Objective T o validate the performance of the whole blood C-reactive protein(CRP)detection system of BC-7500CS automated hematology analyzer.Methods The whole blood CRP detection system of BC-7500CS automated hematology ana-lyzer was verified based on relevant industrial standards in terms of blank determination,carry-over contamination rate,precision,linear range,comparability of different detection systems,correctness,interference test(triglyceride,bilirubin),auto-correction formula of hematocrit and biological reference interval.Results The whole blood CRP detection system had the blank determination results less than 0.2 mg/L and the carry-over contamination rate being 0.01％.The within-run standard deviations(SDs)of the system for the samples with normal and pathological concentrations were 0.05 and 0.78 mg/L respecti-vely,and the between-run SDs were 0.38 and 1.99 mg/L respectively;the within-run coefficients of variation of the system for the samples with normal and pathological concentrations were 1.05％and 1.61％respectively,and the between-run SDs were 6.83％and 3.95％respectively.The linear range of the system for CRP detection was restricted within 0 and 274.03 mg/L.The system correlated well with BNII speical protein analyzer for CRP detection with r≥0.975.The biases of the system for detecting the reference material were all within±10％.Interference tests showed that the relative deviations of CRP were all within±10％between the samples with or without triglyceride(concentration ≤427.2 mmol/L)and between the samples with or without bilirubin(concentration ≤433 pmol/L).The auto-correction formula of hematocrit proved the system behaved well in linearity.The results of the biological reference interval validation indicated that the CRP values of the samples were well within the existing biological reference intervals and met the requirements.Conclusion The whole blood CRP detection system of BC-7500CS automated hematology analyzer has its performance indexes meet the requirements of the manufacturer and relevant standards,and can be applied clinically.[Chinese Medical Equipment Journal,2024,45(12):55-60]

Objective T o validate the performance of the whole blood C-reactive protein(CRP)detection system of BC-7500CS automated hematology analyzer.Methods The whole blood CRP detection system of BC-7500CS automated hematology ana-lyzer was verified based on relevant industrial standards in terms of blank determination,carry-over contamination rate,precision,linear range,comparability of different detection systems,correctness,interference test(triglyceride,bilirubin),auto-correction formula of hematocrit and biological reference interval.Results The whole blood CRP detection system had the blank determination results less than 0.2 mg/L and the carry-over contamination rate being 0.01％.The within-run standard deviations(SDs)of the system for the samples with normal and pathological concentrations were 0.05 and 0.78 mg/L respecti-vely,and the between-run SDs were 0.38 and 1.99 mg/L respectively;the within-run coefficients of variation of the system for the samples with normal and pathological concentrations were 1.05％and 1.61％respectively,and the between-run SDs were 6.83％and 3.95％respectively.The linear range of the system for CRP detection was restricted within 0 and 274.03 mg/L.The system correlated well with BNII speical protein analyzer for CRP detection with r≥0.975.The biases of the system for detecting the reference material were all within±10％.Interference tests showed that the relative deviations of CRP were all within±10％between the samples with or without triglyceride(concentration ≤427.2 mmol/L)and between the samples with or without bilirubin(concentration ≤433 pmol/L).The auto-correction formula of hematocrit proved the system behaved well in linearity.The results of the biological reference interval validation indicated that the CRP values of the samples were well within the existing biological reference intervals and met the requirements.Conclusion The whole blood CRP detection system of BC-7500CS automated hematology analyzer has its performance indexes meet the requirements of the manufacturer and relevant standards,and can be applied clinically.[Chinese Medical Equipment Journal,2024,45(12):55-60]

OBJECTIVE: To observe the effects of the Xingnao Kaiqiao (regaining consciousness and opening orifices) acupuncture on hemorrhagic transformation and limb motor function after intravenous thrombolysis with recombinant tissue plasminogen activator (rt-PA) in stroke patients. METHODS: A total of 130 stroke patients after rt-PA thrombolytic were divided into an acupuncture group (58 cases, 1 case dropped off) and a non-acupuncture group (72 cases, 7 cases dropped off) according to whether they received acupuncture treatment. Propensity score matching (PSM) was used to match each group, with 38 patients in each group. The patients in the non-acupuncture group received rt-PA thrombolytic therapy and western medical basic treatment. In addition to the basic treatment, the patients in the acupuncture group received Xingnao Kaiqiao acupuncture at Shuigou (GV 26), bilateral Neiguan (PC 6), and ipsilateral Sanyinjiao (SP 6), Chize (LU 5), once a day for 14 days. The incidence of hemorrhagic transformation within 30 days after onset was compared between the two groups. The Fugl-Meyer assessment (FMA) score and activities of daily living (ADL) score were observed at baseline and 30 days, 6 months, 1 year after onset in the two groups. The disability rate at 6 months and 1 year after onset was recorded, and safety was evaluated in both groups. RESULTS: The incidence of hemorrhagic transformation in the acupuncture group was 5.3% (2/38), which was lower than 21.1% (8/38) in the non-acupuncture group (P<0.05). At 30 days, 6 month, and 1 year after onset, the FMA and ADL scores of both groups were higher than those at baseline (P<0.01), and the scores in the acupuncture group were higher than those in the non-acupuncture group (P<0.01). The disability rate in the acupuncture group at 1 year after onset was 10.5% (4/38), which was lower than 28.9% (11/38) in the non-acupuncture group (P<0.05). There was no significant difference in the incidence of adverse events between the two groups (P>0.05). CONCLUSION The Xingnao Kaiqiao acupuncture method could reduce the incidence of hemorrhagic transformation in stroke patients after intravenous thrombolysis with rt-PA, improve their motor function and daily living ability, and reduce the long-term disability rate.
Humans ; Tissue Plasminogen Activator/adverse effects* ; Activities of Daily Living ; Prospective Studies ; Stroke ; Acupuncture Therapy ; Thrombolytic Therapy/adverse effects*

OBJECTIVE: To evaluate the effectiveness and safety of Zhenqi Buxue Oral Liquid (ZQ), progesterone capsules, and their combination in treating oligomenorrhea and hypomenorrhea with qi-blood and Kidney (Shen) essence deficiency. METHODS: This was a prospective, randomized, multi-center controlled trial between June 2022 to December 2022. Ninety-six oligomenorrhea and hypomenorrhea patients with qi-blood and Shen essence deficiency were randomly assigned to receive ZQ (ZQ group, 29 cases), progesterone capsules (PG group, 32 cases), or the combined Chinese and Western medicine (COM group, 31 cases) at a ratio of 1:1:1. Patients in the ZQ or PG group took daily 10 mL twice a day of ZQ or 200 mg once a day of progesterone capsules for 10 consecutive days on day 15 of the menstrual cycle respectively, and patients in the COM group received the same ZQ combined with progesterone capsules. The treatment course lasted for 3 months and follow-up was performed at 1 and 3 months after the end of treatment. Primary endpoint was the menstrual Traditional Chinese Medicine Syndrome Scale (TCMSS) scores. Secondary endpoints included pictorial blood loss assessment chart (PBAC) scores, clinical efficacy rate, 36-item Short Form Health Survey (SF-36) scores, sex hormones and thickness of endometrium. Adverse events (AEs) were recorded. RESULTS: TCMSS scores after 1- and 3-month treatment in all groups were significantly lower than those at baseline (P<0.05). Only TCMSS scores after 3-month treatment in the ZQ and COM groups continuously decreased compared with those after 1-month treatment in the same group (P<0.01). TCMSS scores after 3-month treatment in the ZQ and COM groups were significantly lower than those in the PG group (P<0.05, P<0.01). Compared with baseline, PBAC scores in the ZQ and COM groups after 3 months of treatment were also significantly higher (both P<0.01). The total effective rates of TCM syndrome of 3-month treatment were significantly improved in all groups compared with that after 1 month of treatment (P<0.05). The total effective rate of the COM group was the highest in the 3rd month of treatment and significantly higher than that of PG group alone (P<0.05). Compared with baseline, only the SF-36 scores of COM group were significantly improved after 3 months of treatment (P<0.05). No serious adverse reactions were observed after treatment. CONCLUSIONS The combination of ZQ and PG, or ZQ only had better effects on reducing TCMSS scores compared with PG, and COM showed the higher total effective rate compared with monotherapy. Besides, COM could effectively improve menstrual blood loss and quality of life. ZQ combined with PG may be an effective and safe option for oligomenorrhea and hypomenorrhea patients with qi-blood and Shen essence deficiency.
Female ; Humans ; Progesterone/therapeutic use* ; Qi ; Oligomenorrhea/drug therapy* ; Quality of Life ; Prospective Studies ; Medicine, Chinese Traditional ; Drugs, Chinese Herbal/adverse effects* ; Capsules ; Kidney

INTRODUCTION: We investigated the knowledge, attitudes and practice (KAP) towards coronavirus disease 2019 (COVID-19) and its related preventive measures in Singaporeans aged ≥60 years. METHODS: This was a population-based, cross-sectional, mixed-methods study (13 May 2020-9 June 2020) of participants aged ≥ 60 years. Self-reported KAP about ten symptoms and six government-endorsed preventive measures related to COVID-19 were evaluated. Multivariable regression models were used to identify sociodemographic and health-related factors associated with KAP in our sample. Associations between knowledge/attitude scores and practice categories were determined using logistic regression. Seventy-eight participants were interviewed qualitatively about the practice of additional preventive measures and data were analysed thematically. RESULTS: Mean awareness score of COVID-19 symptoms was 7.2/10. The most known symptom was fever (93.0%) and the least known was diarrhoea (33.5%). Most participants knew all six preventive measures (90.4%), perceived them as effective (78.7%) and practised 'wear a mask' (97.2%). Indians, Malays and participants living in smaller housing had poorer mean scores for knowledge of COVID-19 symptoms. Older participants had poorer attitudes towards preventive measures. Compared to Chinese, Indians had lower odds of practising three out of six recommendations. A one-point increase in score for knowledge and attitudes regarding preventive measures resulted in higher odds of always practising three of six and two of six measures, respectively. Qualitative interviews revealed use of other preventive measures, for example, maintaining a healthy lifestyle. CONCLUSIONS Elderly Singaporeans displayed high levels of KAP about COVID-19 and its related preventive measures, with a positive association between levels of knowledge/attitude and practice. However, important ethnic and socioeconomic disparities were evident, indicating that key vulnerabilities remain, which require immediate attention.
Humans ; Aged ; COVID-19/epidemiology* ; SARS-CoV-2 ; Health Knowledge, Attitudes, Practice ; Cross-Sectional Studies ; Singapore/epidemiology* ; Surveys and Questionnaires

Objective:To summarize the clinical nursing experience of oxygenation (ECMO) adjuvant therapy in children with acute respiratory distress syndrome (ARDS) caused by severe adenovirus pneumonia.Method:The clinical data of 20 children with severe adenovirus pneumonia complicated with ARDS who received ECMO in our hospital from April 2017 to May 2019, including general conditions, pre-treatment, complications and prognosis, were retrospectively analyzed.Results:The primary disease of 20 patients was severe adenovirus pneumonia, and the average duration of ECMO treatment was [247.50(152.00,296.75)] hours. After treatment, 12 (12/20) patients successfully escaped from ECMO, 11 (11/20) patients died, and 9 (9/20) died. Complications occurred in 17 (17/20) patients with ECMO treatment. After discharge from the hospital for six months to two years, 9 patients with good quality of life, social function, normal mental development, 2 patients with ischemia and hypoxia brain damage, in the hospital or family continued rehabilitation exercise.Conclusions:Children with severe adenovirus pneumonia combined with ARDS are critically ill. When other treatments are ineffective, ECMO treatment can provide cardiopulmonary support for children with reversible cardiopulmonary failure, but the complications are numerous and serious. Prevention and reduction of related complications are the key to the success of ECMO.

OBJECTIVE: To investigate the clinical characteristics of essential thrombocytopenia (ET) patients with positive mutations including JAK2, CALR, MPL, or negative mutations. METHODS: A total of 66 newly diagnosed ET cases from January 2016 to December 2018 in Department of Hematology, Huaian No.1 People's Hospital affiliated to Nanjing Medical University were analyzed. Statistical analysis data included the patient's sex, age, symptoms, thrombosis and embolism events, spleen omegaly, platelet count (Plt), leukocyte (WBC) count, hemoglobin (Hb), fibrinogen (FIB), thrombus elastic diagram (TEG), serum potassium, blood glucose (GLU), lactate dehydrogenase (LDH), JAK2, CALR and MPL mutations, treatment options, and efficacy. RESULTS: All the patients were not MPL-positive, and divided in three groups: JAK2 mutation (46 cases, 69.7%), CALR mutation (9 cases, 13.6%) and gene negative mutation (11 cases, 16.7%) group. The average age of patients in the JAK2 mutation group was 63.2 years old, and significantly higher than that in the CALR mutation group (51.8 year) and gene negative group (50.2 year) (P＜0.05). Compared with the JAK2 mutation group and gene negative group, the CALR mutation group had lower WBC count (6.3×10/L vs 13.79×10/L) (P=0.003) (6.3×10/L vs 9.70×10/L) (P=0.009). Also the Hb level of patients in CALR mutation group was lower than the JAK2 mutation group (121.22 g/L vs 136.2 g/L) (P=0.036). However, there was higher tumor burden in the CALR mutation group, compared with the gene negative mutation group (300.11 U/L vs 227.4 U/L) (P=0. 033). There was no significant difference among the three groups, such as the Plt counts, serum potassium level, GLU level and FIB level (P＞0.05). In addition, thrombus and embolism appeared in 30.3% (20/66) cases. 18.2% (12/66) cases were complicated with hyperkalemia, which significantly correlated with Plt counts (r=0.518). TEG was performed in 34 patients, of which 41.2% (14/34) had abnormal TEG and 55.9% (19/34) were accompanied by Plt count ＞ 1 000 ×10/L, but there was no significant correlation between them (r=0.134). After routine clinical treatment, all the 66 cases achieved partial or complete hematological remission, but the disease usually repeated. Until now 4.5% (3/66) cases had been converted to myelofibrosis (MF) all with JAK2 mutation, but without advancing to acute myeloid leukemia. CONCLUSION ET patients with JAK2 mutation have higher incidence, moreover were in older age. However, the patients with CALR mutations display lower WBC count and Hb level, but higher tumor burden. In short, the multiple gene mutations of ET showed different clinical features closely relates with the prognosis, thus providing guidance for the clinical diagnosis and treatment.
Aged ; Calreticulin ; genetics ; Humans ; Janus Kinase 2 ; genetics ; Middle Aged ; Mutation ; Primary Myelofibrosis ; Thrombocythemia, Essential ; Thrombocytopenia

Objective:To compare the total daily doses of 16 active components in big honeyed pills, concentrated pills and tablets of Fuzi Lizhongwan. Method:Three dosage forms of Fuzi Lizhongwan were prepared according to the process described in the literature. RRLC-QqQ-MS was employed to analyze the contents of 16 active ingredients with mobile phase of 0.1%formic acid aqueous solution-0.1%formic acid acetonitrile solution for gradient elution,the separation was performed on a Accucore RP-MS column(2.1 mm×100 mm, 2.6 μm) with a flow rate of 0.3 mL·min^-1 and the column temperature at 30℃, the mass spectrometry condition was electrospray ion source, positive and negative ion switching mode for detection, multi-reaction monitoring mode(MRM) for scanning. The contents of 16 active ingredients were calculated, and the normalization arithmetic method was used for comparing the total daily doses of these active ingredients in three dosage forms of Fuzi Lizhongwan. Result:Processed products of Aconiti Lateralis Radix Praeparata were used as raw powder in preparation process of the three dosage forms, so there was no significant difference in the contents of six alkaloids in the three dosage forms, while the contents of other 10 active ingredients from Zingiberis Rhizoma, Codonopsis Radix, Atractylodis Macrocephalae Rhizoma and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle were significantly higher in big honeyed pills than those in concentrated pills or tablets(P<0.01), due to the differences in the extracting solvents(ethanol and water) and ethanol concentration, the contents of other 10 active ingredients between concentrated pills and tablets were also significant differences. The total daily doses of these 16 active components were 18.764, 17.530, 5.676 mg in big honeyed pills, concentrated pills and tablets, respectively. The concentrated pills exhibited the highest normalization arithmetic score of 0.717 5 compared with 0.605 4 and 0.312 4 separately in big honeyed pills and tablets, it was mainly owing to the maximum amount of raw material per day in concentrated pills. Conclusion:The total daily doses of 16 active ingredients in the three dosage forms of Fuzi Lizhongwan are significantly different caused by preparation process, prescription and dosage.

Objective To analyze statistically the author population in the issues of the Chinese Journal of Nautical Medicine and Hyperbaric Medicine (hence termed as the Chinese Nautical Medicine) from 2006 to 2011,with the aims to offer contributors constructive advices and give reference and basic data to the medical information service divisions and to those who run or evaluate the Journal.Methods Statistical analyses were made on the authors,authors' institutions,cooperative rate,authors region distribution in the papers of the issues published from 2006 to 2011.Results (1) The papers published in the issues of the Journal from 2006 to 2011 were 1062,involving 28 provinces,autonomous regions and municipalities directly under the central government.Mainland provinces except Qinghai all had their papers published in the Journal.Such cities and province as Shanghai,Beijing and Shandong were the top 3 contributing regions,accounting for 53.38％ of the total contributing amount.(2) The 1062 papers published in the issues were respectively from 322 institutions.The number of papers published in the Journal by the top 10 contributing institutions during this period were 322,accounting for 42.09％.The 1062 papers were contributed by 785 authors (first authors),of whom 593 were instantaneous author (published only one paper),accounting for 75.54％.The number of authors who published papers no less than 3 papers was 55,accounting for 7.01％.(3)The cooperative rate of coauthors was 91.15％,with an average cooperative rate of 4.34.From the analysis of contributing corporate institutions,the average co-institution rate was 29.66％,with an average co-institution rate of 1.39.Conclusions In the fields of nautical medicine and hyperbaric medicine,the Journal was quite influential and attractive to readers,and it also showed that the authors and authors' institutions of the Journal displayed a rather good cooperative spirit.

Objective To analyze statistically the author population in the issues of the Chinese Journal of Nautical Medicine and Hyperbaric Medicine (hence termed as the Chinese Nautical Medicine) from 2006 to 2011,with the aims to offer contributors constructive advices and give reference and basic data to the medical information service divisions and to those who run or evaluate the Journal.Methods Statistical analyses were made on the authors,authors' institutions,cooperative rate,authors region distribution in the papers of the issues published from 2006 to 2011.Results (1) The papers published in the issues of the Journal from 2006 to 2011 were 1062,involving 28 provinces,autonomous regions and municipalities directly under the central government.Mainland provinces except Qinghai all had their papers published in the Journal.Such cities and province as Shanghai,Beijing and Shandong were the top 3 contributing regions,accounting for 53.38％ of the total contributing amount.(2) The 1062 papers published in the issues were respectively from 322 institutions.The number of papers published in the Journal by the top 10 contributing institutions during this period were 322,accounting for 42.09％.The 1062 papers were contributed by 785 authors (first authors),of whom 593 were instantaneous author (published only one paper),accounting for 75.54％.The number of authors who published papers no less than 3 papers was 55,accounting for 7.01％.(3)The cooperative rate of coauthors was 91.15％,with an average cooperative rate of 4.34.From the analysis of contributing corporate institutions,the average co-institution rate was 29.66％,with an average co-institution rate of 1.39.Conclusions In the fields of nautical medicine and hyperbaric medicine,the Journal was quite influential and attractive to readers,and it also showed that the authors and authors' institutions of the Journal displayed a rather good cooperative spirit.