With the cooperation of Kurobe City, we conducted a basic survey using the nine classification of constitution and an intervention survey using a constitutional guidance program. The basic survey was conducted by the Kurobe group (Kurobe River alluvial fan spring water drinker n=155, 60.6±10.4 years old) and the non-Kurobe group (normal tap water drinker n=99, 50.7±12.8 living in the Hokuriku region without Kurobe City). A total of three surveys were conducted using the constitution Questionnaire (CCMQ-J). As a result, the Gentleness type, which is usually called the healthy constitution, was clearly higher in the Kurobe group, with 44.6 % in the Kurobe group and 22.5 % in the non-Kurobe group. In the proportion of each biased constitution(Mibyo constitution), the Kurobe group had less Wet-heat type (4.5 % vs 11.8 %), Qi-depression type (5.4 % vs 10.7 %), Yin-deficiency type (7.1 % vs 12.3 %) , and Phlegm-wetness type (5.8 % vs 9.6 %) than the non-Kurobe group. After the basic survey was completed, the Kurobe group was randomly divided into two groups, an intervention group (n=65, 62.3±9.3 years old) and a non-intervention group (n=68, 62.7±9.8 years old), and compared with the non-Kurobe group (control group) (n=80, 51.9±13.6 years old). The survey design was a simple comparative study, and the primary endpoint was a comparative study of changes in constitutional scores before and after intervention. As a result, in the Yang-deficiency type, a significant difference was observed between the intervention group, the non-intervention group and the control group at the first time after the intervention (One-way ANOVA p=0.04). No change was seen in the second survey after the intervention. In the comparison of Phlegm-wetness type between the three groups, there was a tendency in the amount of change in the constitutional score (One-way ANOVA p=0.087). From the above, it was suggested that the constitutional survey can not only grasp the health and the pre-symptomatic state, but also improve the pre-symptomatic by combining the guidance methods according to the constitution.

We evaluated the effect of Coix-seed Reactive Derivatives (CRD) on cold sensitivity of female hands and feet. Ninety females were assigned randomly to 3 groups and CRD(2g/day, 4g/day or placebo) was administrated for 8weeks. Significant improvement of cold sensitivity was observed in both the 2g/day and 4g/day groups. It was speculated that a naringenin and a quercetin as active ingredients of adlay hull may play a critical role in ameliorating effect on blood flow.

To clarify the functions of Coix-seed Reactive Derivatives(CRD), we observed 2 cases of plantar keratosis (case 1. 34 years of age, female/ case 2. 29 years of age, female) successfully cured by intake of CRD for several months. Although CRD intake seemed to be effective in these cases, further studies are needed to define the optimal dose and duration.

To clarify the functions of Coix-seed Reactive Derivatives(CRD), we observed 3 cases of refractory atopic dermatitis(case 1. 25 years of age, male/ case 2. 40 years of age, female/ case 3. 5 years of age, male) successfully cured by intake of CRD. Although CRD intake seemed to be effective in all cases, further studies are needed to define the optimal dose and duration.

To clarify the functions of Coix-seed Reactive Derivatives(CRD), we observed 2 cases of plantar wart (case 1. 8 years of age, female/ case 2. 26 years of age, female) successfully cured by intake of CRD for several months. Although CRD intake seemed to be effective in these cases, further studies are needed to define the optimal dose and duration.

In this report, we presented the case of a 45-year-old man who developed intractable eczema on the thumb-index interdigital fold for over 3 years. Coix-seed Reactive Derivatives(CRD) was administrated at a dose of 2.2g/day per day. The lesion cured markedly within 7 months. Although CRD intake seemed to be effective in this case, further studies are needed to define the optimal dose of this food.

The review reveals latest studies of Bangle that has unique ingredients, safety, healthy effect and especially neurotrophin activity. Banglenes have some neurotrophin activity such as neuritogenesis without NGF in vitro, property that enhances hippocampal neurogenesis and crosses the blood-brain barrier (BBB) in vivo. On the other hand, Java ginger bangle (Extract) improves spatial learning in Senescence-Accelerated Mouse. Moreover the human clinical trial for MCI (Mild Cognitive Impairment) have been just started.

Previous studies have demonstrated that Java ginger ( Zingiber purpureum) that is called ‘Bangle’ in Indonesia have some kinds of cognitive effects. In order to evaluate the safety of Java ginger bangle, we studied the genotoxicity by micronucleus test. The result shows that Java ginger bangle have no effect inducing appearance of micronucleus.

Ultraviolet (UV)-induced wrinkle and pigmentation are very common in clinical practice. In this report, we presented the case of a 58-year-old man who developed wrinkle and pigmentation around right hand, face and neck for ten years. Coix-seed Reactive Derivatives (CRD) was administrated orally 2.1g twice a day for 6 months. These lesions were improved in the third month, and a dramatic response was obtained at the end of 6 months of treatment. Further research is needed to define the optimal dose and duration of CRD intake.

Friction melanosis is a cutaneous entity characterized by a brownish pigmentation distributed on the skin over bony regions of various sites including a trunk or the limbs, after the use, for many years, of a nylon towel or scrub brush to clean the skin. Moreover, repeated trauma due to friction of clothes on the skin over bony prominences are another factors characterizing its pathogenesis. In this report, we presented the case of a 58-year-old man who developed severe friction melanosis around both knees and lower extremities for about 20 years. Coix-seed Reactive Derivatives (CRD) was administrated orally 2.1g twice a day for 6 months. The lesions of pigmentation diminished markedly within 3 months. Further research is needed to define the optimal dose and duration of CRD intake against friction melanosis.