Objective: To analyze the current status and influencing factors of postpartum depression symptoms among elderly multipara, so as to provide a basis for health management among elderly pregnant women. Methods: Multipara aged ≥35 years who underwent 42-day postpartum check-ups in Hangzhou Gynecology and Gynecology Hospital from October 2023 to October 2024 were selected as survey subjects using convenience sampling method. Demographic information, sleep quality and feeding methods data were collected using questionnaires. The postpartum depressive symptoms, parenting stress, and social support levels were assessed using the Chinese version of the Simplified Edinburgh Postnatal Depression Scale, Parenting Stress Inventory-Short Form, and Perceived Social Support Scale, respectively. Factors affecting postpartum depression symptoms among elderly multipara were analyzed through multivariable logistic regression model. Results: A total of 355 elderly multipara were surveyed. The average age was (38.19±2.10) years. There were 228 cesarean deliveries (64.23%) in this pregnancy. There were 21 cases (5.92%) of unplanned pregnancy and 21 cases (5.92%) of preterm birth. The parenting stress score was (72.56±11.08) points, and the social support score was (60.42±8.50) points. Postpartum depression symptoms was detected in 106 elderly multipara, with a detection rate of 29.86%. Multivariable logistic regression analysis showed that monthly household income per capita (≥5 000 yuan, OR=0.470, 95%CI: 0.281-0.785), unplanned pregnancy (yes, OR=2.619, 95%CI: 1.427-4.809), preterm delivery (yes,OR=4.105, 95%CI: 1.848-9.113), poor sleep quality (yes, OR=3.556, 95%CI: 1.659-7.595), feeding methods (mixed feeding, OR=1.483, 95%CI: 1.030-2.135; artificial feeding, OR=4.510, 95%CI: 2.018-10.070), parenting stress (OR=1.206, 95%CI: 1.162-1.441), and social support (OR=0.255, 95%CI: 0.111-0.420) were factors affecting postpartum depression symptoms among elderly multipara. Conclusion The detection rate of postpartum depression symptoms among elderly multiparas is relatively high, is associated with family economic status, pregnancy planning status, preterm delivery, sleep quality, feeding methods, parenting stress, and social support levels.

HuR (human antigen R), an mRNA-binding protein responsible for poor prognosis in nearly all kinds of malignancies, is a potential anti-tumor target for drug development. While screening HuR inhibitors with a fluorescence polarization (FP) based high-throughput screening (HTS) system, the clinically used drug eltrombopag was identified. Activity of eltrombopag on molecular level was verified with FP, electrophoretic mobility shift assay (EMSA), simulation docking and surface plasmon resonance (SPR). Further, we showed that eltrombopag inhibited cell proliferation of multiple cancer cell lines and macrophages, and the anti-tumor activity was also demonstrated in a 4T1 tumor-bearing mouse model. The data showed that eltrombopag was efficient in reducing microvessels in tumor tissues. We then confirmed the HuR-dependent anti-angiogenesis effect of eltrombopag in 4T1 cells and RAW264.7 macrophages with qRT-PCR, HuR-overexpression and HuR-silencing assays, RNA stability assays, RNA immunoprecipitation and luciferase assays. Finally, we analyzed the anti-angiogenesis effect of eltrombopag on human umbilical vein endothelial cells (HUVECs) mediated by macrophages with cell scratch assay and Matrigel angiogenesis assay. With these data, we revealed the HuR-dependent anti-angiogenesis effect of eltrombopag in breast tumor, suggesting that the existing drug eltrombopag may be used as an anti-cancer drug.

Objective To analysis the risk factors,clinical manifestations of adnexal torsion(AT) during pregnancy,providing evidence for the early diagnosis of AT in pregnancy.Methods Retrospective data were collected from 40 women who underwent surgery for AT during pregnancy between May 2006 and August 2016 at the first affiliated hospital of Wenzhou Medical University.In addition,94 women diagnosed with non-torsion adnexal during pregnancy were chosen to serve as control subjects.The risk factors and clinical characteristics of the two groups wcre compared.Results AT during pregnancy were likely to occurring within the 6th and 14th week of gestation.The rate of the adnexal masses with sizes between 5 and 8cm was significantly higher in torsion group(P ＜ 0.05).The rate of ovarian hyperstimulation syndrome (OHSS) was shown to be obviously elevated in the torsion group (P ＜ 0.05).The white blood cell counts,neutrophil ratio,the rate of lacking of intra-adnexal blood flow on color Doppler imaging were significantly elevated in torsion group (P ＜0.05).Conclusion The risk factors of AT during pregnancy may be occured within the 6th and 14th week of gestation,cyst sizes between 5 and 8cm and combined with OHSS.Adnexal tumors bearing higher risks for torsion and should be strongly considered for an aggressive strategy of management during pregnancy.Moreover,the combined detection of the white blood cell counts and neutrophil ratio as well as doppler imaging can contribute to the early diagnosis of AT during pregnancy.

OBJECTIVETo investigate the clinical significances and formation mechanism by analyzing the characteristics of the reverse phase nystagmus parameters from benign paroxysmal positional vertigo (BPPV) positioning test.

METHODSThere were 183 cases with BPPV, including 108 cases (59.0%) of posterior semicircular canal canalithiasis, 55 cases (30.1%) of horizontal semicircular canal canalithiasis, and 15 cases (8.2%) of horizontal and posterior semicircular canal cupulolithiasis and 5 cases (2.7%) of anterior semicircular canalithiasis . The video-nystagmography was utilized in positioning tests to induce nystagmus. The direction, intensity, time parameters characteristic of vertical nystagmus in Dix-Hallpike test and horizontal nystagmus in roll test were analyzed and compared.

RESULTSThere were no reversal phase nystagmus in 15 cases of semicircular canal cupulolithiasis and 5 cases of anterior semicircular canalithiasis. After the disappearance of vertical nystagmus which induced by hanging position (the first phase nystagmus) in 108 cases of posterior semicircular canalithiasis of Dix-Hallpike test, there was 54 cases(50.0%) of posterior semicircular canal canalithiasis displayed downward vertical nystagmus (reverse phase nystagmus) . The latency, duration time and intensity of the first phase nystagmus and reverse phase nystagmus were [(2.00 ± 1.10) s, (3.54 ± 1.42) s], [ (16.27 ± 4.95) s, (61.65 ± 33.69)s] and [ (51.80 ± 25.25) °/s, (10.65 ± 6.29)°/s] respectively; 43 cases(78.2%) of horizontal semicircular canal canalithiasis displayed the opposite to turning head (reverse phase nystagmus) after the horizontal nystagmus, similar with turning head disappeared in Roll test. The latency, duration time and intensity of the first phase of nystagmus and reverse phase nystagmus were [ (1.67 ± 1.07) s, (3.57 ± 1.89)s], [ (25.19 ± 9.74) s, (70.48 ± 40.26)s] and [ (68.47 ± 30.18) °/s, (11.22 ± 8.78)°/s] respectively. Comparing with the latency, duration time, intensity of first phase nystagmus and reverse phase nystagmus of posterior and horizontal semicircular canal canalithiasis, the differences had statistical significances (P < 0.05). Comparing with the first phase nystagmus of reverse phase and no reverse phase nystagmus canalithiasis, the difference of nystagmus intensity had statistical significances (P < 0.05); but the differences of latency and duration of nystagmus had no significant difference (P > 0.05).

CONCLUSIONSIt is common in PSC-Can and HSC-Can patients that reverse phase nystagmus is one of the clinical features of canalithiasis. It appears in side head position of Rolling test or the hanging of Dix- Hallpike test. More power of the first phase nystagmus has, reverse phase nystagmus will be induced much easier. In comparison of the reverse phase nystagmus, the first phase nystagmus has the shorter incubation and duration, but it has more power. It is helpful to avoid interruptions of clinical statolith positioning and reset since we recognize the reverse phase nystagmus. The mechanism might be similar to the vestibular mirror image nystagmus. It is another form of the vestibular mirror image nystagmus in BPPV patients.

Adult ; Aged ; Aged, 80 and over ; Benign Paroxysmal Positional Vertigo ; physiopathology ; Female ; Humans ; Male ; Middle Aged ; Nystagmus, Pathologic ; physiopathology ; Semicircular Ducts ; pathology ; Vestibular Function Tests

Objective To assess the association between Toll-like receptor 9 (TLR9) gene single nucleotide polymorphisms (SNPs) and systemic lupus erythematosus (SLE) development in Guangxi Zhuang and Han populations,as well as the difference in TLR9 SNPs between the two populations.Methods Totally,41 SLE patients of Zhuang nationality and 56 of Han nationality,as well as 82 healthy checkup examinees of Zhuang nationality and 120 of Han nationality were enrolled in this study.Venous blood samples were obtained from all of these subjects and subjected to DNA extraction.The single nucleotide polymorphisms in TLR9 gene were detected by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis followed by direct sequencing.Chi-square test and adjusted Chi-square test were conducted to assess the relationship between the genotype and allele frequencies of TLR9 SNPs and some clinical and laboratory parameters of patients with SLE,as well as the differences in genotype and allele frequencies of TLR9 SNPs between the two populations.Results The frequencies of CC,CT and TT genotypes of TLR9 SNP rs352140 were 42.9％,41.1％ and 16.1％ respectively in the patients of Han nationality,compared to 38.3％,55.8％ and 5.8％ in the healthy controls of Han nationality (all P ＜ 0.05),but no significant difference was observed in the frequency of C or T allele of the SNP rs352140 between the patients and controls of Han nationality (both P ＞ 0.05).There was no significant difference in the genotype or allele frequency of TLR9 SNP rs352140 between the patients and healthy controls of Zhuang nationality,or between the patients of Han nationality and Zhuang nationality (all P ＞ 0.05).The patients with anti-dsDNA antibodies showed a significantly higher frequency of TT genotype (P ＜ 0.05),but similar T allele frequency at TLR9 SNP rs352140 (P ＞ 0.05) compared with those without.The frequencies of both TT genotype and T allele of TLR9 SNP rs352140 were significantly increased in the patients with a SLE disease activity index (SLEDAI) ≥ 9 compared with those with a SLEDAI ＜ 9 (both P ＜ 0.05).There was no statistical difference in either the TT genotype or the T allele frequency at TLR9 SNP rs352140 between antinuclear antibody-positive and-negative patients with SLE (both P ＞ 0.05).Conclusions The TLR9 SNP rs352140 is correlated with several clinical and laboratory parameters of SLE,and might contribute to the susceptibility to SLE in Guangxi Han population.

Objective To investigate the clinical significances and formation mechanism by analyzing the characteristics of the reverse phase nystagmus parameters from benign paroxysmal positional vertigo ( BPPV ) positioning test.Methods There were 183 cases with BPPV , including 108 cases (59.0%) of posterior semicircular canal canalthasis , 55 cases ( 30.1%) of horizontal semicircular canal canalthasis, and 15 cases(8.2%) of horizontal and posterior semicircular canal cupulolithiasis and 5 cases (2.7%) of anterior semicircular canalithasis .The video-nystagmograph was utilized in positioning tests to induce nystagmus.The direction , intensity , time parameters characteristic of vertical nystagmus in Dix-Hallpike test and horizontal nystagmus in roll test were analyzed and compared.Results There were no reversal phase nystagmus in 15 cases of semicircular canal cupulolithiasis and 5 cases of anterior semicircular canalithasis .After the disappearance of vertical nystagmus which induced by hanging position ( the first phase nystagmus ) in 108 cases of posterior semicircular canalithasis of Dix-Hallpike test , there was 54 cases ( 50.0%) of posterior semicircular canal canalthasis displayed downward vertical nystagmus (reverse phase nystagmus ) .The latency, duration time and intensity of the first phase nystagmus and reverse phase nystagmus were [(2.00 ±1.10)s, (3.54 ±1.42)s], [(16.27 ±4.95)s, (61.65 ±33.69) s] and [ ( 51.80 ±25.25 ) °/s, ( 10.65 ±6.29 ) °/s ] respectively; 43 cases ( 78.2%) of horizontal semicircular canal canalithasis displayed the opposite to turning head ( reverse phase nystagmus ) after the horizontal nystagmus , similar with turning head disappeared in Roll test.The latency, duration time and intensity of the first phase of nystagmus and reverse phase nystagmus were [(1.67 ±1.07) s, (3.57 ± 1.89)s], [(25.19 ±9.74) s, (70.48 ±40.26) s] and [(68.47 ±30.18)°/s, (11.22 ±8.78)°/s] respectively.Comparing with the latency , duration time , intensity of first phase nystagmus and reverse phase nystagmus of posterior and horizontal semicircular canal canalithasis , the differences had statistical significances (P<0.05).Comparing with the first phase nystagmus of reverse phase and no reverse phase nystagmus canalithasis , the difference of nystagmus intensity had statistical significances ( P<0.05); but the differences of latency and duration of nystagmus had no significant difference ( P>0.05).Conclusions It is common in PSC-Can and HSC-Can patients that reverse phase nystagmus is one of the clinical features of canalithasis.It appears in side head position of Rolling test or the hanging of Dix-Hallpike test.More power of the first phase nystagmus has , reverse phase nystagmus will be induced much easier.In comparison of the reverse phase nystagmus , the first phase nystagmus has the shorter incubation and duration , but it has more power .It is helpful to avoid interruptions of clinical statolith positioning and reset since we recognize the reverse phase nystagmus.The mechanism might be similar to the vestibular mirror image nystagmus.It is another form of the vestibular mirror image nystagmus in BPPV patients.

Objective To discuss the clinical characteristics and treatment of amenorrhea caused by Tripterygium Wilfordii polyglycoside (TWP).Methods The sexual hormone levels of 25 outpatients of The First Affliated Hospital Of Wenzhou Medical College who suffered from secondary amenorrhea after the use of TWP were examined before and after the hormone therapy.In addition,the clinical presentations were observed.Results (1)The E2 level went down lower than 73.2pmol/L while FSH went up higher than 40U/L in all the 25 patients.Among them,24 patients were confirmed of the diagnosis of premature ovarian failure.⑵HT is an effective way for 20 patients.The total efficiency of the therapy was 80%.⑶The E2 secreted by ovary was higher (63.19?10.81)pmol/l vs. (365.45?161.60) pmol/L and FSH(110.72?21.52)U/L vs. 11.46?17.70)U/Lsecreted by pituitary was lower after therapy than before.⑷After the second period of therapy, the clinical perimenopausal symptoms of all the patients were improved to different degrees.Conclusion TWP may result in the disorders of ovarian functions, but and HT in time may be an effective therapy.