Objective:To systematically evaluate the measurement properties of foot care behavior assessment tools for patients with diabetes mellitus and the methodological quality of the study, and to inform the selection of a high-quality assessment tool for healthcare professionals.Methods:Two researchers independently searched Chinese and English databases such as CINAHL, Web of Science, Cochrane Library, PubMed, Embase, China National Knowledge Infrastructure, VIP, and Wanfang Data to screen for studies of foot care behavior assessment tools for patients with diabetes mellitus. The search period was from database establishment to June 2024. Based on the Consensus-based Standards for the selection of health Measurement Instruments (COSMIN) systematic review guideline, the risk of bias checklist and quality standard rating scale were used to evaluate the measurement properties and form recommendations.Results:A total of 20 studies were included, comprising 9 diabetes foot care behavior assessment tools, of which 12 were translated versions of the original scales and the remaining 8 were developed and validated as localized scales in each country. None of the studies reported on the cross-cultural validity and responsiveness of the assessment tools.Conclusions:None of the foot care behavior assessment tools for patients with diabetes mellitus included in this study has perfect measurement properties. Compared with other scales, the Chinese Community Diabetic Foot Care Behavior Questionnaire and the Colombian Instrument of Self-Care for Prevention of Diabetic Foot (ISPDF) provide evidence of high-quality psychometric properties and can be recommended for use, but further in-depth validation is needed. There is no evidence of "adequate" content validity and internal consistency for the remaining scales, which are recommended at Level B.

Objective:To systematically evaluate the measurement properties of foot care behavior assessment tools for patients with diabetes mellitus and the methodological quality of the study, and to inform the selection of a high-quality assessment tool for healthcare professionals.Methods:Two researchers independently searched Chinese and English databases such as CINAHL, Web of Science, Cochrane Library, PubMed, Embase, China National Knowledge Infrastructure, VIP, and Wanfang Data to screen for studies of foot care behavior assessment tools for patients with diabetes mellitus. The search period was from database establishment to June 2024. Based on the Consensus-based Standards for the selection of health Measurement Instruments (COSMIN) systematic review guideline, the risk of bias checklist and quality standard rating scale were used to evaluate the measurement properties and form recommendations.Results:A total of 20 studies were included, comprising 9 diabetes foot care behavior assessment tools, of which 12 were translated versions of the original scales and the remaining 8 were developed and validated as localized scales in each country. None of the studies reported on the cross-cultural validity and responsiveness of the assessment tools.Conclusions:None of the foot care behavior assessment tools for patients with diabetes mellitus included in this study has perfect measurement properties. Compared with other scales, the Chinese Community Diabetic Foot Care Behavior Questionnaire and the Colombian Instrument of Self-Care for Prevention of Diabetic Foot (ISPDF) provide evidence of high-quality psychometric properties and can be recommended for use, but further in-depth validation is needed. There is no evidence of "adequate" content validity and internal consistency for the remaining scales, which are recommended at Level B.

Objective:To investigate the correlation between clinical phenotypes and genetic characteristics of children with ring chromosomes (RCs).Methods:Case series study.The clinical data of 11 434 children who received treatment and peripheral blood chromosome karyotype detection in Henan Children′s Hospital from October 2008 to October 2023 due to growth retardation, intellectual impairment or congenital malformation were analyzed retrospectively.A total of 25 children with RCs were selected.Their age at diagnosis, karyotype distribution, clinical manifestations, and genetic detection results were analyzed.Results:RCs were detected in 25 out of 11 434 children, with a detection rate of 0.21%.The genome-wide copy number variation (CNV) analysis was performed on 7 RCs cases, and it found that pathogenic variation existed in all of them.Among the 25 RC cases (11 males and 14 females of social gender), the age at diagnosis ranged from 2 months to 14 years; there were 20 autosomal rings and 5 sex chromosome rings; 13 cases had chimeric karyotypes, and 12 cases had non-chimeric karyotypes.Most of the 25 children showed clinical manifestations of mental or developmental retardation, and some also presented with specific clinical manifestations, such as short stature, congenital malformation, and epilepsy.Conclusions:The pathogenesis of RCs is complex.The clinical manifestations are determined by both RCs syndrome and specific phenotypes caused by the dose effect and exhibit high heterogeneity, so it is easy to miss or misdiagnose.The combined application of cellular and molecular genetic detection technology can facilitate early diagnosis and treatment of RCs, and the correlation analysis of phenotypes and genetic characteristics can provide guidance for genetic counseling.

OBJECTIVE: To assess the value of detecting the rearrangement of mixed lineage leukemia (MLL) gene in children with acute mononuclear leukemia (AML). METHODS: Dual-color fluorescence in situ hybridization (FISH) probe was used to detect MLL gene rearrangement in 68 children with AML by interphase FISH. The results were compared with that of conventional G banding chromosomal analysis. RESULTS: Among the 68 children, 28 were detected by FISH with positive hybridization signals, with a detection rate for MLL gene rearrangement being 41.2%. Twelve (17.6%) reciprocal translocations and interruption of 11q23 were detected by G banding analysis. The difference in the detection rates between the two methods was statistically significant (P< 0.05). CONCLUSION The sensitivity of FISH assay for MLL gene rearrangement was significantly higher than that of G banding chromosomal karyotyping. Combined use of both methods for children with AML can improve the detection rate of MLL gene rearrangements and provide crucial clues for clinical diagnosis, treatment and prognosis.
Child ; Chromosomes, Human, Pair 11 ; Gene Rearrangement ; Histone-Lysine N-Methyltransferase ; genetics ; Humans ; In Situ Hybridization, Fluorescence ; Leukemia, Monocytic, Acute ; genetics ; Myeloid-Lymphoid Leukemia Protein ; genetics ; Translocation, Genetic

Objective To observe the clinical effect of Jianpilishifang combined with magnesium sulfate and calcium antagonists in the treatment of gestational hypertension.Methods 81 cases from January 2014 to June 2015 in gynaecology and obstetrics department of Shengzhou City People’s Hospital were selected and divided into 41 patients in observation group and 40 patients in control group according to a randomized and double-blind method.The control group were treated with magnesium sulfate and calcium antagonist treatment and observation group were treated with Jianpilishifang treatment on the basis of control group.The systolic blood pressure, diastolic blood pressure, 24 h urinary protein (24 hUTP), urinary transferrin ( TRF) , blood viscosity, erythrocyte aggregation index and comparative treatment efficacy were compared pre-and post-treatment.Results The systolic blood pressure and diastolic blood pressure in observation group post-treatment were (125.73 ±5.48, 80.06 ±3.34)mmHg, which were lower than (140.16 ±7.02, 86.79 ±5.21)mmHg in control group (P<0.05).The levels of 24 hUTP and TRF in observation group post-treatment were (1.75 ± 0.14)g/24 h, (1.41 ±0.28)mg/L, which were lower than (2.55 ±0.18)g/24 h, (2.23 ±0.37)mg/L in control group (P<0.05).The blood viscosity, erythrocyte aggregation index in observation group post-treatment were (2.00 ±0.23)mPa? s, (3.11 ±0.20), which were lower than (2.56 ± 0.47)mPa? s, (3.69 ±0.41)in control group (P<0.05).The total effective rate was 95.12% in observation group, which was significantly higher than 80.00% in control group ( P<0.05 ) .Conclusion Jianpilishifang combined with magnesium sulfate and calcium antagonists could effectively reduce blood pressure and improve blood viscosity in the treatment of gestational hypertension.The therapeutic effect of combination therapy is superior to monotherapy.