Objective:To explore the mechanism by which the extract of Hedyotis diffusa Willd regulates TGF-β/Smad signaling transduction pathway and affects proliferation,invasion of renal cancer cells and expressions of Th1/Th2 cytokines in mice with renal cancer.Methods:Using mouse renal cancer Renca cells as the research object,after intervention with extracts of Hedyotis diffusa Willd,impact on migration ability of mouse renal cancer cells was detected at the cellular level using scratch assay;observing effect of CCK-8 experiment on proliferation ability of mouse renal cancer cells.A mouse kidney cancer model was established by subcutaneous inoculation under the right armpit,and effect of extract of Hedyotis diffusa Willd on proliferation and invasion of renal carcinoma in mice was studied.HE staining was used to observe histomorphology of transplanted tumor mass in mice;Western blot was used to detected expressions of TGF-β/Smad pathway protein TGF-β,Smad2 and p-Smad2 in mouse transplanted tumor tissue;levels of IL-2 and IL-10 in mouse serum were detected by ELISA to investigate whether the extract of Hedyotis diffusa Willd has an inhibitory effect on Th1/Th2 renal carcinoma immune shift.Results:CCK-8 experiment showed that the extract of Hedyotis diffusa Willd inhibited pro-liferation ability of mouse renal cancer Renca cells(P<0.05);scratch test results showed that extract of Hedyotis diffusa Willd effec-tively inhibited the healing ability of mouse renal cancer Renca cells(P<0.05);by constructing a mouse kidney cancer model,it was found that extract of Hedyotis diffusa Willd could inhibit the growth of transplanted tumor volume in mice(P<0.05);HE staining revealed that tumor cells in model group were dense,with numerous mitotic figures and minimal necrosis;whereas in Hedyotis diffusa Willd group and pathway inhibitor group,there was increased necrosis of cancer cells,accompanied by karyopyknosis or lysis;Western blot showed that compared with model group,expression of TGF-β and p-Smad2 in extract of Hedyotis diffusa Willd group and pathway inhibitor group were decreased(P<0.001);ELISA results showed that compared with model group,serum IL-2 expression increased in both Hedyotis diffusa Willd group and pathway inhibitor group(P<0.000 1).IL-10 expression decreased in Hedyotis diffusa Willd group(P<0.05)and pathway inhibitor group(P>0.05).Conclusion:Extract of Hedyotis diffusa Willd may inhibit protein expressions in TGF-β/Smads signaling pathway,further regulate the secretion of Th1/Th2 cytokines,thereby inhibiting proliferation and migration of mouse renal cancer cells both in vitro and in vivo.

Objective:To explore the mechanism by which the extract of Hedyotis diffusa Willd regulates TGF-β/Smad signaling transduction pathway and affects proliferation,invasion of renal cancer cells and expressions of Th1/Th2 cytokines in mice with renal cancer.Methods:Using mouse renal cancer Renca cells as the research object,after intervention with extracts of Hedyotis diffusa Willd,impact on migration ability of mouse renal cancer cells was detected at the cellular level using scratch assay;observing effect of CCK-8 experiment on proliferation ability of mouse renal cancer cells.A mouse kidney cancer model was established by subcutaneous inoculation under the right armpit,and effect of extract of Hedyotis diffusa Willd on proliferation and invasion of renal carcinoma in mice was studied.HE staining was used to observe histomorphology of transplanted tumor mass in mice;Western blot was used to detected expressions of TGF-β/Smad pathway protein TGF-β,Smad2 and p-Smad2 in mouse transplanted tumor tissue;levels of IL-2 and IL-10 in mouse serum were detected by ELISA to investigate whether the extract of Hedyotis diffusa Willd has an inhibitory effect on Th1/Th2 renal carcinoma immune shift.Results:CCK-8 experiment showed that the extract of Hedyotis diffusa Willd inhibited pro-liferation ability of mouse renal cancer Renca cells(P<0.05);scratch test results showed that extract of Hedyotis diffusa Willd effec-tively inhibited the healing ability of mouse renal cancer Renca cells(P<0.05);by constructing a mouse kidney cancer model,it was found that extract of Hedyotis diffusa Willd could inhibit the growth of transplanted tumor volume in mice(P<0.05);HE staining revealed that tumor cells in model group were dense,with numerous mitotic figures and minimal necrosis;whereas in Hedyotis diffusa Willd group and pathway inhibitor group,there was increased necrosis of cancer cells,accompanied by karyopyknosis or lysis;Western blot showed that compared with model group,expression of TGF-β and p-Smad2 in extract of Hedyotis diffusa Willd group and pathway inhibitor group were decreased(P<0.001);ELISA results showed that compared with model group,serum IL-2 expression increased in both Hedyotis diffusa Willd group and pathway inhibitor group(P<0.000 1).IL-10 expression decreased in Hedyotis diffusa Willd group(P<0.05)and pathway inhibitor group(P>0.05).Conclusion:Extract of Hedyotis diffusa Willd may inhibit protein expressions in TGF-β/Smads signaling pathway,further regulate the secretion of Th1/Th2 cytokines,thereby inhibiting proliferation and migration of mouse renal cancer cells both in vitro and in vivo.

Objective To investigate the effect of Xuebijing injection on the inflammatory and coagulation response of complicated intra-abdominal infection by sepsis.Methods A retrospective study was conducted.A total of 274 patients with complicated intra-abdominal infection by sepsis admitted to Yidu Central Hospital of Weifang from June 2020 to July 2023 were enrolled.According to whether Xuebijing was used or not,the patients were divided into an observation group(141 cases)and a control group(133 cases).The patients in both groups were treated with conventional western medicine,and those in the observation group were additionally given Xuebijing(Xuebijing injection 100 mL in 0.9%NaCl solution 100 mL via intravenous infusion,twice per day for a continuous application of 5 days).The differences of clinical prognosis and the serum levels of inflammatory indicators[interleukin-6(IL-6),procalcitonin(PCT),C-reactive protein(CRP)and endotoxin]and coagulation indexes[D-dimer,fibrinogen(FIB),prothrombin time(PT)and activated partial thromboplastin time(APTT)before treatment and on the 2nd,3rd,5th day respectively after treatment were compared,and the clinical prognosis was observed.Results There were no significant differences in the indexes of inflammation and coagulation function between the two groups before treatment.With the extension of therapy duration,following treatment in two groups,the serum levels of inflammatory indicators and coagulation indexes(D-dimer,FIB)decreased,as did coagulation indexes(PT,APTT),reaching the lowest levels on the 5th day.The inflammatory markers and coagulation indexes of observation group were significantly lower than those of control group[IL-6(ng/L):18.89±8.22 vs.24.59±10.39,PCT(μg/L):0.30±0.16 vs.0.52±0.20,CRP(mg/L):13.13±6.54 vs.28.87±8.66,endotoxin(kEU/L):0.17±0.06 vs.0.22±0.08,D-dimer(mg/L):1.75±0.61 vs.1.96±0.77,FIB(g/L):2.50±0.78 vs.3.10±0.94,PT(s):14.16±1.90 vs.15.05±1.46,APTT(s):34.52±5.40 vs.36.21±4.72,both P<0.05].The intensive care unit(ICU)stay time and total hospitalization time were shortened in the observation group than those in the control group[ICU stay time(days):5.71±2.22 vs.6.86±2.99,hospitalization time(days):15.50±6.39 vs.17.02±5.70,both P<0.05];the mortality was significantly lower in the observation group than that in the control group[21.27%(30/141)vs.32.33%(43/133),P<0.05].Conclusion The treatment of complicated intra-abdominal infection by sepsis with Xuebijing injection while receiving conventional western medicine treatment can effectively decrease inflammatory and coagulation response,have high efficacy of inflammatory and coagulation co-treatment,and have some important clinical values.