For adult rats fed on the following six kinds of diets for about 80 days, intermediate metabolic substrates in serum, liver and skeletal muscle were determined. The components of feeding diet are as follows:
1. STANDARD DIEF : 18% protein, 100 oil & 65% dextrin.
2. HIGH PROTEIN-HIGH FAT DIET: 29% protein, 25% oil & 39% dextrin.
3. LOW PROTEIN-LOW FAT DIET: 9% protein, 2% oil & 82% dextrin.
4. STANDARD DIET modified with choline chloride & vitamin B, C & E.
5. HIGH PROTEIN-HIGH FAT DIET modified with choline chloride & vitamin B, C & E.
6. LOW PROTEIN-LOW FAT DIET modified with choline chloride & vitamin B, C & E. (reference : 1, 2, 3 ; due to National Institute of Nutrition)
As the results increase of body weight was the least 3rd diet group, and by addition of choline chloride (above 4th, 5th & 6th diet groups) it were generally inhibited. Especially the latter phenomenon was remarkably observed in 6th diet group. Although intermediate metabolic substrates in skeletal muscle were no difference from their control levels, triglyceride and cholesterol of liver reduced also in 4th, 5th & 6th diet group, and FFA level of it increased on contrary. The triglycerides of interscapular brown fat and white fat of epididymis reduced in 4th, 5th & 6th diet groups. Furthermore, increase of triglyceride, FFA and cholesterol in serum observed in above 4th, 5th & 6th diet group, which will suggest that they might be removed from brown or white fat and choline chloride might play an important role of lipid-releasing effect from those tissues.