Objective:To investigate the effect of olanzapine (OLZ) on the differentially-expressed circRNAs in prefrontal cortex of schizophrenia mouse models and predict the target genes.Methods:SPF grade C57BL/6 mice, 7~8 weeks-old, 20 male mice and 45 female mice were recruited and breeded offspring.Forty-four double-stimulation induced schizophrenia-like mouse models, the offspring mice exposed to dual stress were divided into the schizophrenia group(SZ group, n=23) and the olanzapine intervention group (SZ+ OLZ group, n=21), while the mice raised under normal conditions served as the control group (NC group, n=22). Whole transcriptome sequencing was used to sequence the expression level of RNAs from the prefrontal cortex of the mice. RT-qPCR was applied to verify the differentially-expressed circRNAs, then the target genes of miRNAs which have binding site to verified circRNAs were predicted. Results:RNA-seq results showed that there were 137 differentially-expressed circRNAs compared with NC group, 62 were significantly high-expressed and 75 were low-expressed. circRNA_06886 showed significant low-expressed in SZ group compared with NC group( Z=-3.259, P<0.01), and significant high-expressed in SZ+ OLZ group compared with SZ group( Z=-4.765, P<0.01). Bioinformatics analysis of miRNA target genes showed that the target genes were involved in the pathways related to neural pathways such as dopamine, glutamate and MAPK signaling pathways. Conclusions:There are differentially expressed circRNAs in the prefrontal cortex of schizophrenia mouse models, and circRNA_06886 is low-expressed in the prefrontal cortex of schizophrenia mice, Camk2b-201 and Plcb1-003 are the potential genes of circRNA_06886 involved in the regulation of schizophrenia pathogenesis by dopamine pathway.

Objective To explore the current status and correlation between job embeddedness and turnover intention among healthcare workers in public hospitals in Guangzhou,providing empirical evidence for public hospital administrators to re-duce talent turnover.Methods This study surveyed 718 healthcare workers from 12 public tertiary hospitals in Guangzhou.Results The overall mean score for job embeddedness among healthcare workers was(3.78±0.84),and the overall mean score for turnover intention was(1.96±0.94).There was a significant negative correlation between job embeddedness and turn-over intention(r=-0.561,P＜0.01),indicating that a higher level of job embeddedness corresponded to a lower turnover in-tention among healthcare workers.Additionally,factors such as age,marital status,number of children,monthly income,and years of work in the current hospital had significant effects on turnover intention.Conclusion The level of job embeddedness a-mong healthcare workers is moderate,and the turnover intention is relatively low,with a significant negative correlation between the two.In the context of high-quality development of public hospitals,the stability of medical talent is crucial,as excessive tal-ent turnover can increase costs and affect operational and service quality.Suggestions Hospital management should improve com-pensation and benefits,optimize salary structures,and increase benefit programs.They should also provide career development opportunities,establish a career development planning system,optimize the work environment,reasonably arrange work sched-ules,and provide good facilities.Furthermore,strengthening community connections,encouraging participation in community ac-tivities,and focusing on mental health by providing psychological counseling services are recommended.

Objective:To investigate the effect of olanzapine (OLZ) on the differentially-expressed circRNAs in prefrontal cortex of schizophrenia mouse models and predict the target genes.Methods:SPF grade C57BL/6 mice, 7~8 weeks-old, 20 male mice and 45 female mice were recruited and breeded offspring.Forty-four double-stimulation induced schizophrenia-like mouse models, the offspring mice exposed to dual stress were divided into the schizophrenia group(SZ group, n=23) and the olanzapine intervention group (SZ+ OLZ group, n=21), while the mice raised under normal conditions served as the control group (NC group, n=22). Whole transcriptome sequencing was used to sequence the expression level of RNAs from the prefrontal cortex of the mice. RT-qPCR was applied to verify the differentially-expressed circRNAs, then the target genes of miRNAs which have binding site to verified circRNAs were predicted. Results:RNA-seq results showed that there were 137 differentially-expressed circRNAs compared with NC group, 62 were significantly high-expressed and 75 were low-expressed. circRNA_06886 showed significant low-expressed in SZ group compared with NC group( Z=-3.259, P<0.01), and significant high-expressed in SZ+ OLZ group compared with SZ group( Z=-4.765, P<0.01). Bioinformatics analysis of miRNA target genes showed that the target genes were involved in the pathways related to neural pathways such as dopamine, glutamate and MAPK signaling pathways. Conclusions:There are differentially expressed circRNAs in the prefrontal cortex of schizophrenia mouse models, and circRNA_06886 is low-expressed in the prefrontal cortex of schizophrenia mice, Camk2b-201 and Plcb1-003 are the potential genes of circRNA_06886 involved in the regulation of schizophrenia pathogenesis by dopamine pathway.

Objective To explore the current status and correlation between job embeddedness and turnover intention among healthcare workers in public hospitals in Guangzhou,providing empirical evidence for public hospital administrators to re-duce talent turnover.Methods This study surveyed 718 healthcare workers from 12 public tertiary hospitals in Guangzhou.Results The overall mean score for job embeddedness among healthcare workers was(3.78±0.84),and the overall mean score for turnover intention was(1.96±0.94).There was a significant negative correlation between job embeddedness and turn-over intention(r=-0.561,P＜0.01),indicating that a higher level of job embeddedness corresponded to a lower turnover in-tention among healthcare workers.Additionally,factors such as age,marital status,number of children,monthly income,and years of work in the current hospital had significant effects on turnover intention.Conclusion The level of job embeddedness a-mong healthcare workers is moderate,and the turnover intention is relatively low,with a significant negative correlation between the two.In the context of high-quality development of public hospitals,the stability of medical talent is crucial,as excessive tal-ent turnover can increase costs and affect operational and service quality.Suggestions Hospital management should improve com-pensation and benefits,optimize salary structures,and increase benefit programs.They should also provide career development opportunities,establish a career development planning system,optimize the work environment,reasonably arrange work sched-ules,and provide good facilities.Furthermore,strengthening community connections,encouraging participation in community ac-tivities,and focusing on mental health by providing psychological counseling services are recommended.

Objective:To establish neurodevelopmental mice model of schizophrenia(SZ) with prepulse inhibition(PPI) deficits and investigate the effectiveness of olanzapine on PPI disruption.Methods:On the 9th day of pregnancy of SPF grade C57BL/6 mice, female mice were injected with polyinosinic acid poly (I∶C) (6 mg/kg) through tail vein for immune stimulation. The stress model was constructed by chronic unpredictable mild stress 30-40 d after birth (PND30-40). The offspring mice were divided into pregnancy immune stimulation + adolescent stress group (P + S + group), pregnancy immune stimulation group (P + S- group), adolescent stress group (P-S+ group) and non stimulation group (P-S-group), with 18 mice in each group. The mice in P+ S+ group were divided into OLZ intervention group (OLZ group) and non-OLZ intervention group (non-OLZ group), with 9 mice in each group. The PPI function of mice was detected by acoustic startle reflex test after modeling and OLZ intervention. Adopt StatView Version 5.0 software for data analysis, and multi factors analysis of variance was used to test the main effect, interactive effect and simple effect of each factor.Results:The main effects of maternal Poly(I: C) immune activation and pubertal chronic unpredictable stress were significant( F(1, 330)=47.72, P<0.01), and there was a significant interaction between the two factors( F(1, 330)=14.80, P<0.01), simple effect analysis showed that average percent prepulse inhibition (PPI%) in P+ S+ group((15.42±6.13)%) was significantly decreased compared with groups of P+ S-((27.33±4.58)%), P-S+ ((31.17±3.97)%) and P-S-((47.14±12.28)%)(all P<0.01). There was significant gender difference in Prepulse inhibition(PPI)score( F(1, 396)=61.94, P<0.01), in male and female mice, average startle reactivity of Pulse under Prepulse+ Pulse influence of distinct intensities was significantly different( F(1, 198)=18.68, 18.44, P<0.01), and the maternal Poly(I∶C) immune activation had a significant main effect( F(1, 198)=32.18, 12.58, P<0.01) and interaction with pubertal chronic unpredictable stress( F(1, 198)=34.54, 11.39, P<0.01), simple effect analysis suggested that the average startle reactivity of Prepulse+ Pulse in P+ S+ group(0.47±0.12) was significantly higher than other three groups(P+ S-: 0.36±0.11, P-S+ : (0.25±0.22), P-S-: (0.31±0.19)) in male mice( P<0.01) and in P-S+ group was significantly higher than the other three groups in female mice ( P<0.01). OLZ treatment could efficiently reverse the deficits on PPI by increasing PPI%( F(1, 165)=18.24, P<0.01), OLZ could reduce PPI score in male "dual-hit" model mice( F(1, 102)=21.81, P<0.01)and raise it in female( F(1, 102)=4.88, P<0.05). Conclusion:OLZ can reverse PPI deficits in schizophrenic neurodevelopmental model mice, and in the evaluation of PPI function in the model mice through PPI of acoustic startle reflex, PPI% has better stability and reactivity to OLZ intervention.

Objective: To evaluate the effectiveness of deep learning model trained on routine CT scans when identity the malignant and benign lung nodule on target CT scans dataset. Methods: This retrospective study enrolled 923 patients with lung nodules found by chest CT scan in Shanghai Chest Hospital from January 2016 to December 2018. A total of 969 nodules with pathological report were analyzed. The deep learning based pulmonary malignant prediction method in a fine-grained classification manner was used to make the prediction, and the AUC (the area under the curve), accuracy, sensitivity and specificity of routine CT scans and target CT scans were compared, and Delong test and IDI (Integrated Discrimination Improvement) were employed to provide statistical results. Furthermore, statistical methods were used to investigate the differences between the benign and malignant classification of nodules on routine CT and on target CT. Results: In the benign and malignant discrimination task, AUC, accuracy, sensitivity and specificity on the routine scans were 0.81, 82.0%, 86.0% and 56.6% respectively, while the AUC, accuracy, sensitivity and specificity on the target scans were 0.84, 85.0%, 88.8% and 60.5% respectively. The IDI was 0.056 (Z test, P<0.05), and there was statistically significant difference in ROC (Delong test, P=0.01). Conclusions The deep learning model trained on the data set of routine CT scans can achieve better diagnostic efficiency in target CT scans data.

Objective: To explore the relationship of trait anxiety, gender difference and risk averse. Methods: A test was conducted fwstly in 328 college students with the State-Trait Anxiety Inventory. The participants with a score≥53 were termed as high trait-anxiety group (N=30), in contrast to the former, the par-ticipants with a score ≤ 30 were treated as the low trait-anxiety (N=30) . The material was 10 hypothesized risk settings, asking the participants to do decision-making, at the same time, the participants were also required to ac-cess their subjective cost if the result of their decision was negative; recording the scores and the reaction time of the participants. Results: The risk-avoidant score in high trait-anxiety group was significantly higher than that in low trait-anxiety group [(5.97±1.53) vs.(3.27±1.25), P <0.01], and the risk-avoidant score in males was sig-nificantly higher than that in females [(2.78±1.37) vs.(6.46±1.26), P <0.01] .There also had significant interaction effects between the 2 variables (F=7.43, P <0.01). The reaction time in males was also longer than that in females [high-trait anxiety: (1586.47±10.352)vs.(1067.52±18.468); low-trait anxiety: (643.46±36.759)vs.(498.36±9.673); P < 0.01] . The correlation between subjective loss and trait-anxiety in females was higher than that in males (Z=2.15, P<0.05) . Conclusion: There is significant effect of trait-anxiety and gender difference on risk-avoidant decision-making. The high-trait and female individuals have more risk-avoidant in decision-making.