Objective To investigate the role of MBP-1 in proliferation,apoptosis and invasion of human esophageal cancer cells Ec109.Methods The human esophageal cancer cells Ec109 were cultured,and divided into the MBP-1 mimics group,siRNA-MBP-1 group,negative control group and blank control group.The cell proliferation activity of each group was detected by tetrazolium blue (MTT) method;flow cytometry was used to detect cell apoptosis and cell cycle;Transwell assay was used to detected the invasion ability and the expressions of cellular cycle related C-myc,Cyclin D1 and Cyclin E were detected by western blot.Results Compared with the negative control group and blank control group,the expression of MBP-1 in esophageal cancer cells Ec109 of the MBP-1 mimics group was up-regulated (P＜0.05),the proliferation ability of esophageal cancer cells was decreased,increased the proportion of apoptosis,decreased the proportion of G0/G1 phase cells,inhibited the number of invasive cells was decreased and the expressions of C-myc,Cyclin D1 and Cyclin E proteins.After silencing MBP-1,the expression of MBP-1 in esophageal cancer cells Ec109 in the siRNA-MBP-1 group was down-regulated,the proliferation ability of esophageal cancer cells was increased,the proportion of apoptosis was decreased,the proportion of G0/G1 phase cells was increased.the number of invasive cells was increased and the expressions of C-myc,Cyclin D1 and Cyclin E proteins were up-regulated.Conclusion MBP-1 is closely correlated with the cell proliferation,cell apoptosis and invasion ability of human esophageal cancer cell line Ec109,and its mechanism might be related to cell cycle abnormality.

Objective To investigate the prevention effect of nursing intervention on contrast-induced nephropathy in lower extremity atherosclerotic occlusive disease patients treated by interventional therapy.Methods A total of 100 lower extremity atherosclerotic occlusive disease patients treated by interventional therapy were randomly divided into two groups.The control group (n=50) was given routine nursing care, while the observation group (n=50) received preventive nursing to reduce contrast-induced nephropathy on the basis of the control group.The changes of renal function and the incidence of contrast-induced nephropathy were compared between the two groups.Results There was no significant difference in BUN between the observation group and the control group (P>0.05).However, the level of plasma cystatin (CysC) in the observation group was higher, and levels of serum creatinine (Scr) and urinary protein were lower than that in the control group, the differences were statistically significant (P<0.05).Incidence of contrast-induced nephropathy in the observation group was lower than that in the control group, the difference was statistically significant (P<0.05).Conclusion Preventive nursing can reduce renal damage in the interventional therapy of low arteriosclerosis, reduce the incidence of contrast-induced nephropathy.

Objective To investigate the prevention effect of nursing intervention on contrast-induced nephropathy in lower extremity atherosclerotic occlusive disease patients treated by interventional therapy.Methods A total of 100 lower extremity atherosclerotic occlusive disease patients treated by interventional therapy were randomly divided into two groups.The control group (n=50) was given routine nursing care, while the observation group (n=50) received preventive nursing to reduce contrast-induced nephropathy on the basis of the control group.The changes of renal function and the incidence of contrast-induced nephropathy were compared between the two groups.Results There was no significant difference in BUN between the observation group and the control group (P>0.05).However, the level of plasma cystatin (CysC) in the observation group was higher, and levels of serum creatinine (Scr) and urinary protein were lower than that in the control group, the differences were statistically significant (P<0.05).Incidence of contrast-induced nephropathy in the observation group was lower than that in the control group, the difference was statistically significant (P<0.05).Conclusion Preventive nursing can reduce renal damage in the interventional therapy of low arteriosclerosis, reduce the incidence of contrast-induced nephropathy.

OBJECTIVETo investigate the safety and efficiency of the disposable circumcision suture device (DCSD) in the surgical treatment of phimosis and redundant prepuce.

METHODSWe randomly assigned 249 outpatients with phimosis or redundant prepuce to be treated with DCSD (n = 129) and by conventional circumcision (CC, n = 120), respectively. Then we compared the safety and efficiency of the two strategies.

RESULTSComparisons between DCSD and CC showed that the operation time was (4.02 +/- 0.69) vs (30.8 +/- 4.05) min, blood loss was (1.07 +/- 1.29) vs (8.72 +/- 2.15) ml, intraoperative pain score was 0.81 +/- 0.81 vs 2.42 +/- 1.15, 24-hour postoperative pain score was 1.84 +/- 1.02 vs 4.99 +/- 1.36, postoperative complication rate was 13. 95% (18/129) vs 9.17% (11/120), wound healing time was (13.99 +/- 9.06) vs (17.48 +/- 3.49) d, satisfaction with the penile appearance was 98.4% (127/129) vs 95% (109/120), and treatment cost was (2215.62 +/- 17.67) vs (576.47 + 15.58) Y RMB. DCSD exhibited obvious superiority over CC for shorter operation time, less blood loss, milder intraoperative pain, sooner wound healing, and better penile appearance, but it also had a higher rate of postoperative complications (P > 0.05) and involved more treatment cost than the latter (P < 0.05).

CONCLUSIONThe disposable circumcision suture device affords ideal clinical effects and therefore deserves clinical popularization.

Circumcision, Male ; instrumentation ; Disposable Equipment ; Follow-Up Studies ; Humans ; Male ; Phimosis ; surgery ; Surgical Staplers ; Treatment Outcome

BACKGROUNDLiver transplantation is the most effective treatment for patients with end-stage liver failure, however infection after transplantation is a serious clinical complication. The purpose of this research was to investigate the molecular epidemiology and the influence of multidrug-resistant Gram-positive infection in patients, following liver transplantation, to provide reference for clinical treatment and prevention of Gram-positive bacterial infection.

METHODSWe isolated and detected bacteria from phlegm, throat swabs, urine, wound or wound secretions, blood, and fecal samples from 221 liver transplant patients in our hospital from January 2007 to April 2010. All isolated bacterial strains were identified and tested by minimal inhibitory concentration (MIC) drug-sensitive detection using the BioMerieux ATB bacterial identification instrument and repetitive extragenic palindromic-polymerase chain reaction (REP-PCR) detection of bacterial homology. Risk factors were calculated by multivariate Logistic regression analysis.

RESULTSWe collected 250 specimens from 221 patients hospitalized following liver transplantation surgery, of which 29 patients developed multiple infections. Sixty-five Gram-positive bacterial strains were isolated from different specimens from 53 infectious patients. We detected 29 multidrug-resistant Gram-positive strains from 29 patients (44.62%), including 20 Staphylococcus aureus (S. aureus) strains (68.97%) and nine Enterococcus strains (31.03%). All 20 S. aureus strains were highly resistant to aminoglycosides (gentamicin), cephalosporins (cefoxitin), quinolones (ciprofloxacin and levofloxacin), lincomycins (clindamycin), penicillin, and erythromycin. The resistance rate reached 100% in some cases. The S. aureus strains were highly sensitive to vancomycin and oxazolidinone (linezolid), with MIC50 < 2 µg/ml for both. The nine Enterococci strains were also highly resistant to aminoglycosides, quinolones, and penicillins, and highly sensitive to vancomycin (MIC50 < 2 µg/ml) and oxazolidinone (MIC50 < 1 µg/ml). Using REP-PCR detection, S. aureus was divided into five genotypes with 14 B-type strains. Enterococcus was divided into 11 genotypes, with two D-type strains, two G-type strains, and two K-type strains. The risk factors for Gram-positive bacterial infection in patients following liver transplantation were preoperative use of antibiotics (OR = 3.949, P = 0.004), high intra-operative blood input (OR = 1.071, P = 0.005), and postoperative renal failure (OR = 5.427, P = 0.043).

CONCLUSIONSS. aureus and Enterococcus were the main pathogens causing infection following liver transplantation in patients with drug-resistant Gram-positive bacterial infection. The isolated strains were resistant to multiple antibiotics. B-type S. aureus strains were predominant. Reasonable use of antibiotics, decreasing intra-operative blood input, and preventing post-operative renal failure may reduce Gram-positive bacterial infections and the appearance of drug-resistant strains following liver transplantation.

Adult ; Anti-Bacterial Agents ; pharmacology ; Drug Resistance, Multiple, Bacterial ; genetics ; Female ; Gram-Positive Bacteria ; drug effects ; genetics ; pathogenicity ; Gram-Positive Bacterial Infections ; epidemiology ; etiology ; microbiology ; Humans ; Liver Transplantation ; adverse effects ; Male ; Microbial Sensitivity Tests ; Middle Aged ; Molecular Epidemiology

OBJECTIVESTo study the clinical characteristics of hepatic failure with aspergillosis.

METHODSThe data of hepatic failure patients with fungal infection hospitalized in our hospital form January 1985 to June 2006 were collected. This research mainly focused on the clinical characteristics of the patients co-infected with aspergillosis.

RESULTSThe occurrence of aspergillosis was 20.5% (104 cases) among 507 hepatic failure patients with fungal infection. Compared with other fungal infection in hepatic failure patients, the effective rate of antifungal therapy and the improvement rate of underlying disease were worse in patients with aspergillus infection (36.5% vs 57.8%, P = 0.000; 26.0% vs 36.7%, P = 0.049). Aspergillus fumigatus was the most common species among 108 fungal species. The species next to Aspergillus fumigatus were Aspergillus niger and Aspergillus flavus. The mainly infected organ was lung and its clinical manifestation was atypical. Liver function could be improved with effective anti-fungus therapy.

CONCLUSIONSDiagnosis and treatment of aspergillosis is difficult in hepatic failure patients co-infected with aspergillosis. Early and effective antifungal therapy is helpful to the recovery of liver function in the hepatic failure patients suspected with aspergillosis co-infection.

Antifungal Agents ; therapeutic use ; Aspergillosis ; diagnosis ; drug therapy ; Aspergillus ; isolation & purification ; Humans ; Liver Failure ; diagnosis ; drug therapy ; microbiology

OBJECTIVETo study the efficacy of interleukin-10 (IL-10) in the treatment of experimental autoimmune prostatitis (EAP) and its effect on the expressions of tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta1 (TGF-beta1 ) in EAP rat models.

METHODSThirty Wistar rats were equally and randomly divided into a control, an EAP and an IL-10 group. The controls were treated with normal saline, the EAP models were made by injection of purified prostate protein twice with immune adjuvant, and the IL-10 group included the EAP models subjected to IL-10 intervention. The infiltration of the inflammatory cells of the prostate tissue was detected by HE staining, the ultrastructure of the prostate cells and their surrounding cells observed by electron microscopy, and the levels of TNF-alpha and TGF-beta1 in the three groups determined by semi-quantitative RT-PCR assay.

RESULTSThe EAP group showed significantly severer inflammation and higher levels of TNF-alpha and TGF-beta1 in the prostate tissue than the controls (P < 0.05). The IL-10 group exhibited significantly lessened inflammatory infiltration of the prostate tissue and decreased levels of TNF-alpha and TGF-beta as compared with the EAP group (P < 0.05).

CONCLUSIONIL-10 could relieve inflammatory infiltration of the prostate tissue and inhibit the expressions of TNF-alpha and TGF-beta1 in EAP rats, which is suggestive of its therapeutic efficacy for autoimmune prostatitis.

Animals ; Autoimmune Diseases ; complications ; drug therapy ; metabolism ; Disease Models, Animal ; Interleukin-10 ; therapeutic use ; Male ; Prostatitis ; drug therapy ; etiology ; metabolism ; Rats ; Rats, Wistar ; Transforming Growth Factor beta ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism