ObjectiveTo investigate the preventive and therapeutic effects of Qingfei Paidu decoction (QFPDD) on acute lung injury (ALI) in mice and its underlying molecular mechanisms based on miRNA sequencing technology. MethodsTwenty-four 4-week-old male KM mice were randomly divided into a control group, a model group, and a QFPDD group (n = 8 per group). After one week of acclimatization, mice in the control and model groups were intragastrically administered ultrapure water (0.2 mL per dose), whereas mice in the QFPDD group were intragastrically administered QFPDD (1.6 g crude drug/mL, 0.2 mL per dose), twice daily for 8 consecutive days. On days 2–8, mice in the model and QFPDD groups were exposed to aerosolized lipopolysaccharide (LPS) solution (2.5 g/L, 4 mL per exposure) for 7 consecutive days. On day 9, blood was collected via the retro-orbital venous plexus under deep anesthesia, and lung tissues were harvested. Body weight and lung weight were measured, and the lung coefficient was calculated. Serum levels of inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6 were detected by ELISA. Lung histopathological changes were observed by HE staining of paraffin-embedded sections. miRNA expression profiles in lung tissues were analyzed using the Illumina HiSeq 2500 sequencing platform. Target genes of differentially expressed miRNAs were predicted using bioinformatics databases, and functional enrichment analysis of these target genes was performed using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Differentially expressed miRNAs were validated by reverse transcription quantitative real-time PCR (RT-qPCR). ResultsCompared with the control group, the model group showed a consistent body weight growth trend but a significantly increased lung coefficient (P < 0.01). ELISA results showed that serum levels of TNF-α and IL-6 were significantly elevated in the model group compared with the control group (P < 0.01), whereas QFPDD treatment significantly reduced serum TNF-α and IL-6 levels compared with the model group (P < 0.05). HE staining showed that, compared with the control group, the model group exhibited widened alveolar septa, massive inflammatory cell infiltration, partial alveolar expansion, and mild capillary dilation with congestion. In contrast, the QFPDD group showed only slightly widened alveolar septa and mild inflammatory cell infiltration compared with the model group. Intersection analysis of miRNA sequencing data identified 13 differentially expressed miRNAs common to both the model vs. control and QFPDD vs. model comparisons. Among them, 6 miRNAs (mmu-miR-203-3p, mmu-miR-181b-5p_R-1, hsa-miR-4286_R+1, mmu-miR-1843b-5p_L+1R-1_2, mmu-miR-22-3p, and mmu-miR-1964-3p) were significantly up-regulated in the model group (P < 0.05) and significantly down-regulated after QFPDD treatment (P < 0.05), showing a therapeutic reversal trend. GO analysis revealed that the target genes of the differentially expressed miRNAs were mainly enriched in biological processes such as RNA polymerase Ⅱ transcriptional regulation. KEGG analysis indicated that target genes were mainly enriched in signaling pathways including the mitogen-activated protein kinase (MAPK) pathway. RT-qPCR validation result for mmu-miR-203-3p was consistent with the sequencing analysis results. ConclusionQFPDD may exert preventive and therapeutic effects against ALI by regulating the expression of mmu-miR-203-3p and other miRNAs, thereby modulating inflammatory responses and the MAPK signaling pathway and participating in the pathological process of lung injury.

Current experimental and computational methods have limitations in accurately and efficiently classifying ion channels within vast protein spaces. Here we have developed a deep learning algorithm, GPT2 Ion Channel Classifier (GPT2-ICC), which effectively distinguishing ion channels from a test set containing approximately 239 times more non-ion-channel proteins. GPT2-ICC integrates representation learning with a large language model (LLM)-based classifier, enabling highly accurate identification of potential ion channels. Several potential ion channels were predicated from the unannotated human proteome, further demonstrating GPT2-ICC's generalization ability. This study marks a significant advancement in artificial-intelligence-driven ion channel research, highlighting the adaptability and effectiveness of combining representation learning with LLMs to address the challenges of imbalanced protein sequence data. Moreover, it provides a valuable computational tool for uncovering previously uncharacterized ion channels.

Current experimental and computational methods have limitations in accurately and efficiently classi-fying ion channels within vast protein spaces.Here we have developed a deep learning algorithm,GPT2 Ion Channel Classifier(GPT2-ICC),which effectively distinguishing ion channels from a test set con-taining approximately 239 times more non-ion-channel proteins.GPT2-ICC integrates representation learning with a large language model(LLM)-based classifier,enabling highly accurate identification of potential ion channels.Several potential ion channels were predicated from the unannotated human proteome,further demonstrating GPT2-ICC's generalization ability.This study marks a significant advancement in artificial-intelligence-driven ion channel research,highlighting the adaptability and effectiveness of combining representation learning with LLMs to address the challenges of imbalanced protein sequence data.Moreover,it provides a valuable computational tool for uncovering previously uncharacterized ion channels.

Objective To identify easily confused Chinese herbal pieces by micro-macroscopical identification method,and study its application value in quality acceptance of Chinese herbal pieces in medical institutions.Methods Using the method of micro-macroscopical identification,with the help of related instruments,the microscopic characteristics of 10 groups of 31 kinds of Chinese herbal pieces,which were easy to be confused and could not be observed by naked eye and ordinary magnifying glass,were analyzed and summarized according to the classification of different medicinal parts,such as flowers,fruits,seeds,whole grass,roots and rhizomes,stems,and others,and pictures were taken to save them.Results The micro-characters of 10 groups of 31 easily confused Chinese herbal medicines,including Eriocauli flos,Eriocaulon sexangulare,Eriocaulon australe,Aurantii fructus,Citrus changshan-huyou,Chaenomelis fructus,Chaenomeles cathayensis,Perillae fructus,Perillae fructus(Physalis alba),Perillae fructus(Wild),Astragali complanati semen,Astragalus chinensis,Selaginellae herba,Selaginella pulvinata,Selaginella moellendorfii,Selaginella doederleinii,Selaginella uncinata,Lysimachiae herba,Lysimachia hemsleyana,Desmodium styracifolium,Dryopteris crassirhizoma,Woodwardia japonica,Brainea insignis,root of Valvate actinidia,Actinidia arguta,Tripterygium wilfordii,root of Romanet grape,Trogopterus dung,Bat dung,Hare dung and Silkworm sand were observed.The various traditional Chinese medicines possessed unique identification characteristics.Flower-based medicines could be studied for their color,tissue structure,and surface features such as texture and trichomes.Fruit-based medicines could be studied for their fruit peel structure,color,degree of development,and outer characteristics such as cuticle layer and wax.Seed-based medicines could be studied for their morphology,size,color,and texture.Whole plant-based medicines could be studied for leaf length-to-width ratio,widest point,surface characteristics,and splitting situation,as well as stem trichomes and stomata.Root-based medicines,rhizome-based medicines,and stem-based medicines could be studied for their bark,wood structure,and the morphology and arrangement of vascular bundles or ducts.Other kinds of medicines could be studied from their sources,and these unique micro-characteristic identification features could be easily discovered with the help of a microscope.Conclusion Compared with the traditional characteristic identification,micro-macroscopical identification is more capable of improving the accuracy of Chinese herbal pieces identification,which is economical,practical,simple and intuitive,and can be popularized as a commonly used identification method of herbal medicines in medical institutions,and it is a feasible solution to realize the rapid inspection of herbal medicines.

Objective:To explore the effect of continuous nursing on the nutritional status, muscle strength, physical activity function, quality of life, and degree of weakness of elderly patients.Methods:120 cases of elderly debilitated patients admitted in Beijing Friendship Hospital from June 2019 to July 2020 were selected and divided into a control group and a continuous care group according to the random number table method, with 60 cases in each group. The control group was given routine nursing, and the continuing nursing group was given continuous nursing. The cumulative frailty index (FI); the score of grip strength, timed up and go test (TUGT), short physical performance battery (SPPB); body mass index (BMI), serum albumin level and quality of life score of the two groups were compared.Results:After the intervention, the FI score, SPPB score and overall health score of the continuous nursing group were 0.25±0.05, 9.35±1.36, 68.22±6.32 respectively, and those of the control group were 0.36±0.12, 7.55±1.18, 60.56±6.38 respectively, the differences between the two groups were statistically significant ( t value was -6.554, 7.744, 6.607, P<0.05); after the intervention, the grip strength, TUGT level, BMI and serum albumin level in the continuous nursing group were (28.36±1.52) kg, (15.34±2.14) s, (21.15±0.95) kg/m 2, (36.85±3.92) g/L, respectively, while those in the control group were (25.22±1.78) kg, (19.56±2.68) s, (19.28±1.18) kg/m 2, (32.18±4.82) g/L, and the differences between the two groups were statistically significant ( t value was -9.531-10.391, P<0.05). Conclusion:Continuous nursing can effectively ameliorate the nutritional status of elderly debilitated patients, improve muscle strength, physical activity and quality of life, and delay the debilitating process.

Objective:To explore the effect of food intake management based on swallowing safety assessment in elderly patients with frailty.Methods:From April 2020 to March 2021, convenience sampling was used to select 126 elderly patients with frailty in the Department of Geriatrics, Beijing Friendship Hospital, Capital Medical University, as the research object. According to the random number table method, the patients were divided into the observation group and the control group, with 63 cases in each group. The control group implemented routine food intake management, and the observation group carried out food intake management based on swallowing safety assessment for a period of 12 weeks. The nutritional indicators, incidence of aspiration, quality of life and frailty status of the two groups of patients were compared.Results:After the intervention, the hemoglobin, serum total protein, serum albumin levels and the Short Form 36 Health Survey Questionnaire (SF-36) scores of the observation group were higher than those of the control group, and higher than this group before the intervention, and the differences were statistically significant ( P<0.05) . The incidence of aspiration in the observation group and the control group were 11.11% (7/63) and 33.33% (21/63) respectively, and the difference was statistically significant ( P<0.05) . Conclusions:Food intake management based on swallowing safety assessment can effectively improve the nutritional status and quality of life of elderly patients with frailty, and reduce the incidence of aspiration.

Objective To observe the clinical effects of auricular plaster therapy combined with acupoint application on preventing postpartum retention of urine. Methods From June 2016 to October 2016, we selected 240 puerpera with full-term pregnancy of Department of Gynaecology and Obstetrics in Hangzhou Red Cross Hospital by random sampling. All of the patients were divided into four groups by the random number table, 60 cases in each group. Patients in control group were treated with routine postpartum care. In this basis, patients in auricular plaster therapy group accepted auricular plaster therapy, patients in acupoint application group accepted acupoint application therapy, patients in auricular plaster therapy & acupoint application group were treated with auricular plaster therapy and acupoint application therapy. Finally, the time of the first micturition, bladder residual urine volume (RUV) and clinical effects of puerpera were compared in four groups. Results The time of the first micturition in auricular plaster therapy & acupoint application group (combined group), auricular plaster therapy group, acupoint application group and control group was (2.45±0.51), (4.28±1.23), (4.25±1.15) and (5.60±1.75) h respectively with significant differences (F= 65.207, P<0.001). The bladder RUV of auricular plaster therapy & acupoint application group was (52.10±12.51) ml less than those of other three groups. The total effective rate of auricular plaster therapy & acupoint application group was 100.00% high than that of other three groups with a significant difference (χ2=179.770, P<0.001). Compared with control group, the time of the first micturition in auricular plaster therapy & acupoint application group was shorter, the bladder RUV was all less, the total effective rate was higher with significant differences (P<0.05). There was no statistically significant difference between auricular plaster therapy group and acupoint application group (P>0.05). Conclusions The auricular plaster therapy combined with acupoint application can effectively prevent postpartum retention of urine which is worth to be spread.

Objective To study the psychological intervention combined with ritodrine hydrochloride and observe the effect of Magnesium Sulfate threatened abortion after 20 weeks of treatment. Methods 100 pregnant women with threatened abortion in our hospital from February 2015 to October 2016 were selected and randomly divided into control group and experimental group. Each group had 50 cases. the control group was treated with Magnesium Sulfate treatment, experimental group was treated with ritodrine hydrochloride treatment, psychological intervention, pay attention to mental health, strengthen the communication with patients. Results After the corresponding treatment, the SAS score of the experimental group was (40.1±6.2) points, and the SDS score was (38.4±7.9) points. The scores of SAS and SDS in the control group were (49.6±7.2) points, (48.2±8.5) points. After treatment, 5 patients in the experimental group had adverse reactions, and the incidence of adverse reactions was 10%. Adverse reactions occurred in 13 patients in the control group. The adverse reaction rate in the control group (26.0%) was significantly higher than that in the experimental group (10.0%), with statistical difference (P<0.05). The weight of the newborn, the duration of pregnancy and the onset time of the drug in the experimental group were better than those in the control group (P<0.05). Conclusion Psychological intervention combined with ritodrine hydrochloride and Magnesium Sulfate have good clinical effect on the treatment of threatened abortion after 20 weeks of gestation, can visibly improve patients' depression and anxiety, with further clinical promotion and application significance.

Objective To investigate the effect of progesterone combined with psychological intervention on threatened abortion. Methods According to the different treatment and nursing intervention mode selected from be January 2015 to January 2017 in our hospital 60 cases of patients with threatened abortion group: the control group used dydrogesterone + routine nursing intervention, the observation group with psychological nursing mode + progesterone intervention; the two groups of patients with clinical symptoms, the incidence of adverse reactions and nursing satisfaction were recorded in detail, and the relevant data for comparative analysis. Results The psychological nursing mode of progesterone +(observation group) intervention is better than the clinical effect of patients with threatened abortion and dydrogestrone + routine nursing (control group) the clinical intervention effect, clinical symptoms improved better than the control group, the incidence rate of adverse reaction was lower than the control group, nursing satisfaction was higher than the control group, the difference was statistically significant (P<0.05). Conclusion Threatened abortion patients choose progesterone + psychological intervention effect significantly, can effectively improve the clinical symptoms and adverse reactions and nursing satisfaction, is widely used in clinical intervention of threatened abortion patients.

Objective To investigate the effects of labetalol, Magnesium Sulfate, psychological intervention combined with the clinical effect of treatment of gestational hypertension. Methods According to the different treatment and nursing intervention mode of 120 cases of hypertension patients in January 2015 to 2017 year in January in our hospital for diagnosis and treatment of pregnancy groups: control group with routine nursing intervention in the observation group, Magnesium Sulfate +, Magnesium Sulfate + + mode labetalol psychological nursing intervention; the detailed records of the two groups of patients with clinical curative effect, complications, etc. the situation, and the related data for comparative analysis of labetalol, Magnesium Sulfate, psychological intervention combined with the clinical effect of treatment of gestational hypertension. Results The psychological nursing mode of labetalol + Magnesium Sulfate (observation group) intervention effect in patients with hypertension during pregnancy is better than the Magnesium Sulfate + routine nursing (control group) the clinical intervention effect, clinical symptoms improved better than the control group, the complication rate was lower than the control group, nursing satisfaction was higher than the control group, the difference was statistically significant (P<0.05). Conclusion Patients with gestational hypertension selection effect of labetalol + Magnesium Sulfate +psychological nursing intervention significantly, can effectively improve the clinical symptoms and reduce the incidence of complications.