Objective To investigate the predictive value of serum circular RNA(circRNA)CDYL and circRNA ACAP2 expression levels for major adverse cardiovascular events(MACE)in pa-tients with acute myocardial infarction(AMI).Methods A total of 98 AMI patients were enrolled into the observation group,and divided into MACE group(n=45)and no-MACE group(n=53)based on whether they experienced MACE.Another 98 healthy individuals who underwent physical ex-amination during the same period were selected as the control group.The expression levels of serum cir-cRNA CDYL and circRNA ACAP2 were compared among groups.Multivariate logistic regression analysis was used to identify factors influencing the occurrence of MACE in AMI patients.Receiver operating characteristic(ROC)curve analysis was conducted to evaluate the predictive value of serum circRNA CDYL and circRNA ACAP2 levels for MACE in AMI patients.Results The serum circRNA CDYL level in the observation group was significantly lower than that in the control group,while the circRNA ACAP2 level was significantly higher(P＜0.05).The circRNA CDYL level in the MACE group was significantly lower,and the circRNA ACAP2 level was significantly higher than that in the no-MACE group(P＜0.05).Heart rate and circRNA ACAP2 were identified as independent risk factors for MACE in AMI patients,whereas circRNA CDYL served as independent protective factor(P＜0.05).The area under the ROC curve(AUC)for predicting MACE in AMI patients using se-rum circRNA CDYL,circRNA ACAP2 and their combination were 0.814,0.821 and 0.921,re-spectively.The sensitivity and specificity of combined prediction using serum circRNA CDYL and circRNA ACAP2 were 91.11％and 79.25％,respectively.The combined prediction efficacy of serum circRNA CDYL and circRNA ACAP2 was superior to their individual prediction(Zcombined-circRNA CDYL=1.975,Zcombined-circRNAACAP2=2.064,P=0.048,0.039).Conclusion Serum circRNA CDYL lev-el is down-regulated and circRNA ACAP2 level is up-regulated in AMI patients,and circRNA ACAP2 is an independent risk factor for MACE,while circRNA CDYL is a independent protective factor.The combined value of circRNA CDYL and circRNA ACAP2 in predicting the occurrence of MACE is higher.

OBJECTIVE To investigate the effect of Curcumol on lipid metabolism in non-small cell lung cancer(NSCLC)and its related molecular mechanism.METHODS A nude mouse xenograft tumor model was established,and Curcumol was administered for 14 d.The volume and weight of subcutaneous tumors were recorded.Hematoxylin-eosin(HE)and immunohistochemical staining were used to observe the pathological morphology of tumor tissues and organs,as well as the expression levels of Ki67 protein.Bio-chemical kits were used to detect the levels of lipids in tumor tissues.Western blot was used to detect the expression levels of SREBP-1,FASN,and EGFR proteins in tumor tissues.The qPCR and immunofluorescence were used to detect the expression of SREBP-1 in tumor tissues.Curcumol was used to treat human NSCLC cell line A549 and A549 cells overexpressing SREBP-1.The EdU assay was used to detect the proliferation ability of lung cancer cells;the MTT colorimetric method was used to detect cell viability;the Transwell assay was used to detect tumor cell migration and invasion;flow cytometry was used to detect cell apoptosis;biochemical kits were used to detect lipid levels in cells;and Western blot was used to detect the expression levels of SREBP-1,FASN,and EGFR proteins.RE-SULTS In the in vivo experiment,compared with the control group,Curcumol significantly inhibited the growth of subcutaneous xen-ografts,inhibited tumor cell proliferation(P<0.05,P<0.01),reduced the levels of triglycerides(TG),total cholesterol(T-CHO),and free fatty acids(FFA)in tumor tissues(P<0.05,P<0.01),downregulated the expression of SREBP-1,FASN,and p-EGFR pro-teins(P<0.01),and inhibited the expression of SREBP-1 mRNA in tumor tissues(P<0.05,P<0.01).In the in vitro experiment,compared with the control group,Curcumol significantly inhibited the proliferation,migration,and invasion of A549 cells,reduced cell viability(P<0.05,P<0.01),promoted tumor cell apoptosis(P<0.01),downregulated the levels of FFA,T-CHO,and TG in A549 cells(P<0.05,P<0.01),and inhibited the expression of SREBP-1,FASN,and p-EGFR proteins(P<0.05,P<0.01).Additionally,overexpression of SREBP-1 antagonized the inhibitory effects of Curcumol on lipid synthesis and EGFR activation,and weakened the inhibitory effects of Curcumolc on tumor cells.CONCLUSION Curcumol inhibits the expression levels of SREBP-1 and FASN,re-duces lipid synthesis,blocks EGFR signal transduction,and slows down the occurrence and development of NSCLC.

OBJECTIVE To investigate the effect of Curcumol on lipid metabolism in non-small cell lung cancer(NSCLC)and its related molecular mechanism.METHODS A nude mouse xenograft tumor model was established,and Curcumol was administered for 14 d.The volume and weight of subcutaneous tumors were recorded.Hematoxylin-eosin(HE)and immunohistochemical staining were used to observe the pathological morphology of tumor tissues and organs,as well as the expression levels of Ki67 protein.Bio-chemical kits were used to detect the levels of lipids in tumor tissues.Western blot was used to detect the expression levels of SREBP-1,FASN,and EGFR proteins in tumor tissues.The qPCR and immunofluorescence were used to detect the expression of SREBP-1 in tumor tissues.Curcumol was used to treat human NSCLC cell line A549 and A549 cells overexpressing SREBP-1.The EdU assay was used to detect the proliferation ability of lung cancer cells;the MTT colorimetric method was used to detect cell viability;the Transwell assay was used to detect tumor cell migration and invasion;flow cytometry was used to detect cell apoptosis;biochemical kits were used to detect lipid levels in cells;and Western blot was used to detect the expression levels of SREBP-1,FASN,and EGFR proteins.RE-SULTS In the in vivo experiment,compared with the control group,Curcumol significantly inhibited the growth of subcutaneous xen-ografts,inhibited tumor cell proliferation(P<0.05,P<0.01),reduced the levels of triglycerides(TG),total cholesterol(T-CHO),and free fatty acids(FFA)in tumor tissues(P<0.05,P<0.01),downregulated the expression of SREBP-1,FASN,and p-EGFR pro-teins(P<0.01),and inhibited the expression of SREBP-1 mRNA in tumor tissues(P<0.05,P<0.01).In the in vitro experiment,compared with the control group,Curcumol significantly inhibited the proliferation,migration,and invasion of A549 cells,reduced cell viability(P<0.05,P<0.01),promoted tumor cell apoptosis(P<0.01),downregulated the levels of FFA,T-CHO,and TG in A549 cells(P<0.05,P<0.01),and inhibited the expression of SREBP-1,FASN,and p-EGFR proteins(P<0.05,P<0.01).Additionally,overexpression of SREBP-1 antagonized the inhibitory effects of Curcumol on lipid synthesis and EGFR activation,and weakened the inhibitory effects of Curcumolc on tumor cells.CONCLUSION Curcumol inhibits the expression levels of SREBP-1 and FASN,re-duces lipid synthesis,blocks EGFR signal transduction,and slows down the occurrence and development of NSCLC.

OBJECTIVETo analyze the long-term effect of primary combined tissue transplantation on hand reconstruction.

METHODSThe data of 8 kinds of combined tissue transplantations employed to reconstruct the severely injured hands of 26 patients over the past 2 to 11 years were studied retrospectively. Among them, combined tissue transplantation taking the anterior-lateral femoral flap as the main tissue unit was applied in 21 cases and taking the second toe as the main tissue unit was applied in 5 cases. Blood vessel anastomosis was performed in parallel in 16 cases, series in 6 cases and both in 4 cases.

RESULTSAmong the 60 free tissue units employed on 26 patients, 58 survived completely and the other 2 survived after dressing change because of postoperative partial necrosis. The patients were followed up for 2-11 years postoperatively, with an average of 3.5 years. According to the standard for function of reconstructed hands by Chinese Medical Association, excellent results were obtained in 10 cases, good in 12 cases, fair in 3 cases and bad in 1 case.

CONCLUSIONSPrimary combined tissue transplantation, which may preserve the tissue vitality of injured hands to the maximum and thus facilitate function restoration of the hands, is a promising method in reconstructing severely-injured hands.

Adolescent ; Adult ; Child ; Female ; Hand Injuries ; surgery ; Humans ; Male ; Middle Aged ; Reconstructive Surgical Procedures ; Surgical Flaps ; Treatment Outcome