Objective To investigate the expression and significance of carbohydrate response element-binding protein(ChREBP)network members(ChREBP,Mlx,Max,Mxd1,HDAC1)and taurine-upregulated gene 1(TUG1)in intrauterine adhesion(IUA).Methods Ten female SD rats with 8 weeks old were selected as the study objects.The mechanical curettage plus lipopolysaccharide infection were used to construct the IUA rat model as the IUA group(n=5)and the uterine cavity of the control group did not perform any oper-ation(n=5);HE and Masson staining were respectively used to assess the number of endometrial glands and the degree of fibrosis;Western blot was used to detect the expression level of ChREBP network members in the nucleus and cytoplasm;RT-qRCR was used to detect the expression level of TUG1.Results Compared with the control group,the number of endometrial glands in the IUA group was decreased(P＜0.05),the percentage of the fibrotic area was increased(P＜0.05),the protein expression level of ChREBP network members in the nucleus was down-regulated,the protein expression level of ChREBP network members in the cytoplasm was up-regulated and the mRNA relative expression level of TUG1 was up-regulated(P＜0.05).Conclusion Blocked nuclear translocation of ChREBP and reduced the recruitment of its downstream network mem-bers may lead to attenuate the repressive effect on TUG1 transcription,thereby promoting IUA occurrence.

Objective To investigate the treatment of neonatal respiratory distress syndrome by less invasive surfactant administration(LISA)clinical efficacy,complications and influence on blood gas analysis.Methods A total of 100 cases of neonatal respiratory distress syndrome requiring pulmonary surfactant(PS)tracheal injection therapy and admitted to the Jinhua Central Hospital between December 1st 2019 and August 1st 2022 were randomly assigned into treatment group(50 cases)and control group(50 cases).The treatment group of children received PS treatment using LISA technology,while the control group of children received PS treatment using tracheal intubation tracheal instillation PS extubation followed by intubation-surfactant-extubation technology.The clinical treatment effect,blood gas analysis,non-invasive ventilation time,length of hospitalization,and incidence of complications were compared between the two groups.Results The total effective rate of treatment in the treatment group was higher than that in the control group;After 1 hour of PS treatment,the oxygen partial pressure and blood oxygen saturation of the treatment group were higher than those of the control group,while the carbon dioxide partial pressure was lower than that of the control group.The non-invasive assisted ventilation time of the treatment group was shorter than that of the control group.The incidence of bronchopulmonary dysplasia was lower than that of the control group.Their difference were statistically significant(P<0.05).Conclusion The use of LISA technology for PS treatment of NRDS can improve blood gas indicators,shorten non-invasive ventilation time,improve diagnostic and therapeutic effects,reduce the risk of bronchopulmonary dysplasia,and do not increase the incidence of complications such as tracheal mucosal injury and pulmonary hemorrhage in children.

Objective To construct a vimentin glycosylated mutant plasmid,and to study its impact on rat adrenal chromaffin cell (PC12) cell differentiation.Methods The vimentin glycosylated mutant plasmid was constructed,and the gel electrophoretic was performed and the sequencing identification was performed. The empty plasmid pcDNA3.1b (control group),vimentin plasmid (Vim group) and vimentin glycosylated mutant plasmid (Vim mut group) were used to transfect PC12 cells,which were cultured for 0,3 d in the con-dictions of treatment and non-treatment of exogenous oligosaccharides (Cyclo-ManN pro) respectively,and the cell differentiation records and quantitative analysis (the cell average neurite length and differentiated neu-rite cells percentage) were performed.Results A recombinant plasmid containing a glycosylation site muta-tion site 19(T-G),97(A-G),100(T-G) was successfully constructed (about 1404 bp in size).Before and after Cyclo-ManN pro treatment,the average neurite length of PC12 cells and the percentage of differentiated neu-rite cells in the Vim group were greater than those in the control group and the Vim mut group[(61.98±19.03)μm vs.(51.09±14.45)μm,(51.49±14.78)μm,(6.60±0.25)％ vs. (4.27±0.18)％,(4.76±0.33)％;(78.01±18.31)μm vs. (69.98±12.85)μm,(68.45±13.84)μm,(10.62±0.25)％ vs. (8.11±1.22)％,(5.89±0.60)％],the difference was statistically significant (P<0.05).Conclusion The glycosyla-tion modification of vimentin could be used for PC12 cell differentiation.

Objective To investigate the related effects of three-step prone position on feeding status,gastrointestinal function and growth and development of low birth weight preterm infants.Methods A total of 82 low-birth-weight preterm infants admitted and treated in this hospital from January 2021 to January 2023 were selected as the study subjects.The patients were divided into the observation group(three-step prone position,n=40)and control group(conventional supine position,n=42)according to the feeding body position during hospitalization.The feeding status,length of hospital stay,gastrointestinal complications and growth and development were compared between the two groups.Results Compared with the control group,the time of first time oral feeding beginning in the observation group[(11.63±7.18)d vs.(14.81±6.57)d],the time of oral feeding and then nasal feeding to complete oral feeding[(6.65±2.31)d vs.(8.05±3.45)d],total oral and enteral feeding time[(21.68±9.50)d vs.(25.81±9.21)d]and hospitalization time[(28.68±10.86)d vs.(33.64±10.67)d]all were shorter;the incidence rates of gastroesophageal reflux(7.50％vs.26.19％),gastric retention(10.00％vs.28.57％),vomiting(5.00％vs.21.43％),abdominal distension(5.00％vs.23.81％)]in observation group were lower than those in the control group;the time of recovery to the birth weight was shorter[(7.08±1.33)d vs.(7.83±1.58)d],the daily growth rate of body weight and weekly growth rate of height were greater[(16.45±2.03)g·kg-1·d-1 vs.(15.45±2.35)g·kg-1·d 1,(0.97±0.14)cm/w vs.(0.91±0.11)cm/w],and the differences were statistically significant(P＜0.05).Conclusion The three-step prone position could improve the feeding intolerance,shorten the hospitali-zation stay and promote the growth and development of premature infants.

To explore the process management strategies of standardized residency training based on the development of information management platform, combining with the management practices, this study has sorted out and analyzed process management contents, and integrated process management concepts and contents into the development of information management platform. The development of information management platform taking the process management as the core has contributed to the update and implementation of standardized residency training policies, building of a multi-role interactive bridge, guarantee of information accuracy, and promotion of the construction of quality assurance system, thus providing an effective tool to ensure the quality of training, which is of great significance to cultivate outstanding physicians.