BACKGROUND:Pain is the main symptom of knee osteoarthritis,which seriously affects the quality of life of elderly patients.The pain mechanism of knee osteoarthritis is complex and involves multi-tissue and multi-discipline.Visual analysis is needed to understand its cutting-edge content and research hotspots.OBJECTIVE:To analyze and summarize the cutting-edge content and research hotspot in knee osteoarthritis pain field using bibliometrics visualization software.METHODS:Literatures related to knee osteoarthritis pain from 2014 to 2024 were retrieved from Web of Science Core Collection(WoSCC).Three visualization software,Citespace,VOSviewer,and Bibliometrix R-Package were used for bibliometric and visual analysis.RESULTS AND CONCLUSION:(1)The number of articles on knee osteoarthritis pain research is increasing year by year.(2)The United States,China,and Australia were the top three countries in the number of publications in this field in 10 years.Boston University,University of Sydney,and University of Florida are the top three research institutions.OSTEOARTHRITIS AND CARTILAGE has published the most research articles in this field,and PAIN has been the most cited journal in this field.The author with the most published articles is Fillingim Roger B.The active authors in this field have formed a number of stable research teams among themselves,but there is a lack of international cooperation.(3)Daily management(motor,psychological,and intelligent management),inflammatory mechanism of pain,proteomics,combination medication,central sensitization,and pain regulation of knee osteoarthritis pain patients are the key contents of knee osteoarthritis pain research.Artificial intelligence assistance,extracorporeal shock wave therapy,radiofrequency ablation,nerve cryotherapy,intra-articular injection of blood or cell preparations may be the research frontiers in this field.

BACKGROUND:Pain is the main symptom of knee osteoarthritis,which seriously affects the quality of life of elderly patients.The pain mechanism of knee osteoarthritis is complex and involves multi-tissue and multi-discipline.Visual analysis is needed to understand its cutting-edge content and research hotspots.OBJECTIVE:To analyze and summarize the cutting-edge content and research hotspot in knee osteoarthritis pain field using bibliometrics visualization software.METHODS:Literatures related to knee osteoarthritis pain from 2014 to 2024 were retrieved from Web of Science Core Collection(WoSCC).Three visualization software,Citespace,VOSviewer,and Bibliometrix R-Package were used for bibliometric and visual analysis.RESULTS AND CONCLUSION:(1)The number of articles on knee osteoarthritis pain research is increasing year by year.(2)The United States,China,and Australia were the top three countries in the number of publications in this field in 10 years.Boston University,University of Sydney,and University of Florida are the top three research institutions.OSTEOARTHRITIS AND CARTILAGE has published the most research articles in this field,and PAIN has been the most cited journal in this field.The author with the most published articles is Fillingim Roger B.The active authors in this field have formed a number of stable research teams among themselves,but there is a lack of international cooperation.(3)Daily management(motor,psychological,and intelligent management),inflammatory mechanism of pain,proteomics,combination medication,central sensitization,and pain regulation of knee osteoarthritis pain patients are the key contents of knee osteoarthritis pain research.Artificial intelligence assistance,extracorporeal shock wave therapy,radiofrequency ablation,nerve cryotherapy,intra-articular injection of blood or cell preparations may be the research frontiers in this field.

After sequencing, we amplified and cloned foot-and-mouth disease virus (FMDV) O/QYYS/s/06 whole genome by three fragments. These three fragments were cloned into vector P43 one by one to construct recombinant plasmid P43C, which carried the full-length cDNA of FMDV O/QYYS/s/06. Then, plasmid P43C and plasmid T7 expressing T7 RNA polymerase were co-transfected into BHK-21 cells. After 48 h, we harvested the culture broth from transfected BHK-21 cells and inoculated into 2-3 day-old sucking mice. After four generation passage, the virus harvested from sucking mice was confirmed to be type O FMDV by the indirect hemagglutination test, sucking mice's neutralization test and sequencing. The results showed that we have successfully constructed the full-length cDNA clone of FMDV O/QYYS/s/06 strain.
Animals ; Animals, Newborn ; Cloning, Molecular ; DNA, Complementary ; genetics ; DNA, Viral ; biosynthesis ; genetics ; Foot-and-Mouth Disease ; virology ; Foot-and-Mouth Disease Virus ; classification ; genetics ; pathogenicity ; Mice ; Transcription, Genetic ; Transfection