages of helper T cell 1(Th1)and helper T cell 17(Th17)in the spleen were signif-icantly increased compared with those in the control group(both P＜0.05),and the percentage of regulatory T cell(Treg)was signifi-cantly decreased(P＜0.05);In the EAM group,the mRNA and protein expressions of JAK2 and STAT3 in myocardial tissues were signifi-cantly increased compared with those in the control group(P＜0.05).These indexes in the EAM+SOCS3group were significantly restored compared with those in the EAM group(P＜0.05).Conclusion SOCS3 overexpression can ameliorate the imbalance of CD4+T cells and alleviate myocardial damage in EAM rats,and its mechanism is related to the downregulation of the JAK2/STAT3signaling pathway.

Objective To investigate the effects and mechanisms of suppressor of cytokine signaling 3(SOCS3)overexpression on CD4+T cells imbalance in rats with experimental autoimmune myocarditis(EAM).Methods Ten male Lewis rats were randomly select-ed as the control group from 30male Lewis rats.The remaining 20 rats were injected with porcine cardiac myosin on the 0th and 7th days of the experiment to establish the EAM rat model.These 20 rats were then randomly divided into two groups:the EAM group and the EAM+SOCS3group.The EAM+SOCS3group was injected with SOCS3-overexpressing lentivirus via the tail vein,while the EAM group was injected with an equal volume of empty vector lentivirus.All rats underwent echocardiography to evaluate cardiac function and were then euthanized on the 21st day of the experiment.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of serum cardiac troponin T,creatine kinase-myocardial band,B-type natriuretic peptide,and cytokine levels.Myocardial tissue inflam-mation infiltration and myocardial pathological scores were evaluated by hematoxylin-eosin(HE)staining.The levels of CD4+T cell subsets in the rat spleen were detected by flow cytometry.The expressions of SOCS3,Janus kinase 2(JAK2),signal transducer and acti-vator of transcription 3(STAT3),and CD4+T cell-specific transcription factors were detected by real time fluorescent quantitative poly-merase chain reaction(RT-qPCR)and Western blot.Results In the EAM group,the levels of serum of cardiac troponin T,creatine kinase-myocardial band,and B-type natriuretic peptide,were significantly increased compared with those in the control group(P＜0.05),and the myocardial pathological score was significantly increased(P＜0.05);In the EAM group,the mRNA and protein expres-sions of T-box expressed in T cells(T-bet)and retinoid-related orphan receptor γt(RORγt)in myocardial tissues were significantly increased compared with those in the control group(all P＜0.05),while the expression of forkhead box P3(Foxp3)was significantly de-creased(P＜0.05);In the EAM group,the percentages of helper T cell 1(Th1)and helper T cell 17(Th17)in the spleen were signif-icantly increased compared with those in the control group(both P＜0.05),and the percentage of regulatory T cell(Treg)was signifi-cantly decreased(P＜0.05);In the EAM group,the mRNA and protein expressions of JAK2 and STAT3 in myocardial tissues were signifi-cantly increased compared with those in the control group(P＜0.05).These indexes in the EAM+SOCS3group were significantly restored compared with those in the EAM group(P＜0.05).Conclusion SOCS3 overexpression can ameliorate the imbalance of CD4+T cells and alleviate myocardial damage in EAM rats,and its mechanism is related to the downregulation of the JAK2/STAT3signaling pathway.

Objective:To explore the application effect of Assessmen-Diagnosis-Plan-Implementation-Evaluation(ADPIE) teaching model based on skill competition in the teaching of "basic nursing" skills in higher vocational colleges.Methods:This was a quasi experimental study. Convenience sampling method was adopted to select students from two classes of 2021 vocational nursing major of Pingxiang Health Vocational College from February to July 2022 as the research objects, among which 53 nursing students from Class 9 were selected as the experimental group and 55 nursing students from Class 13 were selected as the control group. The experimental group was taught based on the ADPIE teaching mode of skill competition. The control group was taught according to the conventional teaching mode, and the academic record and humanistic care ability of the nursing students in the two groups were compared.Results:The three dimensions of humanistic care ability in experimental group were cognition (70.82 ± 9.20) points, courage (59.58 ± 6.04) points, patience (57.17 ± 5.26) points, total score (187.57 ± 20.50) points. They were higher than those in the control group,which were (67.40 ± 8.83), (55.98 ± 9.03), (54.37 ± 5.96) and (177.75 ± 20.82) points, and there were statistical differences between the groups (t values were 2.61 to 3.42, all P<0.05). In addition, the three dimensions of academic performance: ordinary score (6.10 ± 1.22)points, course score (57.10 ± 6.27) points, objective structured clinical examination (OSCE) score (27.09 ± 2.13)points, total score (90.29 ± 9.69) points, they were also higher than those of the control group (5.06 ± 1.33), (52.73 ± 5.44), (25.35 ± 3.29), and (83.14 ± 10.06), and the differences between groups were statistically significant ( t values were 2.52-3.34, All P<0.05). Conclusions:ADPIE teaching model based on skills competition can improve the course scores and humanistic care ability of nursing students, and the teaching effect is significant.

Objective To investigate the status of self-management in patients with chronic heart failure (CHF) in Zhejiang province, and to explore the relationship between the self-management level and medical coping styles. Methods From March to September 2016, using the convenient sampling method, a total of 450 aged patients with chronic heart failure from 5 Class Ⅲ Grade A hospitals in 3 districts including Ningbo, Pinghu and Jiaxing of Zhejiang province, who were hospitalized in the Cardiovascular Department, were recruited as research subjects. The Self-Management Scale of Heart Failure Patients and Medical Coping Modes Questionnaire (MCMQ) were applied in the investigation to carry out the correlation analysis. Results A total of 450 questionnaires were distributed, and 437 valid questionnaires were collected. The Self-Management Scale of Heart Failure Patients in the 437 patients were (51.48±10.77) in total score, (18.90±3.10) in facing coping style, (16.12±2.18) in avoiding coping style and (11.85±1.82) in yielding coping style. Pearson correlation analysis showed that the total score of self-management was positively correlated with facing coping style (r=0.303, P＜0.01), negatively correlated with yielding (r=-0.157, P＜0.01), and positively correlated with avoiding coping style (r=0.117, P＜0.01). Multiple linear stepwise regression analysis showed that family monthly income, self-care ability and marriage were the main factors affecting self-management of patients with chronic heart failure. Conclusions Self-management level in patients with chronic heart failure in Zhejiang province is relatively low, and effected by family income, self-care ability, marriage and other factors. Nursing staff should understand different characteristics of different patients to provide personalized health education, improve their self management level, and promote the patients more actively face the disease, reduce and avoid negative medical coping.

Objective To investigate the role of miR-223 in the pathogenesis of acute gouty inflammation.Methods The subjects were divided into 3 groups:65 acute gout patients (AG),50 inter-critical gout patients (IG),and 45 healthy controls (HC).The peripheral blood mononuclear cells (PBMCs) and the clinical laboratory parameters were all collected.The expression of miR-223 in the PBMCs was detected using realtime fluorescent quantitative polymerase chain reaction (RT-qPCR) (TaqMan probe).The PBMCs of 5 healthy people were stimulated with monosodium urate (MSU) (100 μg/ml) for 12 h,and then,miR-223,NLRP3 mRNA and IL-1β production were all measured using RT-qPCR and ELISA respectively.All data were analyzed by SPSS 17.0 statistical software,Wilcoxon rank sum test,t test and Spearman's correlations analysis were used for statistical analysis.Results ① The expression of miR-223 in AG and IG groups was both significantly decreased than that in the HC group (8±17 vs 26±76,P＜0.05;9±17 vs 26±76,P＜0.05;respectively),AG group was significantly decreased than that in the IG group [8(17) vs 9(17),Z=11.387,P＜0.01].② After stimulated with MSU in healthy controls,IL-1β production and NLRP3 mRNA were both significantly increased [(86±5) pg/ml vs (13±6) pg/ml,t=21.042,P＜0.01;5.2±0.4 vs 1.2±0.4,t=14.640,P＜0.01;respectively],while the expression of miR-223 was significantly decreased (0.34±0.20 vs 1.05±0.24,t=-5.164,P＜0.01).Conclusion The data suggests that miR-223 might be involved in the patho-genesis of spontaneous regulation,but further study is needed to discover the exact mechanism.

OBJECTIVE:To observe clinical efficacy and safety of thymosin in adjunctive treatment of chronic eczema,and to investigate its mechanism from the change of peripheral T lymphocyte subsets. METHODS:160 patients with chronic eczema were randomly divided into observation group and control group,with 80 cases in each group. Both group received conventional therapy as mizolastine,Desonide cream. Observation group additionally received Thymosin enteric-coated tablet 20 mg,po,tid;control group was given placebo the appearance of which was same with Thymosin enteric-coated tablet. Treatment course lasted for 4 weeks. The change of T lymphocyte subsets,clinical efficacy and the occurrence of ADR were compared between 2 groups. Re-currence rate of both groups were followed up for 1 year. RESULTS:The effective rate of observation group(91.25%)was signifi-cantly higher than that of control group(77.50%),with statistical significance(P<0.05). After treatment,CD4+ and CD4+/ CD8+ of 2 groups were significantly increased,while CD8+ was decreased significantly;CD4+ and CD4+/ CD8+ of observation group were significantly higher than those of control group,with statistical significance(P<0.05),but there was no statistical significance in CD8+ between 2 groups(P>0.05). There was no statistical significance in the incidence of ADR between 2 groups(P>0.05). Re-currence rate of observation group(31.51%)was significantly lower than that of control group(64.52%)during follow-up period, with statistical significance (P<0.05). CONCLUSIONS:In summary,thymosin can improve clinical efficacy and reduce recur-rence rate by regulating T lymphocyte subsets with good safety.

Objective To measure the operative length of the radioactive source with RL_HZJ18 afterloading machine.Methods The ways of direct measurement and treatment plan are both collected to measure.Results Both of the ways can measure the operative length,and the error of length can be accurate to 1mm.Conclusion The way is simple,practical,good maneuverability,it is worth the trial and promotion.