Objective To explore the correlation between the location of adult peptic ulcers and Helicobacter pylori(Hp)infection and antibody typing,as well as its clinical significance.Methods A retrospective analysis was conducted on the serum Hp antibody typing results of adult patients 850 cases with peptic ulcers at Xuzhou Central Hospital from 2017 to 2024.According to the location of ulcers:duodenal ulcer group(DU group,n=429),gastric ulcer group(GU group,n=291)and ulcerative colitis group(UC group,n=130)were divided into three groups,and the relationship between ulcer location and Hp immunophenotyping was analyzed.The test results of 232 adult health examinees were used as the control group.Results The overall Hp infection rate in peptic ulcers patients was 79.76%(678/850),mainly with high virulence type I,with an rate of 62.71%(533/850),and type II was 17.06%(145/850).The Hp infection rates in the DU group,GU group,UC group and control groups were 86.48%(371/429),79.04%(230/291),59.23%(77/130)and 56.47%(131/232)respectively.The infection rates of type I were 72.96%,57.73%,40.00%and 47.41%,respectively.The infection rates of type II were 13.52%,21.31%,19.23%and 9.05%,respectively.The total infection rate,type I infection rate and type II infection rate of Hp were compared among the DU group,GU group,UC group and control group,and the differences were all statistically significant(χ2=91.838,67.169,17.647,all P<0.05).Compared with the UC group,the overall infection rate and type I infection rate of Hp in the DU group were the highest,followed by the GU group,and the differences were statistically significant(χ2=11.324～47.800,all P<0.05).However,the overall infection rate and the infection rate of type I were compared between the UC group and the control group,and the differences were not statistically significant(χ2=0.261,1.852,all P>0.05).The infection rate of type Ⅱ in GU group and UC group was significantly higher than that in controls groups,and the differences were statistically significant(χ2=14.518,7.782,all P<0.05).In addition,the positive rate of dual antibodies against CagA+VacA in the four groups was significantly higher than that of single antibody against CagA,VacA,and the differences were statistically significant(χ2=7.538～407.569,all P<0.05).Conclusion Adult patients with peptic ulcers are mainly infected with Hp type I strains,and the infection rate of type I strains is the highest in the DU group,followed by GU,while the infection rate of type I strains in UC is the lowest.UC is closely related to type II strain infection,and the infection rate is similar to that of GU patients.

Objective To explore the correlation between the location of adult peptic ulcers and Helicobacter pylori(Hp)infection and antibody typing,as well as its clinical significance.Methods A retrospective analysis was conducted on the serum Hp antibody typing results of adult patients 850 cases with peptic ulcers at Xuzhou Central Hospital from 2017 to 2024.According to the location of ulcers:duodenal ulcer group(DU group,n=429),gastric ulcer group(GU group,n=291)and ulcerative colitis group(UC group,n=130)were divided into three groups,and the relationship between ulcer location and Hp immunophenotyping was analyzed.The test results of 232 adult health examinees were used as the control group.Results The overall Hp infection rate in peptic ulcers patients was 79.76%(678/850),mainly with high virulence type I,with an rate of 62.71%(533/850),and type II was 17.06%(145/850).The Hp infection rates in the DU group,GU group,UC group and control groups were 86.48%(371/429),79.04%(230/291),59.23%(77/130)and 56.47%(131/232)respectively.The infection rates of type I were 72.96%,57.73%,40.00%and 47.41%,respectively.The infection rates of type II were 13.52%,21.31%,19.23%and 9.05%,respectively.The total infection rate,type I infection rate and type II infection rate of Hp were compared among the DU group,GU group,UC group and control group,and the differences were all statistically significant(χ2=91.838,67.169,17.647,all P<0.05).Compared with the UC group,the overall infection rate and type I infection rate of Hp in the DU group were the highest,followed by the GU group,and the differences were statistically significant(χ2=11.324～47.800,all P<0.05).However,the overall infection rate and the infection rate of type I were compared between the UC group and the control group,and the differences were not statistically significant(χ2=0.261,1.852,all P>0.05).The infection rate of type Ⅱ in GU group and UC group was significantly higher than that in controls groups,and the differences were statistically significant(χ2=14.518,7.782,all P<0.05).In addition,the positive rate of dual antibodies against CagA+VacA in the four groups was significantly higher than that of single antibody against CagA,VacA,and the differences were statistically significant(χ2=7.538～407.569,all P<0.05).Conclusion Adult patients with peptic ulcers are mainly infected with Hp type I strains,and the infection rate of type I strains is the highest in the DU group,followed by GU,while the infection rate of type I strains in UC is the lowest.UC is closely related to type II strain infection,and the infection rate is similar to that of GU patients.

Objective: To investigate the situation of social support (SS), and explore its relationship with antenatal depression (AD) among Zhejiang primiparas in their third trimesters. Methods: From March to August 2016, a cross-sectional study was conducted and the questionnaire was used at the outpatient consulting room of one maternity hospital in Hangzhou. Inclusion criteria includes the primiparas over 18 years old, gestation from 30 to 36 weeks, been able to understand and complete the questionnaires independently, no family history and history of mental disorders and no use of psychotropic drugs, without serious illness and so on. Exclusion criteria was that the primiparas were unable to complete all the contents of the questionnaire and of poor compliance. 1 150 questionnaires were actually given out, and 1 075 questionnaires were valid, so the valid rate was 93.48%. AD was evaluated by the Edinburgh Postnatal Depression Scale (EPDS) and SS was evaluated by the Perceived Social Support Scale (PSSS). SS between the non-AD group and the AD group was compared. The correlation between SS and AD was analyzed. Binary logistic regression model was used to assess the relationship between SS and AD. The level of SS was divided by average scores, groups lower than the average score was defined as the low-score group, groups higher than the average score was defined as the high-score group. Results: The prevalence of AD (score≥9) was 27.3% (293/1 075) among Zhejiang primiparas in their third trimesters. The scores of family support, friend support and other support, and the total score of SS among the non-AD group were 24.80±2.83, 23.40±3.00, 21.91±3.54 and 70.11±7.92, respectively, which were higher than those in the AD group (22.71±3.88, 21.45±3.59, 19.95±3.97, 64.10±10.01), (t values were 8.43, 8.29, 7.83 and 9.25, respectively, P<0.001 for all). The scores of family support, friend support and other support, and the total score of SS were negatively correlated with AD (rs values were-0.26,-0.25,-0.22 and-0.28, respectively, P<0.001 for all). Compared with low-score group, the scores of family support, friend support and other support, and the total score of SS among the high-score group had a lower risk of antenata depression among primiparas in their third trimesters, OR(95%CI) values were 0.56 (0.41-0.77), 0.66(0.47-0.92), 0.57(0.41-0.79) and 0.36(0.27-0.48), respectively. Conclusion The prevalence of AD among Zhejiang primiparas was relatively high, and AD was negatively associated with SS level. We suggest adding SS in community pregnancy health management service in the future.

Objective:To comparison of three different beta blockers on murine hemangioma (EOMA cells) cells in vitro and in vivo effects.Preliminary study on the therapeutic effect of propranolol on vascular tumor in mice and possible mechanisms , provide a reference for beta blockers in the treatment of infantile hemangioma .Methods: Comparative study on the effects of three kinds of different β-receptor blockers---metoprolol, propranolol and butoxamine , on the proliferation and apoptosis of Mouse Hemangioendothelioma Endothelial cell (EOMA cells) was conducted in vitro.EOMA cells were cultured in vitro,randomly divided into different groups,propranolol and timolol were added into the medium respectively ,after 24 h intervention.MTT assay and acridine orange staining assay were conducted respectively to detect cell viability and apoptosis level .EOMA cells were transplanted into nude mice in vivo.Tumor volume growth to 100 mm3 ,animals were randomly divided into 4 groups respectively ,the control group ,metoprolol group,Bhutto Samin group and propranolol group ,drug group according to 2 mg/( kg? d) oral gavage ,control group were given an equal volume of saline ( NS ) , every two days measurement tumor volume size .Serum levels of tumor necrosis factor alpha ( TNF-α) and vascular endothelial growth factor ( VEGF ) were detected by enzyme linked immunosorbent assay ( ELISA ) in the end of the experiment.Results:For propranolol,after 24 h treatment,significant differences of cell viability and apoptosis were noted (P<0.05) at the concentration of 50 μmol/L,while continuing to increase to 800 μmol/L,the cell survival rate decreased sharply to close to 10%. Acridine orange staining at the 50 μmol/L group after 24 h revealed many apoptotic cells .For metoprolol and butoxa mine ,significant differences of cell viability and apoptosis were noted ( P<0.05 ) at the concentration of 100 μmol/L,while continuing to increase to 800μmol/L,the cell survival rate decreased sharply to close to 20%.It was significantly higher than propranolol group at the same concentration ( P<0.05 ) .It showed a similar trend in acridine orange staining .In vivo experiments showed that the end of the experiment of metoprolol , butoxamine group and propranolol drugs in mice tumor volume , respectively ( 1 642.8 ±89.3 ) , ( 1 529.3 ± 119.1) and (752.7±46.5)mm3,significantly lower than the control group of mice tumor volume of (2 023.3±123.0) mm3(P<0.001).Metoprolol,butoxamine mice and propranolol drugs group ,serum VEGF levels for (606.5±105.8 ) pg/ml,(534.3±243.2 ) pg/ml and (420.1±123.7) pg/ml, significantly lower than the PBS control group [(825.8±145.7) pg/ml,(P<0.05)],the TNF alpha result was followed by(301.3±62.3) pg/ml,(305.1±53.8) pg/ml and (288.8±59.5) pg/ml,significantly lower than the normal control group [(444±100.4) pg/ml,P<0.05].Conclusion:Three kinds of beta-blockers can effectively inhibit EOMA cells proliferation and induce apoptosis in vitro, the role of propranolol more significantly than butoxamine and metoprolol .Three kinds of beta blockers restrain the growth of the hemangioma in vivo ,in which the inhibitory effect of propranolol is stronger than the metoprolol and butoxa mine.Three kinds of beta blockers can lower the levels of VEGF and TNF-αin vivo.Indicating that propranolol on vascular tumor in mice may be one of the mechanisms of β1 and β2 receptor synergy effect and its mechanism in the treatment of hemangioma may be associated with VEGF and TNF-α.