Background: and Purpose Live cell-based assays (LCBA) are increasingly used for serological antibody detection due to their ability to preserve antigen conformation, offering moderately higher sensitivity than fixed cell-based assays. However, the clinical necessity of prioritizing LCBA for the detection of aquaporin-4 immunoglobulin G (AQP4-IgG) in neuromyelitis optica spectrum disorder (NMOSD) remains unclear, especially when compared to its established role in diagnosing myelin oligodendrocyte glycoprotein antibody-associated disease. Methods: We compared the performance of live cell-based assays using immunofluorescence (LCBA-IF) and fixed cell-based assays using immunofluorescence (FCBA-IF) in detecting AQP4-IgG in 90 cases of NMOSD meeting 2015 International Panel for Neuromyelitis Optica Diagnosis criteria, alongside 40 controls. Additionally, we further investigated the relationship between AQP4-IgG titers as measured by LCBA-IF and FCBA-IF and clinical parameters in NMOSD patients. Results: Results showed 96.9% agreement between LCBA-IF and FCBA-IF (Cohen’s κ=0.935), with a strong Spearman correlation (0.977, p<0.001). Both methods demonstrated 100% specificity, with LCBA-IF showing slightly higher sensitivity compared to FCBA-IF. Within LCBAIF-tested groups, statistically significant differences in annualized relapse rates were observed across all pairwise comparisons (low-titer vs. moderate-titer, low-titer vs. high-titer, and moderate-titer vs. high-titer; all p<0.050). However, this association reached statistical significance in some FCBA-IF-tested groups. Conclusions Overall, there is a strong concordance between LCBA-IF and FCBA-IF in detecting AQP4-IgG, where LCBA-IF shows slightly higher sensitivity. Furthermore, there is a potential link between elevated AQP4-IgG titers and an increased risk of relapse, and this correlation may appear more pronounced when using LCBA-IF.

Objective:To evaluate the effectiveness of the new operation mechanism of public hospitals in Sanming, so as to provide a theoretical basis for the sustainable development of the new operation mechanism for public hospitals.Methods:The data on medical income and patient costs of public hospitals in Sanming and the whole country from 2011 to 2021 were selected, structural variation analysis was used to analyze and compare the changes in the structure of medical income of public hospitals in Sanming City and the whole country, and descriptive analysis was carried out on the patient burden costs, in order to validate the effect of the new operation mechanism for public hospitals in Sanming.Results:From 2012 to 2021, the cumulative values of structure variation in medical service revenue and drug/consumable revenue of public hospitals in Sanming City were 29.58% and -32.29% respectively, better than the national figures of -0.95% and -0.68%; the annual contribution rate of structural variation in the medical service revenue and drug/consumable revenue of public hospitals in Sanming were 78.10% on average, higher than the national figure of 49.43%. From 2011 to 2021, the average increase in the average outpatient and emergency care costs per patient in public hospitals in Sanming was 4.35%, lower than the national average of 5.97%; the average increase in the average inpatient costs per patient was 3.91%, lower than the national average of 5.40%.Conclusions:The new mechanism for the operation of public hospitals in Sanming is effective and sustainable under certain policy conditions.

The rapid development of urbanization has led to the realistic demand for the reconstruction of rural medical and health service system. This paper systematically reviewed the rise and changes of rural doctor system, selected Sanming as the experimental sample for reconstruction, and extracted and summarized the " sharing rural doctor" mode. This mode is suitable for administrative villages with a small population under urbanization development. After focusing on service accessibility(service radius, transportation convenience), adjacent administrative villages share village doctors. At the same time, township health centers extend their services through regular visits, dispatching, and other forms to ensure the quality of basic medical and health services. It was found that the key factor of reconstruction rural medical and health service system lied in the three-dimensional reconstruction of goal, structure and form, and the core procedure of reconstruction was to establish the service delivery system of prevention, treatment, health and management. According to the experimental results, this mode has achieved performance improvement at both individual and organizational levels. The mode of Sanming has great reference value for the high-quality development and service mode reconstruction of rural medical and health service system under the background of urbanization.

A total of 101 honey samples that originated from 20 different unifloral honey and other multifloral honey samples were collected from China.FT-NIR spectrometer were applied to determinate the content of fructose and glucose of honey with two different modes: transflectance (800-2500 nm, 2 mm optical path length) and transmittance (800-1370 nm, 20 mm optical path length).It was found that the prediction accuracy of fructose and glucose had significant difference with the two modes.In order to analyze the reason of this difference, support vector machine (SVM) was used to analyze the non-linear information, and genetic algorithm (GA) was used to analyze the characteristic wavelengths.The result indicated that the detection difference of fructose and glucose was originated from their different characteristic wavelengths.Through the optimization of detection method, it was found that for the determination of glucose, short wavelength and long optical path length should be used, on the other side, the whole wavelength region and short wavelength, with selecting the characteristic wavelength to avoid the disturb of water can also be used.For the determination of fructose, whole wavelength region and short optical path length should be used.Linear regression methods such as PLSR could obtain good results, and non-linear methods such as SVM did not improve the model performance.

Objective To observe the inhibitory effect of the antibodies against midgut-protein-ingredient of Anopheles stephensi on the oocysts of Plasmodium yoelii.Methods Female An.stephensi mosquitoes raised in laboratory were dissected and the midguts were collected.Eight BALB/c mice were immunized using midgut-protein(100 ?g/mouse,4 times with an interval of 7～10day).Ten days after the last immunization,blood was taken from mice armpit artery and serum separated.The immune active antigen of the midgut protein was analyzed by Western blotting.Protein with Mr 38 000～50 000 was separated by sephadex filtering and used to immunize 12 BALB/c mice(100 ?g/mouse,4 times with interval of 7～10 days).PBS control group was established.Seven days after the last immunization,serum antibody was detected by ELISA.When the antibody titer in immunized mice reached ≥1:2 560,mice in both groups were infected by P.yoelii(about 2?107 plasmodium-infected RBC) by abdominal injection.The mosquitoes were fed on the infected mice when the number of female gametes was higher than 2 per 10 microscopical fields 3 days later.After 9 days,the mosquitoes were dissected and the amount of oocysts in midgut was counted.Results Eight protein bands were shown in midgut-protein of An.stephensi by Western blotting and the band of Mr 38 000～50 000-midgut-protein appeared clearer.The infection rate of oocysts in the experiment and control groups were 28.70%(62/216) and 51.09%(47/92) respectively(P