The morphological variations of lymphoma cells are diverse,serving as a crucial basis for pathologists to diagnose and classify different types of lymphoma.Morphology represents the external manifestations,while genetic var-iation is one of the key factors determining these morphological changes.Some morphological alterations often hint at the underlying patterns of genetic variation.Unveiling the connections between these morphological features and genetic variations can offer novel insights for lymphoma pathological diagnosis,prediction of biological behavior,and treatment selection.

Objective To explore the role of the brief visuospatial memory test-revised(BVMT-R)in predicting the clinical conversion to psychosis in subjects with clinical high-risk for psychosis(CHR-P).Methods A total of 217 CHR subjects were recruited and assessed using BVMT-R at baseline.Participants were followed up for three years to determine whether they converted to psychosis.The relationship between BVMT-R total score and CHR-P conversion probability was analyzed using generalized additive model,and the cutoff values of BVMT-R total score for predicting CHR-P conversion were calculated by maximally selected rank statistics.Then,the total BVMT-R score was stratified into different intervals based on the cutoff values obtained as previously described.Finally,the positive likelihood ratios and the conversion rates at different time points were calculated for each interval.Results A total of 168 subjects with CHR-P completed the 3-year follow-up assessment.According to the results of the generalized additive model,the relationship between the BVMT-R total score and CHR-P conversion probability exhibited the characteristics of a piecewise function model.The cutoff values identified using the maximally selected rank statistics method were 18 and 29,which divided the BVMT-R total scores into three intervals:0-18,19-29,and 30-36.The positive likelihood ratios of the three intervals for predicting CHR-P psychosis conversion were significantly different(all P<0.01).All three intervals had significantly different rates of psychosis conversion at different follow-up time points(all P<0.01).Conclusion The total BVMT-R score can be divided into three intervals,each associated with a distinct positive likelihood ratio for predicting psychosis conversion in CHR-P individuals.Accordingly,the BVMT-R total score may serve as a preliminary indicator for estimating the probability of psychosis conversion in the CHR-P population.

The morphological variations of lymphoma cells are diverse,serving as a crucial basis for pathologists to diagnose and classify different types of lymphoma.Morphology represents the external manifestations,while genetic var-iation is one of the key factors determining these morphological changes.Some morphological alterations often hint at the underlying patterns of genetic variation.Unveiling the connections between these morphological features and genetic variations can offer novel insights for lymphoma pathological diagnosis,prediction of biological behavior,and treatment selection.

Objective To explore the role of the brief visuospatial memory test-revised(BVMT-R)in predicting the clinical conversion to psychosis in subjects with clinical high-risk for psychosis(CHR-P).Methods A total of 217 CHR subjects were recruited and assessed using BVMT-R at baseline.Participants were followed up for three years to determine whether they converted to psychosis.The relationship between BVMT-R total score and CHR-P conversion probability was analyzed using generalized additive model,and the cutoff values of BVMT-R total score for predicting CHR-P conversion were calculated by maximally selected rank statistics.Then,the total BVMT-R score was stratified into different intervals based on the cutoff values obtained as previously described.Finally,the positive likelihood ratios and the conversion rates at different time points were calculated for each interval.Results A total of 168 subjects with CHR-P completed the 3-year follow-up assessment.According to the results of the generalized additive model,the relationship between the BVMT-R total score and CHR-P conversion probability exhibited the characteristics of a piecewise function model.The cutoff values identified using the maximally selected rank statistics method were 18 and 29,which divided the BVMT-R total scores into three intervals:0-18,19-29,and 30-36.The positive likelihood ratios of the three intervals for predicting CHR-P psychosis conversion were significantly different(all P<0.01).All three intervals had significantly different rates of psychosis conversion at different follow-up time points(all P<0.01).Conclusion The total BVMT-R score can be divided into three intervals,each associated with a distinct positive likelihood ratio for predicting psychosis conversion in CHR-P individuals.Accordingly,the BVMT-R total score may serve as a preliminary indicator for estimating the probability of psychosis conversion in the CHR-P population.

In the past decade, significant progress has been made in the field of haematolymphoid pathology in China. Domestic experts have published nearly 100 articles in the Chinese Journal of Pathology and international authoritative journals. These achievements have deepened the fundamental theoretical research of haematolymphoid pathology and significantly improved the clinical pathological diagnosis level, providing valuable Chinese data for the pathological diagnosis and research of haematolymphoid diseases internationally. In addition, the domestic haematolymphoid pathological diagnosis and training system has been comprehensively optimized, with closer interaction and integration with clinical medicine, cultivating a large number of specialized pathologists, and driving innovation and development of the entire pathology discipline.

In the past decade, significant progress has been made in the field of haematolymphoid pathology in China. Domestic experts have published nearly 100 articles in the Chinese Journal of Pathology and international authoritative journals. These achievements have deepened the fundamental theoretical research of haematolymphoid pathology and significantly improved the clinical pathological diagnosis level, providing valuable Chinese data for the pathological diagnosis and research of haematolymphoid diseases internationally. In addition, the domestic haematolymphoid pathological diagnosis and training system has been comprehensively optimized, with closer interaction and integration with clinical medicine, cultivating a large number of specialized pathologists, and driving innovation and development of the entire pathology discipline.

Objective:To evaluate the effect of the positive P-glycoprotein expression on the efficacy of patient-controlled intravenous analgesia (PCIA) with sufentanil or pentazocine in patients with cancer pain.Methods:This was a retrospective cohort study. The medical records of patients with cancer pain of either sex, aged 54-71 yr, weighing 49-67 kg, with TNM stage Ⅱ-Ⅳ, who were treated in People′s Hospital of Lishui from January 2020 to January 2024, were collected. The expression of P-glycoprotein in tumor tissues was determined by the immunohistochemical method. Patients with negative P-glycoprotein expression in tumor tissues were divided into 2 groups: sufentanil group (group S 1) and pentazocine group (group P 1). Patients with positive P-glycoprotein expression in tumor tissues were divided into 2 groups: sufentanil group (group S 2) and pentazocine group (group P 2). The patients in 4 groups received 48 h of PCIA when visual analogue scale > 5 cm. The PCIA solution contained sufentanil 2 μg/kg and tropisetron 10 mg in 100 ml of normal saline in S 1 and S 2 groups or pentazocine 3 mg/kg+ tropisetron 10 mg in 100 ml of normal saline in P 1 and P 2 groups. The PCIA pump was set up to deliver a 1 ml bolus dose with a 10-min lockout interval and background infusion at 2 ml/h after a loading dose of 5 ml. Flurbiprofen 50 mg was intravenously injected when visual analogue scale > 3 cm during analgesia. The consumption of sufentanil, pentazocine and flurbiprofen within 4 h, >4-12 h, > 12-24 h and > 24-48 h of PCIA was recorded. The occurrence of adverse reactions such as respiratory depression (SpO 2<90%), nausea or/and vomiting, pruritus and bradycardia was recorded. Results:One hundred patients were finally included, with 25 in each group. There was no significant difference in the consumption of sufentanil, usage rate of flurbiprofen and incidence of respiratory depression, nausea and vomiting, pruritus and bradycardia during analgesia at each time period during PCIA between group S 1 and group S 2 ( P>0.05). Compared with group P 1, the consumption of pentazocinein was significantly increased within 4 h, > 4-12 h, and > 24-48 h of PCIA ( P<0.05), and no significant change was found in the usage rate of flurbiprofen at each time period and the incidence of respiratory depression, nausea or/and vomiting, pruritus and bradycardia during analgesia in group P 2 ( P>0.05). Conclusions:Positive P-glycoprotein expression may weaken the efficacy of PCIA with pentazocine, but exerts no effect on the efficacy of PCIA with sufentanil in patients with cancer pain.

Objective:To explore the clinicopathological characteristics, immunophenotype, diagnosis and differential diagnosis of renal mucinous tubular and spindle cell carcinoma (MTSCC), and to explore the all-exon mutations, microsatellite stability and tumor mutational burden (TMB) in MTSCC cases.Methods:The data of 5 patients with MTSCC that were submitted to the Department of Pathology, First Affiliated Hospital of Soochow University, China from January 2008 to May 2020, were reviewed and analyzed. The whole exome sequencing (WES) was conducted in all patients, while 3 of them were subject to the analyses of microsatellite stability and TMB.Results:Among the 5 patients, 3 were males and 2 were females. They were 37-76 years old. The maximum diameter of the tumor was 3.5-6.0 cm. The borders of the tumors were well defined. Microscopically, MTSCC was characterized by tubular structure, spindle cell and mucinous stroma, and the nuclear grade of tumor cells was overall low. The average follow-up was 15 months, and no recurrence or metastasis was found. Immunohistochemistry showed that all 5 cases were positive for broad-spectrum cytokeratin (CKpan), cytokeratin (CK)7, CK19, vimentin, PAX8, and P504s (varying expression levels), and the Ki-67 positive index was low. The WES of 5 cases showed that NF2 and PTPN14 exhibited higher mutation rates, which were 3/5 and 2/5, respectively. The microsatellite stability analysis indicated that the 3 cases were all microsatellite stable, and the TMB analysis showed that the TMB of the 3 cases were all <9 mut/Mb.Conclusions:MTSCC is a unique, low-grade pleomorphic kidney tumor. The WES analyses suggest that NF2 and PTPN14 have a higher mutation rate, indicating that the occurrence and development of MTSCC may be closely related to the Hippo pathway. The analysis of microsatellite stability indicates that there is no significant relationship between microsatellite stability and MTSCC, and the TMB analysis suggests that MTSCC patients may not benefit from immunotherapy.

Characterization of the polysaccharides and monosaccharides of Bupleurum chinense was undertaken to identify differences in the Bupleurum chinense's sugar profiles, so as to provide a basis for the identification of different varieties. High performance liquid chromatography (HPLC) was used to generate chromatograms of the total polysaccharides of Bupleurum using an Evaporation Light Detector (ELSD), and a monosaccharide chromatogram was generated using a UV-detector (UV) following polysaccharide derivatization. The data were analyzed using SIMCA software and SPSS software to distinguish different varieties of Bupleurum. The results show that the yield of polysaccharides from Bupleurum falcatum is the highest, while the yield of polysaccharides from Bupleurum chinense is the lowest. The polysaccharide spectrum shows that the molecular weights of the polysaccharides in different Bupleurum differ, and their percentages of the total peak area are also different. The four Bupleurum polysaccharides are composed of mannose, glucuronic acid, rhamnose, galacturonic acid, glucose, galactose, and arabinose, but differ in length. The ratio of glucose to arabinose in Bupleurum chinense, Bupleurum scorzonerifolium, Bupleurum falcatum and Bupleurum marginatum var. stenophyllum is: 3.0-4.0, 5.5-7.0, 12.0-17.0, 9.0-12.0. In this study, a sugar profile technique was developed to provide a new method for the identification of different varieties of Bupleurum.

Objective To investigate the brain gray matter atrophy in behavioral variant of frontotemporal dementia (bvFTD) in order to provide an objective basis for early diagnosis. Methods High-resolution 3D structural brain images of 39 probable bvFTD and 46 controls were acquired by 3.0 Tesla MRI scanner. Neuropsychiatric Inventory (NPI), MMSE, Logical Memory Subtest of the Wechsler Memory Scale-Chinese Revision, Consortium to Establish a Registry for Alzheimer''s Disease drawing and WCST were administered to assess cognitive function and psychological and behavioral symptoms. Then voxel-based morphometry and diffeomorphic anatomical registration through exponentiated lie algebra (VBM-DARTEL)was used to compare the structural difference between bvFTD and control groups. Partial correlation analysis was deployed to examine the relationship between the whole brain volume and the total score of the NPI. Results Compared with the control group, bvFTD patients showed abnormalities in most of the neuropsycological tests (all P<0.01), including the higher scores in NPI(Z=-6.545), and the poor performance in global cognitive function(t=-8.621), execution function(t=4.776), episodic memory (t=-7.990)and visuospatial skills(Z=-4.909). In addition, the volume of specific regions, including notably bilateral prefrontal and pre-temporal cortex, insula and subcortical structures, were significantly reduced in bvFTD group (t=4.89-10.95,P<0.05,FWE-corrected). Most prominent atrophy was observed in bilateral caudate nucleus, amygdala, pallidum, putamen, insula, and rectus gyrus. Moreover, the atrophy of left precuneus (cluster size=193 voxels, peak MNI coordinates x=-7.5, y=-66.0, z=67.5) was significantly associated with the total score of NPI (ρ=-0.515,P<0.05,AlphaSim corrected). Conclusions It indicates that the structure of cortico-striatal-thalamic circuits is compromised in bvFTD. The atrophy of bilateral striatum may be most prominent. Besides, the volume of left precuneus may be associated with the severity of psychological and behavioral symptoms. These findings suggest the atrophy of bilateral striatum and left precuneus might be considered as potential imaging markers of bvFTD.