"Weibing" is a fundamental concept in traditional Chinese medicine (TCM), representing a transitional state characterized by diminished self-regulatory abilities without overt physiological or social dysfunction. This perspective delves into the biological foundations and quantifiable markers of Weibing, aiming to establish a research framework for early disease intervention. Here, we propose the "Health Quadrant Classification" system, which divides the state of human body into health, sub-health, disease-susceptible state, and disease. We suggest the disease-susceptible stage emerges as a pivotal point for TCM interventions. To understand the intrinsic dynamics of this state, we propose laboratory and clinical studies utilizing time-series experiments and stress-induced disease susceptibility models. At the molecular level, bio-omics technologies and bioinformatics approaches are highlighted for uncovering intricate changes during disease progression. Furthermore, we discuss the application of mathematical models and artificial intelligence in developing early warning systems to anticipate and avert the transition from health to disease. This approach resonates with TCM's preventive philosophy, emphasizing proactive health maintenance and disease prevention. Ultimately, our perspective underscores the significance of integrating modern scientific methodologies with TCM principles to propel Weibing research and early intervention strategies forward.

Mitochondrial disease is one of the major types of inherited metabolic disease that can affect all age groups,particularly in children where it has a high mortality and disability rate.With the development of biochemical,molecular,and cellular biology techniques,the laboratory diagnosis of mitochondrial disease has undergone rapid development.The diagnostic pathways and strategies have gradually transitioned from highly invasive laboratory tests to mainly non-invasive screenings.However,the challenge remains that the positive diagnostic rate of single testing strategies is insufficient,and the proportion of missed and pending investiga-tions remains high.Consequently,new mitochondrial disease laboratory diagnostic techniques continue to e-merge and are used to aid in disease diagnosis.This review attempts to summarize the current progress in mi-tochondrial disease laboratory diagnostics at three levels:genetics,enzyme biochemistry,and metabolic biolo-gy,providing references for the selection of laboratory diagnostic strategies in specific scenarios,as well as suggestions for the development of future detection technologies.

Objective:To evaluate the effectiveness of contrast-enhanced ultrasound in the differential diagnosis of atypical adenomyosis and fibroids using a decision tree model.Methods:The data of cases with difficulty in differentiating atypical adenomyosis from fibroids on conventional ultrasound examination at Shengjing Hospital of China Medical University from April 2021 to April 2022 were selected and analyzed. Ninety-five patients with contrast-enhanced ultrasound examination were finally selected, including 64 patients in the pathologically confirmed adenomyosis group and 31 patients in the fibroids group. The data from the qualitative analysis and the quantitative analysis of the time-intensity curve (TIC) curve were collected separately, including the temporal variability of contrast entry into the lesion, i.e.the difference between the time when the contrast agent started to enter the lesion and the time when the contrast agent finally filled the lesion completely. Indicators were first screened for inclusion in the decision tree model by univariate and multifactorial analyses, and decision tree models based on qualitative analysis indicators, and qualitative and TIC-based analyses were developed to further assess the diagnostic efficacy of both models.Results:Through the univariate analysis, it showed that the qualitative analysis indicators of lesion onset enhancement pattern, enhancement intensity, intra-lesion contrast distribution, and post-contrast lesion border were of statistical significance (all P<0.05) between the two groups. The differences in contrast arrive time (AT), contrast time to peak (TTP), |ΔAT|, and |ΔTTP| in the TIC curve analysis indexes were statistically significant between the two groups (all P<0.05). The difference in lesion temporal phase variability was statistically significant between the two groups ( P<0.05). After further screening by multifactorial analysis, the accuracy and misdiagnosis rates were 87.40% and (17.90±3.90)% in the qualitative analysis-based decision tree model respectively, and 90.50% and (21.10±4.20) % in the qualitative and TIC curve-based analysis decision tree model respectively. The ROC curves were plotted according to the two groups of models, and the areas under the curves were 0.915 and 0.931 respectively. Conclusions:A decision tree model based on ultrasonographic image analysis has diagnostic value for the differential diagnosis of atypical adenomyosis and uterine fibroids.

OBJECTIVE: To discuss the endovascular interventional treatment of pseudoaneurysm of extracranial segment of internal carotid artery. METHOD: The clinical data of 5 cases with traumatic pseudoaneurysm of extracranial segment of internal carotid artery were retrospectively studied. RESULT: All the patients were cured via endovascular interventional treatment, and performed with implanting covered stent into the parent artery. The patients recovered well with patency of the parent artery. CONCLUSION Endovascular interventional treatment with covered stent implantation was safe and effective in treatment of pseudoaneurysm of extracranial segment of internal carotid artery.
Adolescent ; Adult ; Aneurysm, False ; therapy ; Blood Vessel Prosthesis Implantation ; Carotid Artery, Internal ; Child ; Female ; Humans ; Male ; Retrospective Studies ; Stents ; Treatment Outcome ; Young Adult

Objective To study the feasibility and clinical significance of internal tourniquet (temporary arterial lumen blood flow occlusion by balloon catheter) for controlling the hemorrhage from traumatic neck and adjacent trunk arteries by temporarily occluding the arterial lumen blood flow.Methods The study involved 35 patients with traumatic neck and near trunk arteries who were firstly managed by internal tourniquet during operation to temporarily occlude the proximal aorta blood flow from May 1987 to February 2009.Each blocking time ranged from 30 to 70 minutes and the blocking was performed at an interval of 15 to 20 minutes.Then,surgical therapy was taken.Results After temporary proximal aorta blood flow occlusion with internal tourniquet,the operation presented few bleeding,with a clean operating field and clear anatomic structures.The total intraoperative blood loss was 100-400 ml.All patients were healed without ischemia of brains and limbs or relapse during the 3-14 years of followup.Conclusion Internal tourniquet,which can effectively reduce intraoperative blood loss and improve operation safety by temporarily occluding the proximal aorta blood flow,is an auxiliary approach for treating hemorrhage from traumatic neck and adjacent trunk arteries.

Objective To study the clinical value of CT function supplied by DSA system. Methods 278 patients were scanned by Innova CT ,which was concomitant with Innova 3100 DSA system, to check fresh blood .In the PTC operation, Innova CT was used to direct the site,degree,deepness of needle. Results Innova CT found intracranial hemorrhage in 4 cases . The fresh blood were checked by helical -CT,too. Innova CT comparing with B type ultrasonograph ,the orientation of needle were more convenient, exact, faster. Conclusion Innova CT could perform CT scan in interventional room to save time for emergence patients. It is a great improvement that gets CT images by DSA system. There is better consistency between Helix CT and Innova CT.

Objective To evaluate the therapeutic efficacy of the method of standard large trauma craniotomy in combination with therapeutic moderate hypothermia.Method Patients with acute severe head injuries (GCS≤8)were assigned into two groups:Group I,30 cases(average GCS≤ 5.38) received standard large trauma craniotomy and moderate hypothermia for 5-7 days;Group Ⅱ,30 cases(average GCS≤5.46)were treated with traditional treatment as control.Mortality,GCS,GOS and intracranial pressure (ICP) were compared between these two groups.Result Both the GCS and GOS were higher in the group Ⅰ than group Ⅱ;while the mortality and ICP were obviously lower in the group Ⅰ than that of the control group.Conclusions Early standard large trauma craniotomy plus adequate decompression followed by postoperative therapeutic moderate hypothermia would be helpful in reducing the mortality moderate and morbidity of patients with severe head injuries,their level of living accommodation were improved as well.

Objective To explore the in vitro culture conditions for neural stem cells to differentiate into neuron-like cells.Methods Neural stem cells were induced to differentiate by a medium containing bFGF and heparin through adhesive culture.Immunohistochemistry was used to identify the expressions of NSE and GFAP after differentiation.Results Positive cells for NSE were 47.3%?1.7% on the 7th day after induction.The number of NSE positive cells in the induction group was 3 times as many as that in the control group(P

Objective To explore a way that induce human bone marrow mesenchymal stem cells to differentiate into neuron-like cells in vitro.Methods Bone marrow mesenchymal stem cells were cultivated in a medium containing serum by using density gradient centrifugation method and whole marrow method and were induced to differentiation by ?mercaptoethanol.Immunohistochemistry were used to identify the type of cells.Results The number of cells was((1.73)?(0.44))?10~(7)/ml by using whole marrow method and ((7.65)?(0.52))?10~(7)/ml by using density gradient centrifugation method at the 10th day cultivation respectively.There was statistically significant difference(P