Objective:To investigate the impact of controlled ovarian hyperstimulation(COH) on thyroid function in infertile subclinical hypothyroidism (SCH) women treated with levothyroxine (LT4).Methods:A cohort study included LT4 treated infertile SCH patients undergoing in vitro fertilization/intracytoplasmic sperm injection and embryo transfer (IVF/ICSI-ET) from January 2015 to March 2017 in the Reproductive Center of the First People's Hospital of Yunnan Province were retrospectively analyzed. Finally, the paired thyroid hormone data of 124 patients before and after COH were included for statistical analysis. According to baseline thyroid stimulating hormone (TSH) level, women were divided into 0.2 mU/L≤TSH≤2.5 mU/L group ( n=88) and 2.5 mU/L4.2 mU/L, 77.8% (28/36) vs. 40.9% (36/88), P<0.001]. At the 14th day after ET, the TSH level in the clinical pregnancy group was significantly higher than that in the non-pregnancy group [6.09(2.18, 9.09) mU/L vs. 1.84(0.62, 4.45) mU/L, P=0.002]. The increment of TSH level was more significant in ATA positive group compared with ATA negative group after COH ( P=0.013). Conclusion:COH had a significant effect on serum TSH in LT4 treated infertile SCH women. The possible influence factors may include the baseline TSH level, status of ATA and pregnancy.

Objective:To investigate the impact of controlled ovarian hyperstimulation(COH) on thyroid function in infertile subclinical hypothyroidism (SCH) women treated with levothyroxine (LT4).Methods:A cohort study included LT4 treated infertile SCH patients undergoing in vitro fertilization/intracytoplasmic sperm injection and embryo transfer (IVF/ICSI-ET) from January 2015 to March 2017 in the Reproductive Center of the First People's Hospital of Yunnan Province were retrospectively analyzed. Finally, the paired thyroid hormone data of 124 patients before and after COH were included for statistical analysis. According to baseline thyroid stimulating hormone (TSH) level, women were divided into 0.2 mU/L≤TSH≤2.5 mU/L group ( n=88) and 2.5 mU/L4.2 mU/L, 77.8% (28/36) vs. 40.9% (36/88), P<0.001]. At the 14th day after ET, the TSH level in the clinical pregnancy group was significantly higher than that in the non-pregnancy group [6.09(2.18, 9.09) mU/L vs. 1.84(0.62, 4.45) mU/L, P=0.002]. The increment of TSH level was more significant in ATA positive group compared with ATA negative group after COH ( P=0.013). Conclusion:COH had a significant effect on serum TSH in LT4 treated infertile SCH women. The possible influence factors may include the baseline TSH level, status of ATA and pregnancy.

The study was established an HPLC method for simultaneous determination of 8 steroidal saponins (polyphyllins Ⅶ, H, Ⅵ, Ⅱ, Ⅰ, and Ⅴ, dioscin, and gracillin) in Paridis Rhizoma, and made an evaluation by determining steroidal saponins in 15 kinds of genus Paris. The analysis was performed on a Waters Acquity H-ClassTM UPLC ultrafine liquid chromatography system coupled with a PDA detector. The chromatographic separation was achieved through a CAPCELL PAK ADME (4.6 mm× 250 mm, 5 μm) column and the optimal mobile phase consisted of acetonitrile and water. The column was maintained at 21 ℃, and the flow rate was 0.8 mL•min ⁻¹. The UV detection wavelength was 203 nm. The results showed that ① the detected components can be well separated and all with good correlation coefficients. The standard calibration curves were linearly good (R2>0.999 9). The linearity was obtained over 0.041 70-3.812 00 μg. The average recoveries ranged from 95.91% to 103.8%. ② there are significant differences in the content of steroidal saponins from different species. The steroidal saponins are low content or almost none in P. mairei, P. polyphylla var. stenophylla, and P. delavayi have low content or almost did not contain, so these species are not suitable for medicinal use. The contents of steroidal saponins in P. polyphylla var. chinensis are varied from different places. There were high content of steroidal saponins in P. polyphylla var. yunnanensis, P. forrestii, P. daliensis, and P. axialis, even up to 5.0%, which indicated that they had the potential pharmic value of development.

Objective To study the clinicopathological and immunohistochemical features, histogenesis and biological behavior of solid pseudopapillary tumor of the pancreas ( SPT ). Methods Routine HE and immunohistochemical ( SP) methods were used in 20 cases of SPT. Results There were 18 females and 2 males, age ranging from 13 to 48 years with mean age of 25. 3 years. Abdominal pain and palpable mass were among the main complains. Seventeen cases were followed-up from 9 to 120 monthes. Fourteen cases were alive. Tumors were encapsulated, mixed with solid and cystic tissues. Histological features were psudopapillary structure with a fibrovascular core. Immunohistogically, the tumors were positive for a-1-AT ( 17 cases) , vimentin ( 14 cases) , synaptophysin ( 10 cases) , CgA (5 cases) ,CK and insulin (2 cases) ,glucagon and S-100 (1 case) ,PR (14 cases) , ER (1 case) ,pS2 (6 cases) , but all were negative for CEA and gastrin. Conclusion SPT is of low-graded malignancy and a distinct clinicopathologic entity in young female patients with both exocrine as well as endocrine differentiation. The tumor is closely related with sex hormone receptors.