In order to explore whether the expression of CircFoxO3 is related to the proliferation and apoptosis of porcine myoblasts,this study transfected porcine myoblasts with CircFoxO3 over-expression vector and siRNA,and detected cell proliferation and apoptosis by EdU and Annexin V-mCherry fluorescence staining,and detected the expression of apoptosis-related genes by RT-qPCR.The results showed that after transfection with overexpression vector,the proportion of ap-optotic cells increased and the number of cells proliferation decreased.At the same time,the ex-pression of apoptosis-related genes Caspase-3,Caspase-8,Fas and FasL was significantly up-regula-ted by RT-qPCR(P＜0.05),and the expression of Bax was extremely significantly up-regulated(P＜0.01).The expression of Bcl-2 was significantly down-regulated(P＜0.05).After transfection with si-CircFoxO3,the apoptosis level decreased and proliferation ability increased.the expression of Bax and FasL genes was highly significantly down-regulated(P＜0.01),the expression of caspase-3 and Caspase-8 was significantly decreased(P＜0.05),and the expression of Bcl-2 was significantly increased(P＜0.05).CircFoxO3 expression inhibited proliferation of porcine myo-blasts,promoted the expression of apoptosis-related genes,and promoted apoptosis of myoblasts.

In order to explore whether the expression of CircFoxO3 is related to the proliferation and apoptosis of porcine myoblasts,this study transfected porcine myoblasts with CircFoxO3 over-expression vector and siRNA,and detected cell proliferation and apoptosis by EdU and Annexin V-mCherry fluorescence staining,and detected the expression of apoptosis-related genes by RT-qPCR.The results showed that after transfection with overexpression vector,the proportion of ap-optotic cells increased and the number of cells proliferation decreased.At the same time,the ex-pression of apoptosis-related genes Caspase-3,Caspase-8,Fas and FasL was significantly up-regula-ted by RT-qPCR(P＜0.05),and the expression of Bax was extremely significantly up-regulated(P＜0.01).The expression of Bcl-2 was significantly down-regulated(P＜0.05).After transfection with si-CircFoxO3,the apoptosis level decreased and proliferation ability increased.the expression of Bax and FasL genes was highly significantly down-regulated(P＜0.01),the expression of caspase-3 and Caspase-8 was significantly decreased(P＜0.05),and the expression of Bcl-2 was significantly increased(P＜0.05).CircFoxO3 expression inhibited proliferation of porcine myo-blasts,promoted the expression of apoptosis-related genes,and promoted apoptosis of myoblasts.