Objective:To analyze the distribution and genomic characteristics of SARS-CoV-2 variants isolated from imported cases of COVID-19 in Sichuan Province from December 2022 to January 2023.Methods:This study selected 108 nasal and throat swab samples with fluorescence threshold (Ct)≤32 that were collected from imported COVID-19 cases in Sichuan Province from December 2022 to January 2023. Targeted amplification and Illumina NextSeq? 2000 system were used for whole-genome sequencing. Nextclade and Pangolin online platforms were used to determine the virus genotypes and analyze the mutation loci. A phylogenetic tree was constructed using maximum likelihood.Results:A total of 55 SARS-CoV-2 whole genome sequences with coverage of >95% were obtained, and all of the strains were Omicron variants. Compared with the sequence of reference strain Wuhan-Hu-1, the median number of nucleotide mutation sites of 21L, 22B, 22D, 22E, and 22F genotypes were 93, 75, 92, 78, and 92, and the median number of amino acid mutation sites were 68, 53, 68, 69, and 65, respectively. From December 2022 to January 2023, the predominant circulating SARS-CoV-2 variants from imported cases in Sichuan Province were BA.5.2 (10.91%, 6/55), XBB.1.1 (9.10%, 5/55), BF.7.14 (7.23%, 4/55), and BQ.1.1 (7.23%, 4/55).Conclusions:The distribution of SARS-CoV-2 variants can reflect the global epidemic trend to a certain extent. However, it is different from the distribution of local circulating variants in the Chinese mainland. The XXB variants with transmission advantages can be detected in large numbers of inbound travelers before becoming the predominant circulating strains in the Chinese mainland.

Objective:This study aimed to evaluate the changes of fractional amplitude of low frequency fluctuation (fALFF) in young depressed patients with suicide attempts after cognitive behavior therapy (CBT) combined with fluoxetine by using the resting-state functional magnetic resonance imaging (rs-fMRI).Method:From March 2017 to December 2019, 45 young depressed patients with suicide attempts were recruited from the Second People′s Hospital of Guizhou Province. The patients were randomly allocated into CBT combined with antidepressant treatment (combination group) and antidepressant treatment alone (antidepressant group). Individuals in combination group were treated with 8-time CBT combined with fluoxetine, patients in antidepressant group were treated with fluoxetine alone. Healthy volunteers matched with the age, gender and education level of the patients were recruited as the control group. The rs-fMRI data were collected before and after the treatment.The clinical symptoms and the suicidal ideation were evaluated by HAMD 24 scale and the Scale for Suicidal Ideation (SSI). The brain function in response to the treatment was analyzed by fALFF, change after the treatment. And the correlation analysis was explored between the brain function and the clinical symptoms. Result:At the baseline, compared with the control group, the fALFF values of combination group were significantly increased in the left posterior lobe ( t=4.312), right anterior cingulate cortex ( t=4.079), left inferior parietal lobe ( t=3.983), left superior temporal cortex ( t=3.983), left caudate nucleus ( t=4.109), left superior frontal cortex ( t=5.191) and left middle frontal cortex ( t=5.191), while significantly decreased in left angular gyrus ( t=-4.607). After 8 weeks of treatment, the depressive symptoms and the suicidal ideation in combination group were significantly improved compared to those in antidepressant group. Compared to antidepressant group, higher fALFF values were found in combination group in right middle frontal cortex ( t=3.456), right inferior frontal cortex ( t=3.456), left posterior cerebellar lobe ( t=5.120), and left anterior cerebellar lobe ( t=5.120); lower values were found in left medial frontal cortex ( t=-4.045), and left anterior cingulate cortex ( t=-4.045; P<0.05, AlphaSim correction). In combination group, the value of fALFF in left superior frontal cortex was negatively correlated with the score of HAMD 24( r=-0.600, P=0.002), and that of right anterior cingulate cortex was positively correlated with SSI score ( r=0.428, P=0.037); after treatment, the value of fALFF in right medial prefrontal lobe was positively correlated with the SSI improvement (ΔSSI) ( r=0.518, P=0.010). Conclusion:After 8 weeks of CBT combined with fluoxetine treatment, depressive symptoms may get more improved with less suicidal ideation than the fluoxetine treatment alone in young depressed patients who have suicide attempts before, and the local spontaneous brain activities change accordingly, especially in the frontal limbic system and cerebellum. It may suggest that CBT combined with fluoxetine may effectively regulate the spontaneous activities of these brain regions and possibly reshape the emotional processing as well as the cognitive circuit which contribute the symptoms improvement.

Objective:To evaluate the effect of intestinal carbapenem-resistant Enterobacteriaceae (CRE) active screening combined with enhanced intervention in the prevention and control of nosocomial infection in patients admitted to the hematological ward.Methods:Patients who were admitted to the Department of Hematology in a tertiary-care general hospital from March 1, 2017 to December 31, 2019 and underwent chemotherapy or immunosuppressive therapy comprised the intervention group. They were screened for intestinal CRE at least thrice. From December 1, 2016 to February 28, 2017, patients who underwent chemotherapy or immunosuppressive therapy without active intestinal CRE screening in the Department of Hematology formed the control group. Both the patient groups were monitored for CRE infection in real time. The χ2 test was used to compare the changes in the CRE infection rate and mortality in high-risk patients before and after the active screening. Results:During the intervention period, the CRE colonization rate of patients was 16.46% (66/401) ; in terms of disease distribution, the colonization rate of acute leukemia was the highest 23.03% (26/113) . Of the 66 colonized patients, 27 (40.9%) patients were identified as positive for CRE at the first screening, 15 (22.7%) were identified at the time of the second screening, and the remaining 24 (36.4%) were identified at the third or subsequent screening; Carbapenem-resistant Klebsiella pneumoniae (CRPK) strains were dominant among the pathogens, accounting for 54.55% (36/66) . During the active screening period, the CRE infection rate (2.49%) and mortality rate (50.00%) of high-risk patients were significantly lower than those of the controls (11.30% and 69.23%, respectively) . The pathogens of 10 CRE infection patients during the intervention period were exactly the same as the previous active screening pathogens, and the coincidence rate was 100%.Conclusion:The CRE colonization rate was the highest in patients with acute leukemia who were admitted in the hematology wards. CRPK is the main pathogen of CRE colonization, infection, and death. Increasing the frequency of screening can significantly raise the positive rate of screening, Active screening can effectively reduce the incidence and subsequent mortality of CRE in high-risk patients admitted in the hematological wards. High coincidence rate between CRE screening positive pathogens and subsequent CRE infection pathogens. Intestinal CRE screening can serve as an indicator of CRE bloodstream infection in patients with hematological diseases as well as provide information for antibiotics therapy.

Objective:This study aimed to evaluate the changes of fractional amplitude of low frequency fluctuation (fALFF) in young depressed patients with suicide attempts after cognitive behavior therapy (CBT) combined with fluoxetine by using the resting-state functional magnetic resonance imaging (rs-fMRI).Method:From March 2017 to December 2019, 45 young depressed patients with suicide attempts were recruited from the Second People′s Hospital of Guizhou Province. The patients were randomly allocated into CBT combined with antidepressant treatment (combination group) and antidepressant treatment alone (antidepressant group). Individuals in combination group were treated with 8-time CBT combined with fluoxetine, patients in antidepressant group were treated with fluoxetine alone. Healthy volunteers matched with the age, gender and education level of the patients were recruited as the control group. The rs-fMRI data were collected before and after the treatment.The clinical symptoms and the suicidal ideation were evaluated by HAMD 24 scale and the Scale for Suicidal Ideation (SSI). The brain function in response to the treatment was analyzed by fALFF, change after the treatment. And the correlation analysis was explored between the brain function and the clinical symptoms. Result:At the baseline, compared with the control group, the fALFF values of combination group were significantly increased in the left posterior lobe ( t=4.312), right anterior cingulate cortex ( t=4.079), left inferior parietal lobe ( t=3.983), left superior temporal cortex ( t=3.983), left caudate nucleus ( t=4.109), left superior frontal cortex ( t=5.191) and left middle frontal cortex ( t=5.191), while significantly decreased in left angular gyrus ( t=-4.607). After 8 weeks of treatment, the depressive symptoms and the suicidal ideation in combination group were significantly improved compared to those in antidepressant group. Compared to antidepressant group, higher fALFF values were found in combination group in right middle frontal cortex ( t=3.456), right inferior frontal cortex ( t=3.456), left posterior cerebellar lobe ( t=5.120), and left anterior cerebellar lobe ( t=5.120); lower values were found in left medial frontal cortex ( t=-4.045), and left anterior cingulate cortex ( t=-4.045; P<0.05, AlphaSim correction). In combination group, the value of fALFF in left superior frontal cortex was negatively correlated with the score of HAMD 24( r=-0.600, P=0.002), and that of right anterior cingulate cortex was positively correlated with SSI score ( r=0.428, P=0.037); after treatment, the value of fALFF in right medial prefrontal lobe was positively correlated with the SSI improvement (ΔSSI) ( r=0.518, P=0.010). Conclusion:After 8 weeks of CBT combined with fluoxetine treatment, depressive symptoms may get more improved with less suicidal ideation than the fluoxetine treatment alone in young depressed patients who have suicide attempts before, and the local spontaneous brain activities change accordingly, especially in the frontal limbic system and cerebellum. It may suggest that CBT combined with fluoxetine may effectively regulate the spontaneous activities of these brain regions and possibly reshape the emotional processing as well as the cognitive circuit which contribute the symptoms improvement.

Objective To investigate the clinical effects of preoperative eye position training on femtosecond laser-assisted laser in situ keratomileusis(FL-LASIK)? Methods One hundred and sixty-eight myopia patients(328 eyes)scheduled for selective FL-LASIK were randomly divided into the experiment group of 85 cases(166 eyes)and the control group of 83 cases(162 eyes)?The patients in the control group were given the routine preoperative education and the patients in the experiment group received the preoperative intervention of eye position besides routine preoperative education? The two groups were compared in terms of intraoperative changes of eye position,corneal topography and the postoperative visual acuity? Results The frequency of eye position adjustment and machine stopping in the experiment group was significantly smaller than that of the control group(P < 0?001)? But no significant differences were found between the two groups in corneal topography and the postoperative visual acuity(P > 0?05)? Conclusion Preoperative training of eye position may maintain ideal eye position and reduce the frequency of downtime due to eye position adjustment during FL-LASIK,which may ensure the successful completion of FL-LASIK?

Protocol design and implementation are important steps for clinical trial . This article discussed key issues on effect of live birth rate by polycystic ovary syndrome ( PCOS ) treatment with acupuncture combined with clomiphene citrate in the randomized controlled trial ( RCT ) process . For example , the trial designs ( i . e . , trial design characteristics , selection of featured therapeutic effect index ) , clinical trial implementation ( i . e . , or-ganization construction , quality control , and etc . ) . This summarization provided references for the design and implementation of clinical trial plan .

Four genotypic mice were constructed, by ovary transplantation, with body leptin receptor (LR) + and ovary LR - in Group A, body LR + and ovary LR + in Group B, body LR - and ovary LR + in Group C, as well as body LR - and ovary LR - in Group D respectively. Mice in C and D groups showed significant differences in glucose and fat metabolism as well as ovarian function as compared with A and B groups. Leptin signal transducting system exists in the ovary. Ovarian failure was induced by hyperlipidemia and lowered gonadotropin levels, but not by defective leptin signal within ovaries in the db/db mice.

OBJECTIVE: To investigate the effect of ginseng total saponins (GTS) on expression of nerve growth factor in rat with polycystic ovaries. METHODS: A total of thirty rats were randomly divided into normal control group, untreated group and GTS group. Polycystic ovaries were induced by a single intramuscular injection of 4 mg estradiol valerate (EV) in rats of the untreated group and GTS group. The rats in the GTS group were administered 50 mg/kg GTS every other day by intraperitoneal injection for 30 days after the EV injection. The expressions of NGF in the ovaries, pituitary and hippocampus were observed by method of immunohistochemistry. RESULTS: Comparison with the normal control group revealed that the expressions of NGF in ovaries, pituitary and hippocampus of rats in the untreated group were increased (P<0.05). The ovarian morphology of the GTS group was almost as normal as that of the normal control group. As compared with the untreated group, the expression of NGF in ovaries of the rats in GTS group was obviously decreased (P<0.05), while the expression of NGF in pituitary and hippocampus showed on difference. CONCLUSION: GTS can decrease the expression of NGF in ovary tissue in rats with polycystic ovaries induced by EV, which may be its mechanism in lessening the polycystic ovary.

Objective To observe the features of the manifestations of fundus fluorescein angiography (FFA) in multiple sclerosis (MS) and their value in clinical diagnosis. Methods The clinical data of 42 patients (84 eyes) with MS diagnosed by magnetic resonance imaging (MRI) and examination of cerebrospinal fluid (CSF) were retrospectively analyzed. The clinical data included visual acuity, ocular fundus examined by direct ophthalmoscope after mydriasis, FFA, visual field, CSF,visual evoked potential (VEP) and MRI examination. Results In 42 patients (84 eyes),the positive detectable rate of examination of direct ophthalmoscope, CSF, visual field, VEP, and MRI was 36.9%, 21.4%, (71.4%, and) 83.3% respectively. Abnormal results of FFA were found in 44 eyes (52.38%), including papillitis in 4 eyes (4.76%)at the early stage with extended physiological scotoma and central scotoma; neuroretinitis in 7 eyes (8.33%)at the medium stage with central or para- central scotoma; optic atrophy in 33 eyes(39.29%) at the late stage with centripetal constriction and even tubular visual field. Conclusion The main angiographic features of MS are papillitis, neuroretinitis and optic atrophy. The manifestations of FFA combined with the results of examination of CSF,visual field, VEP and MRI is helpful for comprehensive and exact diagnosis of MS.