Objective:To investigate the expression levels of long non-coding RNA (lncRNA) TCF7 and LSINCT5 in non-small cell lung cancer (NSCLC) tissues and the relationship with clinical pathological characteristics and prognosis of patients.Methods:NSCLC tissues and para-carcinoma tissues specimens of 108 NSCLC patients who underwent single-port thoracoscopic radical resection at Xiaogan Hospital Affiliated to Wuhan University of Science and Technology from June 2020 to October 2021 were collected. Real-time fluorescence quantitative PCR was applied to detect the expression of lncRNA TCF7 and LSINCT5 in NSCLC tissues and para-carcinoma tissues. The relationship between the expression of both and the clinical and pathological characteristics of patients was analyzed. Pearson correlation analysis was used to explore the correlation between the expression of lncRNA TCF7 and LSINCT5 in NSCLC tissues. Kaplan-Meier method was used for survival analysis. Cox proportional hazard regression model was applied to analyze the influencing factors of prognosis of NSCLC patients.Results:The relative expression of lncRNA TCF7 in NSCLC tissues was 1.62±0.53, which was significantly higher than that in para-carcinoma tissues (1.08±0.34, t=8.91, P<0.001). The relative expression of lncRNA LSINCT5 in NSCLC tissues was 1.54±0.48, which was significantly higher than that in para-carcinoma tissues (1.07±0.33, t=8.39, P<0.001). Pearson correlation analysis showed that the expression of lncRNA TCF7 and LSINCT5 in NSCLC tissues was positively correlated ( r=0.41, P<0.001). There were statistically significant differences in the expression of lncRNA TCF7 and LSINCT5 in NSCLC tissues among patients with different TNM stages ( χ2=6.28, P=0.012; χ2=5.40, P=0.020) and histological grades ( χ2=6.31, P=0.012; χ2=7.23, P=0.007). The 2-year survival rate of NSCLC patients with high expression of lncRNA TCF7 ( n=54) was 48.15%, which was significantly lower than that of patients with low expression ( n=54) (72.22%, χ2=6.53, P=0.011). The 2-year survival rate of NSCLC patients with high expression of lncRNA LSINCT5 ( n=55) was 47.23%, which was significantly lower than that of patients with low expression ( n=53) (73.58%, χ2=7.80, P=0.005). Univariate analysis showed that lymph node metastasis ( HR=1.55, 95% CI: 1.11-2.17, P=0.011), TNM stage Ⅲ ( HR=2.15, 95% CI: 1.32-3.48, P=0.002), poorly differentiated histologically grade ( HR=1.39, 95% CI: 1.11-1.73, P=0.004), positive lymph node status ( HR=1.75, 95% CI: 1.37-2.23, P<0.001), maximum diameter of tumor >2 cm ( HR=1.93, 95% CI: 1.09-3.43, P=0.024), high expression of lncRNA TCF7 (≥1.62) ( HR=1.77, 95% CI: 1.41-2.21, P<0.001), high expression of lncRNA LSINCT5 (≥1.54) ( HR=1.54, 95% CI: 1.21-1.97, P<0.001) were associated with prognosis of NSCLC patients who underwent single-port thoracoscopic radical resection. Multivariate analysis showed that TNM stage Ⅲ ( HR=1.25, 95% CI: 1.03-1.53, P=0.026), poor differentiated histologically grade ( HR=1.63, 95% CI: 1.07-2.48, P=0.023), high expression of lncRNA TCF7 ( HR=1.29, 95% CI: 1.03-1.62, P=0.025), high expression of lncRNA LSINCT5 ( HR=1.48, 95% CI: 1.14-1.93, P=0.004) were independent risk factors for prognosis of NSCLC patients. Conclusion:The expressions of lncRNA TCF7 and LSINCT5 are up-regulated in NSCLC tissues, and their expressions are positively correlated. Patients with high expression of lncRNA TCF7 and LSINCT5, patients in TNM stage Ⅲ, and patients with poorly differentiated histologically grade have a high risk of poor prognosis.