To evaluate the therapeutic effect of baicalein topical preparation on atopic dermatitis, we first constructed two atopic dermatitis-like mouse models induced by calcipotriol and 1-fluoro-2,4-dinitrobenzene to assess their therapeutic effect with skin tissue staining and other experiments. It was found that topical preparation of baicalein could alleviate epidermal thickening of diseased skin tissues, repair damaged skin barrier proteins, and inhibit T helper 2 cells (Th2) infiltration and mast cell infiltration and activation in lesional sites. Cyberpharmacology was utilized to analyze whether baicalein could treat atopic dermatitis by interfering with multiple pathogenesis-associated pathways. Results indicated that baicalein reduced the mRNA levels of inflammatory factors and inhibited the phosphorylation of NF-κB p65 and STAT1 proteins in keratinocyte cells. Together, the topical preparation of baicalein may be effective in alleviating atopic dermatitis-like symptoms in mice by down-regulating the phosphorylation level of NF-κB in keratinocytes, thereby decreasing the expression of inflammatory factors in keratinocytes, which provides an idea and a theoretical basis for the topical preparation of baicalein for the treatment of inflammatory skin diseases such as atopic dermatitis.

Objective To investigate the influencing factors and imaging characteristics of medial meniscus posterior horn root tear with displacement caused by military training.Methods The clinical data and magnetic resonance imaging(MRI)characteristics of 102 patients with root tears of the posterior horn of the medial meniscus confirmed by arthroscopy in Hebei Provincial Armed Police Corps Hospital from July 2020 to June 2022 were retrospectively analyzed.There were 84 males and 18 females with a mean age of(33.06±5.72)years(21-45 years).According to the displacement after tear,the patients were divided into displacement group(n=35)and non-displacement group(n=67).The clinical data and imaging characteristics were compared between groups.Multivariate regression analysis was used to analyze the risk factors of medial meniscus posterior horn root tear combined with displacement caused by military training.The diagnostic efficacy of MRI for the root tear of medial meniscus posterior horn with displacement was evaluated.Results The absolute amount of meniscus prolapse,the relative rate of meniscus prolapse and the proportion of patients with grade Ⅲ/Ⅳ cartilage degeneration of medial tibial plateau in the displacement group were significantly higher than those in the non-displacement group(P<0.05).Multivariate regression analysis showed that the absolute amount of meniscus prolapse(OR=2.971,95%CI:1.142-8.733),the relative rate of meniscus prolapse(OR=3.177,95%CI:1.227-9.261),and grade Ⅲ/Ⅳ cartilage degeneration of the medial tibial plateau(OR=2.930,95%CI:1.125-7.632)were related to medial meniscus posterior horn root tear with displacement caused by military training(P<0.05).Receiver operating characteristic curve analysis showed that the sensitivity,specificity and area of the curve(AUC)of MRI in the diagnosis of medial meniscus posterior horn root tear with displacement were 85.71%(95%CI:68.96%-94.62%),91.04%(95%CI:80.88%-96.31%)and 0.884(95%CI:0.805-0.939),respectively.Conclusion Medial meniscus posterior horn root tear with displacement caused by military training is related to MRI imaging parameters such as absolute amount of meniscus prolapse,relative of meniscus prolapse rate,and cartilage degeneration of medial tibial plateau.MRI has a high diagnostic efficiency for medial meniscus posterior horn root tear with displacement.

The accumulation of fatigue during military operations may lead to decreased operational efficiency and non-combat attrition,which can impact combat effectiveness.On-site monitoring and evaluation of fatigue during military operations,as an important means to keep track of military operations and bring about quick changes in training,underlie the combat effectiveness of military personnel.Focusing on the on-site monitoring and evaluation methods of fatigue during military operations,this paper reviews the determinants of such fatigue as well as on-site monitoring and comprehensive evaluation methods so as to provide reference for accurate and efficient evaluation of fatigue during military operations and for early warning of such fatigue.

Cognitive impairment belongs to the categories of"foolish disease"and"delusional syndrome"in traditional Chinese medicine,the incidence rate is increasing year by year,and regulating amino acid metabolism may be an important mechanism for the treatment of cognitive dysfunction from the spleen.Amino acids are the essence dispersed by the spleen,which can be used as potential biomarkers.Amino acid neurotransmitters are scattered in the brain,convey the meaning of the spleen,and participate in neuronal communication and cognitive activities.The treatment method is mainly to nourish the spleen and invigorate qi,warm yang and strengthen the spleen,nourish yin and nourish the spleen,and also dissolve phlegm,dispel stasis and detoxify,so as to regulate amino acid metabolism,restore the main function of the spleen,and improve cognitive dysfunction.Under the guidance of the theory of"spleen governs dispersing essence and storing meaning",the regulation of amino acid metabolism provides a new theoretical basis and research strategy for the treatment of cognitive dysfunction diseases in traditional Chinese medicine.

Cognitive impairment belongs to the categories of"foolish disease"and"delusional syndrome"in traditional Chinese medicine,the incidence rate is increasing year by year,and regulating amino acid metabolism may be an important mechanism for the treatment of cognitive dysfunction from the spleen.Amino acids are the essence dispersed by the spleen,which can be used as potential biomarkers.Amino acid neurotransmitters are scattered in the brain,convey the meaning of the spleen,and participate in neuronal communication and cognitive activities.The treatment method is mainly to nourish the spleen and invigorate qi,warm yang and strengthen the spleen,nourish yin and nourish the spleen,and also dissolve phlegm,dispel stasis and detoxify,so as to regulate amino acid metabolism,restore the main function of the spleen,and improve cognitive dysfunction.Under the guidance of the theory of"spleen governs dispersing essence and storing meaning",the regulation of amino acid metabolism provides a new theoretical basis and research strategy for the treatment of cognitive dysfunction diseases in traditional Chinese medicine.

Objective: To investigate the neural connections between Shenmen (HT7)-heart and the brain by observing the tracing viruses co-labeled brain nuclear groups after injection of the pseudorabies viruses (PRV), the reverse transsynaptic virus tracer carrying different fluorescent protein genes, into the myocardium and Shenmen (HT7) point, respectively.Methods: Pseudorabies virus 531 (PRV531) carrying the green fluorescent protein gene and pseudorabies virus 724 (PRV724) carrying the red fluorescent protein gene were injected into the left ventricular wall and Shenmen (HT7) point area of the left forelimb of six C57BL/6 mice, respectively. After 120 h, whole brain tissue was extracted under 4％ paraformaldehyde perfusion to prepare brain sections. Neuronal co-labeling with the tracing viruses was observed under fluorescence microscopy. Results: Co-labeled signals from the mouse ventricular wall and Shenmen (HT7) point region were found at all levels of the mouse central nervous areas, such as the cerebral cortex, hypothalamus, midbrain, pons, and medulla oblongata. The number of co-labeled neurons was higher in the primary motor area, the hypothalamic paraventricular nucleus, the subceruleus nucleus, and the paramedian reticular nucleus. Conclusion: There is a neural connection between Shenmen (HT7), the heart, and the brain, which may be most closely related to the autonomic nervous system.

Objective : To investigate the effects of p100Rband p80Rbtruncated forms of retinoblastoma protein(Rb) on HEK 293T cell cycle and apoptosis. Methods : The eukaryotic expression plasmid pcDNA3. 1-p100Rb-FLAG and pcDNA3. 1-p80Rb-FLAG were constructed,plasmid was transfected into U2OS cells and immunofluorescence was performed to define the localization of p100Rband p80Rbin cells. Plasmid was also transfected into HEK 293T cells,and the total protein was extracted for Western blotting to detect the protein expression level. After overexpression of Rb,p100Rband p80Rbin HEK 293T cells,cell cycle and apoptosis were measured by flow cytometry. Results: The eukaryotic expression plasmid pcDNA3. 1-p100Rb-FLAG and pcDNA3. 1-p80Rb-FLAG were constructed successfully. p100Rbwas mainly located in the nucleus while p80Rbwas mainly located in the cytoplasm,in U2OS cells. Rb,p100Rband p80Rbcould be overexpressed in HEK 293T cells. Following overexpression of Rb,p100Rbin HEK 293T cells,the percentage of G1phase of cell cycle significantly increased compared with the control group,and p100Rband p80Rbinhibited apoptosis compared with control group(P<0. 05). Conclusion Overexpression of p100rb can inhibit the apoptosis of HEK 293T cells and block the cell cycle. Overexpression of p80rb can inhibit the apoptosis of HEK 293T cells,but has no effect on cell cycle.

Objective : To investigate the interaction between intracellular chloride ion protein 1(CLIC1) and activated protein kinase C receptor 1 ( RACK1) . Methods : Plasmids pcDNA3. 1⁃RACK1⁃HA and/or pcDNA3. 1 ⁃CLIC1⁃FLAG were transfected into HEK 293T cells, and pcDNA3. 1⁃RACK1⁃HA and/or pcDNA3. 1⁃CLIC1⁃FLAG were transfected into COS7. GST⁃pulldown and immunoprecipitation assays were performed to determine the interaction between CLIC1 and RACK1 in vivo and in vitro. The co⁃localization of CLIC1 and RACK1 was observed by indirect immunofluorescence assay. Results : Western blot confirmed that CLIC1 and RACK1 could be highly expressed in HEK 293T cells. GST⁃pulldown showed that RACK1 bound CLIC1 in vitro, and immunoprecipitation showed that CLIC1 and RACK1 interacted in vivo. Furthermore, indirect immunofluorescence assay showed CLIC1 co⁃localized with RACK1 . Conclusion Human CLIC1 protein interacts with RACK1 in vitro and in vivo.

Objective : To investigate the effects of fukutin ( FKTN) which is a member of ribitol⁃5⁃phosphate transferases on HeLa cell cycle, apoptosis, migration and the secretion of α⁃dystroglycan(α⁃DG) . Methods : The plasmid pcDNA3. 1⁃α⁃DAG⁃HA was constructed. HeLa cells were transfected with plasmid pcDNA3. 1⁃FKTN ⁃ 3xFlag, then the total protein was extracted for Western blot to determine the expression level of FKTN. Cell cycle and apoptosis were measured by flow cytometry after the overexpression of FKTN. Following the overexpression of FKTN, wound⁃healing assay was performed to detect the cell migration rate, as the same time wheat germ agglutinin⁃agarose (WGA⁃agarose) was used to enrich α⁃DG in the cell cultures, then α⁃DG was detected by Western blot. Results : The eukaryotic expression plasmid pcDNA3. 1⁃α⁃DAG⁃HA was constructed successfully. FKTN could be overexpressed in HeLa cells. After the overexpression of FKTN,the percentage of S phase of cell cycle in the experimental group decreased (P < 0. 001) and apoptosis rate unchanged (P > 0. 05) when compared with the control group. There was no change in the cell migration rate of experimental group (P > 0. 05), but after the overexpression of FKTN, secretion of α⁃DG decreased when compared with control group. Conclusion Overexpressing FKTN arrests cell cycle and inhibits the secretion of α⁃DG in HeLa cells. Apoptosis and cell migration of HeLa cells are not affected by the overexpression of FKTN.

Objective:To explore the mechanism of Dahuangfuzi decoction on intestinal motility disorder by observing its effect on serum motilin, Cajal interstitial cells and motilin receptor in rats with severe acute pancreatitis (SAP).Methods:Eighteen clean male Sprague-Dawley rats were randomly divided into the control group, SAP group and Dahuangfuzi group ( n=6 each group). The SAP rat model was prepared by retrogradely injected 4% sodium taurocholate into cholangiopancreatic duct. The rats in the SAP group were given 2 mL normal saline (37℃) enema at 12 and 24 h after operation. The rats in the Dahuangfuzi group was given 2 mL Dahuangfuzi decoction (37℃) enema at 12 and 24 h respectively. For the control group, the pancreas was exposed in the same way and then the abdomen was closed. Forty-eight h after operation, the abdominal aorta blood samples were taken for determination of serum endotoxin and amylase, and for detection of serum motilin by enzyme-linked immunosorbent assay (ELISA); the pathological changes of pancreas and ileum were observed by hematoxylin-eosin (HE) staining. The expression of c-kit and motilin receptor protein in ICC in ileum tissue was detected by immunohistochemistry. Results:Compared with the control group, the levels of serum endotoxin and amylase in the SAP group were significantly higher [(504.98±88.81) pg/mL vs. (17.76±5.01) pg/mL; (532.28±66.53) vs. (69.45±3.61) U/L, P<0.05], while the levels of serum motilin were significantly lower [(195.4±6.7) ng/L vs. (301±8.10) ng/L, P<0.05], and the scores of c-kit and motilin receptor protein were decreased ( P<0.05); compared with the SAP group, the levels of serum endotoxin and amylase in the Dahuangfuzi group were significantly reduced [(189.9±38.23) pg/mL vs. (504.98±88.81) pg/mL; (294.23±25.66) vs. (532.28±66.53) U/L, P<0.05], while the levels of serum motilin were significantly increased [(264.2±8.3) ng/L vs. (195.4±6.7) ng/L, P<0.05], and the scores of c-kit and motilin receptor protein were increased ( P<0.05). Conclusions:Dahuangfuzi decoction can improve the intestinal motility of SAP rats by promoting the secretion of motilin, increasing the activity of ICC cells and the expression of motilin receptor.