Objective:To conduct consistency analysis of antinuclear antibody (ANA) anti-cell type 2 (AC-2) fluorescence pattern based on HEp-2 cells and monkey liver matrix, and to investigate the clinical significance of the AC-2 and anti-dense fine speckled 70 (DFS70) antibody.Methods:This study is a cross-sectional study. Retrospective analysis of the results of 33 921 ANA tests detected by indirect immunofluorescence assay (IIFA) from January 2024 to December 2024 in Shengjing Hospital of China Medical University. A total of 457 patients with AC-2 positive were taken from the cohort as the research objectives, then the positive rate of AC-2 in ANA among the 457 patients and its distribution in different diseases were counted. One hundred cases were selected from 457 AC-2 positive cases, whose AC-2 fluorescence pattern were analyzed using HEp-2 cells and HEp-2 cells combined with monkey liver matrix and the anti-DFS70 antibody was detected to help identifying the AC-2 fluorescence pattern. Finally, the consistency of the two methods in identifying the AC-2 fluorescence pattern was compared and the clinical significance of the AC-2 and anti-DFS70 antibody was discussed. Spearman correlation analysis was used to compare the correlation between the reciprocals of ANA titer of AC-2 positive samples and the levels of anti-DFS70 antibody.Results:AC-2 positive cases accounted for 1.15% (457/33 921) and 3.64% (457/12 555) of the all cases and ANA positive cases, respectively. The positive rate of AC-2 in females (394/457) was higher than that in males (63/457), P<0.01. Totally 87.09% of the AC-2 positive patients aged 40 and below. Moreover, the most positive cases of AC-2 occured in female infertility, adverse pregnancy history, pregnancy status and patients with connective tissue disease or joint pain/arthritis, accounting for 57.33% of the total positive cases. AC-2 positive patients were mostly distributed in women with infertility, bad pregnancy history, pregnancy status, connective tissue disease or patients manifested with joint pain/arthritis, accounting for 57.33% (262/457) of the total positive cases.The consistency analysis of AC-2 fluorescence pattern based on HEp-2 cells and HEp-2 cells combined with monkey liver matrix showed that among 100 serum samples, 97 cases were unanimously determined to be AC-2 fluorescence pattern, suggesting that HEp-2 cells can identify most AC-2 fluorescent pattern.The other three cases were identified as AC-1 and AC-5, and these AC-2 miscible pattern could be identified by monkey liver matrix and DFS70 antibody. In addition, the positive rate of anti-DFS70 antibody was 92.78% (90/97) in AC-2 fluorescent pattern, and the titer of ANA was positively correlated with the concentration of anti-DFS70 antibody in 62 cases with single positive anti-DFS70 antibody ( r=0.441, P<0.05). Among 62 cases of single positive anti-DFS70 antibody, 5 patients with connective tissue disease and 57 patients with non-autoimmune diseases; among the 25 cases of positive DFS70 antibody combined with non-positive ANA spectrum antibody, 4 cases were pregnant, and 16 cases had infertility or bad pregnancy history. All of them were combined with anti-thyroid globulin or thyroid peroxidase antibody positive, lupus anticoagulant positive, phospholipid antibody and herpes virus, rubella virus or cytomegalovirus antibody positive. Two cases were arthritis patients with positive anti-cyclic citrulline colypeptide antibody. One patient was Hashimoto′s thyroid, and 1 case was antiphospholipid syndrome. Three autoimmune diseased patients with positive DFS70 antibody were combined with positive ANA spectrum antibody, which were anti-Sjogren′s syndrome A antigen, polymyositis-scleroderma antigen antibody. Conclusion:Most AC-2 fluorescent pattern can be identified by HEp-2 cells, monkey liver matrix and anti-DFS70 antibody are helpful to identify AC-2, AC-2 fluorescent pattern and DFS70 antibodies can be positive alone or combined with ANA spectrum antibodies and other non-ANA spectrum antibodies in different diseases, which account for a high proportion of female infertility patients, patients with bad pregnancy history and pregnancy status.

Objective:To conduct consistency analysis of antinuclear antibody (ANA) anti-cell type 2 (AC-2) fluorescence pattern based on HEp-2 cells and monkey liver matrix, and to investigate the clinical significance of the AC-2 and anti-dense fine speckled 70 (DFS70) antibody.Methods:This study is a cross-sectional study. Retrospective analysis of the results of 33 921 ANA tests detected by indirect immunofluorescence assay (IIFA) from January 2024 to December 2024 in Shengjing Hospital of China Medical University. A total of 457 patients with AC-2 positive were taken from the cohort as the research objectives, then the positive rate of AC-2 in ANA among the 457 patients and its distribution in different diseases were counted. One hundred cases were selected from 457 AC-2 positive cases, whose AC-2 fluorescence pattern were analyzed using HEp-2 cells and HEp-2 cells combined with monkey liver matrix and the anti-DFS70 antibody was detected to help identifying the AC-2 fluorescence pattern. Finally, the consistency of the two methods in identifying the AC-2 fluorescence pattern was compared and the clinical significance of the AC-2 and anti-DFS70 antibody was discussed. Spearman correlation analysis was used to compare the correlation between the reciprocals of ANA titer of AC-2 positive samples and the levels of anti-DFS70 antibody.Results:AC-2 positive cases accounted for 1.15% (457/33 921) and 3.64% (457/12 555) of the all cases and ANA positive cases, respectively. The positive rate of AC-2 in females (394/457) was higher than that in males (63/457), P<0.01. Totally 87.09% of the AC-2 positive patients aged 40 and below. Moreover, the most positive cases of AC-2 occured in female infertility, adverse pregnancy history, pregnancy status and patients with connective tissue disease or joint pain/arthritis, accounting for 57.33% of the total positive cases. AC-2 positive patients were mostly distributed in women with infertility, bad pregnancy history, pregnancy status, connective tissue disease or patients manifested with joint pain/arthritis, accounting for 57.33% (262/457) of the total positive cases.The consistency analysis of AC-2 fluorescence pattern based on HEp-2 cells and HEp-2 cells combined with monkey liver matrix showed that among 100 serum samples, 97 cases were unanimously determined to be AC-2 fluorescence pattern, suggesting that HEp-2 cells can identify most AC-2 fluorescent pattern.The other three cases were identified as AC-1 and AC-5, and these AC-2 miscible pattern could be identified by monkey liver matrix and DFS70 antibody. In addition, the positive rate of anti-DFS70 antibody was 92.78% (90/97) in AC-2 fluorescent pattern, and the titer of ANA was positively correlated with the concentration of anti-DFS70 antibody in 62 cases with single positive anti-DFS70 antibody ( r=0.441, P<0.05). Among 62 cases of single positive anti-DFS70 antibody, 5 patients with connective tissue disease and 57 patients with non-autoimmune diseases; among the 25 cases of positive DFS70 antibody combined with non-positive ANA spectrum antibody, 4 cases were pregnant, and 16 cases had infertility or bad pregnancy history. All of them were combined with anti-thyroid globulin or thyroid peroxidase antibody positive, lupus anticoagulant positive, phospholipid antibody and herpes virus, rubella virus or cytomegalovirus antibody positive. Two cases were arthritis patients with positive anti-cyclic citrulline colypeptide antibody. One patient was Hashimoto′s thyroid, and 1 case was antiphospholipid syndrome. Three autoimmune diseased patients with positive DFS70 antibody were combined with positive ANA spectrum antibody, which were anti-Sjogren′s syndrome A antigen, polymyositis-scleroderma antigen antibody. Conclusion:Most AC-2 fluorescent pattern can be identified by HEp-2 cells, monkey liver matrix and anti-DFS70 antibody are helpful to identify AC-2, AC-2 fluorescent pattern and DFS70 antibodies can be positive alone or combined with ANA spectrum antibodies and other non-ANA spectrum antibodies in different diseases, which account for a high proportion of female infertility patients, patients with bad pregnancy history and pregnancy status.

Objective:To investigate the current implementation status of early essential newborn care (EENC) core interventions in China.Methods:Based on the Safe Neonatal Project jointly launched by the National Health Commission and United Nations International Children's Emergency Fund (UNICEF), a questionnaire survey was conducted on the implementation of eight EENC core interventions in 161 pilot hospitals. The eight interventions were skin-to-skin contact immediately after birth for at least 90 min, delayed cord clamping (until 1 min after birth or after the cord stopped pulsating), initiation of breastfeeding within the first hour after birth, exclusive breastfeeding at discharge, delayed first bath (24 h after birth), kangaroo mother care, eye care (using erythromycin ointment or other eye drops), and intramuscular injection of vitamin K 1. The survey was completed by the pilot units based on actual conditions from January 1 to December 31, 2022. Neonatal disease information was obtained from medical records from birth to discharge, and neonatal mortality rates were obtained from outpatient and inpatient records in obstetric institutions. The main indicators were the implementation rates of EENC core interventions in the eastern, central, and western regions, as well as the neonatal mortality and the incidence of common neonatal diseases in hospitals that implemented EENC or not. Chi-square test was used for data comparison. Results:A total of 156 pilot hospitals completed the survey, with 66 (42.3%) in the eastern region, 45 (28.8%) in the central region, and 45 (28.8%) in the western region. The implementation rates of skin-to-skin contact immediately after birth for at least 90 min, initiation of breastfeeding within the first hour after birth, exclusive breastfeeding at discharge, kangaroo mother care, eye care, and intramuscular injection of vitamin K 1 were higher in the western region than in the eastern and central regions [60.1% (62 491/104 012) vs. 22.4% (104 991/467 982) and 29.4% (31 419/106 812), 76.5% (78 188/102 266) vs. 41.2% (188 740/458 627) and 62.9% (67 200/106 812), 74.0% (60 062/81 141) vs. 39.7% (181 921/458 627) and 70.2% (74 947/106 812), 27.9% (1 407/5 035) vs. 9.4% (1 670/17 807) and 22.9% (2 510/10 968), 46.9% (48 442/103 313) vs. 23.0% (105 917/461 176) and 31.4% (31 731/100 966), 96.2% (51 674/53 729) vs. 56.6% (263 273/465 433) and 92.1% (98 365/106 812), χ 2 values were 58 218.40, 50 113.04, 55 466.24, 1 456.92, 24 686.13, and 72 696.66, respectively, all P<0.001]. The implementation rates of delayed cord clamping and delayed first bath were higher in the central region than in the eastern and western regions [76.9% (82 161/106 812) vs. 37.0% (163 808/443 285) and 59.3% (60 596/102 266), 75.0% (80 159/106 812) vs. 46.6% (216 935/465 433) and 68.4% (71 161/104 012), χ 2 values were 62 516.10 and 37 987.11, respectively, both P<0.001]. Seventy-six hospitals (48.7%) implemented EENC, while 80 hospitals (51.3%) did not. The neonatal mortality and the incidence of neonatal asphyxia, sepsis, pneumonia, diarrhea, eye infection, and umbilical cord infection were higher in the hospitals that did not implement EENC than in those that did [1.3‰ (478/378 519) vs. 1.0‰ (645/679 795), 1.8% (6 849/378 519) vs. 0.9% (5 895/679 795), 1.0% (3 917/378 519) vs. 0.7% (5 029/679 795), 6.2% (23 481/377 890) vs. 3.8% (25 463/679 795), 0.2% (916/378 519) vs. 0.1% (950/656 781), 0.5% (1 837/370 540) vs. 0.2% (1 403/656 949), 1.1‰ (422/375 149) vs. 0.6‰ (405/672 291), χ 2 values were 22.62, 1 766.11, 184.86, 2 832.09, 100.89, 866.59, and 64.94, respectively, all P<0.001]. Conclusions:About half of the pilot hospitals did not carry out EENC and the implementation of specific measures in hospitals carrying out EENC still needs to be improved. The implementation rate of EENC core measures is high in some regions, but there are regional differences.Neonatal health outcomes were better in hospitals with EENC than in hospitals without EENC.

Objective:To investigate the self-defect sensation and its influencing factors in patients scheduled for photodynamic therapy for port wine stains.Methods:A total of 183 patients scheduled for photodynamic therapy for port wine stains in our hospital were selected and investigated by online questionnaire. The data of the respondents were collected by means of online filling in the questionnaire star using the General Data Survey Scale and the Defect Perception Scale (FIS) at admission.Results:The total score of self-defect in 183 patients with bright red nevus treated by photodynamic therapy was higher than that of the domestic norm. Age ( t=4.19), place of residence ( F=14.90), co-resident ( F=3.21), education level ( F=4.39), marital status ( F=2.47), income level ( F=9.51) and occupation ( F=5.82) had significantly related with the level of self-deficiency of patients ( P<0.05). The results of multiple linear regression analysis indicated that residence and income level were the main factors affecting patients′ sense of self-deficiency ( F=4.96, P<0.05). Conclusions:The overall self-defect sense of 183 patients with port wine stains treated with photodynamic therapy in this survey is more serious, their own comprehensive conditions and family structure will affect the self-defect sense of patients with port wine stains. We should pay special attention to the mental health of patients from municipal cities and low-income levels, timely carry out clinical nursing education and psychological guidance, jointly improve the compliance and cooperation of patients with treatment, and promote the rapid recovery of patients.

Objective:To explore theepidemiological characteristics and analyse the survival of pneumoconiosis in Chengdu from 2011 to 2021, providing scientific evidence for further prevention and control.Methods:In April 2022, the pneumoconiosis report card of Chengdu from 2012 to 2021 and survival data were collected from the China Disease Control and prevention information system and the occupational pneumoconiosis follow-up survey project.The data of the report card was reorganized and analyzed by R4.4.0 software. Chi-square test was used to compare the case composition ratio between groups. The annual trend of the number of new pneumo coniosis cases was analyzed by linear regression model Kaplan-Meier method was used for survival analysis and log-rank test was used for comparison of survival curves between groups.Results:816 cases of pneumoconiosis were reported in Chengdu from 2011 to 2021, including 522 cases of stage Ⅰ (63.97%, 522/816), 148 cases of stage Ⅱ (18.14%, 148/816) and 146 cases of stage Ⅲ pneumoconiosis (17.89146/816) ; There were 596 cases of silicosis (73.04%, 596/816), 143 cases of coal worker's pneumoconiosis (17.52%, 143/816). New onset pneumoconiosis was mainly male (810 cases, 99.26%). The median age of diagnosis and the 25th and 75th percentile were 63 (51-71) years old, the median length of dust exposure were 12.00 (5.92-28.00) years, and the types of work were mainly rock drillers (24.63%, 201/816), tunneling workers (19.36%, 158/816) and coal miners (13.60%, 111/816). Among 816 cases of new pneumoconiosis, 35 cases were lost to follow-up (4.29%, 35/816), 605 cases survived and 176 cases died. The 5-year survival rate was 78.6% and the 10-year survival rate was 65.8%.Conclusion:The number of pneumoconiosis reported in Chengdu was relatively stable in recent years. The main type of pneumoconiosis was silicosis, which was concentrated in regions, industries and types of work. We should continue to strengthen the prevention and treatment of pneumoconiosis.

Objective:To explore theepidemiological characteristics and analyse the survival of pneumoconiosis in Chengdu from 2011 to 2021, providing scientific evidence for further prevention and control.Methods:In April 2022, the pneumoconiosis report card of Chengdu from 2012 to 2021 and survival data were collected from the China Disease Control and prevention information system and the occupational pneumoconiosis follow-up survey project.The data of the report card was reorganized and analyzed by R4.4.0 software. Chi-square test was used to compare the case composition ratio between groups. The annual trend of the number of new pneumo coniosis cases was analyzed by linear regression model Kaplan-Meier method was used for survival analysis and log-rank test was used for comparison of survival curves between groups.Results:816 cases of pneumoconiosis were reported in Chengdu from 2011 to 2021, including 522 cases of stage Ⅰ (63.97%, 522/816), 148 cases of stage Ⅱ (18.14%, 148/816) and 146 cases of stage Ⅲ pneumoconiosis (17.89146/816) ; There were 596 cases of silicosis (73.04%, 596/816), 143 cases of coal worker's pneumoconiosis (17.52%, 143/816). New onset pneumoconiosis was mainly male (810 cases, 99.26%). The median age of diagnosis and the 25th and 75th percentile were 63 (51-71) years old, the median length of dust exposure were 12.00 (5.92-28.00) years, and the types of work were mainly rock drillers (24.63%, 201/816), tunneling workers (19.36%, 158/816) and coal miners (13.60%, 111/816). Among 816 cases of new pneumoconiosis, 35 cases were lost to follow-up (4.29%, 35/816), 605 cases survived and 176 cases died. The 5-year survival rate was 78.6% and the 10-year survival rate was 65.8%.Conclusion:The number of pneumoconiosis reported in Chengdu was relatively stable in recent years. The main type of pneumoconiosis was silicosis, which was concentrated in regions, industries and types of work. We should continue to strengthen the prevention and treatment of pneumoconiosis.

Multi-gene assays have emerged as crucial tools for risk stratification in early-stage breast cancer. This study aimed to evaluate the prognostic significance of the 21-gene recurrence score (RS) in Chinese patients with pN0-1, estrogen receptor-positive (ER
Biomarkers, Tumor/genetics* ; Breast Neoplasms/pathology* ; Female ; Humans ; Neoplasm Recurrence, Local/pathology* ; Neoplasm Staging ; Prognosis ; Receptor, ErbB-2/genetics* ; Receptors, Estrogen

Coronavirus disease 2019(COVID-19),which is caused by SARS-CoV-2,varies with regard to symptoms and mortality rates among populations.Humoral immunity plays critical roles in SARS-CoV-2 infection and recovery from COVID-19.However,differences in immune responses and clinical features among COVID-19 patients remain largely unknown.Here,we report a database for COVID-19-specific IgG/IgM immune responses and clinical parameters(named COVID-ONE-hi).COVID-ONE-hi is based on the data that contain the IgG/IgM responses to 24 full-length/truncated proteins corresponding to 20 of 28 known SARS-CoV-2 proteins and 199 spike protein peptides against 2360 serum samples collected from 783 COVID-19 patients.In addition,96 clinical parameters for the 2360 serum samples and basic information for the 783 patients are integrated into the database.Furthermore,COVID-ONE-hi provides a dashboard for defining samples and a one-click analysis pipeline for a single group or paired groups.A set of samples of interest is easily defined by adjusting the scale bars of a variety of parameters.After the"START"button is clicked,one can readily obtain a comprehensive analysis report for further interpretation.COVID-ONE-hi is freely available at www.COVID-ONE.cn.

Objective: Based on the information-motivation-behavioral skills (IMB) model theory, the structural equation model(SEM)was used to analyze the effect of using new media to carry out AIDS health education among young students. Methods: Stratified random cluster sampling method was used to select 2 561 students from four colleges in Yantai to conduct a questionnaire survey, and to carry out various forms of new media AIDS health education for the intervention group. Data were analyzed with structural equation model. Results: After intervention，the awareness rate of AIDS were 85.9% and 94.0% respectively(χ2=46.67，P<0.01). The structural equation model fits well, and the fitted indices were: RMSEA=0.04, GFI=0.97, AGFI=0.96, IFI=0.97, CFI=0.98. The direct effects of new media interventions on prevention information and prevention motivation were 0.30 and 0.65 respectively. The influence of information and motivation on preventive behavior was mainly reflected through the indirect effect of behavioral skills. The overall effects of standardization were 0.09 and 0.29 respectively. The overall effect of behavioral skills on preventive behavior was 0.80, which was the main factor affecting HIV prevention behavior. Conclusion The use of new media to carry out AIDS health education among young students had a significant effect. Motivation promotion should be used as a new breakthrough point for future work, and information, motivation, and behavioral skills should be used to promote the prevention of AIDS prevention among young students.

Objective:To summarize the patient profiles and therapeutic efficacies of ABO-incompatible living-related kidney transplantations at 19 domestic transplant centers and provide rationales for clinical application of ABOi-KT.Methods:Clinical cases of ABO-incompatible/compatible kidney transplantation (ABOi-KT/ABOc-KT) from December 2006 to December 2009 were collected. Then, statistical analyses were conducted from the aspects of tissue matching, perioperative managements, complications and survival rates of renal allograft or recipients.Results:Clinical data of 342 ABOi-KT and 779 ABOc-KT indicated that (1) no inter-group differences existed in age, body mass index (BMI), donor-recipient relationship or waiting time of pre-operative dialysis; (2) ABO blood type: blood type O recipients had the longest waiting list and transplantations from blood type A to blood type O accounted for the largest proportion; (3) HLA matching: no statistical significance existed in mismatch rate or positive rate of PRA I/II between two types of surgery; (4) CD20 should be properly used on the basis of different phrases; (5) hemorrhage was a common complication during an early postoperative period and microthrombosis appeared later; (6) no difference existed in postoperative incidence of complications or survival rate of renal allograft and recipients at 1/3/5/10 years between ABOi-KT and ABOc-KT. The acute rejection rate and serum creatinine levels of ABOi-KT recipients were comparable to those of ABOc-KT recipients within 1 year.Conclusions:ABOi-KT is both safe and effective so that it may be applied at all transplant centers as needed.