Objective To investigate the clinical effectiveness of treatment combining Honghe lotion and clotrimazole in patients with colpitis mycotica in pregnancy. Methods 200 patients with colpitis mycotica in pregnancy in our hospital from January, to September, 2017 were randomized into observation and control group with 100 patients in each. Patients in control group were treated with clotrimazole while patients in observation group were treated with additional Honghe lotion. Effectiveness at the end of course of treatment, the time of symptom disappearance, satisfactory rate of treatment, adverse effect and recurrence during 6-month follow-up in two groups were compared. Results Total effective rate at the end of course of treatment in observation group was significantly higher than that in control group and the rate of recurrence after 6-month follow-up was significantly lower than that in control group(P＜0. 05). Time of symptom disappearance in observation group was significantly shorter and the satisfactory rate of treatment in observation group was significantly higher than those in control group respectively (P＜0. 05). There was no adverse effects observed in two groups during treatment. Conclusion Treatment combining Honghe lotion and clotrimazole in patients with colpitis mycotica in pregnancy was effective with low rate of recurrence and good safety. It was worth clinical promotion.