Objective:To evaluate the clinical value of CD4 + T lymphocyte subsets such as helper Th2 in patients with recurrent uveitis (BU) in Beh?et′s disease (BD). Methods:The clinical data of 153 hospitalized patients diagnosed with Beh?et′s disease from January 1, 2020 to June 30, 2023 in the Second Hospital of Shanxi Medical University were retrospectively analyzed. The subsets of CD4 + T lymphocytes were measured, including helper T cells (Th cells) such as Th1, Th2, Th17 and regulatory T cells (Treg cells), biochemical lipid indexes (TC, TG, etc.), the frequency of oral ulcers in the past 1 year, the frequency of genital ulcers in the past 1 year, and drug use before admission;According to whether there was ocular involvement and uveitis, 153 cases of BD were divided into Beh?et non-uveitis group (non-BU group) and Beh?et uveitis group (BU group). The above indexes and independent correlation factors of recurrent BU were compared between BU group and non-BU group;The above indexes and independent correlation factors of recurrent BU were compared between BU group and non-BU group. The levels of cytokines and ICBD total score, the correlation between ICBD total score and various cytokines, and the diagnostic performance of Th2 cells were compared between BU group and non-BU group.The statistical methods were Mann-Whitney U test, independent sample t test, Chi-square test, multiple logistic regression analysis, Pearson correlation analysis and receiver operating characteristic curve (ROC) analysis. Results:①The levels of Th1, Th2, Th17 cells, TC and TG in BU group were higher than those in non-BU group [133.87 (93.38, 229.87)/μl vs. 102.51(64.25, 149.23)/μl] and [9.43 (5.84, 14.13)/μl vs. 6.78(4.23, 9.44)/μl], [15.53 (9.36, 25.27)/μl vs. 9.83(5.46, 14.76)/μl], [4.21 (3.89, 4.90) mmol/L vs. 3.89(3.37, 4.34)mmol/L)], [1.43(1.00, 2.21)mmol/L vs. 0.96(0.69, 1.38)mmol/L], The differences were statistically significant ( Z=-3.24, Z=-3.05, Z=-3.94, Z=-2.25, Z=-3.47; all P<0.05); There was no statistical significance in Chi-square test between the two groups ( χ2=5.69, P>0.05).②The levels of IL-2, IL-10 and total ICBD score in BU group were higher than those in non-BU group, with statistical significance ( Z=-2.12, Z=-2.29, t=-6.48; all P<0.05). ③ The results of multiple logistic regression analysis showed that Th2 was an independent correlation factor for BU [ OR value (95% CI) was 1.143(1.007, 1.298), P=0.039]. The total score of BU patients was correlated with Th2 and Th17 cells. ROC analysis showed that the sensitivity of Th2 in diagnosing BU was 68.8%, the specificity was 49.5% and the area under the curve (95% CI) was 0.697 (0.585, 0.809) (P=0.001). Conclusion:CD4 + T lymphocyte subsets such as the absolute number of Th2 cells are related to BU, which is an important indicator to observe the severity of disease progression in BU patients, and has certain clinical value in evaluating the recurrence of BU in BD patients.

Objective:To investigate the value of antithrombin Ⅲ (AT-Ⅲ) in evaluating patients with decompensated hepatitis B liver cirrhosis and complicated with esophagogastric variceal bleeding (EVB).Methods:From January 1, 2018 to December 31, 2021, clinical data of 193 hospitalized patients with hepatitis B liver cirrhosis diagnosed in the Second Hospital of Shanxi Medical University were retrospectively analyzed, which included coagulation indicator (AT-Ⅲ), liver function indicators (total bilirubin, etc.), abdominal ultrasound results (portal vein diameter, portal vein blood flow velocity), and the occurrence of esophagogastric varices. According to the presence or absence of main complications, 193 patients with hepatitis B liver cirrhosis were divided into compensated group (60 cases) and decompensated group (133 cases). According to the presence or absence of EVB, 133 patients of decompensated group were divided into non-bleeding subgroup (96 cases) and bleeding subgroup (37 cases). The above indicators were compared among compensated group, decompensated group and their subgroups. The independent related factors of decompensated hepatitis B liver cirrhosis and EVB were analyzed. The level of AT-Ⅲ of each group were compared, and the relationship between AT-Ⅲ and Child-Pugh score was analyzed. The diagnostic capability of AT-Ⅲ in decompensated hepatitis B liver cirrhosis and complicated with EVB were analyzed. Mann-Whitney U test, independent sample t test, chi-square test, multiple logistic regression analysis, Pearson correlation analysis and receiver operating characteristic curve (ROC) analysis were used for statistical analysis. Results:The total bilirubin level of the decompensated group was higher than that of the compensated group, the portal vein diameter was larger than that of the compensated group, and the portal vein blood flow velocity was lower than that of the compensated group (31.50 μmol/L (21.90 μmol/L, 48.80 μmol/L) vs. 19.40 μmol/L (15.00 μmol/L, 25.50 μmol/L); (14.31±3.53) mm vs. (12.57±3.83) mm; (13.39±3.49) cm/s vs. (15.08±4.28) cm/s), and the differences were statistically significant ( Z=-5.76, t=-2.78 and 2.40; P<0.001, =0.006 and 0.018). The incidence of esophagogastric varices of the compensated group and the decompensated group was compared (40.0%, 24/60 vs. 87.2%, 116/133), and the difference was statistically significant ( χ2=64.06, P<0.001). The diameter of portal vein of the bleeding subgroup was larger than that of the non-bleeding subgroup, and the portal vein blood flow velocity was lower than that of the non-bleeding subgroup ((15.54±4.23) mm vs. (13.87±3.16) mm; (12.05±3.12) cm/s vs. (13.85±3.51) cm/s), and the differences were statistically significant ( t=-2.15 and 2.23, P=0.034 and 0.028). The AT-Ⅲ levels gradually decreased in the non-bleeding subgroup and bleeding subgroup of the compensated group and decompensated group, which were (79.52±16.02)%, (63.91±19.96)% and (35.92±13.69)%, respectively, the difference was statistically significant ( F=5.71, P=0.018). The AT-Ⅲ level of the compensated group was higher than that of the non-bleeding subgroup and the bleeding subgroup of the decompensated group, and the AT-Ⅲ level of the non-bleeding subgroup of the decompensated group was higher than that of the bleeding subgroup, and the differences were statistically significant ( t=5.11, 13.74 and 7.84, all P<0.001). The results of multivariate logistic regression analysis showed that total bilirubin and AT-Ⅲ were independent related factors of decompensation of hepatitis B liver cirrhosis ( OR (95% confidence interval (95% CI) 1.060 (1.018 to 1.104) and 0.945 (0.922 to 0.970), P=0.005 and <0.001). AT-Ⅲ was an independent related factor of decompensation of hepatitis B liver cirrhosis and complicated with EVB ( OR(95% CI) 0.902 (0.856 to 0.950, P<0.001). AT-Ⅲ was negatively correlated with Child-Pugh score ( r=-0.559, P<0.001). ROC analysis showed that the cut-off values of AT-Ⅲ in the diagnosis of decompensated stage of hepatitis B liver cirrhosis and complicated with EVB were 62.5% and 61.5%, the sensitivity was 88.3% and 89.2%, the specificity was 70.7% and 61.5%, and the area under the curve (95% CI) was 0.815 (0.755 to 0.874, P<0.001) and 0.899 (0.828 to 0.971, P<0.001), respectively. Conclusion:AT-Ⅲ is an important indicator in evaluating the severity of disease progression in patients with hepatitis B liver cirrhosis, and it has a certain clinical value in evaluating the bleeding tendency of patients with decompensated hepatitis B liver cirrhosis and complicated with esophagogastric varices.

Objective:This study aims to explore the structural characteristics of the abnormal brain cortex in patients with autism by analyzing the neuroanatomical differences between patients with autism and the healthy controls.Methods:This study analyzed the brain imaging data of patients with autism ( n=525) and healthy controls ( n=569) extracted from the database of the Autism Brain Imaging Data Exchange (ABIDE) which collected data from 20 sites around the world. The cerebral cortex thickness was estimated using the FreeSurfer software based on the data of brain structure and was compared between patients with autism and the healthy controls using the t test. At the same time, according to their age, all participants were divided into three groups, which were younger than 12 years old group(150 patients and 151 controls), 12 to 18 years old group (210 patients and 233 controls), and older than 18 years old group (159 patients and 183 controls), and the cortical thickness was compared between patients with autism and healthy controls in different age groups using the t test respectively. Results:Based on the comparison of the thickness of the cerebral cortex between the autism group and the control group, it was found that compared with the control group, patients with autism showed a significant increase in cortical thickness in the occipital face area of both left and right sides of the brain (left side: size=1 043.95 mm 2, Z=4.31, MNI coordinates: x=-13.1, y=-102.4, z=2.4; right side: size=1 364.13 mm 2, Z=5.14, MNI coordinates: x=14.4, y=-101.3, z=3.1) and significant atrophy of cortical thickness at the posterior part of the superior frontal gyrus on the right side of the brain (size=485.86 mm 2, Z=4.71, MNI coordinates: x=6.8, y=-13.1, z=61.6). Comparisons of cerebral cortical thickness in different age groups found that patients younger than 12 years old showed a significant reduction in cortical thickness in the middle and posterior right superior frontal gyrus (right side: size=914.44 mm 2, Z=4.86, MNI coordinates: x=19.7, y=32.4, z=41.1) and inferior temporal gyrus (left side: size=638.16 mm 2, Z=-4.36, MNI coordinates: x=-34.7, y=-32.5, z=-22.8) compared to the healthy controls of the same age. Patients within 12 to 18 years old showed a reduction in the cortical thickness of the posterior upper frontal gyrus and an increase in the occipital facial area compared to the corresponding healthy controls. No significant difference in the thickness of the cortex was found between patients older than 18 years and the healthy controls of the same age. Conclusion:Abnormal cortical thickness in the occipital face area and posterior part of the superior frontal gyrus could be the characteristics of neurodevelopment in patients with autism, especially in younger children with autism.

Objective:The study aims to explore the abnormal characteristics in cerebral cortex among children with different subtypes of attention deficit hyperactivity disorder (ADHD).Methods:Four hundred and twelve samples were obtained from the Healthy Brain Network project of American Child Mind Institute. There were 288 children with ADHD (all subjects: age,M=10.03,SD=3.11; 151 of ADHD-C, 20 of ADHD-H, and 117 of ADHD-I) and 124 healthy controls (age,M=9.98,SD=2.98). Using FreeSurfer software, we processed the brain structure images and obtained the cortical volume, cortical thickness and surface area for each subject. Analysis of Variance (ANOVA) and post hoc comparison analyses were conducted.Results:ANOVA analysis showed significant differences of the cortical volume located in the left superior parietal gyrus ( Z=5.94) and superior temporal gyrus ( Z=5.49) among the 3 subtypes of ADHD children and the healthy controls (Monte Carlo, P<0.05). Compared with the healthy controls, ADHD-H group exhibited an increased cortical volume in the left superior parietal gyrus ( Z=6.79), while the ADHD-I group had a decreased volume in the left superior temporal gyrus ( Z=-5.12) and lateral occipital cortex ( Z=-6.40). ADHD-C group also had a decreased volume in the left lateral occipital cortex ( Z=-3.37). Among 3 subtypes of ADHD patients, both ADHD-I and ADHD-C groups had a smaller volume in the left superior parietal gyrus than that of the ADHD-H group (ADHD-I: Z=-7.33,MNI coordinate:x=-26.8,y=-60.6,z=45.4; ADHD-C: Z=-7.14,MNI coordinate:x=-26.6,y=-60.2,z=45.4). Additionally, there was no statistical difference in cortical volume between the ADHD-I and ADHD-C group (Monte Carlo, P>0.05). Subsequent supplementary analyses showed that the sample size and age had no significant effect on the above results. Moreover, analysis of cortical thickness and the surface area showed that the abnormality of the cortical volume in different ADHD subtypes was mainly determined by the surface area of the cerebral cortex. Conclusion:Cortical measures in the superior parietal gyrus might be the crucial features that distinguishes the different subtypes of ADHD. These results enable us to further explore the neurodevelopmental mechanism of ADHD and guide the precise and specific clinical treatment.

Objective:This study aims to explore the structural characteristics of the abnormal brain cortex in patients with autism by analyzing the neuroanatomical differences between patients with autism and the healthy controls.Methods:This study analyzed the brain imaging data of patients with autism ( n=525) and healthy controls ( n=569) extracted from the database of the Autism Brain Imaging Data Exchange (ABIDE) which collected data from 20 sites around the world. The cerebral cortex thickness was estimated using the FreeSurfer software based on the data of brain structure and was compared between patients with autism and the healthy controls using the t test. At the same time, according to their age, all participants were divided into three groups, which were younger than 12 years old group(150 patients and 151 controls), 12 to 18 years old group (210 patients and 233 controls), and older than 18 years old group (159 patients and 183 controls), and the cortical thickness was compared between patients with autism and healthy controls in different age groups using the t test respectively. Results:Based on the comparison of the thickness of the cerebral cortex between the autism group and the control group, it was found that compared with the control group, patients with autism showed a significant increase in cortical thickness in the occipital face area of both left and right sides of the brain (left side: size=1 043.95 mm 2, Z=4.31, MNI coordinates: x=-13.1, y=-102.4, z=2.4; right side: size=1 364.13 mm 2, Z=5.14, MNI coordinates: x=14.4, y=-101.3, z=3.1) and significant atrophy of cortical thickness at the posterior part of the superior frontal gyrus on the right side of the brain (size=485.86 mm 2, Z=4.71, MNI coordinates: x=6.8, y=-13.1, z=61.6). Comparisons of cerebral cortical thickness in different age groups found that patients younger than 12 years old showed a significant reduction in cortical thickness in the middle and posterior right superior frontal gyrus (right side: size=914.44 mm 2, Z=4.86, MNI coordinates: x=19.7, y=32.4, z=41.1) and inferior temporal gyrus (left side: size=638.16 mm 2, Z=-4.36, MNI coordinates: x=-34.7, y=-32.5, z=-22.8) compared to the healthy controls of the same age. Patients within 12 to 18 years old showed a reduction in the cortical thickness of the posterior upper frontal gyrus and an increase in the occipital facial area compared to the corresponding healthy controls. No significant difference in the thickness of the cortex was found between patients older than 18 years and the healthy controls of the same age. Conclusion:Abnormal cortical thickness in the occipital face area and posterior part of the superior frontal gyrus could be the characteristics of neurodevelopment in patients with autism, especially in younger children with autism.

Objective:The study aims to explore the abnormal characteristics in cerebral cortex among children with different subtypes of attention deficit hyperactivity disorder (ADHD).Methods:Four hundred and twelve samples were obtained from the Healthy Brain Network project of American Child Mind Institute. There were 288 children with ADHD (all subjects: age,M=10.03,SD=3.11; 151 of ADHD-C, 20 of ADHD-H, and 117 of ADHD-I) and 124 healthy controls (age,M=9.98,SD=2.98). Using FreeSurfer software, we processed the brain structure images and obtained the cortical volume, cortical thickness and surface area for each subject. Analysis of Variance (ANOVA) and post hoc comparison analyses were conducted.Results:ANOVA analysis showed significant differences of the cortical volume located in the left superior parietal gyrus ( Z=5.94) and superior temporal gyrus ( Z=5.49) among the 3 subtypes of ADHD children and the healthy controls (Monte Carlo, P<0.05). Compared with the healthy controls, ADHD-H group exhibited an increased cortical volume in the left superior parietal gyrus ( Z=6.79), while the ADHD-I group had a decreased volume in the left superior temporal gyrus ( Z=-5.12) and lateral occipital cortex ( Z=-6.40). ADHD-C group also had a decreased volume in the left lateral occipital cortex ( Z=-3.37). Among 3 subtypes of ADHD patients, both ADHD-I and ADHD-C groups had a smaller volume in the left superior parietal gyrus than that of the ADHD-H group (ADHD-I: Z=-7.33,MNI coordinate:x=-26.8,y=-60.6,z=45.4; ADHD-C: Z=-7.14,MNI coordinate:x=-26.6,y=-60.2,z=45.4). Additionally, there was no statistical difference in cortical volume between the ADHD-I and ADHD-C group (Monte Carlo, P>0.05). Subsequent supplementary analyses showed that the sample size and age had no significant effect on the above results. Moreover, analysis of cortical thickness and the surface area showed that the abnormality of the cortical volume in different ADHD subtypes was mainly determined by the surface area of the cerebral cortex. Conclusion:Cortical measures in the superior parietal gyrus might be the crucial features that distinguishes the different subtypes of ADHD. These results enable us to further explore the neurodevelopmental mechanism of ADHD and guide the precise and specific clinical treatment.

Objective:To investigate the relationship between antithrombin Ⅲ (AT-Ⅲ) levels and CHA2DS2-VASc scores to assess the thromboembolism risk in patients with non-valvular atrial fibrillation (NVAF), and to explore the value of AT-Ⅲ in the risk assessment of thrombosis in these patients.Methods:We enrolled patients diagnosed with NVAF (observation group) and non-atrial fibrillation (control group), hospitalized in Fuwai Hospital of Chinese Academy of Medical Sciences from October 2018 to June 2019, and assessed the two groups for AT-Ⅲ, protein C, protein S, and lipid levels including lipoprotein (a), three acyl glycerin (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C). Based on the CHA2DS2-VASc score, patients with NVAF and a score of less than 2 were assigned to the low-and middle-risk groups; the high-risk group consisted of patients with a score of 2 or more. The diagnostic performance of AT-Ⅲ was evaluated using receiver operating characteristic (ROC) curve analysis, and the risk factors for high CHA2DS2-VASc scores were analyzed using logistic regression.Results:Overall, 206 cases were enrolled in the observation group, including 54 women (26%; aged 59.85±11.06 years). The control group consisted of 76 cases, with 19 women (25%; aged 59.34±9.84 years). The two groups were gender ( χ2=0.043, P=0.836) and age ( t=0.352, P=0.725) matched. In the observation group, AT-Ⅲ activity (98.68%±11.37%) was significantly lower than that in the control group (110.87%±13.91%), demonstrating a statistically significant difference ( t=-6.841, P<0.001). In total, 102 cases (49.5%) were assigned to the high-risk group, with 104 cases (50.5%) in the low-and medium-risk groups. In the high-risk group, the AT-Ⅲ activity (93.67%±9.92%) was significantly lower than that in the low-and middle-risk groups (103.60%±10.56%), with a statistically significant difference observed ( t=6.953, P<0.001). In the high-risk group, protein C ［(94.34±26.61)% vs. (102.63±22.74)%］, TC ［(4.09±1.02) mmol/L vs. (4.69±0.97) mmol/L］, and LDL-C ［(2.18±0.83) mmol/L vs. (2.74±0.88) mmol/L］ levels were lower than those observed in the low-risk group (P<0.05). For NVAF screening, the AT-Ⅲ early warning threshold was 96.5%, and the area under the ROC curve was 0.746 (95% CI: 0.681-0.812, P<0.001). Based on logistic regression analysis, low AT-Ⅲ activity levels were an independent risk factor for high CHA2DS2-VASc scores in NVAF ( OR=7.282，95% CI: 3.098-17.117， P<0.001). Additionally, logistic regression analysis demonstrated that with increasing age ( OR=44.339, 95% CI: 15.207-129.276), lower levels of AT-Ⅲ ( OR=7.282, 95% CI: 3.098-17.117) and TC ( OR=4.349, 95% CI: 1.739-10.875), and higher CHA2DS2-VASc scores were observed for non-valvular AF ( P<0.05). Conclusion:A positive correlation exists between the CHA2DS2-VASc score and old age, low AT-Ⅲ activity, and low TC levels, indicating a high reference value for evaluating thrombosis in NVAF.

Scar contracture after burn on the back of hand can easily lead to the limitation of flexion function of fingers, which seriously affects daily life activities. Generally, comprehensive rehabilitation treatment is adopted for scar contracture on the back of hand, among which wearing braces is an effective treatment method. However, some braces will limit the normal finger joints or must wait until all the affected fingers heal before they can be worn, and the wearing operation is quite complicated. In order to solve these problems, the author designed and made a finger flexion band, which was used to stretch the patients with limited flexion of finger caused by scar contracture after burn on the back of hand, and achieved good therapeutic effect. According to the measured hand size, the finger flexion band is cut and spliced from the fabric commonly used in daily life. The finger flexion band is designed with finger sleeve, which will not limit the normal finger joints, can interfere with the healed finger in advance, fix the corresponding fingers better, and improve the treatment comfort, especially for children who do not cooperate with the braces wearing. This finger flexion band is simple to make, cheap, convenient to use, and suitable for clinical promotion.

Objective To study the expression and biological functions of RN181 in SMMC7721 cells,the retroviral vector was constructed.Methods Gene cloning techniques were used to construct pRetrox-TRE3G/RN1S1 recombinant vector.The regulating plasmid pRetroX-TRE3G/RN181 or the response plasmid of pRetroX-Tet3G were respectively cotansfected into GP2-293 cells with Envelope Vector plasmid to package retrovirus after routine identification.Both viruses co-infected target cells SMCC7721,and then were selected by G418 to obtain stable cell lines.The stable cell lines were induced by doxycycline (DOX),and then verified by RT-PCR and Western blotting.CCK-8 was used to evaluate the effect of RN181 on growth of SMMC7721 cells.Results We constructed the recombinant plasmid.Stable recombinant plasmid were verified by screening.And there were significant differences of RN181 between the induced and uninduced cell lines through RT-PCR and Western blot.Conclusions We have successfully constructed the inducible stable RN181 expression SMCC7721 cell,which can be used as an effective cell model to study the biological functions of RN181.We found RN181 could suppress the proliferation and invasion in SMMC7721 cells in vitro.

Antiradiation drugs, also known as radioprotective agents, can prevent humans from radiation injury,reduce the clinical symptoms of radiation sickness,promote early recovery and reduce morbidity or mortality.Early developments of such agents focused on thiol synthetic compounds, such as amifostine (WR 2721).This compound reduced mortality, but its disadvantages, such as large use and high toxicity, limited its use in clinical practice.To find a suitable radioprotective agent is crucial to reducing side effects induced by ionizing radiation and increasing survival rate in patients during radiotherapy.In this paper, the classification and mechanism of radioprotective agents are reviewed and future developments in this field are predicted.