Osteoarthritis is a common joint disease characterized by progressive degeneration of articular cartilage, leading to pain and dysfunction. Mesenchymal stem cells (MSCs) have emerged as a promising tool for the treatment of osteoarthritis due to their ability to attenuate inflammatory responses, promoting cartilage repair, and immunomodulation. Recent studies have further clarified the mechanism of MSCs in treating osteoarthritis, including direct differentiation into chondrocytes, secretion of anti-inflammatory factors, and regulation of immune response, etc. Meanwhile, researchers are conducting phase I or phase II clinical trials of MSCs in treating osteoarthritis, and positive results have been achieved. Bone marrow-derived MSCs have strong potential for multidirectional differentiation and can effectively alleviate inflammation and promote cartilage repair, but their proliferative and immunomodulatory capacities are relatively low; adipose-derived MSCs show strong anti-inflammatory effects but are slightly less efficient in cartilage regeneration; umbilical cord-derived MSCs show great potential for cartilage repair and immunomodulation and synovial-derived MSCs have excellent chondrogenesis and immunomodulation. MSCs from synovial membrane have excellent chondrogenic capacity but are difficult to obtain. Despite the current promising research results on MSCs for osteoarthritis, clinical issues such as the choice of MSC source, mode of administration, and long-term safety and efficacy still need to be further explored.

Osteoarthritis is a common joint disease characterized by progressive degeneration of articular cartilage, leading to pain and dysfunction. Mesenchymal stem cells (MSCs) have emerged as a promising tool for the treatment of osteoarthritis due to their ability to attenuate inflammatory responses, promoting cartilage repair, and immunomodulation. Recent studies have further clarified the mechanism of MSCs in treating osteoarthritis, including direct differentiation into chondrocytes, secretion of anti-inflammatory factors, and regulation of immune response, etc. Meanwhile, researchers are conducting phase I or phase II clinical trials of MSCs in treating osteoarthritis, and positive results have been achieved. Bone marrow-derived MSCs have strong potential for multidirectional differentiation and can effectively alleviate inflammation and promote cartilage repair, but their proliferative and immunomodulatory capacities are relatively low; adipose-derived MSCs show strong anti-inflammatory effects but are slightly less efficient in cartilage regeneration; umbilical cord-derived MSCs show great potential for cartilage repair and immunomodulation and synovial-derived MSCs have excellent chondrogenesis and immunomodulation. MSCs from synovial membrane have excellent chondrogenic capacity but are difficult to obtain. Despite the current promising research results on MSCs for osteoarthritis, clinical issues such as the choice of MSC source, mode of administration, and long-term safety and efficacy still need to be further explored.

Objective:To evaluate the accuracy of the domestic "Skywalker" surgical robot in implementing personalized lower limb alignment reconstruction scheme in total knee arthroplasty (TKA) and the short-term clinical outcome of robotic assisted TKA.Methods:From September 2020 to January 2021, the data of patients who received surgical robot assisted TKA in 5 clinical centers in China (Shanghai Ninth People's Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Southwest Hospital affiliated to Third Military Medical University, The Affiliated Hospital of Qingdao University, The First Affiliated Hospital of Chongqing Medical University and Yantai Yuhuangding Hospital, and Shanghai Ninth People's Hospital affiliated to Shanghai Jiao Tong University School of Medicine was the group leader) were prospectively collected and retrospectively analyzed. There were 24 males and 82 females with an average age of 67.6±7.3 years, (range 45-80 years); Average body mass index 26.42±4.31 kg/m 2, all the operation were performed by "Skywalker" surgical robot system according to preoperative design based on CT. The operation time, intraoperative blood loss, hospitalization days and postoperative complications were recorded, and the imaging indexes including hip-knee-ankle (HKA), lateral distal angle of femur (LDFA) and medial proximal angle of tibia (MPTA) measured before and after the operation, implant model indexes (preoperative planning implant model and postoperative implant model) and short-term clinical efficacy indexes [Western Ontario and McMaster Universities (WOMAC) osteoarthritis index] pain score, stiffness score, joint function score, total score and SF-12 score before and 3 months after the operation) were compared. Results:The average follow-up period was 109.60±9.80 d, (range 95-143 d). The average operation time of 106 patients was 105.30±23.22 min; The average intraoperative blood loss was 141.70±58.33 ml; The average length of hospitalization was 5.82±2.80 d. One patient had ischemic stroke after operation, and one patient had abnormal liver function after operation. According to the judgment of the investigator, all of them were not related to the operation. The actual angle error is the difference between the preoperative planning angle and the postoperative measurement angle. The absolute error of 99.1% (105/106) of the HKA angle was within 3°, 90.8% (69/76) of LDFA, 98.7% (75/76) of the MPTA. In 45 patients in one center where data were available, the actual implant models used in all patients were consistent with the preoperative planning size, and there were only differences in version selection such as Asian condyle. WOMAC pain score, joint function score, total score was improved from 7.34±2.85, 25.10±9.85, 34.75±13.02 to 3.34±2.66, 14.68±9.64, 18.66±13.49 before and after operation, respectively, which were statistically significant ( P<0.001) and SF-12 physiological score and psychological score were improved form 27.24±6.42, 30.68±8.26 to 38.83±5.74, 39.36±7.85 before and after operation, respectively, which were statistically significant ( t=7.33, P<0.001; t=4.53, P=0.043). Conclusion:Domestic surgical robot system "Skywalker" can assist the surgeon to achieve accurate and personalized reconstruction of lower limb alignment and achieve satisfactory short-term clinical outcomes. The long-term clinical outcomes of personalized reconstruction and survival rate of implant still need to be further studied.

This article reports a case of sinus node dysfunction induced by thalidomide in ulcerative colitis in the Affiliated Hospital of Qingdao University and discusses the drug-induced arrhythmias induced by thalidomide.

This article reports a case of sinus node dysfunction induced by thalidomide in ulcerative colitis in the Affiliated Hospital of Qingdao University and discusses the drug-induced arrhythmias induced by thalidomide.

Objective:To compare the numerical rating scales (NRS) and Oswestry disability index (ODI) of denosumab in Chinese postmenopausal osteoporosis patients after 3 months, and analyze the early adverse reactions to provide reference for clinical diagnosis and treatment.Methods:Using a prospective study method, 260 patients with postmenopausal osteoporosis who were outpatients and inpatients in the Second Affiliated Hospital of Soochow University from September 2020 to October 2021 were selected, and general information, including age, height, weight, bone mineral density, history of fragility fractures, and use of anti-osteoporosis drugs. All subjects received denosumab 60 mg subcutaneously, and were given calcium and vitamin D at the same time. Pain was scored by NRS before treatment and 3 months after treatment, and functional improvement was assessed by ODI.Results:After 3 months of denosumab treatment in postmenopausal women with osteoporosis, among patients with different age groups, different degrees of osteoporosis, history of fragility fractures, and history of use of anti-osteoporosis drugs, NRS score and ODI score were lower than those before treatment, and the difference was statistically significant ( P<0.05). In addition, in patients with a history of fragility fractures (mainly vertebral fractures), the NRS scores and the ODI score decreased more significantly, and the difference was statistically significant ( P<0.05); the NRS score and ODI score decreased more significantly in patients with severe osteoporosis than in patients with osteoporosis, and the difference was statistically significant ( P<0.05); the BMD value of lumbar spine was negatively correlated with the reduction of NRS score before and after treatment ( P=0.042). In this study, 260 patients had musculoskeletal pain in 6 (2.3%), fatigue in 5 (1.9%), rash in 4 (1.5%), urinary tract infection in 2 (0.7%), and dizziness in 2 (0.7%), 2 case of fever (0.7%), 1 case of hypocalcemia (0.4%), a total of 22 cases of adverse reactions were reported, and the overall adverse reaction rate was 8.5%. Conclusion:Denosumab can improve pain symptoms and functional disability early in the clinical application of Chinese postmenopausal women with osteoporosis, and the incidence of adverse reactions is low. Especially for postmenopausal female osteoporosis patients with severe osteoporosis, low lumbar spine bone density, and a history of fragility fractures (mainly vertebral fractures), the application effect is more significant.

Objective To explore the expression of circ_0006692 in NSCLC and its relation with clinicopathological characteristics and related mechanism. Methods We collected 50 pairs of NSCLC tissues and adjacent tissues. The expression of circ_0006692 was detected by qRT-PCR and its relation with clinicopathological features was analyzed. We constructed lung cancer A549 cell line with circ_0006692 overexpression and knockdown. Cell proliferation, migration and invasion were detected by MTS, colony forming, wound healing and Transwell invasion assays. qRT-PCR and Western blot were used to detect the effect of circ_0006692 expression change on EMT-related gene expression. Results The expression of circ_0006692 in NSCLC was significantly higher than that in adjacent tissues (P < 0.05). The expression level of circ_0006692 was closely related to tumor size, TNM stage and pulmonary membrane invasion (P < 0.05). circ_0006692 knockdown inhibited cell proliferation, invasion and metastasis, while its overexpression promoted cell proliferation, invasion and metastasis. circ_0006692 knockdown inhibited the expression of EMT-related genes CDH2 and MMP7 in A549 cells and promoted the expression of CDH1. Conclusion The upregulated expression of circ_0006692 in NSCLC is closely related to tumor size, TNM stage and pulmonary membrane invasion. circ_0006692 expression could regulate the proliferation, invasion, metastasis and EMT progression of lung cancer A549 cells.

Objective To examine the adaptability of Carver and White' s behavioral activation/in-hibition scales(BIS/BAS Scales)in a large sample of Chinese military-man,and to revise the instrument and validate its factor structure. Methods A total of 2 921 Chinese military-men completed four subscales:one for the Behavioral Inhibition System,and three for the Behavioral Approach System(drive,reward re-sponsiveness,fun seeking).Data were analyzed using exploratory and confirmatory factor analysis.The internal consistency of the subscales and the complete scale were examined. Results The findings did not support Carver and White's 4-factor structure which was validated in a sample of Chinese college students.A 3-factor structure(behavioral inhibition,reward responsiveness,drive)in Chinese military-man were extracted and validated using confirmatory factor analysis(CFA)(KMO=0.87,CFI=0.94,RMSEA=0.06).The Cronbach α of behavioral inhibition,drive and reward responsiveness were 0.78,0.71 and 0.67 respectively.The internal consistencies of behavioral inhibition and drive were higher than four-factor model of Chinese college students and the internal consistencies of drive and reward responsiveness were less than American young adults.Be-havioral inhibition and drive positively predicted depressive symptoms(βBIS=0.332,βDrive=0.076,P<0.001),and reward responsiveness negatively predicted depressive symptoms(βRRS=-0.140,P<0.001). Conclusion The BIS/BAS Scales(revised Chinese military version)has acceptable reliability and validi-ty,and can be utilized as a measurement tool for assessing the approach/avoidance motivation of Chinese ser-vicemen.

After the stratification of the normal glucose tolerance, the changes of insulin resistance and βcell function in the development of type 2 diabetes mellitus were investigated. A retrospective analysis on data of 275 cases with oral glucose insulin releasing tests. The area under the insulin curve (AUCINS ) 108. 43 mU/ L was taken as the critical value of diagnosis. Normal glucose tolerance subjects were divided into the NGT-a group(AUCINS<108. 43 mU/ L) and the NGT-b group(AUCINS≥108. 43 mU/ L). The plasma glucose, insulin, insulin sensitivity, and β cell function were compared among the 4 groups: NGT-a group (n=96), NGT-b group (n=49), prediabetes group (n=71), and type 2 diabetes mellitus group ( n = 59). Among the fasting insulin, 2 h insulin, AUCINS , early-phase insulin secretion index(△I30 / △G30), the ratio of total insulin area under curve, and total glucose area under curve, disposition index, homeostasis model assessment for insulin resistance, and Matsuda insulin sensitivity index, the relationship as follows: NGT-b group>prediabetes group>NGT-a group>type 2 diabetes mellitus group. The NGT-b group was always the highest, prediabetes group was lower, NGT-a group and type 2 diabetes mellitus group were the lowest, there were significant differences (all P<0. 05). Making the NGT-a group as the basic state, in the NGT-b group, β cell function has begun to appear compensation and insulin resistance, and β cell function compensation reached the peak, the β cell function in the prediabetes group was beginning to compensate for the deficiency, the function of β cell in type 2 diabetes mellitus group decreased further. These findings suggest that the development process of type 2 diabetes mellitus could be the following four stages according to the function of β cell: β cell function normal, β cell functional compensation, β cell function loss of compensation, and finally β cell function failure.

Objective To investigate the effect of folic acid intervention on plasma homocysteine (Hcy) metabolic changes and pulse wave velocity(PWV)in patients with type H hypertension. Methods Patients(hos-pitalized from March 2014 to December in our hospital)with H type hypertension were randomly divided into treat-ment group and control group randomly ,and were given routine antihypertensive drug therapy. Treatment group was given oral folic acid 0.8 mg,1 times a day,the control group was given placebo,1 times a day. All patient were treated for 12 months. Changes of plasma Hcy and PWV levels were observed. Results 432 patients(Han nationality)with type H hypertension were enrolled in this study with the age of 61.7 ± 13.6 years old and the ratio of men and women is 1.3:1. The most common diseases were coronary heart disease and type 2 diabetes mellitus. 2 groups were treated for a period of 12 months,with follow-up time from 6 to 10 months(average duration in 8 months). After treatment,the difference between plasma Hcy(Z=-7.63,P=0.000)and PWV(Z=-3.16,P=0.002)levels of the two groups were statistically significant. Conclusion Folic acid intervention can significantly reduce the level of plasma Hcy in patients with type H hypertension ,slow down the progression of atherosclerosis and reduce the risk factors of cardiovascular disease.