Objective To investigate the relationships between the expressions of miR-134 and vascular endothelial growth factor (VEGF) in gastric cancer with the prognosis of adjuvant chemotherapy gastric cancer after operation.Methods From May 2012 to July 2015,the tumor tissues and non-tumor tissues adjacent to cancer samples of 65 patients with gastric cancer who underwent radical surgery in the oncology department of our hospital were enrolled in this study.Quantitative polymerase chain reaction (qPCR) was used to detect the relative expression of miR-134 and VEGF in tissues,the expression of VEGF was detected by immunohistochemical staining,analyzing the relationships between the expression of miR-134 and VEGF with the clinicopathological characteristics of gastric cancer and the recurrence and metastasis of gastric cancer after adjuvant chemotherapy.Results The relative expression of miR-134 in gastric cancer tissues was lower than that in non-cancer tissues adjacent to cancer,and the relative expression and positive expression rate of VEGF was higher than that in non-cancer tissues adjacent to cancer;there was a negative correlation between the relative expression of miR-134 and VEGF mRNA (r =-0.765,P =0.003);their expression levels were correlated with the invasion of serosa,tumor node metastasis (TNM)stage,and lymph node metastasis of gastric cancer;during the follow-up period the recurrence and metastasis rate of 65 patients with gastric cancer was 41.54％ (27/65);the relative expression of miR-134 in tumor tissues of patients in recurrence and metastasis group was lower than that in non-recurrence and metastasis group (P ＜ 0.05),while the VEGF positive expression rate was higher than that in non-recurrence and metastasis group (P ＜ 0.05);the median tumor-free survival (DFS) in miR-134 low expression group was lower than that in miR-134 high expression group (P ＜0.05),and the DFS of patients with positive expression of VEGF was lower than that of patients with negative expression (P ＜ 0.05);infiltration of serosa,lymph node metastasis,low expression of miR-134,and positive expression of VEGF were risk factors for recurrence and metastasis of gastric cancer after adjuvant chemotherapy.Conclusions The low expression of miR-134 and high expression of VEGF in gastric cancer are closely related to the recurrence and metastasis of gastric cancer after adjuvant chemotherapy and DFS.

PURPOSETo validate that DMPO model for "one step" algorithm can reduce the total MLC segments number, we compare the dosage distribution of "one-step" algorithm with "two-step" algorithm for generating IMRT MLC segments.

METHODSOn the platform of "Pinnacle 8.0h" version radiation treatment planning system developed by Philips, we respectively select one head and neck tumor case, one thorax tumor case and one abdomen tumor case, by means of designing seven fields IMRT planning, then utilized "one-step" algorithm and "two-step" algorithm to optimize and generate IMRT MLC segments, and then calculated dosage distribution, evaluated dosage distribution lines and DVH diagram, in order to compare the two MLC segment groups generated by the two different algorithm.

RESULTFor the three IMRT plans selected by us, the number of MLC segments generated by "two-step" algorithm appear to be larger than "one-step" algorithm but dosage distribution seems to be worse than the latter.

DISCUSSIONFor utilizing the "Pinnacle" plan system to design IMRT plan, "one-step" algorithm with "DMPO" definitely can effectively reduce the number of MLC segments, what's more, the result of dosage distribution seems to be better.

Algorithms ; Radiotherapy Dosage ; Radiotherapy, Intensity-Modulated ; methods

Objective To explore safety of severe preeclampsia by amniotic infusion therapy.Methods 58 cases with severe preeclampsia during 28 to 34 weeks pregnancy had been practised intravenous infusion as controls(37 cases).Results Ultrasound guided amnioinfusion were all succesful in therapy group,there was no maternal complication.Statistical difference was found in premature infant apgar score between therapy group and control group.The incidence of respiratory distress syndrome was 3.4%,whereas that of the control group was 35.1%(P