Objective:To explore the prognostic value of the age-adjusted Charlson comorbidity index (ACCI) in patients with stage IIB cervical squamous cell carcinoma (CSCC) who received radical concurrent chemoradiotherapy (rCCRT).Methods:Clinical data of 115 patients with stage IIB CSCC who underwent rCCRT at the First Affiliated Hospital of Xinxiang Medical University from January 2017 to January 2023 were retrospectively analyzed. Fourteen clinical factors, including ACCI, were assessed. Univariate and multivariate Cox regression analyses were performed to identify the independent prognostic factors for progression-free survival (PFS) and overall survival (OS). The optimal cut-off value for ACCI was determined using the receiver operating characteristic (ROC) curve analysis, and patients were divided into the high ACCI (ACCI > 3) and low ACCI (ACCI ≤ 3) groups. Survival differences between two groups were evaluated using Kaplan-Meier curves and compared by log-rank tests.Results:Multivariate Cox regression analysis revealed that ACCI was an independent prognostic factor for both PFS and OS ( HR=3.405, 95% CI: 1.108-10.467, P=0.032; HR=4.732, 95% CI: 1.363-16.425, P=0.014). Significant differences were observed in PFS and OS between the high and low ACCI groups ( P=0.023 and 0.003, respectively). The median PFS was 44 months in the high ACCI group and 56 months in the low ACCI group. The 2-year and 3-year PFS rates were 81.9% and 80.1% in the high ACCI group, and 94.4% and 94.4% in the low ACCI group, respectively. The median OS was 46 months in the high ACCI group and 56 months in the low ACCI group. The 2-year and 3-year OS rates were 88.3% and 84.7% in the high ACCI group, and 94.4% and 94.4% in the low ACCI group, respectively. Conclusions:For patients with stage IIB CSCC receiving rCCRT, ACCI is an independent and significant prognostic factor, with patients in the high ACCI group exhibiting worse prognosis.

Objective:To explore the prognostic value of the age-adjusted Charlson comorbidity index (ACCI) in patients with stage IIB cervical squamous cell carcinoma (CSCC) who received radical concurrent chemoradiotherapy (rCCRT).Methods:Clinical data of 115 patients with stage IIB CSCC who underwent rCCRT at the First Affiliated Hospital of Xinxiang Medical University from January 2017 to January 2023 were retrospectively analyzed. Fourteen clinical factors, including ACCI, were assessed. Univariate and multivariate Cox regression analyses were performed to identify the independent prognostic factors for progression-free survival (PFS) and overall survival (OS). The optimal cut-off value for ACCI was determined using the receiver operating characteristic (ROC) curve analysis, and patients were divided into the high ACCI (ACCI > 3) and low ACCI (ACCI ≤ 3) groups. Survival differences between two groups were evaluated using Kaplan-Meier curves and compared by log-rank tests.Results:Multivariate Cox regression analysis revealed that ACCI was an independent prognostic factor for both PFS and OS ( HR=3.405, 95% CI: 1.108-10.467, P=0.032; HR=4.732, 95% CI: 1.363-16.425, P=0.014). Significant differences were observed in PFS and OS between the high and low ACCI groups ( P=0.023 and 0.003, respectively). The median PFS was 44 months in the high ACCI group and 56 months in the low ACCI group. The 2-year and 3-year PFS rates were 81.9% and 80.1% in the high ACCI group, and 94.4% and 94.4% in the low ACCI group, respectively. The median OS was 46 months in the high ACCI group and 56 months in the low ACCI group. The 2-year and 3-year OS rates were 88.3% and 84.7% in the high ACCI group, and 94.4% and 94.4% in the low ACCI group, respectively. Conclusions:For patients with stage IIB CSCC receiving rCCRT, ACCI is an independent and significant prognostic factor, with patients in the high ACCI group exhibiting worse prognosis.

Skin flaps are frequently employed in plastic and reconstructive surgery to address tissue defects.However,their low survival rates remain a challenge,attributed to vascular crisis and necrosis.Despite numerous studies investigating drugs to alleviate flap necrosis,a comprehensive analysis of the research trend in this critical area is lacking.To gain a deeper understanding of the current status,research focal points,and future trends in drugs aimed at enhancing flap survival,a thorough retrospective analysis is imperative.This study aims to employ bibliometric methods to scrutinize the evolution,mechanisms,and forthcoming trends of drugs targeting flap survival improvement.Using VOSviewer software,we quantitatively and visually depict 1)annual temporal trends in the number of documents and citations;2)national/regional publications and their collaborations;3)institutional and authors'contribution;4)journal contribution and relevance;and 5)analysis of research hotspots and directions derived from keywords.Ultimately,we discussed the prospects and challenges of future advances and clinical trans-lation of drugs designed to enhance skin flap survival.In conclusion,the field of pharmacology dedicated to improving skin flap survival is expanding,and this study aims to offer a fresh perspective to promote the advancement and clinical application of such drugs.

Objective:To evaluate the clinical efficacy of kidney-invigorating and asthma-relieving granules in treating kidney deficiency type of bronchial asthma patients in persistent.Methods:A total of 100 patients with bronchial asthma admitted to Shanghai Municipal Hospital of TCM from March 2020 to August 2020 were selected as the research subjects. The patients were divided into control group and treatment group by random and double blind method, 50 in each group. Both groups were treated by routine basic treatment. The control group was treated with Kidney-invigorating and asthma-relieving Placebo, while the observation group was treated with kidney-invigorating and asthma-relieving granules. All the treatment lasted for 6 weeks. The TCM syndromes scores, Asthma Control Test (ACT) scores, Peak expiratory flow/predicted value (PEF%) and eosinophil in peripheral blood before and after treatment were observed.Results:A total of 91 patients completed the clinical study. There were 45 patients in the control group and 46 in the treatment group. The total effective rate in the treatment group was 93.5% (43/46), while that in the control group was 77.8% (35/45), and the difference was statistically significant ( χ2=4.579, P=0.032). After the treatment, the scores of integral efficacy on syndromes in Chinese medicine, ACT and PEF% in the treatment group were significantly higher than those in the control group ( t values were 2.802, 3.420 and 8.938, respectively, all Ps<0.01). The eosinophil in peripheral blood of patients in the treatment group was significantly lower than that of the control group ( t=3.481, P=0.001). Conclusion:On the basis of conventional treatment of western medicine, kidney-invigorating and asthma-relieving granules can relieve the clinical symptoms of asthma, improve the control level of asthma, enhance the level of PEF, reduce airway inflammation.

Objective:To evaluate the clinical efficacy of the Kechuansan acupoint plaster combined with acupoint injection of Chuankezhi in treating bronchial asthma. Methods:A total of 65 patients with bronchial asthma, who met the inclusion criteria in Shanghai Municipal Hospital of TCM from June 2018 to September 2018, were selected anddivided into control group and observation group by random number table method, 32 in the control group and 33 in the observation group. The control group was treated with acupoint injection of Chuankezhi, while the observation group was treated with acupoint plaster of Kechuansan combined with acupoint injection of Chuankezhi. Both groups were treated for 6 weeks. TCM syndromes and frequency of acute attackin one year before and after the treatment was scored and recorded. The patients' quality of life was assessed by Asthma Control Test (ACT) and the fractional exhaled nitric oxide (FeNO) level was measured by Sunvou-p100 nakurun breath analyzer to evaluate the clinical efficacy. Results:Through out the treatment, there were 3 dropouts in the observation group and 2 dropouts in the control group. 30 cases were completed in each group. The total effective rate in the observation group was 93.3% (28/30), and the control group was 73.3% (22/30). The difference was statistically significant ( χ2=4.320, P=0.038). After the treatment, the scores of TCM syndromes and ACT in the observation groups were significantly higher than those of the control group ( t values were 4.834 and 5.642, respectively, all Ps<0.01). The FeNO in the observation group were significantly lower than those in the control group ( t=3.180, P=0.020). The frequency of acute attack in one year in the observation group was less than that ofthe control group ( t=5.466, P<0.01). Conclusions:Acupoint plaster Kechuansan combined with acupoint injection of Chuankezhi can relieve the clinical symptoms of asthma, reduce the frequency of asthma attack, improve the control ability of asthma, reduce airway inflammation, which is more effective than using acupoint injection therapy alone.

Objective:To provide a reliable basis for the objective evaluation of the kinetic visual acuity (KVA) of navy pilots through a comprehensive analysis of the KVA test results among 190 navy pilots.Methods:We measured both the monocular and binocular KVA of 190 navy pilots at the Naval Medical Center with a DS-3 dynamic vision apparatus. The vision target velocity was set at 30 km/h. The hole-in-the card was also performed to examine the dominant eyes of the navy pilots.Results:The static visual acuity of all pilots tested was ≥1.0, but 6.05% of the monocular KVA and 4.74% of the binocular KVA were <1.0. There were no statistically significant differences in the binocular KVA ( P=0.07) and monocular KVA ( P=0.16) of the navy pilots of different age groups. The KVA of the left eyes was slightly higher than that those of the right eyes, but without statistical significance ( P=0.168). The KVA of the dominant eyes were slightly lower than the non-dominant eyes, but without statistical significance either ( P=0.284). The monocular KVA was significantly lower than the binocular KVA ( P<0.05). Conclusion:In the health exam for navy pilot recruitment and pilot health assessment, it is advisable for candidates/pilots to take a KVA test in addition to a static visual acuity test. The age of the examined navy pilots has no significant impact on their KVA. The monocular KVA is significantly lower than the binocular KVA. The KVA of the left eyes and the right eyes is relatively balanced, and the advantage of dominant eyes is not reflected in the KVA test.

Objective:To provide a reliable basis for the objective evaluation of the kinetic visual acuity (KVA) of navy pilots through a comprehensive analysis of the KVA test results among 190 navy pilots.Methods:We measured both the monocular and binocular KVA of 190 navy pilots at the Naval Medical Center with a DS-3 dynamic vision apparatus. The vision target velocity was set at 30 km/h. The hole-in-the card was also performed to examine the dominant eyes of the navy pilots.Results:The static visual acuity of all pilots tested was ≥1.0, but 6.05% of the monocular KVA and 4.74% of the binocular KVA were <1.0. There were no statistically significant differences in the binocular KVA ( P=0.07) and monocular KVA ( P=0.16) of the navy pilots of different age groups. The KVA of the left eyes was slightly higher than that those of the right eyes, but without statistical significance ( P=0.168). The KVA of the dominant eyes were slightly lower than the non-dominant eyes, but without statistical significance either ( P=0.284). The monocular KVA was significantly lower than the binocular KVA ( P<0.05). Conclusion:In the health exam for navy pilot recruitment and pilot health assessment, it is advisable for candidates/pilots to take a KVA test in addition to a static visual acuity test. The age of the examined navy pilots has no significant impact on their KVA. The monocular KVA is significantly lower than the binocular KVA. The KVA of the left eyes and the right eyes is relatively balanced, and the advantage of dominant eyes is not reflected in the KVA test.

Objective To explore the entrainment effect of different light regimens on the circadian rhythms of peripheral blood immune cells in mice .Methods One hundred and forty-four male C57BL/6 mice were kept under the 12 h-light and 12 h-dark cycle for 4 weeks.Then, the animals were randomly divided into 3 groups.The 24 animals in group 1 were treated with the former light regimen , the 60 animals in group 2 were kept in the light regimen of phase advance 12 hours, simulating eastward flight, and another 60 animals in group 3 were kept in the light regimen of phase delay 12 hours, simulating westward flight.Peripheral blood samples of the animals in group 1 were collected respectively one day before and 21 days after changes of light regimens, and blood samples of the animals in group 2 and 3 were collected on different days (i.e.day 2, 4, 7, 14 and 21) after changes of light regimens .Peripheral blood samples were taken at 4 different timepoints in 24 hours ( i.e.9 :00, 15:00, 21 :00 and 3 :00).Detections were made in immune cells , including the numbers of leukocytes, the percentage of lymphocytes , the percentage of CD4 +lymphocytes, the percentage of CD8 +lymphocytes, as well as monocyte and granulocyte counts .Finally, the circadian rhythms of differentimmune cells were evaluated in the animals of different groups .Results The immune cells in the C57BL/6 mice displayed different circadian rhythms under the 12 h-light and 12 h-dark cycle for 4 weeks.Under the new light regimens, the circadian rhythms of immune cells were entrained .It took 2 weeks for the animals of the simulated westward flight phase delay group to entrain the circadian rhythm , while the rate of entrainment for the animals of the simulated eastward flight phase delay group was 3 weeks.Conclusions Under the 12 h-light and 12 h-dark cycle, immune cells in the peripheral blood displayed a rise and fall circadian rhythm , which might facilitate the balance of the immune system .However, under the new light regimens, the circadian rhythms of different immune cells in the peripheral blood were entrained , with the entrainment rate of immune cells in the phase delay group being faster than that of the phase advance group .

Objective To explore the entrainment effect of different light regimens on the circadian rhythms of peripheral blood immune cells in mice .Methods One hundred and forty-four male C57BL/6 mice were kept under the 12 h-light and 12 h-dark cycle for 4 weeks.Then, the animals were randomly divided into 3 groups.The 24 animals in group 1 were treated with the former light regimen , the 60 animals in group 2 were kept in the light regimen of phase advance 12 hours, simulating eastward flight, and another 60 animals in group 3 were kept in the light regimen of phase delay 12 hours, simulating westward flight.Peripheral blood samples of the animals in group 1 were collected respectively one day before and 21 days after changes of light regimens, and blood samples of the animals in group 2 and 3 were collected on different days (i.e.day 2, 4, 7, 14 and 21) after changes of light regimens .Peripheral blood samples were taken at 4 different timepoints in 24 hours ( i.e.9 :00, 15:00, 21 :00 and 3 :00).Detections were made in immune cells , including the numbers of leukocytes, the percentage of lymphocytes , the percentage of CD4 +lymphocytes, the percentage of CD8 +lymphocytes, as well as monocyte and granulocyte counts .Finally, the circadian rhythms of differentimmune cells were evaluated in the animals of different groups .Results The immune cells in the C57BL/6 mice displayed different circadian rhythms under the 12 h-light and 12 h-dark cycle for 4 weeks.Under the new light regimens, the circadian rhythms of immune cells were entrained .It took 2 weeks for the animals of the simulated westward flight phase delay group to entrain the circadian rhythm , while the rate of entrainment for the animals of the simulated eastward flight phase delay group was 3 weeks.Conclusions Under the 12 h-light and 12 h-dark cycle, immune cells in the peripheral blood displayed a rise and fall circadian rhythm , which might facilitate the balance of the immune system .However, under the new light regimens, the circadian rhythms of different immune cells in the peripheral blood were entrained , with the entrainment rate of immune cells in the phase delay group being faster than that of the phase advance group .

Objective To investigate circadian rhythm of four genes (bmal1,clock,cry1,per1) in mouse peripheral white blood cells under the 12 h-light and 12 h-dark cycle (12L/12D) condition.Methods Forty-eight male mice were kept under the light regime of 12L/12D for four weeks.According to random number table,they were randomly and averagely divided into 6 groups.Then,the blood samples were taken and white blood cells were separated every four hours in a circadian day (9:00,13:00,17:00,21:00,1:00 and 5:00).The total RNA was extracted from the white blood cells and the mRNA level of each gene was quantified by real-time polymerase chain reaction (real-time PCR).The data were fitted by cosine function for getting the rhythm.The difference between apex and trough was analyzed by one-ANOVA.Results Under the light regime of 12L/12D,the four genes in peripheral white blood cells showed a significant circadian oscillation with different apex and trough.The apexes of four genes were higher than the troughs (P＜0.01).The equation fitted apex phase of clock gene was at about 5:00,compared to the apex time of the other three genes were at about 13:00-15:00.The apex time of clock mRNA was ahead of brnal1,and the apex and amplitude of it were higher than those of brnal1.However,the mRNAs of cry1 and per1 showed a similar apex time and apex level.Conclusions The findings demonstrate that the expression of the four genes in white blood cells shows an obvious circadian rhythm.It seems that clock plays more important role than bmal1 in the positive regulation,and cry1 and per1 play a similar role in negative regulation.This result will not only be helpful to understand the circadian fluctuation of immune function,but also to provide a target for implementing diagnosis and therapy of immune disorders.Further study is needed to elucidate the control effect of these genes in different immune cells in peripheral blood.