OBJECTIVETo explore a safe and effective method for the treatment of low back pain in the cutaneous nerve, and to clarify the indication of Pi needle to treat it.

METHODSFrom January 2003 to December 2004, 278 patients with cutaneous nerve entrapment low back pain were divided into two groups: Pi needle group and electrical stimulation group. In the Pi needle group, there were 68 males and 70 females, ranging in age from 20 to 60 years old, with an average of(41.92±10.88)years old. In the electrical stimulation group, there were 68 males and 72 females, ranging in age from 18 to 60 years old, with an average of(41.44±10.47) years old. The pain, tenderness and soft tissue tension of the two groups were measured and compared before and after treatment.

RESULTSAll of the selected cases were qualified. No suspension, culling and shedding cases occurred in either group. In Pi needle group, visual analog scale(VAS) of pain decreased from 8.78±1.52 before treatment to 1.33±1.33 after treatment;and in electrical stimulation group, VASof pain decreased from 8.59±1.76 before treatment to 5.20±2.64 after treatment;and the VAS of pain of the Pi needle group was lower than that of the electrical stimulation group. In Pi needle group, VAS of tenderness decreased from 9.12±1.24 before treatment to 1.60±1.36 after treatment;and in electrical stimulation group, VAS of pain decreased from 8.79±1.60 before treatment to 5.34±2.60 after treatment;and the VAS of pain of the Pi needle group was lower than that of the electrical stimulation group.

CONCLUSIONSOnce tissue texture changes to pain point, cord, nodules, Pi needle is the first line treatment for the cutaneous nerve entrapment low back pain.

The purpose of this research is to investigate the effects and mechanisms of paeonol (PL), a phenolic compound found in many traditional Chinese formulations, on reversing drug resistance in the ovarian cancer resistant SKOV3/DDP cells. The results showed that PL had significant drug-resistant reversal effect on SKOV3/DDP cells. Flow cytometry showed that PL could inhibit P-glycoprotein (P-gp) function in a concentration-dependent manner. Fluorescent quantitative PCR and cell immunofluorescence techniques were used to detect mechanisms of action. Results revealed that both the inhibitory effect on MDR1/P-gp and metadherin (MTDH) expression and the induction effect on phosphatase and tensin homolog (PTEN), by 15, 30, and 60 μmol·L^-1 PL, were increased with increased concentrations of PL (P < 0.01, P < 0.05). The inhibitory effect on MTDH mRNA and the induction effect on PTEN mRNA, by PI3K inhibitor LY294002, were stronger or equivalent to that of the 60 μmol·L^-1 PL treated group; however, the inhibition or induction effect on MTDH or PTEN protein were only comparable to the 15 μmol·L^-1 PL treated group. The present study shows that the effect of PL on SKOV3/DDP cells may be related to the inhibition of P-gp function and expression, the inhibition of MDR1, MTDH expression, and the induction of PTEN expression, all which can provide a theoretical foundation for PL as a drug resistance reversal agent on the treatment of ovarian cancer chemotherapy resistance.

Objective To investigate the effects of HP and PHP maneuver training on human centrifuge for the purpose of establishing a scientific training approach on centrifuge. Methods According to the maneuverability of high performance fighter aircrafts,a 3-day centrifuge training program was constituted.The highest training G level was 6.0,7.0,9.0 G respectively for 3 training days.On the 1st day,preprogrammed acceleration profiles were adopted.After relaxed G tolerance test,pilots who wore anti-G suit or compensatory suit underwent HP maneuver training.On the 2nd and 3rd day,trainings of HP breathing were carried out during 3.0 G exposures.The close-loop controlled trainings were conducted during 5.0,7.0,8.0 and 9.0 G runs where pilots were performing HP and PHP maneuver.Fifty-five pilots went through the centrifuge trainings.Changes of +Gz tolerance,HR,breathing rate (BR),and muscular force of legs (Flegs) during the training were observed and analyzed. Results Fifty-two of the 55 pilots (94.55％) completed the training of 8.0 G for 10 s with the elevated G tolerance ranging from 2.0 G to 3.0 G.Flegs and HR raised significantly with the increment of G level (F =111.95-1253.21,P＜ 0.01).BR was controlled between 20-26 times/min.The trainees reported that the close-loop controlled training was more comfortable and preferred than passive controlled training. Conclusions The HP and PHP maneuver training approach on centrifuge could enhance pilots anti-G capability greatly,and thus the aerial combat performance of pilots.

Objective To investigate the difference of + Gz tolerance between male and female student pilots by testing the forces developed by respiratory and leg muscles,and to verify the G tolerance test by means of anti-G training apparatus. Methods Thirty-two female and thirty male student pilots volunteered for this study.The vertical heart-to-eye distance,maximum expiration pressure,maximum inspiration pressure and forces developed by leg muscles,systolic blood pressure when performing HP and PHP maneuver were measured.The + Gz tolerance was reckoned by an empirical formula when volunteers riding on anti-G training apparatus with the performance of straining maneuver and anti-G suit. Results The vertical heart-to-eye distance,maximum expiration pressure,maximum inspiration pressure and forces developed by leg muscles of male student pilots were significantly greater than those of the females (t=4.852～6.762,P＜0.01).The G tolerance of male students in performing PHP maneuver was greater than that of the females (t=2.226,P=0.034).There was no significant difference in G tolerance when performing HP maneuver between female and male student pilots (P =0.185). Conclusion ① The respiratory and leg muscles of female student pilots are not as strong as male student pilots'.② Female student pilots may beneficially gain G tolerance due to their shorter heart-to-eye distance,but they could reach relative lower systolic blood pressure when performing HP maneuver.Such resulted effects maybe the important reason of causing female' s inapparent G tolerance improvement comparing to the male' s.Because of their weaker respiratory muscles,the female students do not accommodate themselves to PBG (positive pressure breathing for G) and show poorer G tolerance as they performing PHP maneuver with PBG.③ The blood pressure measurement when performing AGSM (anti-G straining maneuver) need to be improved for getting better success rate and accurateness.

Objective To investigate the effects of HP and PHP maneuver training on human centrifuge for the purpose of establishing a scientific training approach on centrifuge. Methods According to the maneuverability of high performance fighter aircrafts,a 3-day centrifuge training program was constituted.The highest training G level was 6.0,7.0,9.0 G respectively for 3 training days.On the 1st day,preprogrammed acceleration profiles were adopted.After relaxed G tolerance test,pilots who wore anti-G suit or compensatory suit underwent HP maneuver training.On the 2nd and 3rd day,trainings of HP breathing were carried out during 3.0 G exposures.The close-loop controlled trainings were conducted during 5.0,7.0,8.0 and 9.0 G runs where pilots were performing HP and PHP maneuver.Fifty-five pilots went through the centrifuge trainings.Changes of +Gz tolerance,HR,breathing rate (BR),and muscular force of legs (Flegs) during the training were observed and analyzed. Results Fifty-two of the 55 pilots (94.55％) completed the training of 8.0 G for 10 s with the elevated G tolerance ranging from 2.0 G to 3.0 G.Flegs and HR raised significantly with the increment of G level (F =111.95-1253.21,P＜ 0.01).BR was controlled between 20-26 times/min.The trainees reported that the close-loop controlled training was more comfortable and preferred than passive controlled training. Conclusions The HP and PHP maneuver training approach on centrifuge could enhance pilots anti-G capability greatly,and thus the aerial combat performance of pilots.

Objective To investigate the difference of + Gz tolerance between male and female student pilots by testing the forces developed by respiratory and leg muscles,and to verify the G tolerance test by means of anti-G training apparatus. Methods Thirty-two female and thirty male student pilots volunteered for this study.The vertical heart-to-eye distance,maximum expiration pressure,maximum inspiration pressure and forces developed by leg muscles,systolic blood pressure when performing HP and PHP maneuver were measured.The + Gz tolerance was reckoned by an empirical formula when volunteers riding on anti-G training apparatus with the performance of straining maneuver and anti-G suit. Results The vertical heart-to-eye distance,maximum expiration pressure,maximum inspiration pressure and forces developed by leg muscles of male student pilots were significantly greater than those of the females (t=4.852～6.762,P＜0.01).The G tolerance of male students in performing PHP maneuver was greater than that of the females (t=2.226,P=0.034).There was no significant difference in G tolerance when performing HP maneuver between female and male student pilots (P =0.185). Conclusion ① The respiratory and leg muscles of female student pilots are not as strong as male student pilots'.② Female student pilots may beneficially gain G tolerance due to their shorter heart-to-eye distance,but they could reach relative lower systolic blood pressure when performing HP maneuver.Such resulted effects maybe the important reason of causing female' s inapparent G tolerance improvement comparing to the male' s.Because of their weaker respiratory muscles,the female students do not accommodate themselves to PBG (positive pressure breathing for G) and show poorer G tolerance as they performing PHP maneuver with PBG.③ The blood pressure measurement when performing AGSM (anti-G straining maneuver) need to be improved for getting better success rate and accurateness.

ObjectiveTo study the significance of Double Therapy Treatment Based On Differentiation Of Syndromes on obsession with obsessive thoughts but without compulsive behavior and its suitability in open wards.Methods47 cases were divided into experimental group(24 cases) and control group(23 cases) randomly in open wards. Experimental group was treated by Double Therapy Treatment Based on Differentiation of Syndromes while control group by Cognitive Therapy combined with Chlorimpramine and Fluoxetine etc. The period of treatment was 5 months, in which positive therapy cost 2 months and free therapy cost next 3 months. Y-BOCS was used to value the effect.ResultsThe recovery rate in experimental group was much higher than that of the control group 2 months (χ2=12.44,P<0.01) and 5 months (χ2=18.00,P<0.01)later. There was significant difference in Y-BOCS score between these two groups 2 months (t=7.140,P<0.001) and 5 months (t=8.191,P<0.001)later.ConclusionDouble Therapy Treatment Based On Differention Of Syndromes shows a satisfied curative effect and prognosis on obsession with obsessive thoughts but without compulsive behavior and should be advocated.

OBJECTIVETo investigate the mechanism and the suppression effect of human cytomegalovirus (HCMV) on hematopoietic system.

METHODSSemi-solid culture system was used to observe the effect of HCMV AD169 strain on colony forming unit granulocyte/macrophage (CFU-GM), CFU-erythroid (CFU-E), CFU-multipotent (CFU-Mix) and CFU-megakaryocyte (CFU-MK) growth. The techniques of in situ polymerase chain reaction (IS-PCR) and polymerase chain reaction (PCR) were used to demonstrate the existence of HCMV DNA in the colony cells of cultured CFU-GM, CFU-Mix, CFU-MK and CFU-E, respectively. The immediate early antigen (IEA) mRNA in CFU-MK and late antigen (LA) mRNA in CFU-E were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). HCMV early protein P52 was detected with immunohistochemical technique.

RESULTSHCMV AD169 suppressed the differentiation and proliferation of CFU-GM, CFU-E, CFU-Mix and CFU-MK in vitro significantly (P < 0.05). The suppression was dose-dependent. HCMV DNA was successfully detected in CFU-GM, CFU-Mix, CFU-MK colony cells from viral infection groups by IS-PCR, and was detected in CFU-E by PCR, while it was negative in blank control or mock control groups. CFU-MK colony cells expressed HCMV IEA mRNA with the size of 340 bp in virus infection groups of 10(3) plague forming unit (PFU), 10(4) PFU and 10(5) PFU, respectively. The HCMV LA mRNA was detected by RT-PCR and was 263 bp long in positive control group of HCMV-infected human embryonic fibroblasts. The expression of HCMV LA mRNA in CFU-E was negative. The early protein P52 of HCMV in 10(4) PFU group was also identified by immunohistochemical staining.

CONCLUSIONHCMV AD169 strains inhibited the differentiation and proliferation of CFU-GM, CFU-E, CFU-Mix and CFU-MK by the infection of the hematopoietic progenitors. HCMV might cause the suppression of hematopoiesis by direct infection, which is thought to be one of the reasons of HCMV infection associated with thrombocytopenia, neutropenia and anemia.

Colony-Forming Units Assay ; Cytomegalovirus ; genetics ; DNA, Viral ; genetics ; Erythrocytes ; virology ; Hematopoietic System ; cytology ; virology ; Humans ; Megakaryocytes ; virology ; Multipotent Stem Cells ; virology ; Polymerase Chain Reaction