Objective To observe the dynamic changes of molecules related to the Nrf2/Keap1/p62 signaling pathway in patients with postoperative pulmonary inflammation after thoracic surgery,and explore its relationship with prognosis.Methods Sixty patients with postoperative pneumonia after thoracic surgery who met the criteria in the Department of Thoracic Surgery of Taihe County Hospital of Traditional Chinese Medicine from June 2023 to June 2024 were selected as the research subjects.Venous blood was collected from the pa-tients on the day before surgery,the first day after surgery,the third day after surgery,and the seventh day after surgery to detect the lev-els of superoxide dismutase(SOD),nitric oxide(NO),malondialdehyde(MDA),reactive oxygen species(ROS)in peripheral blood,as well as the expression of Nrf2,Keap1,and p62 mRNA.The correlations between these indicators and the prognosis of postoperative pulmonary infection after thoracic surgery were analyzed.Results The SOD level of the patients decreased after the operation compared with that before the operation,reaching the lowest value on the first day after the operation.The levels of MDA and ROS increased after the operation,but slowly decreased on the seventh day after the operation.There was no significant change in the NO level compared with that before the operation.Compared with before the operation,the expression levels of Nrf2 and Keap1 in the patients decreased signifi-cantly on the first day after the operation,and then slowly increased.The expression level of p62 increased to the peak on the first day af-ter the operation,and then decreased.The results of lung function index measurement showed that the patients had pulmonary infection af-ter the operation,and the lung function level decreased significantly compared with that before the operation.Through ROC curve analy-sis,the dynamic changes of SOD,ROS,Nrf2,Keap1 and p62 in the patients with pulmonary inflammation after thoracic surgery have good predictive value for prognosis.Conclusion The changes of Nrf2/Keap1/p62 signaling pathway-related factors in patients with post-thoracic surgery-induced pulmonary inflammation are significantly correlated with the prognosis of these patients.Such changes can effectively predict the prognosis of patients from the perspective of the expression levels of related genes.

Objective To observe the dynamic changes of molecules related to the Nrf2/Keap1/p62 signaling pathway in patients with postoperative pulmonary inflammation after thoracic surgery,and explore its relationship with prognosis.Methods Sixty patients with postoperative pneumonia after thoracic surgery who met the criteria in the Department of Thoracic Surgery of Taihe County Hospital of Traditional Chinese Medicine from June 2023 to June 2024 were selected as the research subjects.Venous blood was collected from the pa-tients on the day before surgery,the first day after surgery,the third day after surgery,and the seventh day after surgery to detect the lev-els of superoxide dismutase(SOD),nitric oxide(NO),malondialdehyde(MDA),reactive oxygen species(ROS)in peripheral blood,as well as the expression of Nrf2,Keap1,and p62 mRNA.The correlations between these indicators and the prognosis of postoperative pulmonary infection after thoracic surgery were analyzed.Results The SOD level of the patients decreased after the operation compared with that before the operation,reaching the lowest value on the first day after the operation.The levels of MDA and ROS increased after the operation,but slowly decreased on the seventh day after the operation.There was no significant change in the NO level compared with that before the operation.Compared with before the operation,the expression levels of Nrf2 and Keap1 in the patients decreased signifi-cantly on the first day after the operation,and then slowly increased.The expression level of p62 increased to the peak on the first day af-ter the operation,and then decreased.The results of lung function index measurement showed that the patients had pulmonary infection af-ter the operation,and the lung function level decreased significantly compared with that before the operation.Through ROC curve analy-sis,the dynamic changes of SOD,ROS,Nrf2,Keap1 and p62 in the patients with pulmonary inflammation after thoracic surgery have good predictive value for prognosis.Conclusion The changes of Nrf2/Keap1/p62 signaling pathway-related factors in patients with post-thoracic surgery-induced pulmonary inflammation are significantly correlated with the prognosis of these patients.Such changes can effectively predict the prognosis of patients from the perspective of the expression levels of related genes.

Objective To investigate the effect of narrow-band imaging combined with endoscopic submucosal dissection in the treatment of lateral developmental tumors(LST)of the large intestine and the analysis of postoperative recurrence.Method 78 LST patients admitted to Changzhou Traditional Chinese Medicine Hospital between April 2019 and April 2022 were retrospectively selected,and the surgical methods were selected according to the patients'wishes.According to the surgical methods,they were divided into observation group(narrowband imaging combined with endoscopic submucosal dissection,n=39)and control group(endoscopic submucosal dissection,n=39).The perioperative period and postoperative recovery,tumor resection,postoperative complications and quality of life were compared.Result The operation time of the observation group was(68.16±10.13)minutes,the recovery time of defecation was(51.34±6.36)hours,the exhaust time was(47.28±10.78)hours,and the recovery time of bowel sound was(32.67±6.02)hours.In the control group,they were(76.03±10.11)minutes,(54.55±6.27)hours,(52.56±11.55)hours and(35.84±5.97)hours,respectively,and there was statistical significance between the two groups(P＜0.05).The overall resection rate of observation group(39 cases/39 cases)was higher than that of control group(32 cases/39 cases),and the difference was statistically significant(P＜0.05).The total rate of intestinal infection,dyspepsia and adverse reactions in observation group(5 cases/39 cases)was lower than that in control group(18 cases/39 cases),with statistical significance(P＜0.05).The mental health scores of the observation group were(76.16±12.13),emotional function scores were(77.32±11.13),social function scores were(80.37±11.13),vitality score was(81.23±9.07),body pain score was(83.15±8.15),the physiological function score was(82.09±10.28)and the physiological function score was(85.27±8.28).the scores of control group were(62.34±11.09),(65.50±12.13),(72.18±10.07),(75.10±9.11),(76.11±7.07),(76.11±7.07)and(75.14±8.32),respectively.There was statistical significance between the two groups(P＜0.05).Conclusion The combination of narrowband imaging technology and endoscopic submucosal dissection has a good therapeutic effect on laterally spreading tumor,with fewer postoperative complications.

Objective:To explore significance of eosinophil and eosinophil/lymphocyte ratio(ELR)in predicting allergic asth-ma in children in different seasons.Methods:Retrospective analysis of children with asthma who visited pediatric respiratory depart-ment outpatient clinic and ward of Shandong Provincial Hospital Affiliated to Shandong First Medical University from January 2021 to December 2021,whose allergen specific IgE(sIgE)and peripheral blood cell analysis were complete.sIgE≥0.35 kUA/L were included in allergic asthma group,sIgE<0.35 kUA/L and total allergen IgE<60 kUA/L were included in non-allergic asthma group.General data and changes in peripheral blood cells of two groups were analyzed.Results:There were 1 378 qualified subjeats,including 999(72.5%)in allergic asthma group and 379(27.5%)in non-allergic asthma group.Number of visits in allergic asthma group varied seasonally,with the most in autumn.Peripheral blood lymphocyte count(LYMPH),eosinophil count(EOS)and ELR were all higher in children with allergic asthma than in children with non-allergic asthma(P<0.05),and platelet/lymphocyte ratio(PLR)was lower than that in children with non-allergic asthma(P<0.05).Peripheral blood LYMPH,PLT,EOS and ELR of children with allergic asthma differed between four seasons,which were higher than those of non-allergic asthma in each season in EOS and ELR,LYMPH was significantly higher than that of children with non-allergic asthma in autumn,and PLT was significantly lower than that of children with non-allergic asthma in spring(P<0.05).EOS predicted AUC of spring,summer,autumn and winter were 0.79,0.77,0.71 and 0.64 in children with allergic asthma,and ELR predicted AUC were 0.72,0.48,0.73 and 0.68 in children with allergic asthma.Conclusion:Allergic asthma in children is seasonally variable and peaks in autumn.EOS and ELR in peripheral blood cells in children with allergic asthma are higher than in children with non-allergic asthma in each season of year,LYMPH is significantly higher than children with non-allergic asthma in the fall,and PLT is lower than in children with non-allergic asthma in spring,suggesting that allergic asthma type Ⅱinflammation persists,and EOS and ELR have predictive value for children's allergic asthma.

Objective:To establish the microbial limit test methods for thirteen kinds of ointments. Methods:The microbial limit of 13 kinds of ointments was respectively determined by the routine method, culture medium dilution method and membrane filtration method. Results:The recovery of the tested bacteria in the samples was above 70% by the different methods. Conclusion:The micro-bial limit test methods for thirteen kinds of ointments are stablished, which may be used in the quality control.

Objective To investigate current status of learned resourcefulness in pregnant women,and to identify its influencing factors.Methods With convenient sampling,a self-made General Data Questionnaire and the Chinese version of Self-Control Schedule (C-SCS) was used to survey 360 pregnant women in the antenatal clinic in one three-class hospital in Guangzhou.Results The mean score of learned resourcefulness in pregnant women was (115.00±14.42) and its influencing factors included education degree,gravidity,pregnancy school training and planed pregnancy.Conclusions Learned resourcefulness in pregnant women loads upper level.Medical staff should take targeted interventions to strengthen it and facilitate the mental well-being of pregnant women.

OBJECTIVETo reconstruct mandibular defect using tissue engineering bone with bone marrow stem cells (BMSCs) cell sheets and investigate the effect of cell sheets on osteogenesis.

METHODSBMSCs were isolated with the method of density gradient centrifugation from canine and cultured. BMSCs were induced to differentiate to osteoblasts. BMSCs induced were fabricated to BMSCs cell sheets. The poly (lactic-co-glycolic acid) (PLGA) wrapped with cell sheets were implanted into the mandibular defect in the left side (experimental side). PLGA wrapped without cell sheets were implanted into the right side (control side) of mandibles. 16 dogs were evenly divided into 4 groups, and one group of them was executed in 4, 8, 12, 16 weeks for gross investigation and histological observation.

RESULTSThe osteogenesis of experimental side was better than that of control side. 16 weeks after implantation, most areas of the mandibular defect were replaced by fresh bone tissue. Compact bone similar to normal bone tissue formed in the lingual defect of mandible and had bony union with the bone stump. The optical density of the fresh bone in the experimental side was higher than that of the control side, there was a significant difference between the two methods (P<0.05). Plenty of lamellar bones formed in experimental side and Haversian system, as well as red marrow, were observed.

CONCLUSIONTissue engineering bone with the structure of lamellar bones can be formed by the technology of BMSCs cell sheets.

Animals ; Bone Marrow Cells ; Bone and Bones ; Dogs ; Lactic Acid ; Mandible ; Osteoblasts ; Osteogenesis ; Polyesters ; Polyglycolic Acid ; Polymers ; Tissue Engineering